Title:
RNA ACTION INHIBITOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/039598
Kind Code:
A1
Abstract:
Provided is a target RNA action inhibitor based on RNA interference and ASO. An RNA interference agent of the present invention is provided with a guide strand and a passenger strand against the target RNA, wherein blunt ends are formed at both ends of double stranded RNA obtained through pairing of the passenger strand and the guide strand, and one or more units selected from the group consisting of units represented by formula (1) and formula (2) are provided to: (a) the 5' end side and the 3' end side of the passenger strand; and (b) the 3' end side of the guide strand. Formula (1) (wherein X represents an oxygen atom or a sulfur atom). Formula (2) (wherein X represents an oxygen atom or a sulfur atom).
Inventors:
ASANUMA HIROYUKI (JP)
KAMIYA YUKIKO (JP)
MURAYAMA KEIJI (JP)
TSUBOI TOSHIKI (JP)
DOKE TOMOHITO (JP)
ISHIMOTO TAKUJI (JP)
MARUYAMA SHOICHI (JP)
KOKURYO TOSHIO (JP)
KOTO AYAKO (JP)
ISHIMARU ISAO (JP)
ASAI RYOICHI (JP)
NAKASHA AYAKA (JP)
SUGITA TOMOE (JP)
NISHI KEISUKE (JP)
YUGUCHI MOTOKI (JP)
MATSUMOTO MASANORI (JP)
KAMIYA YUKIKO (JP)
MURAYAMA KEIJI (JP)
TSUBOI TOSHIKI (JP)
DOKE TOMOHITO (JP)
ISHIMOTO TAKUJI (JP)
MARUYAMA SHOICHI (JP)
KOKURYO TOSHIO (JP)
KOTO AYAKO (JP)
ISHIMARU ISAO (JP)
ASAI RYOICHI (JP)
NAKASHA AYAKA (JP)
SUGITA TOMOE (JP)
NISHI KEISUKE (JP)
YUGUCHI MOTOKI (JP)
MATSUMOTO MASANORI (JP)
Application Number:
PCT/JP2020/031513
Publication Date:
March 04, 2021
Filing Date:
August 20, 2020
Export Citation:
Assignee:
NATIONAL UNIV CORPORATION TOKAI NATIONAL HIGHER EDUCATION AND RESEARCH SYSTEM (JP)
NICCA CHEMICAL CO (JP)
HOKKAIDO SYSTEM SCIENCE CO LTD (JP)
NICCA CHEMICAL CO (JP)
HOKKAIDO SYSTEM SCIENCE CO LTD (JP)
International Classes:
A61P3/10; A61K31/7115; A61K31/713; A61P35/00; A61P43/00; C12N15/113
Domestic Patent References:
| WO2011105610A1 | 2011-09-01 |
Foreign References:
| JP2016130232A | 2016-07-21 | |||
| JP2019030312A | 2019-02-28 | |||
| JP2016130232A | 2016-07-21 | |||
| JP2011135824A | 2011-07-14 | |||
| JP2012170373A | 2012-09-10 | |||
| JP2013078298A | 2013-05-02 |
Other References:
KAMIYA, Y. ET AL.: "Enhancement of stability and activity of siRNA by terminal substitution with serinol nucleic acid (SNA", CHEMBIOCHEM, vol. 15, 2014, pages 2549 - 2555, XP055796419
KAMIYA, Y. ET AL.: "Introduction of 2,6-diaminopurines into serinol nucleic acid improves anti-miRNA performance", CHEMBIOCHEM, vol. 18, 2017, pages 1917 - 1922, XP055796558
MURAYAMA, K. ET AL.: "Ultrasensitive molecular beacon designed with totally serinol nucleic acid (SNA) for monitoring mRNA in cells", CHEMBIOCHEM, vol. 16, 2015, pages 1298 - 1301, XP055568346, DOI: 10.1002/cbic.201500167
ASANUMA HIROYUKI: "Functional reinstallation of nucleic acids aiming for medical applications", THE 29TH BANYU SENDAI SYMPOSIUM, ORGANIC SYNTHETIC CHEMISTRY TOWARD THE FUTURE, 1 January 2018 (2018-01-01), pages 1 - 6, XP055904323
KOKURYO, T. ET AL.: "Nek2 siRNA therapy using a portal venous port-catheter system for liver metastasis in pancreatic cancer", CANCER SCIENCE, vol. 107, no. 9, 2016, pages 1315 - 1320, XP055796560
KOKURYO ET AL., CANCER SCI, vol. 107, 2016, pages 1315 - 1320
See also references of EP 4019088A4
KAMIYA, Y. ET AL.: "Introduction of 2,6-diaminopurines into serinol nucleic acid improves anti-miRNA performance", CHEMBIOCHEM, vol. 18, 2017, pages 1917 - 1922, XP055796558
MURAYAMA, K. ET AL.: "Ultrasensitive molecular beacon designed with totally serinol nucleic acid (SNA) for monitoring mRNA in cells", CHEMBIOCHEM, vol. 16, 2015, pages 1298 - 1301, XP055568346, DOI: 10.1002/cbic.201500167
ASANUMA HIROYUKI: "Functional reinstallation of nucleic acids aiming for medical applications", THE 29TH BANYU SENDAI SYMPOSIUM, ORGANIC SYNTHETIC CHEMISTRY TOWARD THE FUTURE, 1 January 2018 (2018-01-01), pages 1 - 6, XP055904323
KOKURYO, T. ET AL.: "Nek2 siRNA therapy using a portal venous port-catheter system for liver metastasis in pancreatic cancer", CANCER SCIENCE, vol. 107, no. 9, 2016, pages 1315 - 1320, XP055796560
KOKURYO ET AL., CANCER SCI, vol. 107, 2016, pages 1315 - 1320
See also references of EP 4019088A4
Attorney, Agent or Firm:
KAI-U PATENT LAW FIRM (JP)
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