Title:
SPLIT INTEIN AND PREPARATION METHOD FOR RECOMBINANT POLYPEPTIDE USING SAME
Document Type and Number:
WIPO Patent Application WO/2021/047558
Kind Code:
A1
Abstract:
Provided is a flanking sequence pair for SspDnaE, SspDnaB, MxeGyrA, MjaTFIIB, PhoVMA, TvoVMA, Gp41-1, Gp41-8, IMPDH-1, or PhoRadA split intein. The flanking sequence pair comprises: flanking sequence a and flanking sequence b, the flanking sequence a being located at N-terminus of the split intein N-terminal protein splicing region (In) and being between N-terminus exon (En) and In, and the flanking sequence b being located at C-terminus of the split intein C-terminal protein splicing region (Ic) and being between Ic and C-terminal exon (Ec).
Inventors:
ZHANG JING (CN)
LUO FANG (CN)
GONG CHENG (CN)
WANG XIN (CN)
FANG LIJUAN (CN)
ZHOU PENGFEI (CN)
LUO FANG (CN)
GONG CHENG (CN)
WANG XIN (CN)
FANG LIJUAN (CN)
ZHOU PENGFEI (CN)
Application Number:
PCT/CN2020/114271
Publication Date:
March 18, 2021
Filing Date:
September 09, 2020
Export Citation:
Assignee:
WUHAN YZY BIOPHARMA CO LTD (CN)
International Classes:
C07K5/06; C07K16/46
Foreign References:
| CN106397598A | 2017-02-15 | |||
| CN108884154A | 2018-11-23 | |||
| CN104053779A | 2014-09-17 | |||
| CN107075491A | 2017-08-18 | |||
| CN101899489A | 2010-12-01 |
Other References:
DASSA B, LONDON N, STODDARD B L, SCHUELER-FURMAN O, PIETROKOVSKI S: "Fractured Genes: A Novel Genomic Arrangement Involving New Split Inteins and a New Homing Endonuclease Family", NUCLEIC ACIDS RESEARCH, vol. 37, no. 8, 5 March 2009 (2009-03-05), pages 2560 - 2573, XP002758805, ISSN: 1362-4962, DOI: 20201210131853Y
Attorney, Agent or Firm:
BEIJING C&S LAW FIRM (CN)
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