Title:
STRAIN FOR SYNTHESIZING 2'-FUCOSYLLACTOSE BY TAKING GLUCOSE AS CARBON SOURCE, CONSTRUCTION METHOD THEREFOR, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/216685
Kind Code:
A1
Abstract:
The present invention belongs to the technical field of bioengineering. Provided are a strain for synthesizing 2'-fucosyllactose by taking glucose as a carbon source, a construction method therefor, and use thereof. The strain is obtained by co-transforming recombinant plasmids pACYCDuet-J23115-LacY and ptrc99a-ManCB-Gmdfcl-FucT into Escherichia coli. The provided strain is used to synthesize 2'-fucosyllactose by taking glucose as the carbon source, with a yield of 3.2 g/L.
Inventors:
XIA HONGZHI (CN)
XU ZHENG (CN)
CHU CHENGHUI (CN)
LI GUYUE (CN)
NIU KUN (CN)
LI JIANGBO (CN)
SUN YI (CN)
YANG CHENLIANG (CN)
ZHANG JIANHONG (CN)
ZHU YULEI (CN)
XU ZHENG (CN)
CHU CHENGHUI (CN)
LI GUYUE (CN)
NIU KUN (CN)
LI JIANGBO (CN)
SUN YI (CN)
YANG CHENLIANG (CN)
ZHANG JIANHONG (CN)
ZHU YULEI (CN)
Application Number:
PCT/CN2023/079163
Publication Date:
November 16, 2023
Filing Date:
March 02, 2023
Export Citation:
Assignee:
RICHEN BIOENGINEERING CO LTD (CN)
International Classes:
C12N1/21; C12N15/52; C12N15/54; C12N15/70; C12P19/00; C12R1/19
Foreign References:
| CN115011535A | 2022-09-06 | |||
| CN109943514A | 2019-06-28 | |||
| CN107849577A | 2018-03-27 | |||
| CN112342176A | 2021-02-09 | |||
| CN112501106A | 2021-03-16 | |||
| CN105722991A | 2016-06-29 | |||
| CN106190937A | 2016-12-07 | |||
| CN111471637A | 2020-07-31 | |||
| CN113462629A | 2021-10-01 | |||
| CN112625987A | 2021-04-09 | |||
| US20210115452A1 | 2021-04-22 |
Attorney, Agent or Firm:
BEIJING HESHINIP INTELLECTUAL PROPERTY OFFICE (CN)
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