Title:
SITE-OCCUPYING PRIMER AND REMOVAL METHOD
Document Type and Number:
WIPO Patent Application WO/2023/216030
Kind Code:
A1
Abstract:
A site-occupying primer used in sequencing and a removal method. The site-occupying primer comprises a blocking group at the 3' end. The 3' end sequence of the site-occupying primer is complementary to a part of the sequence of a sequencing template, and the 5' end sequence of the site-occupying primer is not paired with the sequencing template. A method for removing a primer and a related sequencing method. A section of sequence not paired with the template is introduced at the 5' end of the site-occupying primer, and the branch migration action of DNA is used to easily remove the site-occupying primer when the site-occupying
primer is required to be removed.
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Inventors:
LI CHANGYING (CN)
LI JIGUANG (CN)
XU CHONGJUN (CN)
LI JIGUANG (CN)
XU CHONGJUN (CN)
Application Number:
PCT/CN2022/091488
Publication Date:
November 16, 2023
Filing Date:
May 07, 2022
Export Citation:
Assignee:
MGI TECH CO LTD (CN)
International Classes:
C12Q1/6869; C12N15/10; C12Q1/6806
Foreign References:
| CN112063703A | 2020-12-11 | |||
| CN103266103A | 2013-08-28 | |||
| CN112111485A | 2020-12-22 | |||
| US20150259734A1 | 2015-09-17 | |||
| US20100143900A1 | 2010-06-10 | |||
| CN105358710A | 2016-02-24 |
Other References:
SNEZANA DRMANAC, MATTHEW CALLOW, LINSU CHEN, PING ZHOU, LEON ECKHARDT, CHONGJUN XU, MEIHUA GONG, SCOTT GABLENZ, JYOTHI RAJAGOPAL, : "CoolMPSTM: Advanced massively parallel sequencing using antibodies specific to each natural nucleobase", BIORXIV, 20 February 2020 (2020-02-20), XP055732849, Retrieved from the Internet DOI: 10.1101/2020.02.19.953307
Attorney, Agent or Firm:
IP MARCH (CN)
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