SELECTIVE FLUORESCENT PROBES FOR MEASURING MULTIDRUG TRANSPORTER ACTIVITY

Title:

SELECTIVE FLUORESCENT PROBES FOR MEASURING MULTIDRUG TRANSPORTER ACTIVITY

Document Type and Number:

WIPO Patent Application WO/2024/028620

Kind Code:

A3

Abstract:

Selective fluorescent probes for measuring multidrug transporter activity The present invention relates to a fluorescent dye accumulation assay for parallel measurements of ABC multidrug transporters, as efflux pump, capable of extruding cyanine dyes. In particular, the invention relates to measurements of the ABCB1 multidrug transporter functions via detecting accumulation of the fluorescent dye in a cell. The invention also includes a diagnostic application of the functional assays in normal and malignant human cells, preferably blood cells.

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Inventors:

SZABÓ EDIT ZSUZSANNA (HU)
VÁRADY GYÖRGY (HU)
SARKADI BALÁZS (HU)
GELENCSÉRNÉ KULIN ANNA (HU)
JEZSÓ BÁLINT (HU)
KUCSMA NÓRA (HU)

Application Number:

PCT/HU2023/050047

Publication Date:

April 04, 2024

Filing Date:

August 01, 2023

Export Citation:

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Assignee:

TERMESZETTUDOMANYI KUTATOKOEZPONT (HU)

International Classes:

G01N33/50; G01N33/58

Other References:

STROUSE J JACOB ET AL: "Fluorescent substrates for flow cytometric evaluation of efflux inhibition in ABCB1, ABCC1, and ABCG2 transporters", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, AMSTERDAM, NL, vol. 437, no. 1, 5 March 2013 (2013-03-05), pages 77 - 87, XP028545924, ISSN: 0003-2697, DOI: 10.1016/J.AB.2013.02.018
SZABÓ EDIT ET AL: "A new fluorescent dye accumulation assay for parallel measurements of the ABCG2, ABCB1 and ABCC1 multidrug transporter functions", PLOS ONE, vol. 13, no. 1, 17 November 2018 (2018-11-17), pages e0190629, XP055824472, DOI: 10.1371/journal.pone.0190629

Attorney, Agent or Firm:

DANUBIA PATENT AND LAW OFFICE LLC (HU)

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