Title:
SIMPLIFIED MONOCLONAL ANTIBODY QUANTIFICATION METHOD
Document Type and Number:
WIPO Patent Application WO/2019/130536
Kind Code:
A1
Abstract:
The present invention provides a method for detecting a monoclonal antibody in a sample, comprising the steps of: (a) capturing the monoclonal antibody in the sample and immobilizing the monoclonal antibody in a pore in a porous material; (b) bringing the porous material having the monoclonal antibody immobilized therein into contact with a protease-immobilized nano-particle to perform the selective protease digestion of the monoclonal antibody for 30 minutes or longer; and (c) detecting a peptide fragment obtained by the selective protease digestion by liquid chromatography-mass spectrometry (LC-MS), wherein step (b) is performed both under stirring conditions for 10 seconds to 5 minutes after the initiation of the reaction and under standing conditions thereafter. According to the present invention, it becomes possible to simplify a monoclonal antibody detection method employing mass spectrometry, and it also becomes possible to apply the method to the analysis of multiple samples.
Inventors:
SHIMADA TAKASHI (JP)
IWAMOTO NORIKO (JP)
TAKANASHI MEGUMI (JP)
IWAMOTO NORIKO (JP)
TAKANASHI MEGUMI (JP)
Application Number:
PCT/JP2017/047190
Publication Date:
July 04, 2019
Filing Date:
December 28, 2017
Export Citation:
Assignee:
SHIMADZU CORP (JP)
International Classes:
G01N30/06; C12N11/14; G01N1/28; G01N27/62; G01N30/72
Domestic Patent References:
| WO2016143227A1 | 2016-09-15 | |||
| WO2015033479A1 | 2015-03-12 | |||
| WO2016143223A1 | 2016-09-15 | |||
| WO2015033479A1 | 2015-03-12 | |||
| WO2016143223A1 | 2016-09-15 | |||
| WO2016143224A1 | 2016-09-15 | |||
| WO2016143226A1 | 2016-09-15 | |||
| WO2016143227A1 | 2016-09-15 | |||
| WO2016194114A1 | 2016-12-08 | |||
| WO2016143226A1 | 2016-09-15 | |||
| WO2015033479A1 | 2015-03-12 | |||
| WO2016143223A1 | 2016-09-15 | |||
| WO2016143224A1 | 2016-09-15 | |||
| WO2016143227A1 | 2016-09-15 | |||
| WO2016194114A1 | 2016-12-08 |
Foreign References:
| JP2008532019A | 2008-08-14 | |||
| US20090156426A1 | 2009-06-18 |
Other References:
ANALYST., vol. 139, no. 3, 7 February 2014 (2014-02-07), pages 576 - 80
ANAL. METHODS, vol. 21, 2015, pages 9177 - 9183
DRUG METABOLISM AND PHARMACOKINETICS, vol. 31, 2016, pages 46 - 50
BIOANALYSIS, vol. 8, no. 10, 2016, pages 1009 - 20
BIOL PHARM BULL, vol. 39, no. 7, 2016, pages 1187 - 94
J CHROMATOGR B ANALYT TECHNOL BIOMED LIFE SCI, vol. 9-16, 2016, pages 1023 - 1024
CLIN PHARMACOL BIOPHARM, vol. 5, 2016, pages 164
J. PHARM BIOMED ANAL, vol. 145, 2017, pages 33 - 39
"Kyoto Encyclopedia of Genes and Genomes, KEGG"
See also references of EP 3734268A4
ANAL. METHODS, vol. 21, 2015, pages 9177 - 9183
DRUG METABOLISM AND PHARMACOKINETICS, vol. 31, 2016, pages 46 - 50
BIOANALYSIS, vol. 8, no. 10, 2016, pages 1009 - 20
BIOL PHARM BULL, vol. 39, no. 7, 2016, pages 1187 - 94
J CHROMATOGR B ANALYT TECHNOL BIOMED LIFE SCI, vol. 9-16, 2016, pages 1023 - 1024
CLIN PHARMACOL BIOPHARM, vol. 5, 2016, pages 164
J. PHARM BIOMED ANAL, vol. 145, 2017, pages 33 - 39
"Kyoto Encyclopedia of Genes and Genomes, KEGG"
See also references of EP 3734268A4
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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