Title:
SCREENING PROCESS FOR PLURIPOTENT STEM CELL PROPAGATION PROMOTING FACTOR
Document Type and Number:
WIPO Patent Application WO/2014/208295
Kind Code:
A1
Abstract:
The present invention pertains to a screening method for a propagation promoting factor for pluripotent stem cells, employing a conditioned medium produced by culturing feeder cells in a serum-free medium which contains L-ascorbic acid, insulin, transferrin, selenium, and sodium hydrogen carbonate, but which does not contain serum or a serum replacement; to a pluripotent stem cell propagation method involving feeder free culture using the conditioned medium; and to a pluripotent stem cell propagation method involving feeder free culture of pluripotent stem cells prepared beforehand by on-feeder culture in the serum-free medium.
Inventors:
KATO TOMOHISA (JP)
KANEMURA YONEHIRO (JP)
SHOFUDA TOMOKO (JP)
FUKUSUMI HAYATO (JP)
KANEMURA YONEHIRO (JP)
SHOFUDA TOMOKO (JP)
FUKUSUMI HAYATO (JP)
Application Number:
PCT/JP2014/064764
Publication Date:
December 31, 2014
Filing Date:
June 03, 2014
Export Citation:
Assignee:
KANEKA CORP (JP)
NAT HOSPITAL ORGANIZATION (JP)
NAT HOSPITAL ORGANIZATION (JP)
International Classes:
C12Q1/02; C12N5/0735; C12N5/10
Foreign References:
| JP2013137206A | 2013-07-11 |
Other References:
CHIN A.C.P. ET AL.: "Identification of proteins from feeder conditioned medium that support human embryonic stem cells", J.BIOTECHNOL., vol. 130, no. 3, 2007, pages 320 - 8, XP022119696
CHEN G. ET AL.: "Chemically defined conditions for human iPSC derivation and culture", NAT. METHODS, vol. 8, no. 5, 2011, pages 424 - 9, XP055092759
LIM J.W.E. ET AL.: "Proteome analysis of conditioned medium from mouse embryonic fibroblast feeder layers which support the growth of human embryonic stem cells", PROTEOMICS, vol. 2, no. 9, 2002, pages 1187 - 203, XP008042735
HOVATTA O. ET AL., HUM. REPROD., vol. 18, 2003, pages 1404 - 1409
RICHARDS M. ET AL., NAT. BIOTECHNOL., vol. 20, 2002, pages 933 - 936
CHENG L. ET AL., STEM CELLS, vol. 21, 2003, pages 131 - 142
RICHARDS M ET AL., STEM CELLS, vol. 21, 2003, pages 546 - 556
YUE X-S ET AL., PLOS. ONE, vol. 7, 2012, pages E32707
HAYATO FUKUSUMI; YONEHIRO KANEMURA: "Development of Feeder-free Cell Culture Technique of Human ES/iPS Cells", JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, vol. 239, 2011, pages 1338 - 1344
AKOPIAN V. ET AL., IN VITRO CELL DEV. BIOL. ANIM, vol. 46, 2010, pages 247 - 258
CHEN G ET AL., NAT. METHODS, vol. 8, 2011, pages 424 - 429
CHIN A. C. ET AL., J. BIOTECHNOL., vol. 130, 2007, pages 320 - 328
SHAMBLOTT M. J. ET AL., PROC. NATL. ACAD. SCI., U.S.A., vol. 95, 1998, pages 13726 - 13731
CONRAD S., NATURE, vol. 456, 2008, pages 344 - 349
NATURE, vol. 505, 2014, pages 641 - 647
ZHANG ET AL., NATURE COMMUNICATIONS, vol. 4, 2013, pages 1335
TAKAHASHI K. ET AL., CELL, vol. 131, 2007, pages 1 - 12
D. KANEMATSU ET AL., DIFFERENTIATION, vol. 82, 2011, pages 77 - 88
ZHANG ET AL., NATURE COMMUNICATIONS, vol. 4, 2013
CHEN G. ET AL.: "Chemically defined conditions for human iPSC derivation and culture", NAT. METHODS, vol. 8, no. 5, 2011, pages 424 - 9, XP055092759
LIM J.W.E. ET AL.: "Proteome analysis of conditioned medium from mouse embryonic fibroblast feeder layers which support the growth of human embryonic stem cells", PROTEOMICS, vol. 2, no. 9, 2002, pages 1187 - 203, XP008042735
HOVATTA O. ET AL., HUM. REPROD., vol. 18, 2003, pages 1404 - 1409
RICHARDS M. ET AL., NAT. BIOTECHNOL., vol. 20, 2002, pages 933 - 936
CHENG L. ET AL., STEM CELLS, vol. 21, 2003, pages 131 - 142
RICHARDS M ET AL., STEM CELLS, vol. 21, 2003, pages 546 - 556
YUE X-S ET AL., PLOS. ONE, vol. 7, 2012, pages E32707
HAYATO FUKUSUMI; YONEHIRO KANEMURA: "Development of Feeder-free Cell Culture Technique of Human ES/iPS Cells", JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, vol. 239, 2011, pages 1338 - 1344
AKOPIAN V. ET AL., IN VITRO CELL DEV. BIOL. ANIM, vol. 46, 2010, pages 247 - 258
CHEN G ET AL., NAT. METHODS, vol. 8, 2011, pages 424 - 429
CHIN A. C. ET AL., J. BIOTECHNOL., vol. 130, 2007, pages 320 - 328
SHAMBLOTT M. J. ET AL., PROC. NATL. ACAD. SCI., U.S.A., vol. 95, 1998, pages 13726 - 13731
CONRAD S., NATURE, vol. 456, 2008, pages 344 - 349
NATURE, vol. 505, 2014, pages 641 - 647
ZHANG ET AL., NATURE COMMUNICATIONS, vol. 4, 2013, pages 1335
TAKAHASHI K. ET AL., CELL, vol. 131, 2007, pages 1 - 12
D. KANEMATSU ET AL., DIFFERENTIATION, vol. 82, 2011, pages 77 - 88
ZHANG ET AL., NATURE COMMUNICATIONS, vol. 4, 2013
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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