Title:
SACCHAROMYCES CEREVISIAE STRAIN FOR PRODUCING BREAST MILK LIPID SUBSTITUTE AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/027094
Kind Code:
A1
Abstract:
Provided is a saccharomyces cerevisiae strain for producing a breast milk lipid substitute. By means of integrating a heterologous lysophosphatidic acid acyltransferase into saccharomyces cerevisiae and knocking out a self-natural lysophosphatidic acid acyltransferase, the content of palmitic acid (C16:0) at position Sn-2 in triglyceride produced by the saccharomyces cerevisiae is increased, and the breast milk fat substitute is synthesized; and on this basis, by means of knocking out a metabolic pathway-related gene, the content of the breast milk lipid substitute in the product is further increased. The saccharomyces cerevisiae is used for synthesizing the breast milk lipid substitute from scratch, such that the content of total fatty acids reaches 15% or more, and the relative content of C16:0 at position Sn-2 reaches about 60%.
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Inventors:
LIU LONG (CN)
CHEN JIAN (CN)
LV XUEQIN (CN)
WU WENYANG (CN)
DU GUOCHENG (CN)
LI JIANGHUA (CN)
ZHOU GUOLIN (CN)
LIU YANFENG (CN)
ZHANG CHENYANG (CN)
CHEN JIAN (CN)
LV XUEQIN (CN)
WU WENYANG (CN)
DU GUOCHENG (CN)
LI JIANGHUA (CN)
ZHOU GUOLIN (CN)
LIU YANFENG (CN)
ZHANG CHENYANG (CN)
Application Number:
PCT/CN2022/143077
Publication Date:
February 08, 2024
Filing Date:
December 29, 2022
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
INNER MONGOLIA MENGNIU DAIRY GROUP COMPANY LTD (CN)
INNER MONGOLIA MENGNIU DAIRY GROUP COMPANY LTD (CN)
International Classes:
C12N1/19; A23D9/00; A23D9/04; C12N15/31; C12N15/54; C12N15/55; C12N15/81; C12P7/6463
Foreign References:
| US20170191073A1 | 2017-07-06 | |||
| CN110846293A | 2020-02-28 | |||
| JP2007209240A | 2007-08-23 | |||
| US20110183387A1 | 2011-07-28 | |||
| US20130149754A1 | 2013-06-13 | |||
| US20160145599A1 | 2016-05-26 | |||
| CN108486176A | 2018-09-04 |
Other References:
KIM YEONGHO, TERNG EE LENG, RIEKHOF WAYNE R., CAHOON EDGAR B., CERUTTI HERIBERTO: "Endoplasmic reticulum acyltransferase with prokaryotic substrate preference contributes to triacylglycerol assembly in Chlamydomonas", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 115, no. 7, 13 February 2018 (2018-02-13), pages 1652 - 1657, XP093136258, ISSN: 0027-8424, DOI: 10.1073/pnas.1715922115
Attorney, Agent or Firm:
CENTRAL SOUTH WELL INTELLECTUAL PROPERTY OFFICE (CN)
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