Title:
SNARES AND METHODS OF CONTROLLING CYTOKINESIS
Document Type and Number:
WIPO Patent Application WO2003066098
Kind Code:
A3
Abstract:
Two members of the SNARE membrane fusion machinery, syntaxin 2 and endobrevin/VAMP-8, have been found to be important to cytokinesis in mammalian cells. Inhibition of syntaxin 2 and endobrevin/VAMP-8 function by over-expression of non-membrane anchored mutants of these proteins causes failure of cytokinesis leading to the formation of binucleated cells. Time-lapse microscopy shows that only midbody abscission is prevented by over-expression of these non-membrane anchored mutants, and that other cellular events preceeding midbody abscission, such as furrowing, are unaffected.
Inventors:
WEIMBS THOMAS (US)
LOW SENG HUI (US)
LOW SENG HUI (US)
Application Number:
PCT/US2003/004006
Publication Date:
December 11, 2003
Filing Date:
February 10, 2003
Export Citation:
Assignee:
CLEVELAND CLINIC FOUNDATION (US)
WEIMBS THOMAS (US)
LOW SENG HUI (US)
WEIMBS THOMAS (US)
LOW SENG HUI (US)
International Classes:
C07K14/705; A61K38/00; (IPC1-7): C07K14/705; A61K39/395; C12N15/00
Other References:
JANTSCH-PLUNGER VERENA ET AL: "Depletion of syntaxins in the early Caenorhabditis elegans embryo reveals a role for membrane fusion events in cytokinesis", CURRENT BIOLOGY, CURRENT SCIENCE,, GB, vol. 9, no. 14, 15 July 1999 (1999-07-15), pages 738 - 745, XP002211291, ISSN: 0960-9822
CONNER SEAN D ET AL: "Syntaxin is required for cell division.", MOLECULAR BIOLOGY OF THE CELL, vol. 10, no. 8, August 1999 (1999-08-01), pages 2735 - 2743, XP001153903, ISSN: 1059-1524
CHEN YU A ET AL: "Sequential SNARE assembly underlies priming and triggering of exocytosis.", NEURON, vol. 30, no. 1, April 2001 (2001-04-01), pages 161 - 170, XP002251710, ISSN: 0896-6273
LUKOWITZ W ET AL: "Cytokinesis in the Arabidopsis embryo involves the syntaxin-related KNOLLE gene product", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 84, no. 1, 12 January 1996 (1996-01-12), pages 61 - 71, XP002096007, ISSN: 0092-8674
BOWERMAN B ET AL: "CELL DIVISION: PLANT-LIKE PROPERTIES OF ANIMAL CELL CYTOKINESIS", CURRENT BIOLOGY, CURRENT SCIENCE,, GB, vol. 9, no. 17, 9 September 1999 (1999-09-09), pages R658 - R660, XP000982988, ISSN: 0960-9822
LOW S ET AL: "The SNARE membrane fusion proteins syntaxin 2 and endobrevin are required for abscission, the final step of cytokinesis.", MOLECULAR BIOLOGY OF THE CELL, vol. 13, no. Supplement, 20 November 2002 (2002-11-20), 42nd Annual Meeting of the American Society for Cell Biology;San Francisco, CA, USA; December 14-18, 2002, pages 29a, XP009014678, ISSN: 1059-1524
LOW SENG HUI ET AL: "Syntaxin 2 and endobrevin are required for the terminal step of cytokinesis in mammalian cells.", DEVELOPMENTAL CELL, vol. 4, no. 5, 20 May 2003 (2003-05-20), pages 753 - 759, XP009014639, ISSN: 1534-5807
CONNER SEAN D ET AL: "Syntaxin is required for cell division.", MOLECULAR BIOLOGY OF THE CELL, vol. 10, no. 8, August 1999 (1999-08-01), pages 2735 - 2743, XP001153903, ISSN: 1059-1524
CHEN YU A ET AL: "Sequential SNARE assembly underlies priming and triggering of exocytosis.", NEURON, vol. 30, no. 1, April 2001 (2001-04-01), pages 161 - 170, XP002251710, ISSN: 0896-6273
LUKOWITZ W ET AL: "Cytokinesis in the Arabidopsis embryo involves the syntaxin-related KNOLLE gene product", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 84, no. 1, 12 January 1996 (1996-01-12), pages 61 - 71, XP002096007, ISSN: 0092-8674
BOWERMAN B ET AL: "CELL DIVISION: PLANT-LIKE PROPERTIES OF ANIMAL CELL CYTOKINESIS", CURRENT BIOLOGY, CURRENT SCIENCE,, GB, vol. 9, no. 17, 9 September 1999 (1999-09-09), pages R658 - R660, XP000982988, ISSN: 0960-9822
LOW S ET AL: "The SNARE membrane fusion proteins syntaxin 2 and endobrevin are required for abscission, the final step of cytokinesis.", MOLECULAR BIOLOGY OF THE CELL, vol. 13, no. Supplement, 20 November 2002 (2002-11-20), 42nd Annual Meeting of the American Society for Cell Biology;San Francisco, CA, USA; December 14-18, 2002, pages 29a, XP009014678, ISSN: 1059-1524
LOW SENG HUI ET AL: "Syntaxin 2 and endobrevin are required for the terminal step of cytokinesis in mammalian cells.", DEVELOPMENTAL CELL, vol. 4, no. 5, 20 May 2003 (2003-05-20), pages 753 - 759, XP009014639, ISSN: 1534-5807
Download PDF:
Previous Patent: A NOVEL TARGET TO INHIBIT ANGIOGENESIS
Next Patent: IMMUNOCYTOKINE-CONTAINING LYOPHILIZED PREPARATION
Next Patent: IMMUNOCYTOKINE-CONTAINING LYOPHILIZED PREPARATION