Title:
REDUCTASE MUTANT FOR FORMING BIOPOLYMER
Document Type and Number:
WIPO Patent Application WO/2006/043555
Kind Code:
A1
Abstract:
A method of improving the ability to utilize a coenzyme and the catalytic activity of an enzyme, which is a wild type PhbB enzyme originating in Ralstonia eutropha or an enzyme having a sequential homology of at least 30% therewith, by mutating amino acid residues located at positions steric structurally equivalent to Met-12, Gly-13, Ala-32, Cys-34, Gly-35, Pro-36, Asn-37, Ser-38, Pro-39, Arg-40, Arg-41 and Ala-57 of the wild type PhbB enzyme. A method of producing a 3-hydroxyacyl-CoA and an optically active alcohol by using the PhbB enzyme mutant obtained by the above method as a catalyst. A method of producing a polyhydroxyalkanonic acid which involves the step of culturing and proliferating transformed cells containing a vector having a DNA encoding the above enzyme mutant or the step of reacting the enzyme mutant with a 3-ketoacyl-CoA.
Inventors:
MORIKAWA SOUICHI (JP)
OKUBO YUJI (JP)
NAKAI TAKAHISA (JP)
MATSUMOTO KEIJI (JP)
OKUBO YUJI (JP)
NAKAI TAKAHISA (JP)
MATSUMOTO KEIJI (JP)
Application Number:
PCT/JP2005/019125
Publication Date:
April 27, 2006
Filing Date:
October 18, 2005
Export Citation:
Assignee:
KANEKA CORP (JP)
MORIKAWA SOUICHI (JP)
OKUBO YUJI (JP)
NAKAI TAKAHISA (JP)
MATSUMOTO KEIJI (JP)
MORIKAWA SOUICHI (JP)
OKUBO YUJI (JP)
NAKAI TAKAHISA (JP)
MATSUMOTO KEIJI (JP)
International Classes:
C12N15/53; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/04; C12P7/04; C12P7/62; C12P19/32
Domestic Patent References:
| WO2003004653A1 | 2003-01-16 |
Foreign References:
| JP2002199890A | 2002-07-16 |
Other References:
LIEBERGESELL M AND STEINBUCHEL A.: "Cloning and nucleotide sequences of genes relevant for biosynthesis of poly(3-hydroxybutyric acid) in Chromatium vinosum strain D.", EUR J BIOCHEM., vol. 209, no. 1, 1992, pages 135 - 150, XP002995056
MATSUSAKI H ET AL: "Cloning and molecular analysis of the Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) biosynthesis genes in Pseudomonas sp. strain 61-3.", J BACTERIOL., vol. 180, no. 24, December 1998 (1998-12-01), pages 6459 - 6467, XP002167124
CHOI J I, LEE SY, HAN K.: "Cloning of the Alcaligenes latus polyhydroxyalkanoate biosynthesis genes and use of these genes for enhanced production of Poly(3-hydroxybutyrate) in Escherichiacoli.", APPL ANVIRON MICROBIOL., vol. 64, no. 12, December 1998 (1998-12-01), pages 4897 - 4903, XP001033932
MATSUSAKI H ET AL: "Cloning and molecular analysis of the Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) biosynthesis genes in Pseudomonas sp. strain 61-3.", J BACTERIOL., vol. 180, no. 24, December 1998 (1998-12-01), pages 6459 - 6467, XP002167124
CHOI J I, LEE SY, HAN K.: "Cloning of the Alcaligenes latus polyhydroxyalkanoate biosynthesis genes and use of these genes for enhanced production of Poly(3-hydroxybutyrate) in Escherichiacoli.", APPL ANVIRON MICROBIOL., vol. 64, no. 12, December 1998 (1998-12-01), pages 4897 - 4903, XP001033932
Attorney, Agent or Firm:
Yasutomi, Yasuo (4-20 Nishinakajima 5-chome, Yodogawa-k, Osaka-shi Osaka 11, JP)
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