SEPARATION METHOD OF FAT AND LEAN USING ACIDIC FLUID WITH

NANOBUBBLES

SUMMARY

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material such that the fat is rigidly frozen and is friable but lean meat and remains flexible; reducing the lean meat-containing material into particles, wherein the particles include particles that have a majority of lean meat and generally smaller particles that have a majority of fat; combining the particles with a fluid, wherein the fluid includes nanobubbles, and the fluid includes water and one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, halogen, chlorine, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide; generating the nanobubbles in a tower having semispherical baffles arranged along a length of the tower; and collecting particles that float in the fluid or collecting particles that sink in the fluid. In an embodiment, the method further comprises transferring a majority of the fluid with the particles that were not collected and separating the majority of the fluid.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material so as to rigidly freeze the fat while the lean meat remains flexible; reducing the lean meat-containing material into particles, wherein the particles include particles that have a majority of lean meat and particles that have a majority of fat; generating gas nanobubbles in a fluid by passing the fluid through a tower having semispherical baffles arranged along a length of the tower; combining the particles with the fluid containing the gas nanobubbles; and collecting particles that float in the fluid or collecting particles that sink in the fluid. In an embodiment, the method further comprises transferring a majority of the fluid with the particles that were not collected and separating the majority of the fluid. In an embodiment, a method for separating fat particles from lean particles, comprises providing beef pieces, wherein the beef pieces comprise fat and lean; lowering the temperature of the diced beef pieces, wherein the fat is reduced to a first temperature at which the fat is rigid and friable while simultaneously achieving a second condition for the lean at which the lean is less rigid and substantially flexible; crushing the beef pieces to liberate the fat into small separated particles without substantially fracturing lean and creating fat particles and lean particles; generating gas nanobubbles in a fluid by passing the fluid through a tower having semispherical baffles arranged along a length of the tower; combining the fat particles and the lean particles with the fluid containing gas nanobubbles to provide a mixture; and collecting particles that float in the fluid or collecting particles that sink in the fluid. In an embodiment, the method further comprises transferring a majority of the fluid with the particles that were not collected and separating the majority of the fluid.

In an embodiment, a method for separating lean meat from lean meat-containing material, comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material while avoiding completely freezing the lean meat; such that the fat is rigidly frozen and is friable, but lean meat is not frozen rigidly and remains substantially flexible when transferred between crushing rollers; reducing the lean meat-containing material into particles, wherein the particles include particles that have a majority of lean meat and particles that have a majority of fat; combining the particles with a fluid, wherein the fluid includes water and one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, halogen, chlorine, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide; transferring the fluid and particles through an elongated vessel aligned horizontally; collecting particles that float in the fluid from the top of the vessel; continuing to transfer a majority of the fluid with the particles that were not collected; and separating the majority of the fluid.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material such that the fat becomes rigidly frozen while the lean meat remains flexible and does not shatter when subjected to a crushing force; reducing the lean meat-containing material into particles, wherein the particles include particles that have a majority of lean meat and particles that have a majority of fat; combining the particles with a fluid; transferring the fluid and particles through an elongated vessel aligned horizontally; collecting particles that float in the fluid from the top of the vessel; continuing to transfer a majority of the fluid with the particles that were not collected; and separating the majority of the fluid.

In an embodiment, a method for separating fat particles from lean particles comprises providing beef pieces, wherein the beef pieces comprise fat and lean; lowering the temperature of the beef pieces, wherein the fat is reduced to a first temperature at which the fat is friable while simultaneously achieving a second temperature for the lean at which the lean is flexible; crushing the beef pieces to liberate the fat without fracturing lean and creating fat particles and lean particles; combining the fat particles and the lean particles with a fluid to provide a mixture; transferring the mixture through an elongated vessel aligned horizontally; collecting particles that float in the fluid from the top of the vessel; continuing to transfer a majority of the fluid with the particles that were not collected; and separating the majority of the fluid.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material while avoiding completely freezing the lean meat; while the fat is rigidly frozen, is friable, and fractures when subjected to a crushing force, but lean meat remains flexible and is not substantially size reduced when subjected to the same crushing force as the fat; reducing the lean meat-containing material into particles, wherein the particles include particles that have a majority of lean meat and smaller particles that have a majority of fat; combining the particles with a fluid in a vortex vessel, wherein the fluid includes water and one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, halogen, chlorine, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide; discharging the fluid and particles from the vortex vessel into a conduit, wherein the conduit is connected to an outlet of the vortex vessel; controlling a level of fluid in the conduit to prevent the introduction of air; transferring the fluid and particles through an elongated separation vessel aligned horizontally which may have slightly upward path so that any air in the elongated separation vessel will move in a direction away from the vortex; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material while such that the fat becomes rigid and the lean meat is flexible; reducing the lean meat-containing material into particles, wherein the particles include particles that have a majority of lean meat and smaller particles that have a majority of fat; combining the particles with a fluid in a vortex vessel; discharging the fluid and particles from the vortex vessel into a conduit, wherein the conduit is connected to an outlet of the vortex vessel; controlling a level of fluid in the conduit to prevent the introduction of air; transferring the fluid and particles through an elongated separation vessel aligned horizontally; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for separating fat particles from lean particles comprises providing beef pieces, wherein the beef pieces comprise fat and lean; lowering the temperature of the diced beef pieces, wherein the fat is reduced to a first temperature at which the fat is friable while simultaneously achieving a second temperature for the lean at which the lean is flexible; crushing the beef pieces to liberate the fat without fracturing lean and creating fat particles and lean particles; combining the fat particles and the lean particles with a fluid in a vortex vessel to provide a mixture; discharging the mixture from the vortex vessel into a conduit, wherein the conduit is connected to an outlet of the vortex vessel; controlling the level of fluid in the conduit to prevent the introduction of air; transferring the fluid and particles through an elongated separation vessel aligned horizontally; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material while avoiding completely freezing the lean meat; while the fat is rigidly frozen and is friable but lean meat remains flexible; crushing the chilled lean meat-containing material between a first and second roller to produce particles that have a majority of lean meat and particles that have a majority of fat, wherein the first and second rollers have teeth on a periphery, wherein the teeth have a repeating curving wave pattern; combining the particles with a fluid, wherein the fluid includes water and one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, halogen, chlorine, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide; transferring the fluid and particles through an elongated separation vessel aligned horizontally; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material while such that the fat becomes rigid and friable but the lean meat remains flexible; crushing the chilled lean meat-containing material between a first and second roller to produce particles that have a majority of lean meat and particles that have a majority of fat, wherein the lean particles are larger than the fat particles and the first and second rollers have teeth on a periphery, wherein the teeth have a repeating curving wave pattern; combining the particles with a fluid; transferring the fluid and particles through an elongated separation vessel aligned horizontally; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for separating fat particles from lean particles comprises providing beef pieces, wherein the beef pieces comprise fat and lean and are size reduced; lowering the temperature of the beef pieces, wherein the fat is reduced to a first temperature at which the fat is friable while simultaneously achieving a second temperature for the lean at which the lean is flexible; crushing the chilled beef pieces between a first and second roller to liberate the fat without fracturing lean and creating fat particles and lean particles, wherein the first and second rollers have teeth on a periphery, wherein the teeth have a repeating curving wave pattern; combining the fat particles and the lean particles with a fluid to provide a mixture; transferring the mixture through an elongated separation vessel aligned horizontally; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material while avoiding completely freezing the lean meat; while the fat is rigidly frozen and is friable but lean meat is not frozen rigidly and remains flexible; reducing the chilled lean meat-containing material into particles that have a majority of lean meat and particles that have a majority of fat; preparing a makeup fluid comprising water by adjusting pH from 4.0 to 5.5, by mixing the fluid with a measured quantity of carbon dioxide gas, then transferring the fluid through a conduit within which cavitation is provided to create nanobubbles in the fluid, and adding chlorine to a level of 3 ppm to 50 ppm; combining the particles with the fluid, wherein the fluid includes water and one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, halogen, chlorine, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide; transferring the fluid and particles through an elongated separation vessel aligned horizontally; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material so that the fat becomes rigid and friable while the lean meat is flexible; reducing the chilled lean meat-containing material into particles that have a majority of lean meat and particles that have a majority of fat; preparing a make-up fluid comprising water by adjusting pH from 4.0 to 5.5, adding nanobubbles, and adding chlorine to a level of 3 ppm to 50 ppm; combining the particles with the fluid; transferring the fluid and particles through an elongated separation vessel aligned horizontally; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for separating fat particles from lean particles comprises providing beef pieces, wherein the beef pieces comprise fat and lean; lowering the temperature of the diced beef pieces, wherein the fat is reduced to a first temperature at which the fat is friable while simultaneously achieving a second temperature for the lean at which the lean is flexible; crushing the chilled beef pieces to liberate the fat without fracturing lean and creating smaller fat particles and lean particles that are larger than the fat particles; preparing a make-up fluid comprising water by adjusting pH from 4.0 to 5.5, creating nanobubbles in the fluid, and adding chlorine to a level of 3 ppm to 50 ppm; combining the fat particles and the lean particles with the fluid to provide a mixture; transferring the mixture through an elongated separation vessel aligned horizontally; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for reducing pathogen populations such as E. Coli

0157:H7 that may be present on the surface of meat pieces comprises providing meat pieces comprising lean meat and fat; chilling the meat pieces; preparing a make-up fluid comprising water by adjusting pH from 4.0 to 5.5, by mixing the fluid with a measured quantity of carbon dioxide gas then transferring the fluid through a sealed, modified conduit at such a rate and pressure causing cavitation to create nanobubbles in the fluid, and adding chlorine to a level of 3 ppm to 50 ppm; immersing the meat pieces in the make-up fluid with gentle agitation to ensure all meat piece surfaces are exposed to the fluid, wherein the make-up fluid includes water and one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, halogen, chlorine, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide; removing the meat pieces from the make-up fluid in a manner that results in no more than 0.5% added water to the meat pieces.

In an embodiment, a method for reducing pathogen populations such as E. Coli 0157:H7; other STEC's (Shiga toxin-producing E. Coli) and salmonella that may be present on the surface of beef carcasses following animal slaughter, prior to chilling and carcass disassembly; the method comprising providing freshly slaughtered beef carcasses suspended from a meat rail; providing a cabinet arranged to open and enclose around a suspended beef carcass; providing a series of fluid jets arranged around the inner walls of the cabinet and pointing inward; preparing a make-up fluid comprising water by adjusting pH from 4.0 to 5.5, by mixing the fluid with a measured quantity of carbon dioxide gas then transferring the fluid through a sealed, modified conduit at such a rate and pressure to cause cavitation and thereby generate nanobubbles in the fluid, and adding chlorine to a level of 3 ppm to 50 ppm; enclosing each carcass in the cabinet while still suspended from a meat rail; processing the carcass by transferring the make-up fluid under elevated pressure through the jets arranged inside the cabinet to direct the pressurized fluid onto the surface of the carcass, wherein the pressure of the fluid is sufficient to remove fecal matter, micro-organisms and all undesirable matter from the carcass surface, wherein the make-up fluid includes water and one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, halogen, chlorine, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide; following thorough processing within the cabinet, opening the cabinet to allow removal of the carcass and transfer of the carcass to a chiller; disposing of the fluid or alternatively collecting the fluid after use then recycling the reclaimed fluid after removing all solids and pasteurizing the fluid by firstly elevating the fluid temperature to greater than 160° F followed by chilling the fluid to a temperature below 160°F prior to reuse in the make-up fluid or pressurizing the fluid to a pressure greater than 80,000 psi.

DESCRIPTION OF THE DRAWINGS

The foregoing aspects and many of the attendant advantages of this invention will become more readily appreciated as the same become better understood by reference to the following detailed description, when taken in conjunction with the accompanying drawings, wherein:

FIGURE 1 is a diagrammatical illustration of a chilled beef rapid separation system;

FIGURE 2 is a diagrammatical illustration of centrifuge;

FIGURE 3 is a diagrammatical illustration of settling vessel; FIGURE 4 is a flow diagram of a method for separating lean from fat;

FIGURE 5 is a diagrammatical illustration of vessel for creating nanobubbles; FIGURE 6 is a diagrammatical illustration of a cross-section of the vessel of FIGURE 5;

FIGURE 7 is a diagrammatical illustration of a separation manifold and system;

FIGURE 8 is a diagrammatical illustration of a fat crusher;

FIGURE 9 is a diagrammatical illustration of the surfaces of the fat crusher of FIGURE 8;

FIGURE 10 is a flow diagram of a method and system for separating lean from fat; FIGURE 11 is a diagrammatical illustration of a volute of a sinusoidal pump;

FIGURE 12 is a diagrammatical illustration of a gravity flow screen separator; and

FIGURE 13 is a diagrammatical illustration of a cabinet for rinsing carcasses.

DETAILED DESCRIPTION

Referring now to FIGURE 1 a diagrammatic representation in a cross sectional format is shown of a system known as a Chilled Beef Rapid Separation System (CBRSS) which is arranged to enable the separation of beef components comprising lean beef, connective tissue and beef fat.

A cryogenic freezing tunnel 4 is arranged to reduce the temperature of a continuous stream of size-reduced beef particles 3 having a size of not more than about 1 inch across. The particles 3 contain both lean beef and beef fat in varying proportions. The particles 3 are cut from larger pieces of beef, such as primals, or can be the leftovers or trimmings after the harvesting the primal cuts of beef. The particles 3 are transferred in the direction shown by arrow 2 through space 6 on a conveyor 8 having a controlled speed. Preferably, liquid nitrogen can be provided within space 6 so as to make direct contact with the beef particles 3 in such a manner so as to consistently reduce the temperature of the beef particles 3 to a controlled value.

The speed of conveyor 8 and the quantity of liquid nitrogen provided into space 6 is controlled such that the finished temperature of the beef particles is as follows. After transfer through the temperature-reducing cryogenic tunnel 4, the temperature of lean beef is about 10°F to26°F and is significantly higher than the temperature of the beef fat which is about -5°F to 2°F. The difference in temperature is believe to arise from the differences in the heat transfer coefficient between the two materials. In this way, the beef fat will crumble while the lean remains flexible when subjected to a crushing force. In some embodiments of the cooling step, freezing of the lean completely is avoided, and the fat is preferentially, rigidly frozen and is friable, but lean meat is not frozen rigidly and remains flexible. In some embodiments of the cooling step, the lean meat-containing material is chilled while avoiding freezing the core or center of the lean meat component; while the surface of the lean meat is non-frozen. In some embodiments of the cooling step, the temperature of the diced beef pieces is lowered to a first reduced temperature for the fat at which the fat is friable while simultaneously achieving a second reduced temperature for the lean at which the lean is not frozen solid thereby remaining partly flexible.

The stream of beef particles 3 drops in the form of a "waterfall" in the direction shown by arrow 10 vertically downward and directly into a gap 9 arranged between two steel rollers 22 and 20. Steel cylindrical rollers 22 and 20 are arranged parallel and at the same level having a common centerline 19 and rotating in opposing directions shown by arrows 16 and 18. Roller 20 rotates counterclockwise in the figure, and roller 22 rotates clockwise in the figure. The surface speed of the rollers 22 and 20 is greater than the velocity of the particles which are gathering speed as they fall downward and into the gap 9 between the rollers 22 and 20. The gap can be between about 1/32" to 1/3" wide, but most preferably 1/16" wide. In one embodiment, the shape of the rollers 20 and 22 is shown in FIGURES 8 and 9. FIGURE 8 shows a crushing device 800 with rollers 802 and 804. The rollers 802 and 804 have intermeshing teeth on the outer perimeter, but, still leave a gap 806 between the rollers 802 and 804 at the point of the closest approach. That is, one roller is not driving the other roller through contact of the teeth. The teeth can be arranged in a straight line or in a helical along the length of each roller. In FIGURE 9, a close up of the rollers' 802 and 804 teeth are shown if the surface of the rollers is made straight. A repeating curving wave pattern (with no sharp edges) is shown for both teeth of roller 802 and 804. The roller 802 has teeth of radius Rl and the roller 804 has teeth of radius R2. In an embodiment, radius Rl is the same as radius R2. In an embodiment, radius Rl is not the same as radius R2. Also shown more clearly in FIGURE 9 is the gap 806 showing that the teeth do not make contact.

Returning to FIGURE 1, in some embodiments, the crumbling of the beef pieces will result in particles that comprise predominantly fat, and the leftover pieces from which the fat has been broken off comprise predominantly lean. In some embodiments, the rollers crush the beef pieces to liberate the fat without fracturing the lean thereby creating fat particles and lean particles.

Significant heat is instantly generated within and at the core of the beef particles by friction resulting from the crushing force applied to the beef particles by the steel rollers 22 and 20 such that the average temperature of the processed beef particles is significantly higher than it is prior to crushing. The average beef particle temperature may be in the order of 20°F after passing between rollers 22 and 20, or in the range of 10 °F to 30 °F, or 15 °F to 25 °F.

Directly below the pair of steel rollers 22 and 20 is a cone shaped vessel 24 arranged with a large open top close to the underside of the rollers 22 and 20. The purpose of the vessel 24 is to combine the fat and lean particles with a fluid. In some embodiments, the density of the fluid is greater than the density of a majority of the fat particles and lower than the density of a majority of the lean particles. In this manner, the mostly fat particles will rise or float in the fluid and the mostly lean particles will sink or settle in the fluid. The density of the fluid can be controlled by temperature, by combining with other agents, such as carbon dioxide, or by microbiocidal agents. In some embodiments, the fluid can include water with one or more microbiocidal agents. In some embodiments, the microbiocidal agents can include one or more of hypochlorous acid, hydrochloric acid, bromine, fluorine, any halogen, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide. In some embodiments, any of the foregoing microbiocidal compounds can optionally be combined with water. The cone shaped vessel has inclined interior walls which taper down to a smaller diameter tube section 17. This configuration thereby provides a "vortex" vessel 24, into which a stream of fluid is transferred via pipe 5 in the direction shown by arrow 7. Such stream of fluid can be introduced at a tangent to the interior of the vessel 24. An interior space 26 is therefore defined and provided by the walls of the "vortex" vessel 24 such that size reduced beef particles are directed into the open "vortex'Vvessel top above space 26 and into space 26. The pipe 5 is arranged tangentially relative to the circular wall of vessel 24 terminating at an opening which enters space 26 and therefore forming a volute through which pressurized fluid is transferred. The stream of fluid entering space 26 is provided therein at such a mass flow and velocity so as to cause the stream to follow close to the inner vortex walls, spinning there around and gradually descending toward the pipe section 17 at the lower end of the vortex 24. The fluid stream descends, gradually gathering speed as the vortex narrows toward pipe section 17. In this way, the stream of fat and lean particles is directed into the vortex thereby rapidly mixing with the fluid.

The fluid transferred into the vortex space 26 via pipe 5 in the direction shown by arrow 7 comprises either water or an aqueous alkaline solution or an aqueous acidic solution but most preferably will have been treated to contain nanobubbles of air, oxygen, chlorine, chlorine dioxide or carbon dioxide and having a paramagnetic quality and a particle size of between 50nm to lOOnm. In some embodiments, nanobubbles will be smaller than 50nm. In some embodiments, nanobubbles will be larger than lOOnm. In the size range of about 50nm to lOOnm, the nanobubbles will not immediately rise to the surface, but, instead will drift in the fluid for extended periods of time. In some embodiments, a nanobubble size of about 40nm to about 300nm can be stable and can last in the range of days to months. Fluids with nanobubbles and agents have greater pathogen deactivation qualities, and when optimally applied can reduce any pathogens attached to the beef particles to undetectable populations. The effect of "nanobubbles" made with a paramagnetic gas, such as 0 ₂ , is to reduce surface tension which in turn substantially improves the efficacy of the sanitation materials dissolved in the water (e.g. C10 ₂ - which is paramagnetic but decomposes easily, which itself is 10 times more soluble than Cl ₂ ). Thus, with only the normal quantities of chlorine as is typically dissolved in tap/drinking water, when the water is a nanobubble (air) suspension. Other agents may be used, such as hypochlorous acid, carbonic acid or any other suitable microbiocidal agent as either listed in this disclosure or elsewhere. A nanobubble solution of carbonic acid at a pH of 4 may be effective so as to avoid the need for high pressure and lower pH. The paramagnetic effect of the atmospheric oxygen in nanobubbles (made with air) is believed to be responsible for reducing surface tension of the water in which the nanobubbles of air are suspended. In the case of ozone (0 ₃ ), because 0 ₃ is a diamagnetic and not a paramagnetic gas, there would appear to be no benefit using ozone in the nanobubbles but if dissolved in the water, the efficacy of ozone should be significantly improved. Thus, the level of dissolved ozone could be reduced to a low level as to avoid the issue of rancidity while having adequate pathogen deactivation effect. Also, water with suspended nanobubbles made from air may destroy biofilm and inhibit or prevent biofilm formation, for example by pathogens. FIGURES 5 and 6 show an embodiment of a vessel to create nanobubbles. FIGURE 5 shows a vessel 600 with a top inlet and a bottom inlet. The vessel includes internal partial baffles 602 and 604. The baffles 602 and 604 can be described as semispherical or semicircular meaning they comprise a greater part of a sphere. In one embodiment, the baffles 602 are placed directly opposite of baffles 604. FIGURE 6 shows an embodiment of a baffle 604 that is semicircular in shape, but, has a segment 606 missing that is defined by the chord line extending between two points on the circumference of the vessel. The baffle 604 generally encompasses a majority of the area, so the chord line defining the missing segment generally will not pass through the center 608. In an embodiment, the baffles 602 and 604 are placed directly opposite so the missing segments are directly opposite from baffle to baffle. However, in other embodiments, the missing segments can be arranged in a helical pattern as baffles are placed along the length of the vessel. The diameter and length of the vessel will be determined by the amount of flow rate desired. The pressure of the fluid at the entrance should be high enough to create cavitation that leads to nanobubble creation. The amount and size of nanobubbles can be measured by taking samples at the outlet of vessel 600. When the amount of the nanobubbles needs to be increased, the inlet pressure to the vessel 600 can be increased to create more cavitation.

The mass flow of beef comprising the stream of beef particles can be transferred at any suitable flow rate but preferably at a rate of about 16,0001bs per hour and the volume of fluid may be transferred via volute/pipe 5 would correspondingly be in the order of 300 to 400 gallons per minute (gpm). The ratio of beef solids, including fat and lean, to fluid is therefore on the order of lib of beef particles to about 81bs to 121bs of fluid or more. It has been demonstrated that if insufficient fluid is provided, separation cannot be readily achieved and a ratio of at least 1 :8 beef versus fluid can provide efficient separation. In some embodiments, in addition to adding turbulence in the vessel to expose surfaces of meat to the microbiocidal fluid, the amount of fluid is also calculated to supply an amount of water that is lost during processing to result in a predetermined proportion of water in the meat. As water can be lost during the cooling step, the addition of the fluid can replenish the water that is lost through evaporation. This will allow packaging the meat containing a predetermined proportion of water in a container. In other embodiments, the amount of water that is to be removed in a centrifuge can be calculated and controlled.

The temperature of the fluid when introduced into vortex vessel 24 is preferably above 34°F and even greater than 40°F while the average temperature of the beef particles can be below 32°F and when combined such that the resultant average temperature of the combined beef particles and fluid is on the order of 37°F to 40°F.

In order to prevent the vortex 24 from overflowing due to an accumulation of too much fluid and beef particles or alternatively allow air to be transferred into pipe 30 due to an inadequate accumulation of fluid and beef solids in the vortex 24, the following arrangement can be provided. A sinusoidal ("sine") pump 28 assembly, including the sine pump drive motor and any integrated gearbox with the vortex 24 (complete sine pump assembly) and pipe connection 17 may be most preferably mounted on load-cells with flexible connections between the pump 28 to pipe 30 and inlet pipe 5 to the vortex 24. The complete sine pump assembly is mounted on load cells in such a way to enable and make available a value representing the weight or mass of the complete pump assembly including the accumulated fluid with solids in the complete assembly at any time during operation, continuously. In this way, the weight value can be used to control the sine pump 28 speed. For example, if the accumulation of fluid and beef solids (the vortex 24 accumulated level) is tending to overfill the vortex 24, the speed and corresponding sine pump mass flow can be correspondingly elevated such that the level of accumulated fluid and solids vortex 24 will be lowered a controlled amount. Alternatively, if the transfer of fluids and solids into the vortex 24 is inadequate such that the accumulated level drops, the sine pump 28 speed can be reduced so as to allow a greater accumulation of fluids and solids in the vortex 24. Accordingly, the overfilling or under-filling of the vortex can be monitored continuously so as to prevent overflow or the transfer of air/gas into pipe 30.

A large sine pump 28 is located directly below pipe section 17 and connected thereto so that the mixture or suspension of beef particles and fluid can be pumped at elevated pressure directly into enclosed pipe section 30 in the direction shown by arrow 11. The mass flow of beef particles and fluid transferred via pump 28 must not be excessive. The supply of beef solids and fluid (suspension) transferred into pipe section 17 must not be less than the amount pumped therefrom via sine pump 28 so as to avoid the transfer of any gas or air (other than the air of gas contained in a nanobubble condition as described above) with the fluid suspension into pipe section 30. FIGURE 11 shows an embodiment of a sine pump arrangement to prevent air or gas or both from entering the sine pump28 and separation manifold 38. The vortex vessel 1036 is connected to the sine pump 1032 via a vertical pipe 1010. The vertical pipe 1010 includes a transparent pipe section 1018 to act as a sight glass which will allow manual speed control of the sine pump 1032 to adjust the level 1034 of the fluid and beef particle in the pipe above the sine pump 1032 and below the vortex vessel 1036. The sine pump 1032 rests on one or more load cells 1030 which enables sine pump speed adjustment to maintain fluid level with the pipe 1014 to prevent air from entering the sine pump and the separation manifold. The vortex vessel 1036 is connected to a fresh fluid inlet pipe 1004 via a flexible pipe section 1006 to allow unrestricted "floating" of the sine pump on the load cell. Similarly, the outlet pipel024 at the discharge of the sine pump includes a flexible pipe section 1022. Thus, the level 1034 in the inlet pipe 1014 substantially prevent any air from entering the sine pump and the separation manifold downstream. A sufficient level 1034 is maintained by weight measurement using the load cell 1030. Alternatively, the sine pump speed can be manually adjusted by visually checking the level in the sight glass 1018.

The suspension is then transferred into vertical pipe section 34 via space 32 in the direction shown by arrow 13. Additional temperature controlled fluid which preferably contains nanobubbles of air, chlorine or a chlorine compound (such as chlorine or chlorine dioxide) or carbon dioxide can be transferred via pipe section 35 in the direction shown by arrow 36 and/or fluid of any temperature controlled selection can be transferred via pipe 14 in the direction shown by arrow 12. The mixture of beef solids and selected fluid are then transferred with any optionally added fluids into horizontal separation manifold section 38.

In some embodiments, the manifold 38 or separator is used in separating particles at different elevations, wherein the particles having a density greater than the fluid will collect at a lower elevation, and the particles that have a density less than the fluid will collect at a relatively higher elevation. In some embodiments, the suspended beef solids and fluid will stratify according to their density while flowing along the horizontal manifold 38 wherein the smaller beef fat particles flow in the direction shown by arrow 37 and in close proximity to the upper, inner surface 40 of the manifold 38 while the larger lean particles sink and flow in the direction shown by arrow 37 along the lower, inner surface 48 of conduit 38. Other particles which may be relatively very few when expressed as a proportion of the solids in the fluid and that comprise a combination of partly beef fat and/or lean and/or connective tissue may remain suspended in the liquid and flowing in the direction of arrow 37 along the central region of the manifold 38 between the upper and lower inner surfaces of the manifold 38.

A first lower port 41 or outlet is located at the conjunction of the underside of manifold 38 and pipe 33 is conveniently located to facilitate the extraction of lean beef particles with a minimized quantity of fluid via pipe 33. A pump 42, which is most preferably a sine pump, is connected directly to the lower end of pipe 33 so as to enable the mass flow controlled extraction of fluid with suspended lean beef particles through pipe 33 in the direction shown by 46 and to then transfer the mixture into pipe sections 44 and 50 and into decanter centrifuge 66 which is described more fully in connection with FIGURE 2. A second lower port 43 or outlet located at the conjunction of manifold 38 and pipe section 57 is arranged to facilitate extraction of limited fluid and lean beef particles in the direction shown by arrow 54 by way of mass flow controlling pump 52 which most preferably is a sine pump. The fluid and solids extracted via pipe 33 and the fluid and solids extracted via pipe 57 are thereby combined, in this configuration at the confluence of pipes 50 and 56. However, the number of lower outlet ports is not limited to what is shown in the figure, and can include one to more than one. The combined quantity of fluid and solids is then transferred in pipe 60 in the direction shown by arrow 58 into decanter centrifuge 66. Optionally, a continuous stream of any selected fluid can be added to the stream of materials transferred in the direction shown by arrow 58 by transfer through pipe 57 in the direction shown by arrow 61.

The combined stream of materials transferred into centrifuge 66 via pipe 60 is then treated generally according to the treatment as described in association with FIGURE 2 wherein a combined stream of fluid and some suspended solids are separated, transferred into pipe 62 in the direction shown by arrow 64 and onto further processing (not shown) or into storage vessels (not shown), whereas lean beef particles are separated from the fluid and transferred via a conduit represented by member 72 in the direction shown by arrow 74 and then onto a conveyor to further processing or packaging. Substantially all lean beef (as in the red muscle content) is separated via the first and second lower outlet ports 41 and 43 such that beef fat particles including connective tissue and the remaining fluid is preferably transferred along the full length of manifold 38 and then upwardly via pipe section 76 and downwardly via pipe section 80 in the direction shown by arrow 82. Optionally a pump 99 is connected to the lower end of pipe 80 wherein the pump 99 is preferably a sine pump which can be used to create back pressure in the space 32 and along the full length of manifold 38 and pipes 76 and 80. A flow regulator 75 can be optionally located in a pipe 78 with an open end 77 so as to provide a means of controlling pressure in the separation manifold 38.

Most preferably the "separation time" for the separated lean beef stream 3 from the fluid is minimized. The "separation time" is the period of time between the instant of combining the beef solids 3 together with the fluid stream transferred via pipe 5 in the direction of arrow 7, together in space 26 and separation of the lean beef particles stream transferred via pipe 72 in the direction shown by arrow 74. The "separation time" period should be not more than 3 minutes but preferably less than 90 seconds, however the "separation time" period may be less than 30 minutes, or less than 20 minutes or less than 5 minutes. The minimized period of "separation time" can ensure that no beef micro- nutrients are separated or removed from the lean beef particles which may otherwise occur.

In an alternative embodiment, two (or more) centrifuges may be employed in place of centrifuge 66 (as described above), wherein pipe 44 is connected directly to a first decanter centrifuge and pipe 56 is connected directly to a second centrifuge. In this way, the first stream of lean beef extracted via port 41 comprises a greater proportion of red muscle lean beef with a lesser proportion of connective tissue than lean beef extracted via port 43 which comprises a lesser proportion of red muscle lean beef with a greater proportion of connective tissue.

In place of a single vortex vessel 24 as disclosed in connection with FIGURE 1, multiple vortex vessels can be arranged whereby the single stream of size reduced beef particles can be divided into a series of streams wherein one stream per vortex vessel is created, with a single set of downstream separation equipment connected to each vortex vessel.

After separation of lean beef and fluid via ports 41 and 43, the remaining stream of matter flowing along manifold 38 comprises a mixture of beef fat, connective tissue and fluid. The remaining stream is then transferred from the manifold via an upper outlet 76 to a flotation tank (separator) via the open end of a conduit 83 in the direction shown by arrow 84. The flotation tank (FIGURE 3) enables separation of the beef fat which is then transferred by way of a suitable pump (preferably a Moyno style progressive cavity displacement pump) through a suitable heat exchanger having sufficient capacity to elevate the temperature of the beef fat stream to a pasteurizing temperature preferably at above 170°F or alternatively to a selected temperature of less than 108°F and then via a very high "G" force decanter centrifuge (10,000G) wherein beef tallow and any free or bound water is separated from the solids to provide three streams comprising a first stream of liquid beef tallow, a second stream of water and a third stream of connective tissue.

The "bound" as well as any "free" water that may have been present is separated from the stream of heated beef fat then combined with the fluid water separated from the beef fat particles and connective tissue in the fat flotation tank. The combined stream of fluid is then transferred via a second high "G" force centrifuge wherein creatine, blood components and other health supplement raw materials are separated from the fluid stream and then dewatered.

Referring now to FIGURE 3, a cross section through an open topped flotation vessel 200 (also referred to as a separator) is shown and interfaced with other items of equipment all controlled by PLC (programmable logic controller) to operate in sequence according to a specially written program and capable of separating a single input material stream of suspended solid beef fat particles 304 and 366 and particles of lean beef and connective tissue 324, suspended in the fluid 311, into several streams including a first stream of fluid, a second stream of beef fat 366 including some connective tissue and a third stream of lean beef and beef connective tissue 324. In some embodiments, the floatation vessel 200 allows for combining the material comprising a separable fat component with a fluid comprising aqueous carbonic acid, liquid carbon dioxide and water, an aqueous alkaline solution with nanobubbles, or an aqueous acid with nanobubbles, or any other microbiocidal agent listed herein with nanobubbles, such that the density of the fluid is greater than the density of the fat component of the material, allowing the fat component from the material to separate from the material and to stratify forming a first stratum in the fluid, thereby leaving a reduced fat component of the material, and allowing the reduced fat component to stratify forming a second stratum in the fluid; and collecting the second stratum comprising reduced fat component.

The open topped vessel 200 comprises an enclosure having a rectangular plan view profile with three flat vertical sides and one flat side 371 disposed at outwardly angled, all as shown in FIGURE 3, with lateral, rigid baffles such as 310, 308, 330 and 326 fixed across vessel 200 and between two opposing vertical vessel sidewalls. The bottom of vessel 200 is enclosed with a base having a corrugated profile and comprising a series of peaks and troughs such as peak 303 and troughs such as 301 wherein each peak 303 and trough 301 is connected by two flat sides such as 306 with each side being disposed at suitable angle as shown (at about 60°) thereby creating "V" shaped corrugations across the bottom section of the vessel 200. All of the corrugated peaks have a common height and are level at the same altitude with lower troughs such as 301 which are arranged with bases at a similar level across the base of the vessel 200. In this way, each trough collects an accumulated quantity of dense beef particle sediment 324 as the particles are allowed to settle after transfer into vessel 200 via pipe 374. The vessel 200 with profiled bottom can be filled with the fluid suspension 311 to a level shown by broken line 312. Fluid 311 with beef particles such as 324, 307 and 304 substantially fills the open topped vessel 200 to level 312 in such a manner that light phase beef fat particles 307 are able to float at the fluid 311 surface level 312. The vessel 200 is supported on legs such as 382 arranged to carry the weight of the vessel 200 when filled with fluid 311.

The upper level 312 is adjustable by adjusting the height of baffle plate 202 which is fixed in position after any adjustment to provide a suitable fluid level 312.

The fluid suspension 311 with beef particles 307, 304 and 324 is transferred by pumping in a continuous stream from the end of pipe section 83 shown in FIGURE 1 directly into space 372 of inlet pipe 374 in the direction shown by arrow 376. The fluid rate of mass flow can be any convenient rate of flow but most suitable would be in the range of 200gpm to 400gpm or more. The velocity of the fluid suspension transferred into vessel 200 via pipe 374 slows substantially after it has entered the vessel which is aided by lateral baffles 330, 326 and 308. This facilitates beef particle stratification such that the less dense fat particles shown as 307 steadily float upward while sedimentation of the more dense beef particles such as 324 facilitates accumulation in the corrugated troughs 301 at the bottom of vessel 200. A paddle assembly comprising a rigid frame 362 enclosing conveyor belt 348, which is held taught and captive by end rollers 314 and 358 such that conveyor belt 348 is tensioned by support rollers 320, 342, and 346. The conveyor belt 348 has a series of paddles such as 364 and 344 fixed thereto and spaced apart equally. Conveyor assembly with frame 362 is mounted horizontally above the open topped vessel 200 such that paddles 344 can travel with the conveyor belt 348 which can be driven by a variable speed electric motor (not shown) at a suitably steady rate. Paddles 344 are profiled with a suitable curve and the entire assembly is arranged so that the lower lengthwise edge of the paddles 344 penetrate the fluid surface 312 illustrated by paddle member 336. The fluid 311 surface level 312 with beef fat particles 307 floating at surface fills the vessel 200 up to a suitable elevation such that surface level 312 intersects a ramp member 350 which extends above the fluid level 312. The conveyor belt 348 with paddles 344 can be driven in the direction shown by arrows 316, 318 and 354 such that the paddles 336 sequentially penetrate the fluid surface as each paddle travels around end roller 314 at the left hand end of vessel 200 then moving toward the ramp member 350 which is rigidly fixed at the right hand end of vessel 200. Ramp member 350 extends the width of the vessel 200. Paddles represented by 344 and 336 extend lengthwise, with a vertical disposition, across the width of vessel 200 so that as the paddles 344 travel from left to right in the direction shown by arrows 316, 318 and 354, each paddle carries or pushes a quantity of floating fat particles such as 307 toward the ramp member 350. The quantity of beef fat particles carried by each paddle will therefore steadily increase as the paddles are driven across the fluid surface 312 in the direction shown.

The continuous conveyor belt 348 is held taught and follows a fixed path dictated by the retaining rollers 314, 358, 320, 342 and 346 and in this way the section of conveyor belt 356 held taught between rollers 346 and 358 can be maintained parallel to the ramp section 350. This configuration ensures that the lower paddle edge as shown at 336 of each paddle 344 does not collide with or contact the flat ramp section 350, however the configuration allows a close proximity of edge 336 to the flat upper surface of ramp member 350 as the paddles are driven up the ramp 350, each transferring a quantity of beef fat upward following the ramp 350 and lifting the beef fat away from the fluid 311.

It can therefore be readily understood that beef fat particles such as 366 can be separated from the fluid such as 311 in the manner described herein above. A retaining member 368 is arranged at a convenient location adjacent to the outer edge and underside of ramp 350, so as to conveniently provide a guiding effect to the continuous "waterfall style stream" of the beef fat particles 366 as the stream drops over the ramp edge downwardly and then onto sieve member 392.

The gravity-fed sieve member 392 comprises a sheet of perforated stainless steel having a curved profile and is disposed at a relatively steep angle such that beef particles 366 and 400 are impeded but not held as the beef fat particle stream falls in the direction shown by arrows 370, 396, 398 and 402. This configuration facilitates a contacting of the beef particles with the perforated member 392 but does not stop movement of the particles. In this way, excess fluid 390 which may be carried with beef particles 366 up ramp 350 can be separated without allowing the beef particles to fill the perforations which could otherwise quickly block the perforations and in so doing prevent continuous separation of the excess fluid 390 which, with this arrangement, can penetrate the perforations and fall in the direction shown by arrow 394 into trough member 406. The fluid collected in trough member 406 can be transferred via pipe 434 in the direction shown by arrow 436 and either discarded or combined with fluid extracted via pipe207 in the direction shown by arrow 208.

The stream of beef fat solids 400 is collected in a retaining member 408 and then transferred, under elevated pressure, via a Seepex "Moyno" style positive displacement ("PD") pump 410 directly into pipe 412 in the direction shown by arrow 404, through a suitable heat exchanger 414 where the temperature of the beef fat particles 400 stream is elevated to not more than 108°F or greater than 160°F depending upon it's intended use, and then the stream is transferred directly into centrifuge 420 via pipe 416 in the direction shown by arrow 418. The stream of temperature elevated beef fat is then divided into 3 streams comprising a first stream of liquid beef tallow which is extracted via a pipe 424 in the direction shown by arrow 422, a second stream of water via pipe 428 in the direction shown by arrow 426 and a third stream of lean beef solids comprising substantially all connective tissue via pipe 417 in the direction shown by arrow 415.

Referring again to FIGURE 3, a series of vertically disposed pipes 282, 284, 286, 288, 290, 292, 294, and 296 are each connected via open ports directly to each trough of the vessel corrugated bottom and most preferably at the lowermost level of each trough. Valves 234, 244, 250, 256, 262, 268, 274, 280, and 386 respectively are arranged to open or close such that fluid with accumulated beef solids such as 324 can be extracted from each trough separately and individually such as from trough 301. Each pipe 282, 284, 286, 288, 290, 292, 294, and 296 then connects to a common manifold pipe 232 running longitudinally along the bottom of the vessel 200. The outlet pipe 207 discharges fluid from a section on the vessel provided with a trap 204 that dips below the inlet to pipe 207 to collect any particulate matter to avoid carrying over the particulate matter in the fluid leaving the vessel 200 through pipe 207. The trap 204 is made from an upright baffle 206 that extends below the lower edge of the pipe 207. The bottom section 214 of the trap 206 is emptied through pipe 220 in the direction of arrow 212. The pipe 220 has an upper section 216 connected to a lower pipe section 222 via a valve 218. The lower pipe section 222 in turn connects to the pipe section 232 and forms a combined pipe 226. A suitably sized variable speed sine pump 230 is connected directly to the end of pipe section 226 and arranged to pump fluid with accumulated solids from pipe section 232 and pipe section 220 in the direction shown by arrow 430. As can be appreciated, the pump 230 can be used to pump fluid and solids extracted from any one or more of the troughs and the trap.

The (either open or closed) valves 234, 244, 250, 256, 262, 268, 274, 280, 386 and 218 can be arranged to be normally closed and sequenced such that only one valve is open at any given time for an adjustable period, preferably about 15 seconds. The valve opening sequence can be programmed into a PLC controller used to control the valve opening and closing such that during every 150 second period each valve is open while all other valves are closed. In this way the full available pumping force of pump 230 is applied to the extraction of fluid and solids such as 301 individually from each trough section of the corrugated bottom of vessel 200.

All excess fluid remaining after separation of substantially all solids have been separated and extracted via ramp 350 or any of the 10 valves is extracted via space 210 via pipe 207 in the direction shown by arrow 208 and transferred for filtering via transfer through a high "G" force centrifuge(s) and then further processed via the nanobubbles process wherein chorine gas, chlorine dioxide or carbon dioxide is provided in nanobubble condition into the fluid prior to recycling and generally as described herein above prior to temperature reduction (or temperature elevation according the required fluid temperature) and re-cycling through any of the pipes shown as 5, 14 or 35 in FIGURE 1. Referring now to FIGURE 2, a cross section through a decanter style, centrifuge sub-assembly comprising the bowl 142, scroll 140 with flights 146, 132 and 118, feed tube 130 and drive members 100 and 129 is shown. The scroll 140 comprises left hand and right hand screw flights such as 118, 132 shown at the left hand end of the scroll 140 while flights 113 and 149 of an opposite hand are located at the opposite end of the scroll 140. The flights are ribbon-shaped, such that the outer ribbon edge is in close proximity to the bowl surface 111. The ribbon is connected to the scroll 140 in a manner to create openings between the ribbon and the scroll such that material that is not at the bowl surface that is suspended in the liquid can travel between the openings through the ribbon to the opposite end of the centrifuge. During the centrifuge separation process, heaver solids 120 are at the surface of the bowl and are carried up the ramp 116, while less dense and suspended solids 106 not at the bowl surface are transferred through the openings in the ribbon and are expelled at the opposite end of the bowl 142 through opening 105, 141.

The centrifuge sub assembly is shown without a main frame, mounting fixtures, independent drives, controls and typical guarding so as to facilitate a clear view of the centrifuge separation mechanism.

Typically, when a decanter centrifuge is used to separate beef trim into its components comprising beef fat (tallow) and lean beef, the beef trim is ground and then heated to a suitable temperature of about 108°F prior to transfer into the decanter centrifuge. In this way the beef fat or tallow is liquefied while the lean beef and connective tissue components remain in a solid condition and the liquid fat can be readily separated from the beef solids in a decanter style centrifuge. However, the present invention does not include the sequence of heating the beef trim prior to centrifuging. In fact, the beef trim is cut into particles, frozen and crushed to separate beef fat particles from lean beef and connective tissue particles. The beef particles are then combined with a selected fluid also at low temperature. Accordingly, the purpose of this particular embodiment (i.e., wherein a scroll having left hand and right hand scroll flights is incorporated in the separation mechanism) is to enable the separation of a low temperature suspension comprising a mixture including a fluid 135 and 112 such as water or an acid solution or alkaline solution with solid beef particles 120 wherein a first, predominant portion of the solid beef particles comprises beef fat, a second proportionately lesser quantity comprises lean beef particles and/or connective tissue particles and a third lesser portion of beef particles 106 comprises any combination of fat and lean or fat, lean and connective tissue or fat and connective tissue.

The centrifuge enables the separation of the suspension 112 into particles 106 and 120 which is transferred into the centrifuge assembly via static tube 130 through space 126, into two streams wherein a first stream comprises lean beef (including a proportion of connective tissue) 120 and a second stream of fluid 112 and 135 combined with particles 106 comprising any combination of fat, fat and lean or fat, lean and connective tissue or fat and connective tissue.

The quantity of the particles 106 separated with the fluid 112 and 135 may be substantially less in volume than the quantity of lean particles 120.

The equilibrated temperature of the second stream of fluid after processing via equipment described herein in association with FIGURE 1 including suspended particles 106 as well as the first stream of lean particles 120 is less than 44°F and most preferably the fluid will contain nanobubbles otherwise known as paramagnetic bubbles.

The bowl 142 is mounted on suitable bearings and rigidly attached to drive member 100. Scroll 140 is also mounted on suitable bearings and rigidly attached to drive member 129. In this way the bowl assembly 142 and 100 as well as the scroll assembly 140 and 129 can spin freely and independently. A cone shaped ramp 104 is arranged at the inner, end region of the bowl at an end thereof and a cone shaped ramp 116 comprises a section of the bowl and rigidly fixed thereto at the opposite end of the bowl to ramp 104.

The density of lean beef particles 120 is about 66 lbs/cu', the density of the fluid 112 and 135 is about 62.4 lbs/cu' while the density of the suspended particles 106 is about the same as the fluid 112 and 135 or slightly more or less.

During operation of the decanter style centrifuge, the bowl 142 is preferably driven at about 2,000rpm while the scroll 140 is preferably driven at about 2025 rpm, thereby providing a speed differential of 25rpm such that the scroll is preferably rotating at 25 rpm greater than the bowl, however, the speed of the independently driven bowl 142 and scroll 140 can most preferably be varied as can be the differential speed between the bowl 142 and scroll 140.

The lean particle stream is transferred via ports 137 and 123. A gas such as air or carbon dioxide is provided to fill the space 110, 114 and 145 closest to the scroll 140. The gas can pass through ports 137 and 123. The centrifuge of FIGURE 2 can be used for centrifugally spinning a mixture of meat components, a fluid with or without nanobubbles, and optionally, including at least a microbiocidal agent such as, one or more of the microbiocidal agents can include one or more of hypochlorous acid, hydrochloric acid, bromine, fluorine, any halogen, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide, to separate meat components in concentric zones according to density, wherein denser components accumulate farther away from the axis of rotation and less dense components accumulate closer to the axis of rotation; and then transferring denser components towards a first cone-shaped section of the centrifuge via a first screw action and transferring less dense components towards a second cone-shaped section of the centrifuge via a second screw action, wherein gas can accumulate at zones in the proximity of the cone-shaped sections so as to impede the fluid from exiting with the meat components. In other embodiments, after separating the majority of the fat solids in the manifold 38, and transferring fluid and solids removed via the lower outlets, the centrifuge of FIGURE 2 can be used to centrifugally spin the fluid to individually separate the lean meat solids and the fluid with some fat particles, wherein the lean meat solids, and the fluid with some fat solids are separated in the same centrifuge.

During operation of the decanter centrifuge shown in FIGURE 2, a suspension comprising fluid (as described above) with solids is transferred via static tube 130 through space 126 in the direction shown by arrows 128 and 134 and into chamber 144 which is rotating at the same speed as the scroll 140. Centrifugal force causes transfer of the suspension through passageway 138 in the direction shown by arrow 136 and into space 114, 145 and 110. The bowl 142 and scroll 140 preferably both rotate in the same direction while the scroll rotates at a speed equal to about 25rpm greater than the speed of the bowl. In this way the fluid with beef particles rapidly occupies a space closest to the inner surface 111 and 147 of the bowl 142 thereby creating a pool 112 with a surface 108 parallel to the inner surface 147 and 111 of bowl 142.

As the fluid with suspended beef particles is continuously forced against the inner surface 11 1 and 147 of the bowl 142, the gravitational force being applied causes the more dense lean beef particles 120 to quickly occupy space closest to the bowl 142 inner surface 111 and 147, while the fluid occupies the space shown by 112 and surface 108, and the remaining beef particles 106 which comprise more fat occupy locations between the inner fluid surface 108 and the bowl inner surface 111 and 147, while the fluid flows in the direction shown by arrows 141 and 109. The depth of the fluid is controlled by the location of ports shown as 139 and 105 which are preferably a group of concentrically arranged round ports positioned in an annular pattern centered around the centerline 99 with the distance between the inner surface of the bowl 111 and 147 and a circular line profile (108) which tangentially contacts the closest point of each port closest to the centerline 99 equal to the fluid pool 112 depth.

Fluid therefore exits via ports 105 and 139 and is inhibited from exiting ports 123 and 137 by gas occupying space 110 and cone shaped ramp 114.

Lean beef particles 120 are carried, in the direction shown by arrows 133 and 124 up the incline provided by cone shaped ramp 116 and then in the directions shown by arrows 131 and 122 after exiting the ports 137 and 123 caused by the action of flights such as 118 and 132 of scroll 140 which preferably rotates at 25rpm greater than bowl 142. However, both suspended and floating particles 106 are carried with the fluid 112 which flows toward apertures 105 and 139. The floating and suspended particles 106 are then carried up the ramp 104 by the action of the flights 149 and 113. In the absence of suitably profiled and handed flights 149 and 113, the particles 106 can create a porous dam which progressively builds while allowing fluid to flow, because the adhesion to the ramp 104 caused by the centrifugal force cannot be overcome by the force provided by the flow of the fluid.

FIGURE 4 shows one embodiment of a method for separating lean and fat from meat, beef, or proteinaceous material.

Block 502 is a step for cutting, dicing, grinding, or otherwise reducing the size of beef, meat, or other proteinaceous material that has fat and lean. After cutting, dicing, or grinding, the average size of the pieces of meat are in the order of about 1 inch across. However, there can be variation in the average size of from 0.1 inch up to 3 inches or more. From block 502, the method enters block 504.

Block 504 is a step for cooling, chilling, freezing, or otherwise reducing the temperature of the pieces of meat coming from block 502. The apparatus for cooling is described as cooling or quick freeze tunnel (item 4 in FIGURE 1). Rapid cooling and different heat capacities for lean and fat result in a difference in temperatures of the fat and lean within each piece. By adjusting the exposure time to a chilling cryogenic gas or the temperature of the chilling gas or both time and temperature, it is desired that the temperature of lean be about 10°F to26°F and the temperature of the fat be about -5°F to 2°F. At these temperatures, the fat will crumble while the lean remains flexible when subjected to a crushing force. From block 504, the method enters block 506.

Block 506 is a step for crushing the cooled pieces of meat coming from the cooling tunnel. The crusher (items 20 and 21 in FIGURE 1) uses two rotating rollers separated by a gap of from 1/32" to 1.00" inches but most preferably about 1/16". The cooled pieces of meat with fat and lean at the temperatures described above pass in between the rollers to be crushed, thus, liberating most or some of the fat from the lean, resulting is particles of fat that are mostly or predominantly fat and particles of lean that are mostly or predominantly lean. From block 506 the method enters block 508.

Block 508 is step for mixing the particle of fat, particle of lean, with a fluid. In one embodiment, the mixing uses a cone-shaped vessel (item 24 in FIGURE 1) capable of creating a vortex with a fluid, block 428, injected tangentially to the wall to create a vortex. The fluid is the fluid described in association with element 5 in FIGURE 1 and can be an aqueous fluid with a microbiocidal agent, and further include nanobubbles filled with a paramagnetic gas such as oxygen contained in air. From block 508 the method enters block 510.

Block 510 is a step for a first separation of lean particles from the fat particles and fluid. In one embodiment, the apparatus used is a manifold (item 38 in FIGURE 1 or item 700 in FIGURE 7). The manifold is generally a pipe having a vertical section and a horizontal section. In an embodiment, the manifold 38 has outlets on the underside of the horizontal section. In the manifold 700 of FIGURE 7, the outlets 702 are placed on the top side of the manifold and there are no outlets on the underside of manifold 38 of FIGURE 1. The outlets 702 on the top side are used to collect the fat, which is sent to the flotation tank separator (item 200 in FIGURE 3). In the manifold 700, the lean particles travel with fluid along the horizontal section and are then fed to a centrifuge for separation of the fluid from the lean particles. Either type of manifold may also include one or more inlets for injecting additional fluid for temperature control and achieving a suitably selected solids to fluid ratio for good separation. Preceding with the manifold 38 of FIGURE 1, the lean particles being denser than the fluid will settle and be collected by the outlets provided underneath the horizontal section of the manifold. Suspended particles and fluid continue to travel horizontally along the manifold. At this point, the solids in the manifold are mostly fat. The fluid with fat particles is transferred to a floatation tank, block 522. The flotation tank (FIGURE 3) separates the fat from the fluid by allowing the fat to float to the surface of the fluid and then skimming the surface to collect the fat. In the floatation vessel, any lean that happens to collect will also be recovered. The fluid is likewise recovered, block 534, and can be processed for re-use. From block 522, the method enters block 524.

Block 524 is step for rendering the fat by the application of heat in a heater (item 414 in FIGURE 3). The heat is able to render down the fat into three main constituent components, including tallow, solids, and water. After block 524, the method enters block 526.

Block 526 is a step for centrifugally spinning the materials rendered from the fat using a decanter centrifuge (item 420 in FIGURE 3), for example. The decanter centrifuge is able to separate tallow and any free or bound water from the solids to provide three streams comprising a first stream of liquid beef tallow, a second stream of water and a third stream of connective tissue. Alternatively, water and solids may be separated together in a first stream with the liquidized tallow separated in a second stream.

Referring back to block 510, the lean particles that are recovered with some amount of fluid are transferred to block 520.

Block 520 is a step for centrifugally spinning the lean particles and fluid in a decanter centrifuge (FIGURE 2). The decanter centrifuge is able to separate two streams of lean and fluid containing any matter that is suspended in the fluid. Fluid, block 532, may be introduced into the decanter centrifuge to achieve the required level of separation.

In an embodiment, fluid recovered from centrifuges, blocks 520 and 526, can also be re-use.

In FIGURE 4, the fluid blocks 528, 530, and 532, can originate from the same source, or alternatively each can have a different source. The fluid of block 528, 530, and 532 can be any fluid described herein, with or without nanobubbles, and optionally including one or more microbiocidal agents such as one or more of hypochlorous acid, hydrochloric acid, bromine, fluorine, any halogen, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide.

Fluid that can be recovered can be re-used in the system after treatment. Make-up potable water, block 542, can be combined with the recovered fluid. To prepare the anti- microbial fluid used in the CBRS, the potable water, block 542, is combined with a quantity of carbon dioxide, block 536. The carbon dioxide dissolves in the potable water sufficient to adjust (typically reduce) the pH of the water to a value within the range of 4.0 to 5.5. Then, the pH adjusted fluid is transferred through a nanobubble generating device, block 536. In an embodiment, the nanobubble generating device is the tower illustrated and described with reference to FIGURES 5 and 6. However, other embodiments of a nanobubble generating device are contemplated. The pH-reduced fluid with the nanobubbles is then combined with hypochlorous acid (or a quantity of chlorine gas), block 538, such that the resultant free chlorine content of the fluid that comes into contact with the beef particles is within the range of 3 ppm to 50 ppm. After treatment and processing of the make-up water, the water can be combined with the recycled fluid and pumped to the various users, including the vortex vessel and manifold, for example.

Referring to FIGURE 10, a system is illustrated similar to the system illustrated in FIGURE 4. In general, the two systems are similar in many respects. In FIGURE 10, a system for separating fat from lean includes at the front end a combo dumper 902, a main grinder 904, an inclined conveyor 906 leading to a nitrogen cooling tunnel 908. As explained above, the nitrogen cooling tunnel cools the pieces of meat, which then feed into the bond breaker or crusher 910. The bond breaker 910 breaks the fat apart from the meat, but, leaves the lean meat largely intact. From the bond breaker, the fat and lean enter a vortex, where the fat and lean is mixed with a fluid. The fluid includes recycled fluid and make-up fluid that is treated to contain nanobubbles and has an acidic pH and chlorine 958. Make-up fluid comprising fresh water 914 is pumped with the recycled fluid, and the combined fluids are injected into the vortex 912. After the vortex 912, the sine pump 916 controls the liquid level to prevent air from entering the system of the open top vortex vessel 912. The sine pump 916 pumps the fluid mixture into separation manifold 1 (920) and a second sine pump 918 pumps the fluid mixture into separation manifold 2 (922). Two or more separation manifolds are placed in parallel to increase capacity. As described, the manifolds can be either item 38 of FIGURE 1 with bottom side outlet ports or item 700 of FIGURE 7 with top side outlet ports. The combined collected material from manifolds 920 and 922 are pumped via pumps 924, 926, 928, and 930 into a balance tank 932 for mixing. In FIGURE 10, the lean particles collected from the dual manifolds are combined in a balance tank with the solids that settle in the troughs at the bottom of the flotation vessel (FIGURE 3). From the balance tank 932, the solids are sent to the decanter centrifuge 932 where the lean is separated and the fluid is sent back to the flotation vessel 942 (item 200 of FIGURE 3). The lean collected from the decanter centrifuge 936 is conveyed via conveyor 940 and stored in a combo dumper 948.

The fat and fluid from the dual manifolds 920 and 922 is sent to the flotation tank 942, where the fat is collected in the manner described in association with FIGURE 3. Thereafter, the fat can simply be collected in a combo dumper 946. The fluid from the flotation tank 942 is pumped via pump 950 through a series of heat exchangers 952, 956 to cool the fluid. From the heat exchangers, the fluid can be combined with the make-up fresh water 914. As described, the fresh water is treated to adjust the pH, to contain nanobubbles, and with chlorine.

Referring to FIGURE 7 again, manifold 700 with outlets 702 on top to remove fat is further shown incorporated into a system. The manifold 700 transfers the fluid with lean particles and any remaining fat particles into a vessel 704. Then, the fluid and particle mixture is pumped via pump 706 into a two-cone decanter centrifuge 708. The decanter centrifuge separations the lean particles as a separate stream. The decanter centrifuge separates the fluid and fat particles as s separate stream, which then enters the gravity flow screen filter 714 to separate the fluid from the fat particles. Then, both the lean particles and the remaining fat particles are loaded on a conveyor 710 and transferred to a collection bin 712. An embodiment of a gravity flow screen separator is illustrated in FIGURE 12. As shown, the gravity flow screen separator has a screen 716 with a steep angle which gradually declines with lowering elevation. As the fluid and fat mixture is deposited on the top of the screen, the fluid passes through the perforations made in the screen 714, while the solid particles do not pass through the perforations and slide down the screen and are collected separately from the fluid.

Referring to FIGURE 13, an enclosure cabinet 12218 is located on along the path of travel of carcasses, such as carcass 12212, wherein carcass 12212 can be made to pass into enclosure 12218 and be enclosed within the cabinet 12218 while still suspended from a rail. Cabinet 12218 includes vertically disposed sides 12218 arranged in relative close proximity to the carcasses as they are transferred along rail 2208 and in such a manner so as to substantially retain any gas or liquid that may be sprayed within said enclosure. In an embodiment, "air curtains" 12220 and 12222 supplied by blowers or vacuums are mounted at each upper end of the enclosure and arranged to minimize escape of any gas or substances that may be sprayed within the enclosure 12218. A lower side cover 12228 with a drain mounted therein is located along the lower section of the enclosure 12218 and nozzles 12224 are provided in the side 12218. Nozzles 12224 can be used to inject fluids with microbiocidal agents, such as hypochlorous acid, hydrochloric acid, bromine, fluorine, halogen, chlorine, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide. In an embodiment, the nozzles 12224 are used to spray a fluid containing nanobubbles and a chlorine content in the range of 3 ppm to 50 ppm. A vent 12226 is mounted at the upper side of the enclosure 12218 and a powered extractor fan or impeller can be provided in such a manner so as to cause the extraction of any gases or vapors from within the enclosure 12218 as may be required. A drain may be used for disposing of the fluid or alternatively collecting the fluid after use then recycling the reclaimed fluid after removing all solids and pasteurizing the fluid by firstly elevating the fluid temperature to greater than 160° F followed by chilling the fluid to a temperature below 160°F prior to reuse in the make-up fluid or pressurizing the fluid to a pressure greater than 80,000psi.

As can be appreciated high levels of purification of lean meat and tallow can be achieved. The lean meat and tallow can be used in a number of products. The lean meat can be combined with other meats, such as ground beef, and packaged. Additionally, control of the water content is practiced so that the packaged meats contain the appropriate amount of water or does not exceed the mandated amount of added water. An advantage of the fluid is to provide a process that is free of reduced populations of microbes or pathogens.

Based on the foregoing disclosure, representative embodiments include, but are not limited to the following.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material such that the fat is rigidly frozen and is friable but lean meat and remains flexible; reducing the lean meat-containing material into particles, wherein the particles include particles that have a majority of lean meat and generally smaller particles that have a majority of fat; combining the particles with a fluid, wherein the fluid includes nanobubbles, and the fluid includes water and one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, halogen, chlorine, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide; generating the nanobubbles in a tower having semispherical baffles arranged along a length of the tower; and collecting particles that float in the fluid or collecting particles that sink in the fluid. In an embodiment, the method further comprises transferring a majority of the fluid with the particles that were not collected and separating the majority of the fluid.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material so as to rigidly freeze the fat while the lean meat remains flexible; reducing the lean meat-containing material into particles, wherein the particles include particles that have a majority of lean meat and particles that have a majority of fat; generating gas nanobubbles in a fluid by passing the fluid through a tower having semispherical baffles arranged along a length of the tower; combining the particles with the fluid containing the gas nanobubbles; and collecting particles that float in the fluid or collecting particles that sink in the fluid. In an embodiment, the method further comprises transferring a majority of the fluid with the particles that were not collected and separating the majority of the fluid.

In an embodiment, a method for separating fat particles from lean particles, comprises providing beef pieces, wherein the beef pieces comprise fat and lean; lowering the temperature of the diced beef pieces, wherein the fat is reduced to a first temperature at which the fat is rigid and friable while simultaneously achieving a second condition for the lean at which the lean is less rigid and substantially flexible; crushing the beef pieces to liberate the fat into small separated particles without substantially fracturing lean and creating fat particles and lean particles; generating gas nanobubbles in a fluid by passing the fluid through a tower having semispherical baffles arranged along a length of the tower; combining the fat particles and the lean particles with the fluid containing gas nanobubbles to provide a mixture; and collecting particles that float in the fluid or collecting particles that sink in the fluid. In an embodiment, the method further comprises transferring a majority of the fluid with the particles that were not collected and separating the majority of the fluid.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material while avoiding completely freezing the lean meat; such that the fat is rigidly frozen and is friable but lean meat is not frozen rigidly and remains substantially flexible when transferred between crushing rollers; reducing the lean meat-containing material into particles, wherein the particles include particles that have a majority of lean meat and particles that have a majority of fat; combining the particles with a fluid, wherein the fluid includes water and one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, halogen, chlorine, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide; transferring the fluid and particles through an elongated vessel aligned horizontally; collecting particles that float in the fluid from the top of the vessel; continuing to transfer a majority of the fluid with the particles that were not collected; and separating the majority of the fluid.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material such that the fat becomes rigidly frozen while the lean meat remains flexible and does not shatter when subjected to a crushing force; reducing the lean meat-containing material into particles, wherein the particles include particles that have a majority of lean meat and particles that have a majority of fat; combining the particles with a fluid; transferring the fluid and particles through an elongated vessel aligned horizontally; collecting particles that float in the fluid from the top of the vessel; continuing to transfer a majority of the fluid with the particles that were not collected; and separating the majority of the fluid. In an embodiment, a method for separating fat particles from lean particles, comprises providing beef pieces, wherein the beef pieces comprise fat and lean; lowering the temperature of the beef pieces, wherein the fat is reduced to a first temperature at which the fat is friable while simultaneously achieving a second temperature for the lean at which the lean is flexible; crushing the beef pieces to liberate the fat without fracturing lean and creating fat particles and lean particles; combining the fat particles and the lean particles with a fluid to provide a mixture; transferring the mixture through an elongated vessel aligned horizontally; collecting particles that float in the fluid from the top of the vessel; continuing to transfer a majority of the fluid with the particles that were not collected; and separating the majority of the fluid.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material while avoiding completely freezing the lean meat; while the fat is rigidly frozen, is friable and fractures when subjected to a crushing force but lean meat remains flexible and is not substantially size reduced when subjected to the same crushing force as the fat; reducing the lean meat-containing material into particles, wherein the particles include particles that have a majority of lean meat and smaller particles that have a majority of fat; combining the particles with a fluid in a vortex vessel, wherein the fluid includes water and one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, halogen, chlorine, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide; discharging the fluid and particles from the vortex vessel into a conduit, wherein the conduit is connected to an outlet of the vortex vessel; controlling a level of fluid in the conduit to prevent the introduction of air; transferring the fluid and particles through an elongated separation vessel aligned horizontally which may have slightly upward path so that any air in the elongated separation vessel will move in a direction away from the vortex; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material while such that the fat becomes rigid and the lean meat is flexible; reducing the lean meat-containing material into particles, wherein the particles include particles that have a majority of lean meat and smaller particles that have a majority of fat; combining the particles with a fluid in a vortex vessel; discharging the fluid and particles from the vortex vessel into a conduit, wherein the conduit is connected to an outlet of the vortex vessel; controlling a level of fluid in the conduit to prevent the introduction of air; transferring the fluid and particles through an elongated separation vessel aligned horizontally; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for separating fat particles from lean particles comprises providing beef pieces, wherein the beef pieces comprise fat and lean; lowering the temperature of the diced beef pieces, wherein the fat is reduced to a first temperature at which the fat is friable while simultaneously achieving a second temperature for the lean at which the lean is flexible; crushing the beef pieces to liberate the fat without fracturing lean and creating fat particles and lean particles; combining the fat particles and the lean particles with a fluid in a vortex vessel to provide a mixture; discharging the mixture from the vortex vessel into a conduit, wherein the conduit is connected to an outlet of the vortex vessel; controlling the level of fluid in the conduit to prevent the introduction of air; transferring the fluid and particles through an elongated separation vessel aligned horizontally; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material while avoiding completely freezing the lean meat; while the fat is rigidly frozen and is friable but lean meat remains flexible; crushing the chilled lean meat-containing material between a first and second roller to produce particles that have a majority of lean meat and particles that have a majority of fat, wherein the first and second rollers have teeth on a periphery, wherein the teeth have a repeating curving wave pattern; combining the particles with a fluid, wherein the fluid includes water and one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, halogen, chlorine, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide; transferring the fluid and particles through an elongated separation vessel aligned horizontally; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material while such that the fat becomes rigid and friable but the lean meat remains flexible; crushing the chilled lean meat-containing material between a first and second roller to produce particles that have a majority of lean meat and particles that have a majority of fat, wherein the lean particles are larger than the fat particles and the first and second rollers have teeth on a periphery, wherein the teeth have a repeating curving wave pattern; combining the particles with a fluid; transferring the fluid and particles through an elongated separation vessel aligned horizontally; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for separating fat particles from lean particles, comprises providing beef pieces, wherein the beef pieces comprise fat and lean and are size reduced; lowering the temperature of the beef pieces, wherein the fat is reduced to a first temperature at which the fat is friable while simultaneously achieving a second temperature for the lean at which the lean is flexible; crushing the chilled beef pieces between a first and second roller to liberate the fat without fracturing lean and creating fat particles and lean particles, wherein the first and second rollers have teeth on a periphery, wherein the teeth have a repeating curving wave pattern; combining the fat particles and the lean particles with a fluid to provide a mixture; transferring the mixture through an elongated separation vessel aligned horizontally; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material while avoiding completely freezing the lean meat; while the fat is rigidly frozen and is friable but lean meat is not frozen rigidly and remains flexible; reducing the chilled lean meat-containing material into particles that have a majority of lean meat and particles that have a majority of fat; preparing a make- up fluid comprising water by adjusting pH from 4.0 to 5.5, by mixing the fluid with a measured quantity of carbon dioxide gas, then transferring the fluid through a conduit within which cavitation is provided to create nanobubbles in the fluid, and adding chlorine to a level of 3 ppm to 50 ppm; combining the particles with the fluid, wherein the fluid includes water and one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, halogen, chlorine, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide; transferring the fluid and particles through an elongated separation vessel aligned horizontally; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; chilling the lean meat-containing material so that the fat becomes rigid and friable while the lean meat is flexible; reducing the chilled lean meat-containing material into particles that have a majority of lean meat and particles that have a majority of fat; preparing a make-up fluid comprising water by adjusting pH from 4.0 to 5.5, adding nanobubbles, and adding chlorine to a level of 3 ppm to 50 ppm; combining the particles with the fluid; transferring the fluid and particles through an elongated separation vessel aligned horizontally; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for separating fat particles from lean particles, comprises providing beef pieces, wherein the beef pieces comprise fat and lean; lowering the temperature of the diced beef pieces, wherein the fat is reduced to a first temperature at which the fat is friable while simultaneously achieving a second temperature for the lean at which the lean is flexible; crushing the chilled beef pieces to liberate the fat without fracturing lean and creating smaller fat particles and lean particles which are larger than the fat particles; preparing a make-up fluid comprising water by adjusting pH from 4.0 to 5.5, creating nanobubbles in the fluid, and adding chlorine to a level of 3 ppm to 50 ppm; combining the fat particles and the lean particles with the fluid to provide a mixture; transferring the mixture through an elongated separation vessel aligned horizontally; and collecting particles that float in the fluid from the top of the separation vessel or collecting particles that sink in the fluid from the bottom of the separation vessel.

In an embodiment, a method for reducing pathogen populations such as E. Coli 0157:H7 that may be present on the surface of meat pieces comprises providing meat pieces comprising lean meat and fat; chilling the meat pieces; preparing a make-up fluid comprising water by adjusting pH from 4.0 to 5.5, by mixing the fluid with a measured quantity of carbon dioxide gas then transferring the fluid through a sealed, modified conduit at such a rate and pressure causing cavitation to create nanobubbles in the fluid, and adding chlorine to a level of 3 ppm to 50 ppm; immersing the meat pieces in the make-up fluid with gentle agitation to ensure all meat piece surfaces are exposed to the fluid, wherein the make-up fluid includes water and one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, halogen, chlorine, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide; removing the meat pieces from the make-up fluid in a manner that results in no more than 0.5% added water to the meat pieces.

In an embodiment, a method for reducing pathogen populations such as E. Coli

0157:H7; other STEC's (Shiga toxin-producing E. Coli) and salmonella that may be present on the surface of beef carcasses following animal slaughter, prior to chilling and carcass disassembly; the method comprising providing freshly slaughtered beef carcasses suspended from a meat rail; providing a cabinet arranged to open and enclose around a suspended beef carcass; providing a series of fluid jets arranged around the inner walls of the cabinet and pointing inward; preparing a make-up fluid comprising water by adjusting pH from 4.0 to 5.5, by mixing the fluid with a measured quantity of carbon dioxide gas then transferring the fluid through a sealed, modified conduit at such a rate and pressure to cause cavitation and thereby generate nanobubbles in the fluid, and adding chlorine to a level of 3 ppm to 50 ppm; enclosing each carcass in the cabinet while still suspended from a meat rail; processing the carcass by transferring the make-up fluid under elevated pressure through the jets arranged inside the cabinet to direct the pressurized fluid onto the surface of the carcass, wherein the pressure of the fluid is sufficient to remove fecal matter, micro-organisms and all undesirable matter from the carcass surface, wherein the make-up fluid includes water and one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, halogen, chlorine, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide; following thorough processing within the cabinet, opening the cabinet to allow removal of the carcass and transfer of the carcass to a chiller; disposing of the fluid or alternatively collecting the fluid after use then recycling the reclaimed fluid after removing all solids and pasteurizing the fluid by firstly elevating the fluid temperature to greater than 160° F followed by chilling the fluid to a temperature below 160°F prior to reuse in the make-up fluid or pressurizing the fluid to a pressure greater than 80,000 psi.

In some embodiments, a method for separating lean meat from lean meat- containing material comprises reducing meat into particles; cooling the particles; after cooling, crushing the particles to break apart fat from the particles; mixing the particles and fat with a fluid spun into a vortex; transporting the mixture through a manifold and removing particles that sink from the bottom of the manifold, and transporting remaining mixture to a settling vessel; and transporting the particles that sink to a decanter centrifuge.

In some embodiments, the fluid is an aqueous fluid comprising water, a microbiocidal agent, and nanobubbles having a size of less than lOOnm. In some embodiments, the pathogen deactivating microbiocidal agent is dissolved in the water, and is not contained in the nanobubbles.

In some embodiments, the microbiocidal agents include one or more of hypochlorous acid, hydrochloric acid, bromine, fluorine, any halogen, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide.

In some embodiments, the method further comprises, in the settling vessel, individually separating fluid, fat and connective tissue.

In some embodiments, the method further comprises rendering the fat into a liquid by heating, and centrifugally spinning the liquid to individually separate liquid beef tallow, water, and beef solids.

In some embodiments, the method further comprises combining the particles that sink with fluid and then centrifugally spinning the fluid and particles in decanter centrifuge to separate lean from the fluid.

In some embodiments, the method further comprises spinning an inner scroll of the centrifuge at a higher rpm than an outer bowl of a centrifuge, and expelling lean beef particles at one end of the centrifuge, while expelling fluid and suspended or floating matter at an opposite end of the centrifuge.

In some embodiments, the scroll has left hand and right hand flights.

In some embodiments, in the vortex mixing step, the ratio of fluid to solids, including particles and fat, is at least 8 parts fluid to 1 part solids by weight or volume.

In some embodiments, the decanter centrifuge separates lean from the fluid and a separation time from mixing the fluid in a vortex vessel to separating the fluid from the lean in the decanter centrifuge is less than 3 minutes, or less than 90 seconds.

In some embodiments, the step of mixing the particles and fat with a fluid spun into a vortex further comprises measuring a weight to control a depth of the fluid/solids suspension.

In some embodiments, a method for separating lean meat from lean meat- containing material comprises providing lean meat-containing material having lean meat and fat; before reducing the lean meat-containing material into particles, chilling the lean meat-containing material while avoiding completely freezing the lean meat; while the fat is rigidly frozen and is friable but lean meat is not frozen rigidly and remains flexible; reducing the lean meat-containing material into particles, wherein the particles include particles that have a majority of lean meat and particles that have a majority of fat; combining the particles with a fluid, wherein the fluid with or without nanobubbles includes water and one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, any halogen, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide; introducing the particles and the fluid into a centrifuge after separating a majority of the fat particles; in the centrifuge, separating a first stream comprising the particles that have a majority of lean meat and a second stream comprising the fluid with a quantity of fat particles; separating the fat particles from the fluid and sanitizing the fluid and recycling the sanitized fluid; and treating the first stream comprising the particles that have a majority of lean meat to reduce pathogens via a method that does not result in raising the temperature above 109. degree. F.

In some embodiments, a method for separating lean meat from lean meat-containing material comprises providing lean meat-containing material having lean meat and fat; before reducing the lean meat-containing material into particles, chilling the lean meat-containing material while avoiding freezing the surface of the lean meat while the surface of the lean meat is non-frozen; reducing the lean meat-containing material into particles, wherein the particles include particles that have a majority of lean meat and particles that have a majority of fat; combining the particles with a first fluid, wherein the first fluid includes water and gas nanobubbles; introducing the particles and the first fluid into a centrifuge after separating a majority of the fat particles; in the centrifuge, separating a first stream comprising the particles that have a majority of lean meat and a second stream comprising some fat particles and the first fluid; sanitizing the first fluid and recycling the sanitized first fluid; and treating with a second fluid containing nanobubbles, the first stream comprising the particles that have a majority of lean meat to reduce pathogens via a method that does not result in raising the temperature above 44 degree. F.

In some embodiments, a method for separating meat components comprises combining fat solids and lean meat solids with a fluid comprising water and removing the majority of fat particles; after separating the majority of the fat solids centrifugally spinning the fluid by centrifuge; individually separating the lean meat solids and the fluid with some fat particles, wherein the lean meat solids, and the fluid with some fat solids are separated in the same centrifuge; controlling the temperature of the lean meat solids before separating; and controlling the temperature of separated fluid, and dividing the fat particles into a first stream of beef tallow and a second stream comprising substantially connective tissue.

In some embodiments, a method for separating fat particles from lean particles comprises providing diced beef pieces, wherein the diced beef pieces comprise fat and lean; lowering the temperature of the diced beef pieces to a first reduced temperature for the fat at which the fat is friable while simultaneously achieving a second reduced temperature for the lean at which the lean is flexible; crushing the beef pieces to liberate the fat without fracturing lean and creating fat particles and lean particles; combining the fat particles and the lean particles with a fluid containing gas nanobubbles to provide a mixture; introducing the mixture to an inlet of a chamber, wherein the chamber has an upper outlet and a lower outlet; allowing particles less dense than the fluid to be carried out from the chamber through the upper outlet with fluid; allowing the particles more dense than the fluid to be carried out from the chamber through the lower outlet with fluid; transferring the fluid with particles from the upper outlet to a separator wherein the particles are separated from the fluid and transferring the fluid with particles from the lower outlet to a centrifuge wherein the particles are separated from the fluid.

In some embodiments, a method for separating fat from beef comprises combining beef provided as small pieces with a fluid with or without nanobubbles comprising one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, any halogen, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide, in a separation manifold and creating turbulence in the manifold with the small beef pieces and the fluid allowing beef components comprising predominantly fat to rise to the top of the fluid in the manifold and beef components comprising predominantly lean beef to settle to the bottom of the fluid in the manifold; removing the beef components comprising predominantly fat from the fluid; and transferring the beef components comprising predominantly lean beef with fluid to a centrifuge.

In some embodiments, a method of reducing the fat content of a material comprises combining a material comprising a separable fat component with a fluid comprising one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, any halogen, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide, with or without nanobubbles, wherein the density of the fluid is greater than the density of the fat component of the material; allowing the fat component from the material to separate from the material and to stratify forming a first stratum in the fluid, thereby leaving a reduced fat component of the material; allowing the reduced fat component to stratify forming a second stratum in the fluid; and collecting the second stratum comprising reduced fat component.

In some embodiments, a method for separating fat from a material containing fat comprises combining a material with a fluid, with or without nanobubbles, comprising one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, any halogen, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide, wherein the material comprises components that comprise predominantly fat and components that comprise predominantly lean beef; transferring the material and fluid through a conduit, wherein the conduit comprises more than one outlet located along the length and at a lower section of the conduit; allowing the components that comprise predominantly fat to rise in the fluid as the fluid and material are transferred through the conduit; and removing the components that comprise predominantly lean beef that settle to the bottom of the conduit from at least one outlet at the lower section of the conduit as the fluid and material are transferred through the conduit, wherein components that are removed from the more than one outlet become higher in fat and connective tissue as the fluid progresses through the conduit.

In some embodiments, a method for separating meat components comprises (a) centrifugally spinning a mixture of meat components, a fluid, with or without nanobubbles, including one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, any halogen, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide, or water with nanobubbles, within a centrifuge to separate meat components in concentric zones according to density, wherein denser components accumulate farther away from the axis of rotation and less dense components accumulate closer to the axis of rotation; and (b) transferring denser components towards a first cone-shaped section of the centrifuge via a first screw action and transferring less dense components towards a second cone-shaped section of the centrifuge via a second screw action, wherein gas can accumulate at zones in the proximity of the cone-shaped sections so as to impede the fluid from exiting with the meat components.

In some embodiments, a method for separating fat comprises (a) combining particles comprising fat and lean meat or both fat and lean meat with a fluid; (b) introducing the particles and the fluid into an enclosed separator having one or more inclined or vertical surfaces; (c) separating particles at different elevations of the separator, wherein the particles having a density greater than the fluid will collect at a lower elevation, and the particles that have a density less than the fluid will collect at a relatively higher elevation; and (d) reducing the size of the particles that have a density less than the fluid, and separating lean meat from solid material via a centrifuge.

In some embodiments, a separator manifold comprises (a) a first enclosed conduit disposed at an incline or perpendicular to the manifold; and (b) a second enclosed conduit disposed at an incline or perpendicular to the manifold wherein a lower side of the manifold is joined via a port to an end of the second conduit to allow connective tissue material that settles to the lower side of the manifold to be transferred into the second conduit.

In some embodiments, a method for producing treated meat having a predetermined proportion of water comprises calculating changes of water content in meat during processing of the meat; placing meat in a vessel; introducing at an elevated pressure, a fluid, with or without nanobubbles, comprising an amount of water containing one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, any halogen, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide, or having a pH below 5 into the vessel and in contact with-the surfaces of the meat; providing turbulence in the vessel to expose surfaces of meat to the fluid: wherein said amount of water is a calculated amount of water that is lost during processing to result in a predetermined proportion of water in the meat; and packaging the meat containing a predetermined proportion of water in a container.

In some embodiments, a method for producing treated meat having a predetermined proportion of water in a container comprises determining a proportion of water suitable for a packaged meat; placing meat in a vessel; introducing a bactericide and added water into the vessel, wherein the added water exceeds the predetermined proportion of water suitable for packaged meat; calculating an amount of water that is to be removed in a centrifuge; transferring the meat into a centrifuge and removing water in excess of the predetermined proportion of water in meat to produce treated meat having the predetermined proportion of water suitable for packaged meat; and packaging the meat containing a predetermined amount of water.

In some embodiments, a method of processing perishable products comprises sealing a perishable product in an enclosure; calculating an amount of water to be removed from the perishable product; and transferring the perishable products with an amount of water into a centrifuge to remove said amount of water calculated to be the amount of water that is to be removed to result in a predetermined amount of water in the product when the product is packaged.

In some embodiments, a method for separating beef comprises reducing beef into small beef components; combining the beef components with a liquid in a vessel, wherein the liquid is a blend of carbon dioxide and water or chlorine or chlorine compound and water, wherein the pH of the liquid is reduced; mixing the beef and liquid in the vessel; allowing beef components comprising predominantly fat to rise to the top of the liquid and beef components comprising predominantly lean beef to settle to the bottom of the liquid; removing the beef components comprising predominantly fat from the liquid; and removing the beef components comprising predominantly lean beef from the liquid.

In some embodiments, a method for separating fat from a material comprises reducing a material to smaller material pieces, wherein the material pieces include components comprising predominantly fat and components comprising predominantly lean beef; adjusting the temperature of the material pieces to a range from about 24. degree. F. (-4.4. degree. C.) to about 110. degree. F. (43.3. degree. C); combining the material pieces with a liquid in a vessel, wherein the density of the liquid is greater than or equal to the density of the components comprising predominantly fat and less than or equal to the density of the components comprising predominantly lean beef, wherein the liquid, with or without nanobubbles, includes one or more microbiocidal agents selected from hypochlorous acid, hydrochloric acid, bromine, fluorine, any halogen, sulphuric acid, lactic acid, citric acid, acetic acid, ozone, carbonic acid, carbon dioxide, chlorine, chlorine dioxide, acidified sodium chlorite, a chlorine compound, a chlorine compound and water, an aqueous alkaline solution of sodium hydroxide or calcium hydroxide or any other suitable alkaline solution or acid, or water with carbon dioxide, wherein the pH of the liquid is reduced; allowing the components comprising predominantly fat to rise in the liquid forming a first stratum in the liquid; allowing the components comprising predominantly lean beef to settle in the liquid forming a second stratum in the liquid; and collecting the second stratum comprising components comprising predominantly lean beef.

While the preferred embodiment of the invention has been illustrated and described, it will be appreciated that various changes can be made therein without departing from the spirit and scope of the invention.