Title:
REPORTER SYSTEM FOR SCREENING VFM QUORUM SENSING SIGNAL QUENCHING BACTERIA AND INHIBITOR, AND CONSTRUCTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2023/279585
Kind Code:
A1
Abstract:
Provided are a reporter system for screening VFM quorum sensing signal quenching bacteria and an inhibitor, and a construction method therefor. Dickeya zeae MS2 is used as a recipient bacterium, into which a reporter plasmid is transferred to obtain the provided reporter system VR2, wherein the reporter plasmid is obtained by means of taking pPROBE-NT plasmid as a framework and inserting an optimized vfmE promoter upstream of a GFP gene. By means of taking a green fluorescent protein as a reporter gene, on the premise that a VFM signal structure is unknown, the system can be used for detecting the VFM signal intensity, which provides a new method for screening quorum quenching biocontrol bacteria and signal inhibitors of bacterial soft rot caused by Dickeya.
Inventors:
ZHOU JIANUAN (CN)
HU MING (CN)
ZHANG LIAN-HUI (CN)
LIU FAN (CN)
HU MING (CN)
ZHANG LIAN-HUI (CN)
LIU FAN (CN)
Application Number:
PCT/CN2021/126939
Publication Date:
January 12, 2023
Filing Date:
October 28, 2021
Export Citation:
Assignee:
UNIV SOUTH CHINA AGRICULT (CN)
International Classes:
C12N15/113; C12N1/21; C12N15/65; C12N15/66; C12N15/74; C12R1/01; G01N21/64
Foreign References:
| CN113416731A | 2021-09-21 | |||
| CN108624526A | 2018-10-09 | |||
| CN109022308A | 2018-12-18 |
Other References:
DATABASE Nucleotide 17 January 2018 (2018-01-17), ANONYMOUS: "Dickeya zeae strain MS2 chromosome, complete genome", XP055596106, retrieved from GenBank Database accession no. CP025799
XU JIAJING: "Isolation,Properties and Structural Characterization of VFM,a New Quorum Sensing Signal of Dickeya Dadantii", CHINESE MASTER’S DISSERTATIONS FULL-TEXT DATABASE, BASIC SCIENCES, 15 August 2020 (2020-08-15), pages 1 - 106, XP093022484
LV MINGFA, CHEN YUFAN, LIAO LISHENG, LIANG ZHIBIN, SHI ZURONG, TANG YINGXIN, YE SIXUAN, ZHOU JIANUAN, ZHANG LIANHUI: "Fis is a global regulator critical for modulation of virulence factor production and pathogenicity of Dickeya zeae", SCIENTIFIC REPORTS, vol. 8, no. 1, 10 January 2018 (2018-01-10), pages 1 - 13, XP093022318, DOI: 10.1038/s41598-017-18578-2
XU JIAJING: "Isolation,Properties and Structural Characterization of VFM,a New Quorum Sensing Signal of Dickeya Dadantii", CHINESE MASTER’S DISSERTATIONS FULL-TEXT DATABASE, BASIC SCIENCES, 15 August 2020 (2020-08-15), pages 1 - 106, XP093022484
LV MINGFA, CHEN YUFAN, LIAO LISHENG, LIANG ZHIBIN, SHI ZURONG, TANG YINGXIN, YE SIXUAN, ZHOU JIANUAN, ZHANG LIANHUI: "Fis is a global regulator critical for modulation of virulence factor production and pathogenicity of Dickeya zeae", SCIENTIFIC REPORTS, vol. 8, no. 1, 10 January 2018 (2018-01-10), pages 1 - 13, XP093022318, DOI: 10.1038/s41598-017-18578-2
Attorney, Agent or Firm:
GUANGZHOU ZHIFU INTELLECTUAL PROPERTY AGENCY (GENERAL PARTNERSHIP) (CN)
Download PDF: