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Title:
STRAINS OF AGROBACTERIUM MODIFIED TO INCREASE PLANT TRANSFORMATION FREQUENCY
Document Type and Number:
WIPO Patent Application WO/2012/016222
Kind Code:
A3
Abstract:
Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.

Inventors:
MERLO DONALD J (US)
RUSSELL SEAN M (US)
RETALLACK DIANE (US)
WOOSLEY AARON (US)
MEADE TOM (US)
NARVA KENNETH E (US)
Application Number:
PCT/US2011/046028
Publication Date:
June 21, 2012
Filing Date:
July 29, 2011
Export Citation:
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Assignee:
DOW AGROSCIENCES LLC (US)
MERLO DONALD J (US)
RUSSELL SEAN M (US)
RETALLACK DIANE (US)
WOOSLEY AARON (US)
MEADE TOM (US)
NARVA KENNETH E (US)
International Classes:
A01H5/00; C12N1/21; C12N15/82; C12N15/84
Foreign References:
US5731179A1998-03-24
US20060212973A12006-09-21
US20060174372A12006-08-03
US20090075358A12009-03-19
Other References:
ROGER HELLENS ET AL.: "Technical Focus : A guide to Agrobacterium binary Ti vectors", TRENDS IN PLANT SCIENCE, vol. 5, no. 10, 2000, pages 446 - 451, XP027089993
Attorney, Agent or Firm:
CATAXINOS, Edgar R. (PO Box 2550Salt Lake City, Utah, US)
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