Title:
SCREENING METHOD FOR HETEROLOGOUS COMPETITIVE ANTIGEN FOR USE IN IMPROVEMENT OF IMMUNODETECTION SENSITIVITY
Document Type and Number:
WIPO Patent Application WO/2022/166129
Kind Code:
A1
Abstract:
A screening method for a heterologous competitive antigen for use in improvement of immunodetection sensitivity. The method comprises: performing corresponding molecular descriptor calculation on enrofloxacin analogues, and performing principal component analysis on molecular descriptors to obtain a principal component analysis result (S101); respectively performing measurement to obtain the half maximal inhibitory concentration of enrofloxacin and the half maximal inhibitory concentration of each enrofloxacin analogue, so as to calculate a cross-reactivity rate of each enrofloxacin analogue (S102); according to the principal component analysis result and the cross-reactivity rate of the corresponding enrofloxacin analogue, establishing a mathematical model and performing classification learning to obtain a classification learning result (S103); and according to the classification learning result, performing molecular docking by using a quinolone molecular cross object and lysine, so as to obtain a conformation, and performing configuration optimization to obtain a minimum energy conformation, so as to determine an optimal heterologous competitive antigen (S104). The method facilitates the screening and determining of the heterologous competitive antigen capable of improving immunodetection sensitivity, and has a good application prospect.
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Inventors:
GUO DEBIN (CN)
SU TING (CN)
GUO ZHEN (CN)
PENG JUAN (CN)
MAO CHUNCAI (CN)
LI HUI (CN)
SU TING (CN)
GUO ZHEN (CN)
PENG JUAN (CN)
MAO CHUNCAI (CN)
LI HUI (CN)
Application Number:
PCT/CN2021/108338
Publication Date:
August 11, 2022
Filing Date:
July 26, 2021
Export Citation:
Assignee:
JIANGXI HUANGSHANGHUANG GROUP FOOD CO LTD (CN)
International Classes:
G01N33/577; G06K9/62; G01N33/94; G06N20/00
Foreign References:
| CN113033606A | 2021-06-25 | |||
| CN102708269A | 2012-10-03 | |||
| CN111402967A | 2020-07-10 | |||
| CN110890137A | 2020-03-17 | |||
| US20070156343A1 | 2007-07-05 |
Other References:
HU SONG: "Novel Labels and Heterologous Competitive Systems for Improving Sensitivity of Immunoassay", MASTER THESIS, TIANJIN POLYTECHNIC UNIVERSITY, CN, no. 1, 15 January 2021 (2021-01-15), CN , XP055955441, ISSN: 1674-0246
Attorney, Agent or Firm:
WENZHOU MINGCHUANG INTELLECTUAL PROPERTY AGENCY CO., LTD (CN)
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