Title:
SMALL CAS PROTEINS AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/047624
Kind Code:
A1
Abstract:
Provided is a novel class of RNA base editors comprising a fusion protein that comprises a small Class I CRISPR/Cas effector enzyme, such as Cas5, Cas6, and Csf5 and an RNA based editing functional domain. Also provided are polynucleotides encoding the fusion proteins, and method of using the fusion proteins for RNA base editing.
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Inventors:
YANG HUI (CN)
ZHOU YINGSI (CN)
ZHANG RENXIA (CN)
WANG XING (CN)
ZHOU YINGSI (CN)
ZHANG RENXIA (CN)
WANG XING (CN)
Application Number:
PCT/CN2020/112868
Publication Date:
March 10, 2022
Filing Date:
September 01, 2020
Export Citation:
Assignee:
HUIGENE THERAPEUTICS CO LTD (CN)
International Classes:
C07K19/00; C12N9/78; C12N15/09
Domestic Patent References:
| WO2017215619A1 | 2017-12-21 | |||
| WO2018208998A1 | 2018-11-15 |
Other References:
DATABASE Protein 18 June 2019 (2019-06-18), ANONYMOUS: "CRISPR-associated endoribonuclease Cas6 [Saccharolobus solfataricus]", XP055906559, retrieved from Genbank Database accession no. WP_009992200
DATABASE Protein 2 December 2019 (2019-12-02), ANONYMOUS: "CRISPR-associated endonuclease Cas6 [Methanococcus maripaludis]", XP055906568, retrieved from Genbank Database accession no. WP_011868531
DATABASE Protein 2 June 2019 (2019-06-02), ANONYMOUS: "type I-C CRISPR-associated protein Cas5 [Streptococcus pyogenes]", XP055906579, retrieved from Genbank Database accession no. WP_002989031
DATABASE Protein 2 June 2020 (2020-06-02), ANONYMOUS: "type I-C CRISPR-associated protein Cas5 [Alkalihalobacillus halodurans]", XP055906589, retrieved from Genbank Database accession no. WP_010896518
DATABASE Protein 3 August 2020 (2020-08-03), ANONYMOUS: "MULTISPECIES: CRISPR system precrRNA processing endoribonuclease RAMP protein Cas6 [unclassified Synechococcus]", XP055906595, retrieved from Genbank Database accession no. WP_012308498
DATABASE Protein 3 June 2019 (2019-06-03), ANONYMOUS: "MULTISPECIES: type I-E CRISPR-associated protein Cas6/Cse3/CasE [Enterobacteriaceae]", XP055906601, retrieved from Genbank Database accession no. WP_000281400
DATABASE Protein 15 June 2019 (2019-06-15), ANONYMOUS: "MULTISPECIES: type I-F CRISPR-associated endoribonuclease Cas6/Csy4 [Pseudomonas]", XP055906607, retrieved from Genbank Database accession no. WP_016254196
DATABASE Protein 4 July 2020 (2020-07-04), ANONYMOUS: "CRISPR-associated endoribonuclease Cas6 [Mycobacterium tuberculosis]", XP055906619, retrieved from Genbank Database accession no. WP_003899496
DATABASE Protein 16 June 2019 (2019-06-16), ANONYMOUS: "CRISPR-associated endoribonuclease Cas6 [Pyrococcus furiosus]", XP055906623, retrieved from Genbank Database accession no. WP_011012271
DATABASE Protein 6 December 2017 (2017-12-06), ANONYMOUS: "MULTISPECIES: hypothetical protein [Pseudomonas]", XP055906631, retrieved from Genbank Database accession no. WP_073937547
MICHAL BURMISTRZ, KAMIL KRAKOWSKI, AGATA KRAWCZYK-BALSKA: "RNA-Targeting CRISPR-Cas Systems and Their Applications", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 3, 1 January 2020 (2020-01-01), pages 1122, XP055726418, DOI: 10.3390/ijms21031122
WANG FEI; WANG LIANRONG; ZOU XUAN; DUAN SULING; LI ZHIQIANG; DENG ZIXIN; LUO JIE; LEE SANG YUP; CHEN SHI: "Advances in CRISPR-Cas systems for RNA targeting, tracking and editing", BIOTECHNOLOGY ADVANCES, vol. 37, no. 5, 1 September 2019 (2019-09-01), GB , pages 708 - 729, XP085733157, ISSN: 0734-9750, DOI: 10.1016/j.biotechadv.2019.03.016
DATABASE Protein 2 December 2019 (2019-12-02), ANONYMOUS: "CRISPR-associated endonuclease Cas6 [Methanococcus maripaludis]", XP055906568, retrieved from Genbank Database accession no. WP_011868531
DATABASE Protein 2 June 2019 (2019-06-02), ANONYMOUS: "type I-C CRISPR-associated protein Cas5 [Streptococcus pyogenes]", XP055906579, retrieved from Genbank Database accession no. WP_002989031
DATABASE Protein 2 June 2020 (2020-06-02), ANONYMOUS: "type I-C CRISPR-associated protein Cas5 [Alkalihalobacillus halodurans]", XP055906589, retrieved from Genbank Database accession no. WP_010896518
DATABASE Protein 3 August 2020 (2020-08-03), ANONYMOUS: "MULTISPECIES: CRISPR system precrRNA processing endoribonuclease RAMP protein Cas6 [unclassified Synechococcus]", XP055906595, retrieved from Genbank Database accession no. WP_012308498
DATABASE Protein 3 June 2019 (2019-06-03), ANONYMOUS: "MULTISPECIES: type I-E CRISPR-associated protein Cas6/Cse3/CasE [Enterobacteriaceae]", XP055906601, retrieved from Genbank Database accession no. WP_000281400
DATABASE Protein 15 June 2019 (2019-06-15), ANONYMOUS: "MULTISPECIES: type I-F CRISPR-associated endoribonuclease Cas6/Csy4 [Pseudomonas]", XP055906607, retrieved from Genbank Database accession no. WP_016254196
DATABASE Protein 4 July 2020 (2020-07-04), ANONYMOUS: "CRISPR-associated endoribonuclease Cas6 [Mycobacterium tuberculosis]", XP055906619, retrieved from Genbank Database accession no. WP_003899496
DATABASE Protein 16 June 2019 (2019-06-16), ANONYMOUS: "CRISPR-associated endoribonuclease Cas6 [Pyrococcus furiosus]", XP055906623, retrieved from Genbank Database accession no. WP_011012271
DATABASE Protein 6 December 2017 (2017-12-06), ANONYMOUS: "MULTISPECIES: hypothetical protein [Pseudomonas]", XP055906631, retrieved from Genbank Database accession no. WP_073937547
MICHAL BURMISTRZ, KAMIL KRAKOWSKI, AGATA KRAWCZYK-BALSKA: "RNA-Targeting CRISPR-Cas Systems and Their Applications", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 3, 1 January 2020 (2020-01-01), pages 1122, XP055726418, DOI: 10.3390/ijms21031122
WANG FEI; WANG LIANRONG; ZOU XUAN; DUAN SULING; LI ZHIQIANG; DENG ZIXIN; LUO JIE; LEE SANG YUP; CHEN SHI: "Advances in CRISPR-Cas systems for RNA targeting, tracking and editing", BIOTECHNOLOGY ADVANCES, vol. 37, no. 5, 1 September 2019 (2019-09-01), GB , pages 708 - 729, XP085733157, ISSN: 0734-9750, DOI: 10.1016/j.biotechadv.2019.03.016
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SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
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