Title:
REGULATING ORGANELLE HOMEOSTASIS IN A CELL FACTORY
Document Type and Number:
WIPO Patent Application WO2000071579
Kind Code:
A3
Abstract:
The present invention relates to a method for modulating the production of secondary metabolites in the fungus Penicillium chrysogenum by influencing the homeostasis of organelles by modulating a least one gene which is directly related to organelle homeostasis in this fungus. Preferably, the organelle homeostasis is modulated by genetically engineering at least one gene encoding a protein which is responsible for organelle biogenesis (the expression and/or activity of which is to be increased) and/or at least one gene encoding a protein which is responsible for organelle degeneration (the expression and/or activity of which is to be decreased). Genes involved in organelle biosynthesis in P. chrysogenum are exemplified by the so-called pex11 gene. Genes involved in organelle degradation are exemplified by the so-called vps15 and pdd1 genes.
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Inventors:
BOVENBERG ROELOF ARY LANS (NL)
VAN DEN BERG MARCO ALEXANDER (NL)
NIEBOER MAARTEN (NL)
VEENHUIS MARTEN (NL)
KIEL JAN ANDRIES KORNELIS WILL (NL)
VAN DER KLEI IDA JOHANNA (NL)
VAN DEN BERG MARCO ALEXANDER (NL)
NIEBOER MAARTEN (NL)
VEENHUIS MARTEN (NL)
KIEL JAN ANDRIES KORNELIS WILL (NL)
VAN DER KLEI IDA JOHANNA (NL)
Application Number:
PCT/EP2000/004718
Publication Date:
March 01, 2001
Filing Date:
May 22, 2000
Export Citation:
Assignee:
DSM NV (NL)
BOVENBERG ROELOF ARY LANS (NL)
DEN BERG MARCO ALEXANDER VAN (NL)
NIEBOER MAARTEN (NL)
VEENHUIS MARTEN (NL)
KIEL JAN ANDRIES KORNELIS WILL (NL)
KLEI IDA JOHANNA V D (NL)
BOVENBERG ROELOF ARY LANS (NL)
DEN BERG MARCO ALEXANDER VAN (NL)
NIEBOER MAARTEN (NL)
VEENHUIS MARTEN (NL)
KIEL JAN ANDRIES KORNELIS WILL (NL)
KLEI IDA JOHANNA V D (NL)
International Classes:
C07K14/37; C07K14/385; C12N1/19; C12N15/31; C12N15/80; C12P37/00; C12R1/82; (IPC1-7): C12N15/31; C07K14/385; C12N1/19; C12N15/80; C12P37/00
Domestic Patent References:
| WO1994024289A1 | 1994-10-27 | |||
| WO1997038107A1 | 1997-10-16 |
Foreign References:
| EP0448180A2 | 1991-09-25 |
Other References:
MARSHALL P ET AL: "Pmp27 promotes peroxisomal proliferation", J. CELL BIOL., vol. 129, no. 2, April 1995 (1995-04-01), pages 345 - 355, XP002120786
SAKAI Y ET AL: "The Candida boidinii peroxisomal membrane protein Pmp30 has a role in peroxisomal proliferation and is functionally homologous to Pmp27 from Saccharomyces cerevisiae", J BACTERIOL., vol. 177, December 1995 (1995-12-01), pages 6773 - 6781, XP002120787
ROGGENKAMP R ET AL: "Formation of irregular giant peroxisomes by overproduction of the crystalloid core protein methanol oxidase in the methylotrophic yeast Hansenula polymorpha", MOL CELL BIOL., vol. 9, no. 3, March 1989 (1989-03-01), pages 988 - 994, XP000037387
SAKAI Y ET AL: "The Candida boidinii peroxisomal membrane protein Pmp30 has a role in peroxisomal proliferation and is functionally homologous to Pmp27 from Saccharomyces cerevisiae", J BACTERIOL., vol. 177, December 1995 (1995-12-01), pages 6773 - 6781, XP002120787
ROGGENKAMP R ET AL: "Formation of irregular giant peroxisomes by overproduction of the crystalloid core protein methanol oxidase in the methylotrophic yeast Hansenula polymorpha", MOL CELL BIOL., vol. 9, no. 3, March 1989 (1989-03-01), pages 988 - 994, XP000037387
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