Title:
RECOMBINANT STRAIN PRODUCING L-AMINO ACIDS, CONSTRUCTING METHOD THEREFOR AND METHOD FOR PRODUCING L-AMINO ACIDS.
Document Type and Number:
WIPO Patent Application WO/2015/120775
Kind Code:
A1
Abstract:
Provided is a recombinant strain producing L-amino acids, a constructing method therefor and a method for producing L-amino acids. Compared with the starting strains, the recombinant strain producing L-amino acids has decreased expression of 6-phosphoglucose isomerase Pgi and increased expression of 6-phosphogluconate dehydrogenase Zwf-OpcA. The starting strain can accumulate objective amino acids. Fermentation culturing of the recombinant strain can increase the yield of L-amino acids significantly. Also provided is a novel method for increasing the fermentation yield of L-amino acids, capable of being be used in bacterial fermentation of L-amino acids.
Inventors:
WEN TINGYI (CN)
SHANG XIULING (CN)
ZHANG YUN (CN)
LIU SHUWEN (CN)
LIANG YONG (CN)
ZHANG YU (CN)
SHANG XIULING (CN)
ZHANG YUN (CN)
LIU SHUWEN (CN)
LIANG YONG (CN)
ZHANG YU (CN)
Application Number:
PCT/CN2015/072220
Publication Date:
August 20, 2015
Filing Date:
February 04, 2015
Export Citation:
Assignee:
CHINESE ACAD INST MICROBIOLOGY (CN)
International Classes:
C12N1/21; C12P13/24; C12R1/15
Foreign References:
| CN1317049A | 2001-10-10 | |||
| EP2107128A2 | 2009-10-07 | |||
| CN102676509A | 2012-09-19 | |||
| CN101029310A | 2007-09-05 | |||
| CN102549144A | 2012-07-04 |
Other References:
ZHANG, YUN ET AL.: "Development and Application of an Arabinose-Inducible Expression System by Facilitating Inducer Uptake in Corynebacterium glutamicum", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 78, no. 16, 31 August 2012 (2012-08-31), pages 5831 - 5838, XP055312833, ISSN: 0099-2240
ZHANG, YUN ET AL.: "Genetic and Biochemical Characterization of Corynebacterium Glutamicum ATP Phosphoribosyltransferase and its Three Mutants Resistant to Feedback Inhibition by Histidine", BIOCHIMIE, vol. 94, no. 3, 31 March 2012 (2012-03-31), pages 829 - 838, XP055312834, ISSN: 0300-9084
ALLEN, S. ET AL.: "Metabolic Flux in Both the Purine Mononucleotide and Histidine Biosynthetic Pathways Can Influence Synthesis of the Hydroxymethyl Pyrimidine Moiety of Thiamine in Salmonella enterica", JOURNAL OF BACTERIOLOGY, vol. 184, 30 November 2002 (2002-11-30), pages 6130 - 6137, XP055312836, ISSN: 0021-9193
ZHANG, YUN ET AL.: "Genetic and Biochemical Characterization of Corynebacterium Glutamicum ATP Phosphoribosyltransferase and its Three Mutants Resistant to Feedback Inhibition by Histidine", BIOCHIMIE, vol. 94, no. 3, 31 March 2012 (2012-03-31), pages 829 - 838, XP055312834, ISSN: 0300-9084
ALLEN, S. ET AL.: "Metabolic Flux in Both the Purine Mononucleotide and Histidine Biosynthetic Pathways Can Influence Synthesis of the Hydroxymethyl Pyrimidine Moiety of Thiamine in Salmonella enterica", JOURNAL OF BACTERIOLOGY, vol. 184, 30 November 2002 (2002-11-30), pages 6130 - 6137, XP055312836, ISSN: 0021-9193
Attorney, Agent or Firm:
BEIJING LAWSING IP FIRM (CN)
北京律和信知识产权代理事务所（普通合伙） (CN)
北京律和信知识产权代理事务所（普通合伙） (CN)
Download PDF: