Title:
SNP MOLECULAR MARKER FOR IDENTIFICATION OF SEX OF OSTEOGLOSSUM BICIRRHOSUM, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/134451
Kind Code:
A1
Abstract:
Disclosed are an SNP molecular marker suitable for the identification of the sex of Osteoglossum bicirrhosum, and the use thereof. The base sequence of the SNP molecular marker is SEQ ID NO: 1, and the SNP is located at position 85 in SEQ ID NO: 1. A single nucleotide polymorphism (SNP) site that differs between male and female individuals of Osteoglossum bicirrhosum and can characterize male and female sex is obtained by means of genomic high-throughput sequencing and comparison, and an amplification sequence containing the SNP site is obtained by means of amplification in conjunction with primers. On this basis, SNP site information of a sample is obtained by means of PCR amplification and sequencing of a sample under test by using primers, thereby identifying the sex of the sample under test.
Inventors:
MU XIDONG (CN)
LIU YI (CN)
LIU CHAO (CN)
YANG YEXIN (CN)
SONG HONGMEI (CN)
WANG XUEJIE (CN)
XU MENG (CN)
GU DANGEN (CN)
FANG MIAO (CN)
HU YINCHANG (CN)
LIU YI (CN)
LIU CHAO (CN)
YANG YEXIN (CN)
SONG HONGMEI (CN)
WANG XUEJIE (CN)
XU MENG (CN)
GU DANGEN (CN)
FANG MIAO (CN)
HU YINCHANG (CN)
Application Number:
PCT/CN2021/095511
Publication Date:
June 30, 2022
Filing Date:
May 24, 2021
Export Citation:
Assignee:
PEARL RIVER FISHERIES RES INSTITUTE CHINESE ACADEMY OF FISHERY SCIENCES (CN)
International Classes:
C12Q1/68
Foreign References:
| CN108424958A | 2018-08-21 | |||
| CN106119378A | 2016-11-16 |
Other References:
SOUZA, PEREZ, BERTOLLO, OLIVEIRA, LAVOUÉ, GESTICH, RÁB, EZAZ, LIEHR, VIANA, FELDBERG, CIOFFI: "Interspecific Genetic Differences and Historical Demography in South American Arowanas (Osteoglossiformes, Osteoglossidae, Osteoglossum)", GENES, vol. 10, no. 9, pages 693, XP055944621, DOI: 10.3390/genes10090693
Attorney, Agent or Firm:
SCIHEAD IP LAW FIRM (CN)
Download PDF:
Previous Patent: AERIAL ELECTROMAGNETIC FLYING VEHICLE
Next Patent: VOLTAGE STABILIZATION MODULE AND ELECTRONIC APPARATUS
Next Patent: VOLTAGE STABILIZATION MODULE AND ELECTRONIC APPARATUS