Title:
STRAIN FOR PRODUCING NATTOKINASE AND PRODUCTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2021/000247
Kind Code:
A1
Abstract:
Disclosed are a strain for producing nattokinase and a production method therefor. In particular, the present invention involves a new strain capable of producing nattokinase, i.e. Bacillus subtilis natto ST-1086, deposited at the China General Microbiological Culture Collection Center under CGMCC No. 17895. The present invention further involves a method for producing a nattokinase product by means of using the new strain CGMCC No. 17895 of the present application, wherein the resulting nattokinase product can be used as a drug for dissolving thrombi. The present invention further involves the use of the nattokinase product of the present application for preparing a composition for dissolving thrombi and in a method for treating thrombi.
Inventors:
CHEN JIEPENG (CN)
DUAN LILI (CN)
HONG LIN (CN)
JI YEYU (CN)
CHEN HONGRUI (CN)
CAI CHUNLI (CN)
HU LIUSONG (CN)
XU ZHIKAI (CN)
CHEN YUFAN (CN)
DUAN LILI (CN)
HONG LIN (CN)
JI YEYU (CN)
CHEN HONGRUI (CN)
CAI CHUNLI (CN)
HU LIUSONG (CN)
XU ZHIKAI (CN)
CHEN YUFAN (CN)
Application Number:
PCT/CN2019/094334
Publication Date:
January 07, 2021
Filing Date:
July 02, 2019
Export Citation:
Assignee:
SUNGEN BIOSCIENCE CO LTD (CN)
International Classes:
C12N1/20; A61K38/48; A61P7/02; C12N9/56; C12R1/125
Foreign References:
| CN110408558A | 2019-11-05 | |||
| CN101560478A | 2009-10-21 | |||
| CN107828685A | 2018-03-23 | |||
| CN101979531A | 2011-02-23 | |||
| CN103695359A | 2014-04-02 | |||
| CN109589405A | 2019-04-09 |
Other References:
MAN LI-LI; XIANG DIAN-JUN; ZHANG CHUN-LAN: "Strain Screening from Traditional Fermented Soybean Foods and Induction of Nattokinase Production in Bacillus subtilis MX-6", PROBIOTICS AND ANTIMICROBIAL PROTEINS, vol. 11, no. 1, 6 February 2018 (2018-02-06), pages 283 - 294, XP037245644, ISSN: 1867-1306, DOI: 10.1007/s12602-017-9382-7
ZHONGMEI LIU, ZHENG WENHUI, GE CHUNLEI, CUI WENJING, ZHOU LI, ZHOU ZHEMIN: "High-level extracellular production of recombinant nattokinase in Bacillus subtilis WB800 by multiple tandem promoters", BMC MICROBIOLOGY, vol. 19, no. 1, 89, 7 May 2019 (2019-05-07), pages 1 - 14, XP055771316, DOI: 10.1186/s12866-019-1461-3
XIONG QIANG ET AL.: "Study on Liquid Fermentation Conditions of Nattokinase", CHINESE JOURNAL OF BIOPROCESS ENGINEERING, vol. 10, no. 4, 2012, pages 26 - 29
See also references of EP 3892715A4
ZHONGMEI LIU, ZHENG WENHUI, GE CHUNLEI, CUI WENJING, ZHOU LI, ZHOU ZHEMIN: "High-level extracellular production of recombinant nattokinase in Bacillus subtilis WB800 by multiple tandem promoters", BMC MICROBIOLOGY, vol. 19, no. 1, 89, 7 May 2019 (2019-05-07), pages 1 - 14, XP055771316, DOI: 10.1186/s12866-019-1461-3
XIONG QIANG ET AL.: "Study on Liquid Fermentation Conditions of Nattokinase", CHINESE JOURNAL OF BIOPROCESS ENGINEERING, vol. 10, no. 4, 2012, pages 26 - 29
See also references of EP 3892715A4
Attorney, Agent or Firm:
AFD CHINA INTELLECTUAL PROPERTY LAW OFFICE (CN)
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