Title:
RNAI-BASED SENSORS, CAGED INTERFERING RNAS, AND METHODS OF USE THEREOF
Document Type and Number:
WIPO Patent Application WO2004045547
Kind Code:
A3
Abstract:
Methods of using labeled interfering RNAs to detect and/or quantitate target mRNAs in cells are provided. Related compositions, systems, and kits are also provided. Caged interfering RNAs (e.g., photoactivatable interfering RNAs), methods of using such caged RNAs, and related systems and kits are also provided. Methods and compositions for introducing interfering RNAs into cells, using RNAs covalently associated with protein transduction domains and/or lipids, are provided. Also provided are methods and compositions for selectively attenuating expression of a target mRNA by controlling expression of an interfering RNA.
Inventors:
NGUYEN QUAN (US)
MCMASTER GARY (US)
MCMASTER GARY (US)
Application Number:
PCT/US2003/036889
Publication Date:
March 31, 2005
Filing Date:
November 17, 2003
Export Citation:
Assignee:
GENOSPECTRA INC (US)
NGUYEN QUAN (US)
MCMASTER GARY (US)
NGUYEN QUAN (US)
MCMASTER GARY (US)
International Classes:
A61K48/00; C07H21/02; C12N15/85; C12Q1/00; C12Q1/37; C12Q1/48; C12Q1/68; G01N33/542; G01N33/573; (IPC1-7): C12Q1/68; C07H21/02; C07H21/04; C12N15/63; C12N15/85; C12N15/87
Foreign References:
| US6017758A | 2000-01-25 |
Other References:
ELBASHIR S.M. ET AL.: "Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells", NATURE, vol. 411, 24 May 2001 (2001-05-24), pages 494 - 498, XP002206451
MCCAFFREY A. ET AL.: "RNA interference in adult mice", NATURE, vol. 418, 4 July 2002 (2002-07-04), pages 38 - 39, XP002234152
SOKOL D. ET AL.: "Real time detection of DNA-RNA hybridization in living cells", PROC. NATL. ACAD. SCI. USA, vol. 95, September 1998 (1998-09-01), USA, pages 11538 - 11543, XP002123471
SIXOU S. ET AL.: "Intracellular oligonucleotide hybridization detected by fluorescence resonance energy transfer (FRET)", NUCL. ACIDS RES., vol. 22, no. 4, February 1994 (1994-02-01), pages 662 - 668, XP002926820
FISCHER P.M. ET AL.: "Cellular delivery of impermeable effector molecules in the form of conjugates with peptides capable of mediating membrane translocation", BIOCONJ. CHEM., vol. 12, no. 6, November 2001 (2001-11-01) - December 2001 (2001-12-01), pages 825 - 841, XP002259060
ANDO H. ET AL.: "Photo-mediated gene activation using caged RNA/DNA in zebrafish embryos", NATURE GENETICS, vol. 28, August 2001 (2001-08-01), pages 318 - 320, XP002203447
MCCAFFREY A. ET AL.: "RNA interference in adult mice", NATURE, vol. 418, 4 July 2002 (2002-07-04), pages 38 - 39, XP002234152
SOKOL D. ET AL.: "Real time detection of DNA-RNA hybridization in living cells", PROC. NATL. ACAD. SCI. USA, vol. 95, September 1998 (1998-09-01), USA, pages 11538 - 11543, XP002123471
SIXOU S. ET AL.: "Intracellular oligonucleotide hybridization detected by fluorescence resonance energy transfer (FRET)", NUCL. ACIDS RES., vol. 22, no. 4, February 1994 (1994-02-01), pages 662 - 668, XP002926820
FISCHER P.M. ET AL.: "Cellular delivery of impermeable effector molecules in the form of conjugates with peptides capable of mediating membrane translocation", BIOCONJ. CHEM., vol. 12, no. 6, November 2001 (2001-11-01) - December 2001 (2001-12-01), pages 825 - 841, XP002259060
ANDO H. ET AL.: "Photo-mediated gene activation using caged RNA/DNA in zebrafish embryos", NATURE GENETICS, vol. 28, August 2001 (2001-08-01), pages 318 - 320, XP002203447
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