Title:
SIMULTANEOUS ANAEROBIC PRODUCTION OF ISOAMYL ACETATE AND SUCCINIC ACID
Document Type and Number:
WIPO Patent Application WO2006069174
Kind Code:
A3
Abstract:
In vivo method of producing esters from acetyl CoA, such as isoamyl acetate and succinate, has been developed by producing null mutants in pathways that use acetyl CoA and by overexpressing products that use NADH and in order to maintain the proper redox balance between NADH and NAD. The method is exemplified with null mutations in ldhA, adhE, ackA pta and overexpression of pyruvate carboxylase and alcohol acetyltransferase. This strain produces higher levels of both isoamyl acetate and succinate.
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Inventors:
SAN KA-YIU (US)
SANCHEZ AILEN (US)
BENNETT GEORGE N (US)
DITTRICH CHERYL RENEE (US)
SANCHEZ AILEN (US)
BENNETT GEORGE N (US)
DITTRICH CHERYL RENEE (US)
Application Number:
PCT/US2005/046425
Publication Date:
December 14, 2006
Filing Date:
December 22, 2005
Export Citation:
Assignee:
RICE UNIVERSITY (US)
SAN KA-YIU (US)
SANCHEZ AILEN (US)
BENNETT GEORGE N (US)
DITTRICH CHERYL RENEE (US)
SAN KA-YIU (US)
SANCHEZ AILEN (US)
BENNETT GEORGE N (US)
DITTRICH CHERYL RENEE (US)
International Classes:
C12N1/20; C12P7/46; C12P7/54
Other References:
VADALI ET AL.: "Production of isoamylacetate in ackA-pta and Idh mutants of Escherichia coli with overexpression of yeast ATF2", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 63, 2004, pages 698 - 704, XP003005590
YANG ET AL.: "Redistribution of Metabolic Fluxes in Escherichia coli with Fermentative Lactate dehydrogenase Overexpression and Deletion", METABOLIC ENGINEERING, vol. 1, 1999, pages 141 - 152, XP002227141
SANCHEZ ET AL.: "Efficient Succinic Acid Production from Glucose through Overexpression of Pyruvate Carboxylase in an Escherichia coli Alcohol Dehydrogenase and Lactate Dehydrogenase Mutant", BIOTECHNOL. PROG., vol. 21, 2005, pages 358 - 365, XP002391565
YANG ET AL.: "Redistribution of Metabolic Fluxes in Escherichia coli with Fermentative Lactate dehydrogenase Overexpression and Deletion", METABOLIC ENGINEERING, vol. 1, 1999, pages 141 - 152, XP002227141
SANCHEZ ET AL.: "Efficient Succinic Acid Production from Glucose through Overexpression of Pyruvate Carboxylase in an Escherichia coli Alcohol Dehydrogenase and Lactate Dehydrogenase Mutant", BIOTECHNOL. PROG., vol. 21, 2005, pages 358 - 365, XP002391565
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