Title:
SQUALENE-HOPENE CYCLASE GENE OF ACETIC ACID BACTERIUM, ACETIC ACID BACTERIUM BRED WITH THE USE OF THE GENE, AND PROCESS FOR PRODUCING VINEGAR USING THE ACETIC ACID BACTERIUM
Document Type and Number:
WIPO Patent Application WO/2003/078622
Kind Code:
A1
Abstract:
A novel acetic acid bacterium-origin gene participating in acetic acid-tolerance; a method of improving the acetic acid-tolerance of a microorganism, in particular, an acetic acid bacterium, using this gene; and a process of efficiently producing a vinegar having an elevated acetic acid concentration with the use of the acetic acid bacterium having the thus improved acetic acid tolerance. From a chromosomal DNA library of an acetic acid bacterium, a gene enabling the growth of the acetic acid bacterium in a medium containing acetic acid at such a high concentration as not allowing the growth in usual is obtained. By using this method, a novel gene participating in acetic acid-tolerance is cloned from an acetic acid bacterium for practical use belonging to the genus Gluconacetobacter. A transformant constructed by transferring this gene into an acetic acid bacterium has a remarkably improved acetic acid-tolerance. When this transformant is cultured under aeration in the presence of ethanol, the growth induction period is shortened and the growth speed and the acid-forming speed are elevated. Moreover, the final carryover acetic acid level thereof can be remarkably elevated thereby.
Inventors:
GOTO HIDETSUGU (JP)
NAKANO SHIGERU (JP)
NAKANO SHIGERU (JP)
Application Number:
PCT/JP2003/002731
Publication Date:
September 25, 2003
Filing Date:
March 07, 2003
Export Citation:
Assignee:
MITSUKAN GROUP CORP (JP)
GOTO HIDETSUGU (JP)
NAKANO SHIGERU (JP)
GOTO HIDETSUGU (JP)
NAKANO SHIGERU (JP)
International Classes:
C12J1/04; C12N1/21; C12N9/88; C12P7/54; (IPC1-7): C12N15/09; C07K14/195; C12J1/04; C12N1/21
Foreign References:
| JPH03219878A | 1991-09-27 | |||
| EP0332120A2 | 1989-09-13 | |||
| JPS609489A | 1985-01-18 | |||
| JPS609488A | 1985-01-18 | |||
| JPH05199887A | 1993-08-10 | |||
| JPS60180581A | 1985-09-14 |
Other References:
FREIBERG C. ET AL.: "Molecular basis of symbiosis between rhizobium and legumes", NATURE, vol. 387, no. 6631, 1997, pages 394 - 401, XP002969316
REIPEN I.G. ET AL.: "Zymomonas mobilis squalene-hopene cyclase gene (shc): cloning, DNA sequence analysis and expression in escherichia coli", MICROBIOLOGY, vol. 141, no. 1, 1995, pages 155 - 161, XP002969317
TIPPELT A. ET AL.: "Squalene-hopene cyclase from methylococcus capsulatus (Bath): a bacterium producing hopanoids and steroids", BIOCHEM. BIOPHYS. ACTA, vol. 1391, no. 2, 1998, pages 223 - 232, XP002969318
FUKAYA M. ET AL.: "The aarC gene responsible for acetic acid assimilation confers acetic acid resistance on acetobacter aceti.", J. FERMENT. BIOENG., vol. 76, no. 4, 1993, pages 270 - 275, XP002969319
SCHULLER G. ET AL.: "Gluconacetobacter entanii sp. nov., isolated from submerged high-acid industrial vinegar fermentations", INT. J. SYST. EVOL. MICROBIOL., vol. 50, no. 6, 2000, pages 2013 - 2020, XP002969320
REIPEN I.G. ET AL.: "Zymomonas mobilis squalene-hopene cyclase gene (shc): cloning, DNA sequence analysis and expression in escherichia coli", MICROBIOLOGY, vol. 141, no. 1, 1995, pages 155 - 161, XP002969317
TIPPELT A. ET AL.: "Squalene-hopene cyclase from methylococcus capsulatus (Bath): a bacterium producing hopanoids and steroids", BIOCHEM. BIOPHYS. ACTA, vol. 1391, no. 2, 1998, pages 223 - 232, XP002969318
FUKAYA M. ET AL.: "The aarC gene responsible for acetic acid assimilation confers acetic acid resistance on acetobacter aceti.", J. FERMENT. BIOENG., vol. 76, no. 4, 1993, pages 270 - 275, XP002969319
SCHULLER G. ET AL.: "Gluconacetobacter entanii sp. nov., isolated from submerged high-acid industrial vinegar fermentations", INT. J. SYST. EVOL. MICROBIOL., vol. 50, no. 6, 2000, pages 2013 - 2020, XP002969320
Attorney, Agent or Firm:
Toda, Chikao (Hogaku Building 503 1-19-14, Toranomo, Minato-ku Tokyo, JP)
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