SINGLE-PARTICLE ANALYSIS METHOD, AND SYSTEM FOR PERFORMING SAID ANALYSIS

Title:

SINGLE-PARTICLE ANALYSIS METHOD, AND SYSTEM FOR PERFORMING SAID ANALYSIS

Document Type and Number:

WIPO Patent Application WO/2016/182034

Kind Code:

A1

Abstract:

The objective of the present invention is to purify and concentrate differentiated cells derived from ES cells or iPS cells, for example, without damaging the cells. This problem can be solved by means of a device for analyzing and isolating fine particles, the device including a flow-path cartridge according to the present invention, a light radiating means, a detecting means for detecting fine particles of interest, and a force generating means, wherein there are formed in the cartridge: a sample reservoir coupled to a first flow path; a fourth branched flow path and a fifth branched flow path which are coupled to the first flow path; a number 3A reservoir coupled to the fourth branched flow path; a number 3B reservoir coupled to the fifth branched flow path; and a fourth reservoir which accumulates fine particles that have not been split off; and wherein each reservoir has a means for equalizing the air pressure inside the reservoir and the air pressure in an air-pressure control system in each device, and the flows in the flow paths within the cartridge are controlled by controlling the air pressure in each reservoir using the air-pressure control systems in each device.

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Inventors:

TAKEDA KAZUO (JP)
FUJIMURA YUU (JP)
INOU TAKAHIDE (JP)
ISHIGE MASAYUKI (JP)
AKAGI JIN (JP)

Application Number:

PCT/JP2016/064196

Publication Date:

November 17, 2016

Filing Date:

May 12, 2016

Export Citation:

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Assignee:

ON-CHIP BIOTECHNOLOGIES CO LTD (JP)

International Classes:

G01N15/14; C12M1/00; C12Q1/02; G01N21/49; G01N21/64; G01N37/00

Domestic Patent References:

WO2011086990A1

2011-07-21

Foreign References:

US8248604B2	2012-08-21
JPH0251044A	1990-02-21
JP2005524831A	2005-08-18
JP2012165699A	2012-09-06
JP2001120261A	2001-05-08
JP2011512825A	2011-04-28