Title:
RESTRICTED GLUCOSE FEED FOR ANIMAL CELL CULTURE
Document Type and Number:
WIPO Patent Application WO/2004/104186
Kind Code:
A1
Abstract:
Methods of improving protein production in animal cell cultures are provided. Cell culture methods are presented wherein glucose is fed in a restricted manner to cell culture; this restricted feeding of glucose to the cell culture results in lactate production being controlled to a low level. The restricted feeding of glucose in a fed-batch process is not accomplished through a constant-rate feeding of glucose, and the restricted feeding need not depend on sampling. Instead, restricted feeding of glucose to the culture is accomplished through feeding of glucose to the culture at a rate that is a function of an expected or a premodeled rate of glucose consumption by the animal cells when exposed to medium containing a high level of glucose. Because lactate production is controlled to low levels, recombinant protein production is increased.
Inventors:
DRAPEAU DENIS (US)
STANEK TERRY CARDOZA (US)
LUAN YEN-TUNG (US)
STANEK TERRY CARDOZA (US)
LUAN YEN-TUNG (US)
Application Number:
PCT/US2004/015597
Publication Date:
December 02, 2004
Filing Date:
May 17, 2004
Export Citation:
Assignee:
WYETH CORP (US)
DRAPEAU DENIS (US)
STANEK TERRY CARDOZA (US)
LUAN YEN-TUNG (US)
DRAPEAU DENIS (US)
STANEK TERRY CARDOZA (US)
LUAN YEN-TUNG (US)
International Classes:
C12N5/00; C12N5/02; (IPC1-7): C12N5/02
Domestic Patent References:
| WO2004048556A1 | 2004-06-10 | |||
| WO1998041611A1 | 1998-09-24 |
Attorney, Agent or Firm:
Mandra, Raymond R. (Cella Harper & Scinto, 30 Rockefeller Plaz, New York NY, US)
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