Title:
RNA FOR MALARIA VACCINES
Document Type and Number:
WIPO Patent Application WO/2020/128031
Kind Code:
A3
Abstract:
The present invention is directed to a coding RNA for a Malaria vaccine. The coding RNA comprises at least one heterologous untranslated region (UTR), preferably a 3'-UTR and/or a 5'-UTR, and a coding region encoding at least one antigenic peptide or protein derived from a Malaria parasite, in particular at least one antigenic protein derived from circumsporozoite protein (CSP) of a Malaria parasite (e.g. Plasmodium falciparum). The present invention is also directed to compositions and vaccines comprising said coding RNA in association with a polymeric carrier, a polycationic protein or peptide, or a lipid nanoparticle (LNP). Further, the invention concerns a kit, particularly a kit of parts comprising the coding RNA, or the composition, or the vaccine. The invention is also directed to a method of treating or preventing Malaria, and the first and second medical uses of the coding RNA, the composition, the vaccine, and the kit.
Inventors:
SCHWENDT KIM ELLEN (DE)
PETSCH BENJAMIN (DE)
AMBRUSTER NICOLE (DE)
PETSCH BENJAMIN (DE)
AMBRUSTER NICOLE (DE)
Application Number:
PCT/EP2019/086777
Publication Date:
August 13, 2020
Filing Date:
December 20, 2019
Export Citation:
Assignee:
CUREVAC AG (DE)
International Classes:
A61K39/015; A61P33/06
Domestic Patent References:
| WO2018081638A1 | 2018-05-03 | |||
| WO2017070624A1 | 2017-04-27 | |||
| WO2006040334A1 | 2006-04-20 |
Other References:
MONICA CABRERA-MORA ET AL: "Induction of Multifunctional Broadly Reactive T Cell Responses by a Plasmodium vivax Circumsporozoite Protein Recombinant Chimera", INFECTION AND IMMUNITY, vol. 83, no. 9, 13 July 2015 (2015-07-13), US, pages 3749 - 3761, XP055678871, ISSN: 0019-9567, DOI: 10.1128/IAI.00480-15
SAMANEH H. SHABANI ET AL: "Biological, immunological and functional properties of two novel multi-variant chimeric recombinant proteins of CSP antigens for vaccine development against Plasmodium vivax infection", MOLECULAR IMMUNOLOGY., vol. 90, 1 October 2017 (2017-10-01), GB, pages 158 - 171, XP055678901, ISSN: 0161-5890, DOI: 10.1016/j.molimm.2017.06.033
COCKBURN IAN A ET AL: "Malaria prevention: from immunological concepts to effective vaccines and protective antibodies", NATURE IMMUNOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 19, no. 11, 17 October 2018 (2018-10-17), pages 1199 - 1211, XP036617634, ISSN: 1529-2908, [retrieved on 20181017], DOI: 10.1038/S41590-018-0228-6
AMY R. NOE ET AL: "A Full-Length Plasmodium falciparum Recombinant Circumsporozoite Protein Expressed by Pseudomonas fluorescens Platform as a Malaria Vaccine Candidate", PLOS ONE, vol. 9, no. 9, 23 September 2014 (2014-09-23), pages e107764, XP055213153, DOI: 10.1371/journal.pone.0107764
BENJAMIN PETSCH ET AL: "Protective efficacy of in vitro synthesized, specific mRNA vaccines against influenza A virus infection", NATURE BIOTECHNOLOGY, vol. 30, no. 12, 1 January 2012 (2012-01-01), pages 1210 - 1216, XP055051005, ISSN: 1087-0156, DOI: 10.1038/nbt.2436
DANIEL X. JOHANSSON ET AL: "Intradermal Electroporation of Naked Replicon RNA Elicits Strong Immune Responses", PLOS ONE, vol. 7, no. 1, 4 January 2012 (2012-01-04), pages e29732, XP055337400, DOI: 10.1371/journal.pone.0029732
KENNETH LUNDSTROM: "Latest development on RNA-based drugs and vaccines", FUTURE SCIENCE OA, vol. 4, no. 5, 1 June 2018 (2018-06-01), pages FSO300, XP055601504, DOI: 10.4155/fsoa-2017-0151
NORBERT PARDI ET AL: "mRNA vaccines - a new era in vaccinology", NATURE REVIEWS. DRUG DISCOVERY, vol. 17, no. 4, 12 January 2018 (2018-01-12), GB, pages 261 - 279, XP055524319, ISSN: 1474-1776, DOI: 10.1038/nrd.2017.243
LJUNGBERG KARL ET AL: "Self-replicating alphavirus RNA vaccines", EXPERT REVIEW OF VACCINES, EXPERT REVIEWS LTD, GB, vol. 14, no. 2, 1 February 2015 (2015-02-01), pages 177 - 194, XP008175780, ISSN: 1744-8395, DOI: 10.1586/14760584.2015.965690
GUGLIELMO LUCCHESE ET AL: "How a single amino acid change may alter the immunological information of a peptide", FRONTIERS IN BIOSCIENCE : ELITE EDITION, vol. 4, no. 5, 1 January 2012 (2012-01-01), US, pages 1843 - 1852, XP055467091, ISSN: 1945-0494, DOI: 10.2741/e506
TIMOTHY W. PHARES ET AL: "Rhesus macaque and mouse models for down-selecting circumsporozoite protein based malaria vaccines differ significantly in immunogenicity and functional outcomes", MALARIA JOURNAL, vol. 16, no. 1, 13 March 2017 (2017-03-13), XP055711971, DOI: 10.1186/s12936-017-1766-3
SAMANEH H. SHABANI ET AL: "Biological, immunological and functional properties of two novel multi-variant chimeric recombinant proteins of CSP antigens for vaccine development against Plasmodium vivax infection", MOLECULAR IMMUNOLOGY., vol. 90, 1 October 2017 (2017-10-01), GB, pages 158 - 171, XP055678901, ISSN: 0161-5890, DOI: 10.1016/j.molimm.2017.06.033
COCKBURN IAN A ET AL: "Malaria prevention: from immunological concepts to effective vaccines and protective antibodies", NATURE IMMUNOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 19, no. 11, 17 October 2018 (2018-10-17), pages 1199 - 1211, XP036617634, ISSN: 1529-2908, [retrieved on 20181017], DOI: 10.1038/S41590-018-0228-6
AMY R. NOE ET AL: "A Full-Length Plasmodium falciparum Recombinant Circumsporozoite Protein Expressed by Pseudomonas fluorescens Platform as a Malaria Vaccine Candidate", PLOS ONE, vol. 9, no. 9, 23 September 2014 (2014-09-23), pages e107764, XP055213153, DOI: 10.1371/journal.pone.0107764
BENJAMIN PETSCH ET AL: "Protective efficacy of in vitro synthesized, specific mRNA vaccines against influenza A virus infection", NATURE BIOTECHNOLOGY, vol. 30, no. 12, 1 January 2012 (2012-01-01), pages 1210 - 1216, XP055051005, ISSN: 1087-0156, DOI: 10.1038/nbt.2436
DANIEL X. JOHANSSON ET AL: "Intradermal Electroporation of Naked Replicon RNA Elicits Strong Immune Responses", PLOS ONE, vol. 7, no. 1, 4 January 2012 (2012-01-04), pages e29732, XP055337400, DOI: 10.1371/journal.pone.0029732
KENNETH LUNDSTROM: "Latest development on RNA-based drugs and vaccines", FUTURE SCIENCE OA, vol. 4, no. 5, 1 June 2018 (2018-06-01), pages FSO300, XP055601504, DOI: 10.4155/fsoa-2017-0151
NORBERT PARDI ET AL: "mRNA vaccines - a new era in vaccinology", NATURE REVIEWS. DRUG DISCOVERY, vol. 17, no. 4, 12 January 2018 (2018-01-12), GB, pages 261 - 279, XP055524319, ISSN: 1474-1776, DOI: 10.1038/nrd.2017.243
LJUNGBERG KARL ET AL: "Self-replicating alphavirus RNA vaccines", EXPERT REVIEW OF VACCINES, EXPERT REVIEWS LTD, GB, vol. 14, no. 2, 1 February 2015 (2015-02-01), pages 177 - 194, XP008175780, ISSN: 1744-8395, DOI: 10.1586/14760584.2015.965690
GUGLIELMO LUCCHESE ET AL: "How a single amino acid change may alter the immunological information of a peptide", FRONTIERS IN BIOSCIENCE : ELITE EDITION, vol. 4, no. 5, 1 January 2012 (2012-01-01), US, pages 1843 - 1852, XP055467091, ISSN: 1945-0494, DOI: 10.2741/e506
TIMOTHY W. PHARES ET AL: "Rhesus macaque and mouse models for down-selecting circumsporozoite protein based malaria vaccines differ significantly in immunogenicity and functional outcomes", MALARIA JOURNAL, vol. 16, no. 1, 13 March 2017 (2017-03-13), XP055711971, DOI: 10.1186/s12936-017-1766-3
Attorney, Agent or Firm:
VON STOSCH, Andreas et al. (DE)
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