Title:
SENESCENT CELL DETERMINATION SYSTEM
Document Type and Number:
WIPO Patent Application WO/2022/071459
Kind Code:
A1
Abstract:
Provided is a means for non-destructively and rapidly determining the senescence level of cultured target cells such as myoblasts. During a centrifugation treatment, the shape of cells, said cells having been separated from the surface of a culture substrate, returns from a flat and spread out shape to a round shape and a cell having a higher senescence level takes a longer time to return to the round shape. By using this phenomenon, the system of the present invention determines the senescence level of a cell by detecting the returning speed of the cell on the basis of the luminance, reflectance or image processing data. The optimum value of the centrifugation parameter is improved by learning, which results in an increase in the senescence level determination accuracy.
More Like This:
Inventors:
NOGUCHI ERI (JP)
MATSUMURA MASAKI (JP)
MATSUMURA MASAKI (JP)
Application Number:
PCT/JP2021/036036
Publication Date:
April 07, 2022
Filing Date:
September 30, 2021
Export Citation:
Assignee:
TERUMO CORP (JP)
International Classes:
C12M1/34; C12Q1/04
Domestic Patent References:
| WO2020045652A1 | 2020-03-05 | |||
| WO2018012601A1 | 2018-01-18 |
Foreign References:
| JP2016523254A | 2016-08-08 |
Other References:
IWANAGA KOICHI, MURATA TAKAHISA, HORI MASATOSHI, OZAKI HIROSHI: "Isolation and Characterization of Bovine Intestinal Subepithelial Myofibroblasts", JOURNAL OF PHARMACOLOGICAL SCIENCES, vol. 112, no. 1, 1 January 2010 (2010-01-01), JP , pages 98 - 104, XP055917624, ISSN: 1347-8613, DOI: 10.1254/jphs.09258FP
ANONYMOUS: "Cells of the Aged", J-STAGE, vol. 15, no. 2, 1 January 1978 (1978-01-01), pages 106 - 107, XP055917635
ANONYMOUS: "Cells of the Aged", J-STAGE, vol. 15, no. 2, 1 January 1978 (1978-01-01), pages 106 - 107, XP055917635
Attorney, Agent or Firm:
KUZUWA, Kiyoshi (JP)
Download PDF:
Previous Patent: SENESCENT CELL DETERMINATION SYSTEM
Next Patent: AIR-CONDITIONING AND VENTILATION SYSTEM
Next Patent: AIR-CONDITIONING AND VENTILATION SYSTEM