Title:
RECONSTITUTION OF DNA-END REPAIR PATHWAY IN PROKARYOTES
Document Type and Number:
WIPO Patent Application WO/2017/109167
Kind Code:
A3
Abstract:
Suggested is a method for engineering and/or editing the genome of prokaryotes encompassing the following steps: (i) providing a culture of prokaryotic cells, (ii) preparing a vector comprising an expression system encompassing at least one programmable DNA-binding and-cleaving protein, (iii) introducing said vector into said prokaryotic cells to target a specific DNA sequence in the genome of said prokaryotic cells.
Inventors:
PUL ÜMIT (DE)
MAMPEL JÖRG (DE)
ZUREK CHRISTIAN (DE)
REHDORF JESSICA (DE)
KROHN MICHAEL (DE)
MAMPEL JÖRG (DE)
ZUREK CHRISTIAN (DE)
REHDORF JESSICA (DE)
KROHN MICHAEL (DE)
Application Number:
PCT/EP2016/082551
Publication Date:
August 03, 2017
Filing Date:
December 23, 2016
Export Citation:
Assignee:
B R A I N AG (DE)
International Classes:
C12N15/10
Other References:
LI YIFAN ET AL: "Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing", September 2015, METABOLIC ENGINEERING, VOL. 31, PAGE(S) 13-21, ISSN: 1096-7176(print), XP002769483
LEE CIARAN M ET AL: "Development of Neisseria meningitidis CRISPR/Cas9 Systems for Efficient and Specific Genome Editing", MOLECULAR THERAPY, vol. 23, no. Suppl. 1, May 2015 (2015-05-01), & 18TH ANNUAL MEETING OF THE AMERICAN-SOCIETY-OF-GENE-AND-CELL-THERAPY (ASGCT); NEW ORLEANS, LA, USA; MAY 13 -16, 2015, pages S132 - S133, XP002769484
J.-H. OH ET AL: "CRISPR-Cas9-assisted recombineering in Lactobacillus reuteri", NUCLEIC ACIDS RESEARCH, vol. 42, no. 17, 29 September 2014 (2014-09-29), pages e131 - e131, XP055190221, ISSN: 0305-1048, DOI: 10.1093/nar/gku623
H. HUANG ET AL: "One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces", ACTA BIOCHIMICA ET BIOPHYSICA SINICA, vol. 47, no. 4, 3 March 2015 (2015-03-03), pages 231 - 243, XP055204421, ISSN: 1672-9145, DOI: 10.1093/abbs/gmv007
TAO XU ET AL: "Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 81, no. 13, 1 July 2015 (2015-07-01), pages 4423 - 4431, XP002741442, ISSN: 1098-5336, [retrieved on 20150424], DOI: 10.1128/AEM.00873-15
LEE CIARAN M ET AL: "Development of Neisseria meningitidis CRISPR/Cas9 Systems for Efficient and Specific Genome Editing", MOLECULAR THERAPY, vol. 23, no. Suppl. 1, May 2015 (2015-05-01), & 18TH ANNUAL MEETING OF THE AMERICAN-SOCIETY-OF-GENE-AND-CELL-THERAPY (ASGCT); NEW ORLEANS, LA, USA; MAY 13 -16, 2015, pages S132 - S133, XP002769484
J.-H. OH ET AL: "CRISPR-Cas9-assisted recombineering in Lactobacillus reuteri", NUCLEIC ACIDS RESEARCH, vol. 42, no. 17, 29 September 2014 (2014-09-29), pages e131 - e131, XP055190221, ISSN: 0305-1048, DOI: 10.1093/nar/gku623
H. HUANG ET AL: "One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces", ACTA BIOCHIMICA ET BIOPHYSICA SINICA, vol. 47, no. 4, 3 March 2015 (2015-03-03), pages 231 - 243, XP055204421, ISSN: 1672-9145, DOI: 10.1093/abbs/gmv007
TAO XU ET AL: "Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 81, no. 13, 1 July 2015 (2015-07-01), pages 4423 - 4431, XP002741442, ISSN: 1098-5336, [retrieved on 20150424], DOI: 10.1128/AEM.00873-15
Attorney, Agent or Firm:
FABRY, Bernd (DE)
Download PDF:
Previous Patent: NONWOVEN UNIT
Next Patent: USE OF A PROCESS FLUID WITH AN ENVIRONMENTALLY COMPATIBLE BIOSTABILIZER IN A GEOTHERMAL BOREHOLE
Next Patent: USE OF A PROCESS FLUID WITH AN ENVIRONMENTALLY COMPATIBLE BIOSTABILIZER IN A GEOTHERMAL BOREHOLE