Title:
RPA PRIMER COMPOSITION AND METHOD FOR DETECTING SHIGA TOXIN-PRODUCING ESCHERICHIA COLI
Document Type and Number:
WIPO Patent Application WO/2021/218006
Kind Code:
A1
Abstract:
Provided are an RPA primer composition and method for detecting shiga toxin-producing Escherichia coli. The primer composition is used for detecting nine gene subtypes, namely, stx1a, stx1c, stx1d, stx2a, stx2b, stx2c, stx2d, stx2e and stx2g and comprises a primer pair with sequences shown as SEQ ID NO: 1 and SEQ ID NO: 4 and a primer pair with sequences shown as SEQ ID NO: 9 and SEQ ID NO: 12. In combination with a centrifugal disc type micro-fluidic chip technology, rapid and high-throughput detection on STEC in foods, medicines and livestock breeding products can be realized within an extremely small volume (5 μL).
Inventors:
FAN YILING (CN)
WANG SHUJUAN (CN)
LI QIONGQIONG (CN)
SONG MINGHUI (CN)
QIN FENG (CN)
LIU HAO (CN)
YANG MEICHENG (CN)
WANG SHUJUAN (CN)
LI QIONGQIONG (CN)
SONG MINGHUI (CN)
QIN FENG (CN)
LIU HAO (CN)
YANG MEICHENG (CN)
Application Number:
PCT/CN2020/116406
Publication Date:
November 04, 2021
Filing Date:
September 21, 2020
Export Citation:
Assignee:
SHANGHAI INST FOR FOOD AND DRUG CONTROL (CN)
International Classes:
C12Q1/689; C12Q1/10; C12Q1/6844; C12R1/19
Domestic Patent References:
| WO2019178188A1 | 2019-09-19 |
Foreign References:
| CN110452964A | 2019-11-15 | |||
| CN111500753A | 2020-08-07 | |||
| CN108531631A | 2018-09-14 |
Other References:
MATTHEW THOMAS, TIMOTHY JANZEN, NORIKO GOJI, VICTORIA ARLING, BURTON BLAIS, DELE OGUNREMI, AMIT MATHEWS, KINGSLEY AMOAKO: "Rapid detection of Shiga toxin producing Escherichia coli using recombinase polymerase amplification", JOURNAL OF FOOD PROTECTION, vol. 82, no. Suppl. 1, 31 December 2019 (2019-12-31), pages 290, XP009531412, ISSN: 0362-028X, DOI: 10.4315/0362-028X-82.sp1.1
FAN YILING, SHUJUAN WANG, QIONGQIONG LI, YING HU, MINGHUI SONG, FENG QIN, HAO LIU, MEICHENG YANG: "Rapid Detection of Shiga Toxin-producing Escherichia coli by Recombinase Polymerase Amplification Combined with Centrifugal Compact Disc Microfluidic Chip", FOOD SCIENCE, vol. 42, no. 10, 22 July 2020 (2020-07-22), pages 297 - 304, XP055862236
MURINDA SHELTON E., IBEKWE A. MARK, ZULKAFFLY SYAIZUL, CRUZ ANDREW, PARK STANLEY, RAZAK NUR, MD PAUDZAI FARAH, AB SAMAD LIANA, BAQ: "Real-Time Isothermal Detection of Shiga Toxin–Producing Escherichia coli Using Recombinase Polymerase Amplification", FOODBORNE PATHOGENS AND DISEASE, MARY ANN LIEBERT, INC. PUBLISHERS, US, vol. 11, no. 7, 1 July 2014 (2014-07-01), US , pages 529 - 536, XP055862203, ISSN: 1535-3141, DOI: 10.1089/fpd.2013.1663
FAN YILING, SHUJUAN WANG, QIONGQIONG LI, YING HU, MINGHUI SONG, FENG QIN, HAO LIU, MEICHENG YANG: "Rapid Detection of Shiga Toxin-producing Escherichia coli by Recombinase Polymerase Amplification Combined with Centrifugal Compact Disc Microfluidic Chip", FOOD SCIENCE, vol. 42, no. 10, 22 July 2020 (2020-07-22), pages 297 - 304, XP055862236
MURINDA SHELTON E., IBEKWE A. MARK, ZULKAFFLY SYAIZUL, CRUZ ANDREW, PARK STANLEY, RAZAK NUR, MD PAUDZAI FARAH, AB SAMAD LIANA, BAQ: "Real-Time Isothermal Detection of Shiga Toxin–Producing Escherichia coli Using Recombinase Polymerase Amplification", FOODBORNE PATHOGENS AND DISEASE, MARY ANN LIEBERT, INC. PUBLISHERS, US, vol. 11, no. 7, 1 July 2014 (2014-07-01), US , pages 529 - 536, XP055862203, ISSN: 1535-3141, DOI: 10.1089/fpd.2013.1663
Attorney, Agent or Firm:
SHANGHAI SHENXIN LAW FIRM (CN)
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