SEQUENCING KIT CONTAINING SULFYDRYL BLOCKING REAGENT AND APPLICATION OF SEQUENCING KIT

Title:

SEQUENCING KIT CONTAINING SULFYDRYL BLOCKING REAGENT AND APPLICATION OF SEQUENCING KIT

Document Type and Number:

WIPO Patent Application WO/2023/241701

Kind Code:

A1

Abstract:

Provided are a sequencing kit containing a sulfydryl blocking reagent and an application of the sequencing kit. Also disclosed is a method for reducing a sequencing background signal. The method comprises the following steps: (1) opening a three-dimensional space structure of the enzyme protein, and exposing an active group; (2) blocking the exposed active group, wherein the blocking is irreversible blocking; (3) releasing a fluorescent substance; and (4) cleaning. For the first time, on the basis of a strategy of opening a disulfide bond of the enzyme protein and then performing irreversible blocking so as to better release the wound dyes substance, a background signal in the sequencing is reduced, and a signal-to-noise ratio and sequencing quality are improved, thereby facilitating efficient high-throughput sequencing.

Inventors:

JIA MAN (CN)

Application Number:

PCT/CN2023/100777

Publication Date:

December 21, 2023

Filing Date:

June 16, 2023

Export Citation:

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Assignee:

MGI TECH CO LTD (CN)

International Classes:

C12Q1/68

Foreign References:

CN104483374A	2015-04-01
CN105301119A	2016-02-03
CN112986570A	2021-06-18
CN101042374A	2007-09-26
CN101042376A	2007-09-26
CN101055267A	2007-10-17

Other References:

TURCATTI G, ROMIEU A, FEDURCO M, TAIRI A-P: "A new class of cleavable fluorescent nucleotides: synthesis and optimization as reversible terminators for DNA sequencing by synthesis", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 36, no. 4, 1 March 2008 (2008-03-01), GB , pages E25-1 - E25-13, XP002631196, ISSN: 0305-1048, DOI: 10.1093/NAR/GKN021
YI XU, JUNJIANG LV, WEI FAN, ZHIYU WEN: "Progress of Biochemical Reaction Technologies on Microfluidic Chips", PROGRESS IN CHEMISTRY, vol. 19, no. 5, 24 May 2007 (2007-05-24), pages 820 - 832, XP093118114

Attorney, Agent or Firm:

SHANGHAI BESHINING LAW OFFICE (CN)

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