The present compounds show a high and selective binding affinity to the histamine H3 recep- tor indicating histamine H3 receptor antagonistic, inverse agonistic or agonistic activity. As a result, the compounds are useful for the treatment of diseases and disorders related to the histamine H3 receptor.

BACKGROUND OF THE INVENTION The existence of the histamine H3 receptor has been known for several years and the receptor is of current interest for the development of new medicaments (see eg Stark, H.; Schlicker, E.; Schunack, W. , Drugs Fut. 1996, 21, 507-520; Leurs, R.; Timmerman, H.; Vollinga, R. C., Progress in Drug Research 1995,45, 107-165). Recently, the human hista- mine H3 receptor has been cloned, cf Lovenberg, T. W. et al, Molecular Pharmacology, June 1999,55, 1101-1107. The histamine H3 receptor is a presynaptic autoreceptor located both in the central and the peripheral nervous system, the skin and in organs such as the lung, the intestine, probably the spleen and the gastrointestinal tract. Recent evidence suggests that the H3 receptor shows intrinsic, constitutive activity, in vitro as well as in vivo (ie it is active in the absence of an agonist; see eg Morisset et al., Nature 2000,408, 860-864). Compounds acting as inverse agonists can inhibit this activity. The histamine H3 receptor has been dem- onstrated to regulate the release of histamine and also of other neurotransmitters such as serotonin and acetylcholine. A histamine H3 receptor antagonist or inverse agonist would therefore be expected to increase the release of these neurotransmitters in the brain. A his- tamine H3 receptor agonist, on the contrary, leads to an inhibition of the biosynthesis of his- tamine and an inhibition of the release of histamine and also of other neurotransmitters such as serotonin and acetylcholine. These findings suggest that histamine H3 receptor agonists, inverse agonists and antagonists could be important mediators of neuronal activity. Accord- ingly, the histamine H3 receptor is an important target for new therapeutics.

Piperidines similar to the compounds of the present invention have previously been prepared, and their biological properties have been investigated, cf Chemical Abstracts 1966, 46341; Arch. Pharm. 1979,312 (8), 670-681; Meanwell, N. A. et al., J. Med. Chem. 1992,35 ; 14; 2688-2696; Drugs Des. Discovery 1995, 12 (3), 249-258; US 4,943, 573; WO 01/21206 ;

WO 99/48491 ; WO 97/43282; WO 97/43292; WO 84/03089; WO 84/01576; WO 83/03410; Weinstock, L. T. et al., J. Pharm. Sci. 1981,70 (8), 956-959; and US 3,337, 551.

However, these references neither disclose nor suggest that these substituted piperidines may have a histamine H3 receptor antagonistic or agonistic activity.

Several publications disclose the preparation and use of histamine H3 agonists and antagonists. Most of these are imidazole derivatives (see eg Stark et al., Drugs of the Future 1996, 21, 507-520; Tozer, Kalinddjian, Expert Opinion on Therapeutic Patents 2000, 10, 1045-1055). However, recently some imidazole-free ligands of the rat histamine H3 receptor have been described. Thus, Walczynski et al. (Arch. Pharm. Pharm. Med. Chem. 1999,332, 389-398), Linney et al. (J. Med. Chem. 2000,43, 2362-2370), Ganellin et al. (Arch. Pharm.

Pharm. Med. Chem. 1998, 331, 395-404), Walczynski et al. (II Farmaco 1999,54, 684-694), Kalindjian et al. (WO 99/42458), Schwartz et al. (EP 0 978 512), and Ludwig et al. (WO 97/17345) disclose cyclic amines having rat histamine H3 receptor agonistic or antagonistic activity. However, the structures of these amines are quite different from that of the present compounds. Thus, none of the amines disclosed in these publications contain a piperidine structure, as is the case in the present compounds.

In view of the art's interest in histamine H3 receptor agonists, inverse agonists and antagonists, novel compounds which interact with the histamine H3 receptor would be a highly desirable contribution to the art. The present invention provides such a contribution to the art being based on the finding that a novel class of substituted piperidines has a high and specific affinity to the histamine H3 receptor.

Due to their interaction with the histamine H3 receptor, the present compounds are useful in the treatment of a wide range of conditions and disorders in which an interaction with the histamine H3 receptor is beneficial. Thus, the compounds may find use eg in the treatment of diseases of the central nervous system, the peripheral nervous system, the car- diovascular system, the pulmonary system, the gastrointestinal system and the endocrino- logical system.

DEFINITIONS In the structural formulae given herein and throughout the present specification, the following terms have the indicated meaning: The term"halogen"means F, Cl, Br or 1.

The term"C16-alkyl"as used herein represents a branched or straight hydrocarbon group having from 1 to 6 carbon atoms. Typical C, _6-alkyl groups include, but are not limited

to, methyl, ethyl, n-propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, isopentyl, hexyl, isohexyl and the like.

The term"C2-r,-alkenyl"as used herein represents a branched or straight hydrocar- bon group having from 2 to 6 carbon atoms and at least one double bond. Examples of such groups include, but are not limited to, ethenyl, 1-propenyl, 2-propenyl, allyl, iso-propenyl, 1, 3-butadienyl, 1-butenyl, 2-butenyl, 1-pentenyl, 2-pentenyl, 1-hexenyl, 2-hexenyl and the like.

The term"C2-6-alkynyl"as used herein represents a branched or straight hydrocar- bon group having from 2 to 6 carbon atoms and at least one triple bond. Examples of such groups include, but are not limited to, ethynyl, 1-propynyl, 2-propynyl, 1-butynyl, 2-butynyl, 1- pentynyl, 2-pentynyl, 1-hexynyl, 2-hexynyl and the like.

The term"C1 6-alkoxy"as used herein, alone or in combination, refers to the radical- O-C1-6-alkyl wherein C1-6-alkyl is as defined above. Representative examples are methoxy, ethoxy, n-propoxy, isopropoxy, butoxy, sec-butoxy, teff-butoxy, pentoxy, isopentoxy, hexoxy, isohexoxy and the like.

The term"C-6-alkylthio"as used herein, alone or in combination, refers to the radi- cal-S-C1 6-alkyl wherein C1-6-alkyl is as defined above. Representative examples are methyl- thio, ethylthio, isopropylthio, n-propylthio, butylthio, pentylthio and the like.

The term"C1 6-alkylsulfonyl"as used herein, alone or in combination, refers to the radical-S (=O) 2-C1 6-alkyl wherein C1 6-alkyl is as defined above. Representative examples are methylsulfonyl, ethylsulfonyl, isopropylsulfonyl, n-propylsulfonyl, butylsulfonyl, pentylsulfonyl and the like.

The term"C1 7-alkanoyl"as used herein, alone or in combination, refers to the radi- cal-C (=O) H or-C (=O) C1 6-alkyl wherein C1 6-alkyl is as defined above. Representative examples are formyl, acetyl, propionyl, butanoyl, pentanol, hexanoyl, heptanoyl and the like.

The term"C3_8-cycloalkyl"as used herein represents a monocyclic, carbocyclic group having from from 3 to 8 carbon atoms. Representative examples are cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl and the like.

The term"C5-8-CYCloalkenyl"as used herein represents a monocyclic, carbocyclic, non-aromatic group having from 5 to 8 carbon atoms and at least one double bond. Repre- sentative examples are cyclopentenyl, cyclohexenyl, cyclohepentyl, cyclooctenyl, and the like.

The term"aryl"as used herein is intended to include carbocyclic aromatic ring systems such as phenyl, biphenylyl, naphthyl, anthracenyl, phenanthrenyl, fluorenyl, indenyl, pentalenyl, azulenyl and the like. Aryl is also intended to include the partially hydrogenated derivatives of

the carbocyclic systems enumerated above. Non-limiting examples of such partially hydro- genated derivatives are 1,2, 3, 4-tetrahydronaphthyl, 1, 4-dihydronaphthyl and the like.

The term"aryloxy"as used herein refers to the radical-O-aryl where aryl is as de- fined above. Non-limiting examples are phenoxy, naphthoxy, anthracenyloxy, phenantreny- loxy, fluorenyloxy, indenyloxy and the like.

The term"heteroaryl"as used herein is intended to include heterocyclic aromatic ring systems containing one or more heteroatoms selected from nitrogen, oxygen and sulfur such as furyl, thienyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, isoxazolyl, isothiazolyl, 1,2, 3- triazolyl, 1,2, 4-triazolyl, pyranyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, 1,2, 3-triazinyl, 1,2, 4-triazinyl, 1,3, 5-triazinyl, 1,2, 3-oxadiazolyl, 1,2, 4-oxadiazolyl, 1,2, 5-oxadiazolyl, 1,3, 4- oxadiazolyl, 1,2, 3-thiadiazolyl, 1,2, 4-thiadiazolyl, 1,2, 5-thiadiazolyl, 1,3, 4-thiadiazolyl, tetrazolyl, thiadiazinyl, indolyl, isoindolyl, benzofuryl, benzothienyl, indazolyl, benzimidazolyl, benzthia- zolyl, benzisothiazolyl, benzoxazolyl, benzisoxazolyl, purinyl, quinazolinyl, quinolizinyl, quino- linyl, isoquinolinyl, quinoxalinyl, naphthyridinyl, pteridinyl, carbazolyl, azepinyl, diazepinyl, acridinyl and the like. Heteroaryl is also intended to include the partially hydrogenated deriva- tives of the heterocyclic systems enumerated above. Non-limiting examples of such partially hydrogenated derivatives are 2, 3-dihydrobenzofuranyl, pyrrolinyl, pyrazolinyl, indolinyl, oxa- zolidinyl, oxazolinyl, oxazepinyl and the like.

As used herein, the phrase"4 to 7 membered, saturated or unsaturated heterocyclic ring"is intended to include heterocyclic rings which are saturated or contain one or two dou- ble bonds.

Certain of the above defined terms may occur more than once in the structural for- mulae, and upon such occurrence each term shall be defined independently of the other.

The term"optionally substituted"as used herein means that the group in question is either unsubstituted or substituted with one or more of the substituents specified. When the group in question is substituted with more than one substituent the substituents may be the same or different.

"Aryl-C1 6-alkyl","aryl-C1 6-alkoxy"etc. mean C1 6-alkyl or C1 6-alkoxy as defined above, substituted by aryl as defined above, for example: The term"treatment"as used herein means the management and care of a patient for the purpose of combating a disease, disorder or condition. The term is intended to include

the delaying of the progression of the disease, disorder or condition, the alleviation or relief of symptoms and complications, and/or the cure or elimination of the disease, disorder or condi- tion. The patient to be treated is preferably a mammal, in particular a human being.

DESCRIPTION OF THE INVENTION The invention relates to a compound of the general formula (I) : wherein m is 1, 2 or 3, q is 0,1 or 2, <BR> <BR> <BR> <BR> <BR> <BR> X is-CH2- (CH2) -,- (CH2) n-CH=CH- (CH2) p,-CH2- (CH2) n-O- (CH2) p-,<BR> <BR> <BR> <BR> <BR> -CH2-(CH2) n-C (=O)-(CH2) p-,-CH2-(CH2) n-S-(CH2) p-,-CH2-(CH2) n-S (=O)-(CH2) p-or -CH2-(CH2)n-S(=O)2-(CH2)p-, n and p are independently 0,1, 2,3 or 4, R'and R2 are independently hydrogen, methyl or trifluoromethyl, Y is (a) aryl or heteroaryl, which may optionally be substituted with one or more substituents se- lected from halogen, nitro, cyano, hydroxy, C1-7-alkanoyl, C1-6-alkylthio, C1-6-alkylsulfonyl, C1-6-alkyl, C1-6-alkoxy, C3-8-cycloalkyl, trifluoromethyl, trifluoromethoxy, -NR3r4 and -O (C=O) NR R,

wherein R3 and R4 independently are hydrogen, C1-6-alkyl, C3-8-cycloalkyl, C1 7-alkanoyl or aryl, or R3 and R4 together with the nitrogen atom to which they are attached form a 4 to 7 membered, saturated or unsaturated ring, # or wherein two substituents in adjacent positions form a radical-O-(CH2)1-3-O-, # aryl, aryl-C1 6-alkyl and aryl-C1 6-alkoxy, wherein the ring moieties optionally may be substituted with one or more substituents selected from o halogen, nitro, cyano, hydroxy, C1-7-alkanoyl, C1-6-alkylthio, C1-6-alkylsulfonyl, C1-6-alkyl, C1-6-alkoxy, C3-8-cycloalkyl, trifluoromethyl, trifluoromethoxy, -NR5R6 and-0 (C=O) NR5R6, wherein R5 and R6 independently are hydrogen, C1-6-alkyl, C3-8-cycloalkyl, C1 7-alkanoyl or aryl, or R5 and R6 together with the nitrogen atom to which they are attached form a 4 to 7 membered, saturated or unsaturated ring, o or wherein two substituents in adjacent positions form a radical-O-(CH2)1-3-O (b) C3 8-cycloalkyl or C5 8-cycloalkenyl, which may optionally be substituted with one or more substituents selected from # C1-6-alkyl, C1-6-alkoxy, C1-6-alkylthio, cyano, trifluoromethyl, trifluoromethoxy and halo- gen, # aryl and aryloxy, wherein the ring moieties optionally may be substituted with one or more substituents selected from o halogen, nitro, cyano, hydroxy, C1-7-alkanoyl, C1-6-alkylthio, C1-6-alkylsulfonyl, C1-6-alkyl, C1-6-alkoxy, C3-8-cycloalkyl, trifluoromethyl, trifluoromethoxy, -NR7R8 and-0 (C=O) NR7R8, wherein R7 and R8 independently are hydrogen, C1-6-alkyl, C3-8-cycloalkyl, CI-7-alkanoyl or aryl, or R7 and R8 together with the nitrogen atom to which they are attached form a 4 to 7 membered, saturated or unsaturated ring, o or wherein two substituents in adjacent positions form a radical-O-(CH2)1-3-O-

(c) C1 6-alkyl, C2 6-alkenyl and C2 6-alkynyl, which may optionally be substituted with one or more substituents selected from halogen, nitro, cyano, hydroxy, C1-7-alkanoyl, C1-6-alkylthio, C1-6-alkylsulfonyl, C1-6-alkoxy, C3-8-cycloalkyl, trifluoromethyl, trifluoromethoxy, -NR9R10 and -O(C=O)NR9R10, wherein R9 and Rlo independently are hydrogen, C1-6-alkyl, C3-8-cycloalkyl, C1 7-alkanoyl or aryl, or R9 and R10 together with the nitrogen atom to which they are attached form a 4 to 7 membered, saturated or unsaturated ring, aryl, aryl-C1-6-alkyl and aryl-C1 6-alkoxy, wherein the ring moieties optionally may be substituted with one or more substituents selected from o halogen, nitro, cyano, hydroxy, C1-7-alkanoyl, C1-6-alkylthio, C1-6-alkylsulfonyl, C1-6-alkyl, C1-6-alkoxy, C3-8-cycloalkyl, trifluoromethyl, trifluoromethoxy, -NR11R12 and -O(C=O)NR11R12, wherein R11 and R12 independently are hydrogen, C1 6-alkyl, C3 8-cycloalkyl, C1-7-alkanoyl or aryl, or R"and R12 together with the nitrogen atom to which they are attached form a 4 to 7 membered, saturated or unsaturated ring, o or wherein two substituents in adjacent positions form a radical -O-(CH2)1-3-O- with the proviso that the compound must not be

as well as any diastereomer or enantiomer or tautomeric form thereof including mixtures of these or a pharmaceutically acceptable salt thereof.

In one embodiment, m is 1.

In another embodiment, m is 2.

In still another embodiment, q is 1.

In yet another embodiment, R'and R2 are both hydrogen.

In a further embodiment, X is-CH2-(CH2) n-,-(CH2) n-CH=CH-(CH2) p-,-CH2-(CH2) n-O-(CH2) p-, -CH2-(CH2) n-C (=O)-(CH2) p-, or-CH2-(CH2) n-S-(CH2) p-, wherein n and p are as defined for formula (I).

In still a further embodiment, X is -CH2-(CH2)n-, -CH=CH-, -CH2-(CH2)n-O-, -CH2-(CH2) n-C (=O)-,-CH2-S- (CH2) p-, wherein n is 0,1, 2 or 3, and p is 0 or 1, such as-CH2-, -(CH2) 2-,-(CH2) 3-,-(CH2) 4-,-CH=CH-,-CH2-O-,-(CH2) 3-O-,-(CH2) 2-C (=O)-,-CH2-S-CH2-or - CH2-S-.

In another embodiment, Y is C1 6-alkyl, C3 8-cycloalkyl, aryl or heteroaryl, which may option- ally be substituted as defined for formula (I).

In yet another embodiment, Y is C1 6-alkyl, cyclohexyl, phenyl, naphthyl, pyridyl, benzoxa- zolyl, imidazolyl or benzothiophenyl, which may optionally be substituted as defined for for- mula (I).

In a further embodiment, Y is phenyl or naphthyl, which may optionally be substituted with one or more substituents selected from trifluoromethyl, trifluoromethoxy, halogen, nitro, C1-6-alkyl, -NR3R4, -O-(C=O)NR3R4 and C1-7-alkanoyl, wherein R3 and R4 independently are hydrogen, C1-6-alkyl, C3-8-cycloalkyl, C1-7-alkanoyl or aryl, or R3 and R4 together with the nitrogen atom to which they are attached form a 4 to 7 membered, saturated or unsaturated ring, # aryl-C1 6-alkoxy, aryl-C1 6-alkyl and aryl, which may optionally be substituted with halogen or C1-6-alkyl, 'or wherein two substituents in adjacent positions form a radical -O-(CH2)1-3-O-.

In still another embodiment, Y is phenyl or naphthyl, which may optionally be substituted with one or more substituents selected from trifluoromethyl, trifluoromethoxy, halogen, nitro, C1-6-alkyl, -NR3R4, -O(C=O)NR3R4 and C1 7-alkanoyl, wherein R3 and R4 independently are hydrogen or C1 6-alkyl, or R3 and R4 together with the nitrogen atom to which they are attached form a 4 to 7 membered, saturated or unsaturated ring, # phenyl-C1-6-alkoxy, phenyl-C1-6-alkyl and phenyl, which may optionally be substituted with halogen or C1-6-alkyl,

or wherein two substituents in adjacent positions form a radical-O-(CH2) 1 3-O-.

The compounds of the present invention may be chiral, and it is intended that any enantiomers, as separated, pure or partially purified enantiomers or racemic mixtures thereof are included within the scope of the invention.

Furthermore, when a double bond or a fully or partially saturated ring system or more than one center of asymmetry or a bond with restricted rotatability is present in the molecule, diastereomers may be formed. It is intended that any diastereomers, as separated, pure or partially purified diastereomers or mixtures thereof are included within the scope of the invention.

Furthermore, some of the compounds of the present invention may exist in different tautomeric forms and it is intended that any tautomeric forms, which the compounds are able to form, are included within the scope of the present invention.

The present invention also encompasses pharmaceutically acceptable salts of the present compounds. Such salts include pharmaceutically acceptable acid addition salts, pharmaceutical acceptable metal salts, ammonium and alkylated ammonium salts. Acid addition salts include salts of inorganic acids as well as organic acids. Representative exam- ples of suitable inorganic acids include hydrochloric, hydrobromic, hydroiodic, phosphoric, sulfuric, nitric acids and the like. Representative examples of suitable organic acids include formic, acetic, trichloroacetic, trifluoroacetic, propionic, benzoic, cinnamic, citric, fumaric, gly- colic, lactic, maleic, malic, malonic, mandelic, oxalic, picric, pyruvic, salicylic, succinic, methanesulfonic, ethanesulfonic, tartaric, ascorbic, pamoic, bismethylene salicylic, ethanedi- sulfonic, gluconic, citraconic, aspartic, stearic, palmitic, EDTA, glycolic, p-aminobenzoic, glu- tamic, benzenesulfonic, p-toluenesulfonic acids and the like. Further examples of pharma- ceutically acceptable inorganic or organic acid addition salts include the pharmaceutically acceptable salts listed in J. Pharm. Sci. 1977,66, 2, which is incorporated herein by refer- ence. Examples of metal salts include lithium, sodium, potassium, magnesium salts and the like. Examples of ammonium and alkylated ammonium salts include ammonium, methylam- monium, dimethylammonium, trimethylammonium, ethylammonium, hydroxyethylammonium, diethylammonium, butylammonium, tetramethylammonium salts and the like.

Also intended as pharmaceutically acceptable acid addition salts are the hydrates, which the present compounds are able to form.

The acid addition salts may be obtained as the direct products of compound synthe- sis. In the alternative, the free base may be dissolved in a suitable solvent containing the ap-

propriate acid, and the salt isolated by evaporating the solvent or otherwise separating the salt and solvent.

The compounds of the present invention may form solvates with standard low mo- lecular weight solvents using methods well known to the person skilled in the art. Such sol- vates are also contemplated as being within the scope of the present invention.

The invention also encompasses prodrugs of the present compounds, which on ad- ministration undergo chemical conversion by metabolic processes before becoming active pharmacological substances. In general, such prodrugs will be functional derivatives of the present compounds, which are readily convertible in vivo into the required compound of the formula (I). Conventional procedures for the selection and preparation of suitable prodrug derivatives are described, for example, in"Design of Prodrugs", ed. H. Bundgaard, Elsevier, 1985.

The invention also encompasses active metabolites of the present compounds.

The compounds of the present invention interact with the histamine H3 receptor and are accordingly useful for the treatment of a wide variety of conditions and disorders in which histamine H3 receptor interactions are beneficial.

Accordingly, in another aspect the present invention relates to a compound of the general formula (I) as well as any diastereomer or enantiomer or tautomeric form thereof in- cluding mixtures of these or a pharmaceutically acceptable salt thereof for use as a pharma- ceutical composition.

The invention also relates to pharmaceutical compositions comprising, as an active ingredient, at least one compound of the formula (I) or any diastereomer or enantiomer or tautomeric form thereof including mixtures of these or a pharmaceutical acceptable salt thereof together with one or more pharmaceutical acceptable carriers or diluents.

Furthermore, the invention relates to the use of a compound of the general formula (I) : wherein

m is 1, 2 or 3, q is 0,1 or 2, X iS-CH2- (CH2) n-,- (CH2) n-CH=CH- (CH2) p-,-CH2- (CH2) n-O- (CH2) p-, -CH2-(CH2)n-C(=O)- (CH2) p,-CH2- (CH2) n-S- (CH2) p-,-CH2- (CH2) n-S (=O)- (CH2) p- or -CH2-(CH2)n-S(=O)2- (CH2) p-, n and p are independently 0,1, 2,3 or 4, R'and R2 are independently hydrogen, methyl or trifluoromethyl, Y is (a) aryl or heteroaryl, which may optionally be substituted with one or more substituents se- lected from halogen, nitro, cyano, hydroxy, C1-7-alkanoyl, C1-6-alkylthio, C1-6-alkylsulfonyl, C1-6-alkyl, C1-6-alkoxy, C3-8-cycloalkyl, trifluoromethyl, trifluoromethoxy, -NR3R4 and -O(C=O)NR3R4, wherein R3 and R4 independently are hydrogen, C1-6-alkyl, C3-8-cycloalkyl, C1 7-alkanoyl or aryl, or R3 and R4 together with the nitrogen atom to which they are attached form a 4 to 7 membered, saturated or unsaturated ring, 'or wherein two substituents in adjacent positions form a radical -O-(CH2)1-3-O-, # aryl, aryl-C1-6-alkyl and aryl-C1 6-alkoxy, wherein the ring moieties optionally may be substituted with one or more substituents selected from o halogen, nitro, cyano, hydroxy, C1-7-alkanoyl, C1-6-alkylthio, C1-6-alkylsulfonyl, C1-6-alkyl, C1-6-alkoxy, C3-8-cycloalkyl, trifluoromethyl, trifluoromethoxy, -NR5R6 and-0 (C=O) NR5R6, wherein R5 and R6 independently are hydrogen, C1-6-alkyl, C3-8-cycloalkyl, CI-7-alkanoyl or aryl, or R5 and R6 together with the nitrogen atom to which they are attached form a 4 to 7 membered, saturated or unsaturated ring, o or wherein two substituents in adjacent positions form a radical -O-(CH2)1-3-O

(b) C3 8-cycloalkyl or C5 8-cycloalkenyl, which may optionally be substituted with one or more substituents selected from C1-6-alkyl, C1-6-alkoxy, C1-6-alkylthio, cyano, trifluoromethyl, trifluoromethoxy and halo- gen, aryl and aryloxy, wherein the ring moieties optionally may be substituted with one or more substituents selected from o halogen, nitro, cyano, hydroxy, C1-7-alkanoyl, C1-6-alkylthio, C1-6-alkylsulfonyl, C1-6-alkyl, C1-6-alkoxy, C3-8-cycloalkyl, trifluoromethyl, trifluoromethoxy, -NR7R8 and-0 (C=O) NR7R8, wherein R7 and R8 independently are hydrogen, C1-6-alkyl, C3-8-cycloalkyl, C1-7-alkanoyl or aryl, or R7 and R8 together with the nitrogen atom to which they are attached form a 4 to 7 membered, saturated or unsaturated ring, o or wherein two substituents in adjacent positions form a radical -O-(CH2)1-3-O- (c) C1-6-alkyl, C2-6-alkenyl and C2-6-alkynyl, which may optionally be substituted with one or more substituents selected from halogen, nitro, cyano, hydroxy, C1-7-alkanoyl, C1-6-alkylthio, C1-6-alkylsulfonyl, C1-6-alkoxy, C3-8-cycloalkyl, trifluoromethyl, trifluoromethoxy, -NR9R10 and -O (C=O) NR R, wherein R9 and Rlo independently are hydrogen, C1-6-alkyl, C3-8-cycloalkyl, CI-7-alkanoyl or aryl, or R9 and Rlo together with the nitrogen atom to which they are attached form a 4 to 7 membered, saturated or unsaturated ring, # aryl, aryl-C1 6-alkyl and aryl-C1 6-alkoxy, wherein the ring moieties optionally may be substituted with one or more substituents selected from o halogen, nitro, cyano, hydroxy, CI-7-alkanoyl, Cl-6-alkylthio, CI-6-alkylsulfonyl, C1-6-alkyl, C1-6-alkoxy, C3-8-cycloalkyl, trifluoromethyl, trifluoromethoxy, -NR11R12 and -O(C=O)NR11R12, wherein R11 and R12 independently are hydrogen, C1-6-alkyl, C3-8-cycloalkyl, C1 7-alkanoyl or aryl, or R"and R 12 together with the nitrogen atom to which they are attached form a 4 to 7 membered, saturated or unsaturated ring,

o or wherein two substituents in adjacent positions form a radical-0- (CH2) 1-3-0- as well as any diastereomer or enantiomer or tautomeric form thereof including mixtures of these or a pharmaceutical acceptable salt thereof for the preparation of a pharmaceutical composition for the treatment of disorders and diseases related to the histamine H3 receptor.

In still another aspect, the invention relates to a method for the treatment of dis- eases and disorders related to the histamine H3 receptor, the method comprising administer- ing to a subject in need thereof an effective amount of a compound of the formula (I') or any diastereomer or enantiomer or tautomeric form thereof including mixtures of these or a pharmaceutical acceptable salt thereof or a pharmaceutical composition comprising the same.

In one aspect the invention relates to compounds with histamine H3 receptor an- tagonistic activity or inverse agonistic activity which may accordingly be useful in the treat- ment of a wide range of conditions and disorders in which histamine H3 receptor blockade is beneficial.

In another aspect the invention relates to compounds with histamine H3 receptor agonistic activity and which may accordingly be useful in the treatment of a wide range of conditions and disorders in which histamine H3 receptor activation is beneficial.

In one embodiment, the present compounds are used for the preparation of a phar- maceutical composition for the reduction of weight.

In another embodiment, the present compounds are used for the preparation of a pharmaceutical composition for the treatment of overweight or obesity.

In yet another embodiment, the present compounds are used for the preparation of a pharmaceutical composition for the suppression of appetite or satiety induction.

In still a further embodiment, the present compounds are used for the preparation of a pharmaceutical composition for the prevention and/or treatment of disorders and diseases related to overweight or obesity such as atherosclerosis, hypertension, IGT (impaired glu- cose tolerance), diabetes, especially Type 2 diabetes (NIDDM (non-insulin dependent diabe- tes mellitus)), dyslipidemia, coronary heart disease, gallbladder disease, osteoarthritis and various types of cancer such as endometrial, breast, prostate and colon cancers.

In yet a further embodiment, the present compounds are used for the preparation of a pharmaceutical composition for the prevention and/or treatment of eating disorders such as bulimia and binge eating.

In another embodiment, the present compounds are used for the preparation of a pharmaceutical composition for the treatment of IGT.

In still another further embodiment, the present compounds are used for the prepa- ration of a pharmaceutical composition for the treatment of Type 2 diabetes.

In yet another embodiment, the present compounds are used for the preparation of a pharmaceutical composition for the delaying or prevention of the progression from IGT to Type 2 diabetes.

In a further embodiment, the present compounds are used for the preparation of a pharmaceutical composition for the delaying or prevention of the progression from non- insulin requiring Type 2 diabetes to insulin requiring Type 2 diabetes.

The compounds of the present invention may also be used for the treatment of air- way disorders such as asthma, as anti-diarrhoeals and for the modulation of gastric acid se- cretion.

Furthermore, the compounds of the present invention may be used for the treatment of diseases associated with the regulation of sleep and wakefulness and for the treatment of narcolepsy and attention deficit disorders.

Moreover, the compounds of the invention may be used as CNS stimulants or as sedatives.

The present compounds may also be used for the treatment of conditions asso- ciated with epilepsy. Additionally, the present compounds may be used for the treatment of motion sickness and vertigo. Furthermore, they may be useful as regulators of hypothalamo- hypophyseal secretion, antidepressants, modulators of cerebral circulation, and in the treat- ment of irritable bowel syndrome.

Further, the compounds of the present invention may be used for the treatment of dementia and Alzheimer's disease.

The compounds of the present invention may also be useful for the treatment of al- lergic rhinitis, ulcer or anorexia.

The compounds of the present invention may furthermore be useful for the treat- ment of migraine, see R. L. McLeod et al., The Journal of Pharmacology and Experimental Therapeutics 287 (1998), 43-50, and for the treatment of myocardial infarction, see C. J.

Mackins and R. Levi, Expert Opinion on Investigational Drugs 9 (2000), 2537-2542.

In a further aspect of the invention, treatment of a patient with the present com- pounds is combined with diet and/or exercise.

In a further aspect of the invention the present compounds may be administered in combination with one or more further pharmacologically active substances in any suitable ra- tios. Such further active agents may be selected from antiobesity agents, antidiabetics, anti- hypertensive agents, agents for the treatment of complications resulting from or associated

with diabetes and agents for the treatment of complications and disorders resulting from or associated with obesity.

Thus, in a further aspect of the invention the present compounds may be adminis- tered in combination with one or more antiobesity agents or appetite regulating agents.

Such agents may be selected from the group consisting of CART (cocaine am- phetamine regulated transcript) agonists, NPY (neuropeptide Y) antagonists, MC4 (melano- cortin 4) agonists, MC3 (melanocortin 3) agonists, orexin antagonists, TNF (tumor necrosis factor) agonists, CRF (corticotropin releasing factor) agonists, CRF BP (corticotropin releas- ing factor binding protein) antagonists, urocortin agonists, (33 adrenergic agonists such as CL-316243, AJ-9677, GW-0604, LY362884, LY377267 orAZ-40140, MSH (melanocyte- stimulating hormone) agonists, MCH (melanocyte-concentrating hormone) antagonists, CCK (cholecystokinin) agonists, serotonin re-uptake inhibitors such as fluoxetine, seroxat or cita- lopram, serotonin and noradrenaline re-uptake inhibitors, mixed serotonin and noradrenergic compounds, 5HT (serotonin) agonists, bombesin agonists, galanin antagonists, growth hor- mone, growth factors such as prolactin or placenta lactogen, growth hormone releasing compounds, TRH (thyreotropin releasing hormone) agonists, UCP 2 or 3 (uncoupling protein 2 or 3) modulators, leptin agonists, DA agonists (bromocriptin, doprexin), lipase/amylase in- hibitors, PPAR (peroxisome proliferator-activated receptor) modulators, RXR (retinoid X re- ceptor) modulators, TR (3 agonists, AGRP (Agouti related protein) inhibitors, opioid antago- nists (such as naltrexone), exendin-4, GLP-1 and ciliary neurotrophic factor.

In one embodiment of the invention the antiobesity agent is leptin.

In another embodiment the antiobesity agent is dexamphetamine or amphetamine.

In another embodiment the antiobesity agent is fenfluramine or dexfenfluramine.

In still another embodiment the antiobesity agent is sibutramine.

In a further embodiment the antiobesity agent is orlistat.

In another embodiment the antiobesity agent is mazindol or phentermine.

In still another embodiment the antiobesity agent is phendimetrazine, diethylpropion, fluoxetine, bupropion, topiramate or ecopipam.

Suitable antidiabetic agents include insulin, insulin analogues and derivatives such as those disclosed in EP 792 290 (Novo Nordisk A/S), eg N8B29-tetradecanoyl des (B30) human insulin, EP 214 826 and EP 705 275 (Novo Nordisk A/S), eg Asp328 human insulin, US 5,504, 188 (Eli Lilly), eg LysB28 ProB29 human insulin, EP 368 187 (Aventis), eg Lantus, which are all incorporated herein by reference, GLP-1 derivatives such as those disclosed in WO 98/08871 (Novo Nordisk A/S), which is incorporated herein by reference, as well as orally active hypoglycaemic agents.

The orally active hypoglycaemic agents preferably comprise imidazolines, sulpho- nylureas, biguanides, meglitinides, oxadiazolidinediones, thiazolidinediones, insulin sensitiz- ers, a-glucosidase inhibitors, agents acting on the ATP-dependent potassium channel of the a-cells eg potassium channel openers such as those disclosed in WO 97/26265, WO 99/03861 and WO 00/37474 (Novo Nordisk A/S) which are incorporated herein by refer- ence, or mitiglinide, or a potassium channel blocker, such as BTS-67582, nateglinide, gluca- gon antagonists such as those disclosed in WO 99/01423 and WO 00/39088 (Novo Nordisk A/S and Agouron Pharmaceuticals, Inc.), which are incorporated herein by reference, GLP-1 agonists such as those disclosed in WO 00/42026 (Novo Nordisk A/S and Agouron Pharma- ceuticals, Inc.), which are incorporated herein by reference, DPP-IV (dipeptidyl peptidase-IV) inhibitors, PTPase (protein tyrosine phosphatase) inhibitors, inhibitors of hepatic enzymes involved in stimulation of gluconeogenesis and/or glycogenolysis, glucose uptake modula- tors, GSK-3 (glycogen synthase kinase-3) inhibitors, compounds modifying the lipid metabo- lism such as antilipidemic agents, compounds lowering food intake, PPAR (peroxisome pro- liferator-activated receptor) and RXR (retinoid X receptor) agonists, such as ALRT-268, LG-1268 or LG-1069.

In one embodiment of the invention the present compounds are administered in combination with insulin.

In a further embodiment of the invention the present compounds are administered in combination with a sulphonylurea eg tolbutamide, chlorpropamide, tolazamide, glibencla- mide, glipizide, glimepiride, glicazide or glyburide.

In another embodiment of the invention the present compounds are administered in combination with a biguanide eg metformin.

In yet another embodiment of the invention the present compounds are adminis- tered in combination with a meglitinide eg repaglinide or nateglinide.

In still another embodiment of the invention the present compounds are adminis- tered in combination with a thiazolidinedione insulin sensitizer eg troglitazone, ciglitazone, pioglitazone, rosiglitazone, isaglitazone, darglitazone, englitazone, CS-011/CI-1037 or T 174 or the compounds disclosed in WO 97/41097, WO 97/41119, WO 97/41120, WO 00/41121 and WO 98/45292 (Dr. Reddy's Research Foundation), which are incorporated herein by ref- erence.

In still another embodiment of the invention the present compounds may be admin- istered in combination with an insulin sensitizer eg such as GI 262570, YM-440, MCC-555, JTT-501, AR-H039242, KRP-297, GW-409544, CRE-16336, AR-H049020, LY510929, MBX-102, CLX-0940, GW-501516 or the compounds disclosed in WO 99/19313,

WO 00/50414, WO 00/63191, WO 00/63192, WO 00/63193 (Dr. Reddy's Research Founda- tion) and WO 00/23425, WO 00/23415, WO 00/23451, WO 00/23445, WO 00/23417, WO 00/23416, WO 00/63153, WO 00/63196, WO 00/63209, WO 00/63190 and WO 00/63189 (Novo Nordisk A/S), which are incorporated herein by reference.

In a further embodiment of the invention the present compounds are administered in combination with an a-glucosidase inhibitor eg voglibose, emiglitate, miglitol or acarbose.

In another embodiment of the invention the present compounds are administered in combination with an agent acting on the ATP-dependent potassium channel of the (3-cells eg tolbutamide, glibenclamide, glipizide, glicazide, BTS-67582 or repaglinide.

In yet another embodiment of the invention the present compounds may be adminis- tered in combination with nateglinide.

In still another embodiment of the invention the present compounds are adminis- tered in combination with an antilipidemic agent eg cholestyramine, colestipol, clofibrate, gemfibrozil, lovastatin, pravastatin, simvastatin, probucol or dextrothyroxine.

In another aspect of the invention, the present compounds are administered in com- bination with more than one of the above-mentioned compounds eg in combination with met- formin and a sulphonylurea such as glyburide ; a sulphonylurea and acarbose; nateglinide and mefformin ; acarbose and mefformin ; a sulfonylurea, metformin and troglitazone ; insulin and a sulfonylurea ; insulin and metformin ; insulin, mefformin and a sulfonylurea ; insulin and troglitazone ; insulin and lovastatin ; etc.

Furthermore, the present compounds may be administered in combination with one or more antihypertensive agents. Examples of antihypertensive agents are ß-blockers such as alprenolol, atenolol, timolol, pindolol, propranolol and metoprolol, ACE (angiotensin con- verting enzyme) inhibitors such as benazepril, captopril, enalapril, fosinopril, lisinopril, quinapril and ramipril, calcium channel blockers such as nifedipine, felodipine, nicardipine, isradipine, nimodipine, diltiazem and verapamil, and a-blockers such as doxazosin, urapidil, prazosin and terazosin. Further reference can be made to Remington: The Science and Practice of Pharmacy, 19th Edition, Gennaro, Ed. , Mack Publishing Co. , Easton, PA, 1995.

It should be understood that any suitable combination of the compounds according to the invention with diet and/or exercise, one or more of the above-mentioned compounds and optionally one or more other active substances are considered to be within the scope of the present invention.

PHARMACEUTICAL COMPOSITIONS The compounds of the invention may be administered alone or in combination with pharmaceutical acceptable carriers or excipients, in either single or multiple doses. The pharmaceutical compositions according to the invention may be formulated with phar- maceutically acceptable carriers or diluents as well as any other known adjuvants and ex- cipients in accordance with conventional techniques such as those disclosed in Remington: The Science and Practice of Pharmacy, 19th Edition, Gennaro, Ed. , Mack Publishing Co., Easton, PA, 1995.

The pharmaceutical compositions may be specifically formulated for administration by any suitable route such as the oral, rectal, nasal, pulmonary, topical (including buccal and sublingual), transdermal, intracisternal, intraperitoneal, vaginal and parenteral (including sub- cutaneous, intramuscular, intrathecal, intravenous and intradermal) route, the oral route be- ing preferred. It will be appreciated that the preferred route will depend on the general condi- tion and age of the subject to be treated, the nature of the condition to be treated and the ac- tive ingredient chosen.

Pharmaceutical compositions for oral administration include solid dosage forms such as capsules, tablets, dragees, pills, lozenges, powders and granules. Where appropri- ate, they can be prepared with coatings such as enteric coatings or they can be formulated so as to provide controlled release of the active ingredient such as sustained or prolonged release according to methods well known in the art.

Liquid dosage forms for oral administration include solutions, emulsions, suspen- sions, syrups and elixirs.

Pharmaceutical compositions for parenteral administration include sterile aqueous and non-aqueous injectable solutions, dispersions, suspensions or emulsions as well as ster- ile powders to be reconstituted in sterile injectable solutions or dispersions prior to use. De- pot injectable formulations are also contemplated as being within the scope of the present invention.

Other suitable administration forms include suppositories, sprays, ointments, cremes, gels, inhalants, dermal patches, implants etc.

A typical oral dosage is in the range of from about 0.001 to about 100 mg/kg body weight per day, preferably from about 0.01 to about 50 mg/kg body weight per day, and more preferred from about 0.05 to about 10 mg/kg body weight per day administered in one or more dosages such as 1 to 3 dosages. The exact dosage will depend upon the frequency and mode of administration, the sex, age, weight and general condition of the subject

treated, the nature and severity of the condition treated and any concomitant diseases to be treated and other factors evident to those skilled in the art.

The formulations may conveniently be presented in unit dosage form by methods known to those skilled in the art. A typical unit dosage form for oral administration one or more times per day such as 1 to 3 times per day may contain of from 0.05 to about 1000 mg, preferably from about 0.1 to about 500 mg, and more preferred from about 0.5 mg to about 200 mg.

For parenteral routes, such as intravenous, intrathecal, intramuscular and similar administration, typically doses are in the order of about half the dose employed for oral ad- ministration.

The compounds of this invention are generally utilized as the free substance or as a pharmaceutically acceptable salt thereof. One example is an acid addition salt of a com- pound having the utility of a free base. When a compound of the formula (I) contains a free base such salts are prepared in a conventional manner by treating a solution or suspension of a free base of the formula (I) with a chemical equivalent of a pharmaceutically acceptable acid, for example, inorganic and organic acids. Representative examples are mentioned above. Physiologically acceptable salts of a compound with a hydroxy group include the an- ion of said compound in combination with a suitable cation such as sodium or ammonium ion.

For parenteral administration, solutions of the novel compounds of the formula (I) in sterile aqueous solution, aqueous propylene glycol or sesame or peanut oil may be em- ployed. Such aqueous solutions should be suitable buffered if necessary and the liquid dilu- ent first rendered isotonic with sufficient saline or glucose. The aqueous solutions are par- ticularly suitable for intravenous, intramuscular, subcutaneous and intraperitoneal administra- tion. The sterile aqueous media employed are all readily available by standard techniques known to those skilled in the art.

Suitable pharmaceutical carriers include inert solid diluents or fillers, sterile aqueous solution and various organic solvents. Examples of solid carriers are lactose, terra alba, su- crose, cyclodextrin, talc, gelatine, agar, pectin, acacia, magnesium stearate, stearic acid or lower alkyl ethers of cellulose. Examples of liquid carriers are syrup, peanut oil, olive oil, phospholipids, fatty acids, fatty acid amines, polyoxyethylene or water. Similarly, the carrier or diluent may include any sustained release material known in the art, such as glyceryl monostearate or glyceryl distearate, alone or mixed with a wax. The pharmaceutical compo- sitions formed by combining the novel compounds of the formula (I) and the pharmaceutically acceptable carriers are then readily administered in a variety of dosage forms suitable for the

disclosed routes of administration. The formulations may conveniently be presented in unit dosage form by methods known in the art of pharmacy.

Formulations of the present invention suitable for oral administration may be pre- sented as discrete units such as capsules or tablets, each containing a predetermined amount of the active ingredient, and which may include a suitable excipient. These formula- tions may be in the form of powder or granules, as a solution or suspension in an aqueous or non-aqueous liquid, or as an oil-in-water or water-in-oil liquid emulsion.

If a solid carrier is used for oral administration, the preparation may be tablette, placed in a hard gelatine capsule in powder or pellet form or it can be in the form of a troche or lozenge. The amount of solid carrier will vary widely but will usually be from about 25 mg to about 1 g. If a liquid carrier is used, the preparation may be in the form of a syrup, emul- sion, soft gelatine capsule or sterile injectable liquid such as an aqueous or non-aqueous liq- uid suspension or solution.

A typical tablet, which may be prepared by conventional tabletting techniques, may contain: Core: Active compound (as free compound or salt thereof) 5.0 mg Lactosum Ph. Eur. 67.8 mg Cellulose, microcryst. (Avicel) 31.4 mg Amberlite@ IRP88* 1.0 mg Magnesii stearas Ph. Eur. q. s.

Coating: Hydroxypropyl methylcellulose approx. 9 mg Mywacett 9-40 T** approx. 0.9 mg * Polacrillin potassium NF, tablet disintegrant, Rohm and Haas.

** Acylated monoglyceride used as plasticizer for film coating.

If desired, the pharmaceutical composition of the invention may comprise the com- pound of the formula (I) in combination with further pharmacologically active substances such as those described in the foregoing.

EXAMPLES In the examples the following terms are intended to have the following, general meanings: DCM: dichloromethane, methylenechloride DIC : diisopropylcarbodiimide DMF: N, N-dimethyl formamide DMSO: dimethyl sulphoxide THF: tetrahydrofuran NMR spectra were recorded on Bruker 300 MHz and 400 MHz instruments. HPLC- MS was performed on a Perkin Elmer instrument (API 100).

HPLC-systems from Merck-Hitachi (Hibar RT 250-4, Lichrosorb RP 18,5. 0 um, 4.0 x 250 mm, gradient elution, 20% to 80% acetonitrile in water within 30 min, 1.0 ml/min, detection at 254 nm) and Waters (Symmetry, C18,3. 5 um, 3.0 x 150 mm, gradient elution, 5% to 90% acetonitrile in water within 15 min, 1.0 ml/min, detection at 214 nm) were used.

Furthermore, where stated the following HPLC method h8 was used: The reverse phase analysis was performed using UV detections at 214,254, 276 and 301 nm on a 218TP54 4.6 mm x 150 mm C-18 silica column, which was eluted at 1 ml/min at 42 °C. The column was equilibrated with 5% acetonitrile, 85% water and 10% of a solution of 0.5% trifluoroacetic acid in water and eluted by a linear gradient from 5% acetoni- trile, 85% water and 10% of a solution of 0.5% trifluoroacetic acid to 90% acetonitrile and 10% of a solution of 0.5% trifluoroacetic acid over 15 min.

Typical procedure Example 10 2-Biphenyl-4-yl-1- (4-pyrrolidin-1-ylpiperidin-1-yl) ethanone hydrochloride

To the polymeric nitrophenol (1.5 g, approx. 1 mmol) was added a filtered solution of 4-biphenylylacetic acid (1.10 g, 5.18 mmol) in a mixture of 1, 2-dichloropropane (18 ml) and DMF (2 ml), followed by the addition of a solution of DIC (0.63 g, 4.99 mmol) in 1, 2-dichloro- propane (5 ml). The mixture was shaken at room temperature for 15 hours, filtered, and the polymer was extensively washed with DCM, DMF, and 1, 2-dichloropropane. To the polymer was added 1, 2-dichloropropane (5 ml) and a solution of 4- (1-pyrrolidinyl) piperidine (0.123 g, 0.80 mmol) in 1, 2-dichloropropane (10 ml). The resulting mixture was shaken at room tem- perature for 21 hours and then at 60 °C for one hour, filtered, and the polymer was carefully washed with DCM and methanol. The combined filtrates were concentrated to yield the crude product, which was mixed with ethanol and 1 molar aqueous hydrochloric acid, concentrated, and the residual hydrochloride was recrystallized from ethanol. 90 mg (29%) of the title com- pound was obtained.

1 H NMR (400 MHz, DMSO): 5 1. 54 (m, 2H), 1.80-2. 12 (m, 6H), 2.20 (m, 1H), 2.59 (m, 1H), 2.88 (m, 1H), 3.01 (m, 2H), 3.42 (m, 2H), 3.78 (m, 2H), 4.11 (m, 1H), 4.51 (m, 1H), 7.28-7. 38 (m, 3H), 7.46 (t, J = 8 Hz, 2H), 7.60-7. 68 (m, 4H), 10.9 (br s, 1 H) ; HPLC-MS: m/z 349 (MH+); Rt: 4.3 min.

Using this methodology the following compounds were prepared: No Structure Name Found MH+ 1- [1, 4'] Bipiperidinyl-1'-yl-3- [4- (4- 423 fluorobenzyloxy) phenyl] propenone o oui zon GON 2 F F 1-[1, 4'] Bipiperidinyl-1'-yl-3- (4-trifluoro- 369 methylphenyl) propan-1-one ZU 0 1- [1, 4'] Bipiperidinyl-1'-yl-2- (2- 333 methoxyphenoxy) ethanone Nj : : D , o eN 0 1- [1, 4'] Bipiperidinyl-1'-yl-3- (2- 329 0 0 CH3 methoxyphenyl) propenone 3 j0 GN 5 1- [1, 4'] Bipiperidinyl-1'-yl-2- (2-chloro- 337 phenoxy) ethanone 0 cl Nô Zon 6 0 1- [1, 4'] Bipiperidinyl-1'-yl-3-naphtha- 349 len-1-ylpropenone zu N G 7 0 1- [1, 4'] Bipiperidinyl-1'-yl-3- (5-bromo- 421 NN o^CH 2-ethoxyphenyl) propenone ber 0 Br 8 2-Biphenyl-4-yl-1- [1, 4'] bipiperidinyl-1'-363 CNX WJ ylethanone N C, N G 9 CH 1- [1, 4'] Bipiperidinyl-1'-yl-3- (3- 329 1 3 methoxyphenyl) propenone 0 N 0 o-2-Biphenyl-4-yi-1- (4-pyrrolidin- 1-yl-349 piperidin-1-yl) ethanone N 0 11 O CH 4-(4-Methoxyphenyl)-1-(4-pyrrolidin-331 1-ylpiperidin-1-yl) butan-1-one GN 0'" GN 12 2-(2, 4-Dichloro-5-388 methylphenylsulfanyl)-1- (4-pyrrolidin- 1-ylpiperidin-1-yl) ethanone 0 cri CNA S N'cri 13 ol CH3 1- (3-Fluoro-4-methoxyphenyl)-4- (4- 363 O VF pyrrolidin-1-ylpiperidin-1-yl) butane- u'F 1, 4-dione ci go 14 H3CsN, CH3 3-(4-Dimethylamino-phenyl)-1-(4-328 N pyrrolidin-1-ylpiperidin-1-yl) propenone o cl ZON 15 2- (2-Benzyloxyphenyl)-l- (4-pyrrolidin- 379 1-ylpiperidin-1-yl) ethanone r N N O 16 Hscl0 3- (3, 4-Dimethoxyphenyl)-l- (4-pyrro- 347 lidin-1-ylpiperidin-1-yl) propan-1-one 0 0 nu : D U CN 17 4-(2, 4-Dichlorophenoxy)-1-(4-386 pyrrolidin-1-ylpiperidin-1-yl) butan-1- ci one ° r zu 0 18 3- (2-Methoxyphenyl)-l- (4-pyrrolidin- 317 1-ylpiperidin-1-yl) propan-1-one zu ZON N 19 Cl 4-(4-Chloro-2-methylphenoxy)-1-(4-365 pyrrolidin-1-ylpiperidin-1-yl) butan-1- Chine ° r zu go 20 F 2- (4-Fluorophenylsulfanyl)-1- (4- 323 pyrrolidin-1-ylpiperidin-1-yl) ethanone 0 NUS Zon 21 0 F F 3- (4-Fluoro-3-trifluoromethylphenyl)- 371 1- (4-pyrrolidin-1-ylpiperidin-1-yl)- propenone 22 F F 1- (4-Pyrrolidin-1-ylpiperidin-1-yl)-2- (3- 357 trifluoromethoxyphenyl) ethanone F N/J\ j 0 23 F 2- (4-Fluorophenoxy)-1- (4-pyrrolidin-1- 307 ylpiperidin-1-yl) ethanone 0 N0 CN-04 24 + 2-(2, 3-Dichlorophenoxy)-1-(4-358 pyrrolidin-1-ylpiperidin-1-yl) ethanone oa 0 ci Zon 25 H3Cso 2-(4-Methoxyphenoxy)-1-(4-pyrrolidin-319 1-ylpiperidin-1-yl) ethanone 0 N. a 26 0, 1- (4-Pyrrolidin-1-ylpiperidin-1-yl)-2- (4- 357 o r trifluoromethoxyphenyl) ethanone F 0 u N 27 3-Benzo [1, 3] dioxol-5-yl-1- (4- 331 pyrrolidin-1-ylpiperidin-1-yl) propan-1- N'o o one ¢J 28 0 2- (Naphthalen-2-yloxy)-1- (4- 339 pyrrolidin-1-ylpiperidin-1-yl) ethanone r i N 29 H3Cso 1-(4-Pyrrolidin-1-ylpiperidin-1-yl)-3-377 H3C SO CH3 (3, 4, 5-trimethoxyphenyl) propan-1-one 3 I 3 o j0 c 30 o, CH3 3-(2, 4-Dimethoxyphenyl)-1-(4-345 pyrrolidin-1-ylpiperidin-1-yl) propenone 0 . CH3 JE 0 31 1-Biphenyl-4-yl-4- (4-pyrrolidin-1-391 'CN S J ylpiperidin-1-yi) butane-1, 4-dione (, N CN 32 2-(Naphthalen-2-ylsulfanyl)-1-(4-355 pyrrolidin-1-ylpiperidin-1-yl) ethanone 0 Nus zon 33 CH3 3- (3, 5-Dimethoxyphenyl)-1- (4-pyrro- 345 lidin-1-ylpiperidin-1-yl) propenone 0 N G 34 CH3 3- (2, 3-Dimethoxyphenyl)-1- (4-pyrro- 345 lidin-1-ylpiperidin-1-yl) propenone CN N/ 0 J 35 o, CH3 4-(3, 4-Dimethoxyphenyl)-1-(4-pyrro-361 o ol CH3 lidin-1-ylpiperidin-1-yl) butan-1-one ZON C ? U 36 C H 3 2- (2, 3-Dimethylphenoxy)-l- (4-pyrro- 317 lidin-1-ylpiperidin-1-yl) ethanone ? T"CH, neo 0 37 O 2-(8-Chloronaphthalen-1-ylsulfany,)-390 g 1- (4-pyrrolidin-1-ylpiperidin-1-yl)- I IN ethanone 0" 38 o 2- (Naphthalen-1-yloxy)-1- (4-pyrro- 339 lidin-1-ylpiperidin-1-yl) ethanone ZON \ N 39 t 3 2-(4-Acetylphenoxy)-1-(4-pyrrolidin-1-331 ylpiperidin-1-yl) ethanone 0 Neo zon 40 CH3 3- (3-Methoxyphenyl)-1- (4-pyrrolidin- 317 1 1-ylpiperidin-1-yl) propan-1-one 0 j0 09 41 Nt 3-Pyridin-3-yl-1-(4-pyrrolidin-1-ylpi-288 0 NS-", peridin-1-yl) propan-1-one NzGN ZON G 42 3- (4-Benzyloxy-3-methoxyphenyl)-1- 421 (4-pyrrolidin-1-ylpiperidin-1-yl)- CH3 o propenone o O Zon 43 3-(5-Bromo-2-ethoxyphenyl)-1-(4-408 g \CH3 pyrrolidin-1-ylpiperidin-1-yl) propenone ber / 'U Br 44 1- (3, 4-Dihydro-2H-benzo [b] [1, 4]-387 dioxepin-7-yl)-4- (4-pyrrolidin-1- o o ylpiperidin-1-yl) butane-1, 4-dione Cj 45 3-(2-Chloro-3, 4-dimethoxyphenyl)-1-379 (4-pyrrolidin-1-ylpiperidin-1-yl)- cl o'CH3 propenone H3C 46 2-(2-Chloro-4-fluorophenylsulfanyl)-1-357 ( (4-pyrrolidin-1-ylpiperidin-1-yl)- N"O'ci F ethanone , N Cl F l 47 o 2-(Naphthalen-1-ylmethylsulfanyl)-1-369 (4-pyrrolidin-1-yipiperidin-1-yl)- ethanone W U 48 3- [3-Oxo-3- (4-pyrrolidin-1-yipiperidin- 344 1-yl) propyl]-3H-benzoxazol-2-one neo N' N 49 0 5-Cyclohexyl-1- (4-pyrrolidin-1- 321 ylpiperidin-1-yl) pentan-1-one ¢NAJ 50 H3c, 3- (4-Methoxyphenyl)-1- (4-pyrrolidin- 317 1-ylpiperidin-1-yl) propan-1-one zu zon GON 51 o 1-(4-Pyrrolidin-1-ylpiperidin-1-yl)-2-(4-373 o trifluoromethoxyphenoxy)-ethanone F F 52 2- (2-Acetylphenoxy)-1- (4-pyrrolidin-1- 331 O 9XCCH3 ylpiperidin-1-yl) ethanone 3 0 0 N 53 o Dimethylcarbamic acid 4- [3-oxo-3- (4- 374 0 pyrrolidin-1-yipiperidin-1-yl) propyl]- 0 CH3 phenyl ester 3 54 2- (5-Chloro-3-methyl-benzo [b] thio- 378 0 s ci phen-2-yl)-l- (4-pyrrolidin-I-yl- - piperidin-1-yl) ethanone G 55/% 3-Naphthalen-1-yl-1-(4-pyrrolidin-1-yl-335 piperidin-1-yl) propenone ON ---on "T I N4 56 otN+ o 3-(3-Nitro-4-pyrrolidin-1-ylphenyl)-1-399 (4-pyrrolidin-1-ylpiperidin-1-yl)- N propenone Y '-ON IF o 57 0 1- [1, 4'] Bipiperidinyl-1'-yl-3- (5-methyl- 303. 6 N 1H-imidazol-4-yl) propenone z N 58 0 1- [1, 4'] Bipiperidinyl-1'-yl-3- (5-methyl- 305. 4 N 1H-imidazol-4-yl) propan-1-one z N

The ability of the compounds to interact with the histamine H3 receptor can be de- termined by the following in vitro binding assays.

Binding assay (I) Rat cerebral cortex is homogenized in ice cold K-Hepes, 5 mM MgCI2 pH 7.1 buffer.

After two differential centrifugations the last pellet is resuspended in fresh Hepes buffer con- taining 1 mg/ml bacitracin. Aliquots of the membrane suspension (400 ug/ml) are incubated for 60 min at 25 °C with 30 pM [1251]-iodoproxifan, a known histamine H3 receptor antagonist, and the test compound at various concentrations. The incubation is stopped by dilution with ice-cold medium, followed by rapid filtration through Whatman GF/B filters pretreated for 1 hour with 0.5% polyethyleneimine. The radioactivity retained on the filters is counted using a Cobra II auto gamma counter. The radioactivity of the filters is indirectly proportional to the binding affinity of the tested compound. The results are analyzed by nonlinear regression analysis.

Binding assay (II) The H3-receptor agonist ligand R-a-methyl [3H] histamine (RAMHA) is incubated with isolated rat cortex cell-membranes at 25 °C for 1 hour, followed by a filtration of the incubate through Whatman GF/B filters. Radioactivity retained on the filters is measured using a beta counter.

Male Wistar rats (150-200 g) are decapitated and cerebral cortex is quickly dis- sected out and frozen immediately on dry ice. Tissue is kept at-80 °C until membrane pre- paration. During the membrane preparation the tissue is kept on ice all the time. Rat cerebral cortex is homogenized in 10 volumes (w/w) ice-cold Hepes buffer (20 mM Hepes, 5 mM

MgCl2 pH 7.1 (KOH) + 1 mg/ml bacitracin) using an Ultra-Turrax homogenizer for 30 sec- onds. The homogenate is centrifuged at 140 g in 10 min. The supernatant is transferred to a new test tube and centrifuged for 30 min at 23 000 g. Pellet is resuspended in 5-10 ml Hepes buffer, homogenized and centrifuged for 10 min at 23 000 g. This short centrifugation step is repeated twice. After the last centrifugation the pellet is resuspended in 2-4 mi Hepes buffer and the protein concentration is determined. The membranes are diluted to a protein concen- tration of 5 mg/ml using Hepes buffer, aliquote and stored at-80 °C until use.

50 pI test-compound, 100 pI membrane (200 lig/ml), 300 lli Hepes buffer and 50 R-a-methyl [3H] histamine (1 nM) are mixed in a test tube. The compounds to be tested are dissolved in DMSO and further diluted in Ha0 to the desired concentrations. Radioligand and membranes are diluted in Hepes buffer + 1 mg/ml bacitracin. The mixture is incubated for 60 min at 25 °C. Incubation is terminated by adding 5 ml ice-cold 0.9% NaCI, followed by rapid filtration through Whatman GF/B filters pre-treated for 1 hour with 0. 5% polyethyleneimine.

The filters are washed with 2 x 5 ml ice-cold NaCI. To each filter a 3 ml scintillation cocktail is added and the radioactivity retained is measured with a Packard Tri-Carb beta counter.

IC5o values are calculated by non-linear regression analysis of binding curves (6 points minimum) using the windows program GraphPad Prism, GraphPad software, USA.

Binding assay (III) The human H3 receptor is cloned by PCR and subcloned into the pcDNA3 expres- sion vector. Cells stably expressing the H3 receptor are generated by transfecting the H3- expression vectors into HEK 293 cells and using G418 to select for H3 clones. The human H3-HEK 293 clones are cultured in DMEM (GIBCO-BRL) with glutamax, 10% foetal calf se- rum, 1 % penicillin/streptavidin and 1 mg/ml G 418 at 37 °C and 5% COs. Before harvesting, the confluent cells are rinsed with PBS and incubated with Versene (proteinase, GIBCO- BRL) for approximately 5 min. The cells are flushed with PBS and DMEM and the cellsus- pension collected in a tube and centrifuged for 5-10 min at 1500 rpm in a Heraeus Sepatech Megafuge 1.0. The pellet is resuspended in 10-20 vol. Hepes buffer (20 mM Hepes, 5 mM MgCI2, pH 7.1 (KOH) ) and homogenized for 10-20 seconds using an Ultra-Turrax homoge- nizer. The homogenate is centrifuged for 30 min at 23 000 g. The pellet is resuspended in 5- 10 ml Hepes buffer, homogenized 5-10 seconds with the Ultra-Turrax and centrifuged for 10 min at 23 000 g. Following this centrifugation step, the membrane pellet is resuspended in 2- 4 mi Hepes buffer, homogenized with a syringe or teflon homogenizer, and the protein con- centration determined. The membranes are diluted to a protein concentration of 1-5 mg/ml in Hepes buffer, aliquote and kept at-80 °C until use.

Aliquots of the membrane suspension are incubated for 60 min at 25 °C with 30 pM [1251]-iodoproxifan, a known compound with high affinity for the H3 receptor, and the test compound at various concentrations. The incubation is stopped by dilution with ice-cold me- dium, followed by rapid filtration through Whatman GF/B filters pretreated for 1 hour with 0.5% polyethyleneimine. The radioactivity retained on the filters is counted using a Cobra II auto gamma counter. The radioactivity of the filters is indirectly proportional to the binding affinity of the tested compound. The results are analysed by nonlinear regression analysis.

When tested, some of the compounds show potent inverse agonism at the human histamine H3 receptor.

Preferably, the compounds according to the invention have an Iso value as deter- mined by one or more of the assays of less than 10 uM, more preferred of less than 1 suM, and even more preferred of less than 500 nM, such as of less than 100 nM.

Functional assay (I) The ability of the compounds to interact with the histamine H3 receptor as agonists, inverse agonists and/or antagonists, is determined by an in vitro functional assay utilizing membranes from HEK 293 cell expressing the human H3 receptors.

The H3 receptor is cloned by PCR and subcloned into the pcDNA3 expression vec- tor. Cells stably expressing the H3 receptor are generated by transfecting the H3-expression vectors into HEK 293 cells and using G418 to select for H3 clones. The human H3-HEK 293 clones are cultured in DMEM with glutamax, 10% foetal calf serum, 1% penicillin/streptavidin and 1 mg/ml G 418 at 37 °C and 5% CO2.

The H3 receptor expressing cells are washed once with phosphate buffered saline (PBS) and harvested using versene (GIBCO-BRL). PBS is added and the cells are centri- fuged for 5 min at 188 g. The cell pellet is resuspended in stimulation buffer to a concentra- tion of 1 x 106 cells/ml. cAMP accumulation is measured using the Flash Plate@ cAMP assay (NEN Life Science Products). The assay is generally performed as described by the manu- facturer. Briefly, 50 lui cela suspension is added to each well of the Flashplate which also con- tained 25 lli 40 Jv) isoprenaiine, to stimulate cAMP generation, and 25 gi of test compound (either agonists or inverse agonists alone, or agonist and antagonist in combination). The assay can be run in"agonist-mode"which means that the test compound is added, in in- creasing concentration, on its own, to the cells, and cAMP is measured. If cAMP goes up, it is an inverse agonist; if cAMP does not change, it is a neutral antagonist, and if cAMP goes down, it is an agonist. The assay can also be run in the"antagonist-mode"which means that a test compound is added, in increasing concentrations, together with increasing concentra-

tions of a known H3 agonist (eg RAMHA). If the compound is an antagonist, increasing con- centrations of it cause a right-ward shift in the H3-agonist's dose-response curves. The final volume in each well is 100 lli. Test compounds are dissolved in DMSO and diluted in H2O.

The mixture is shaken for 5 min, and allowed to stand for 25 min at room temperature. The reaction is stopped with 100 lli"Detection Mix"per well. The plates are then sealed with plas- tic, shaken for 30 min, allowed to stand overnight, and finally the radioactivity is counted in the Cobra II auto gamma topcounter. ECro values are calculated by non-linear regression analysis of dose response curves (6 points minimum) using GraphPad Prism. Kb values are calculated by Schild plot analysis.

The open cage Schedule-fed rat model The ability of the present compounds to reduce weight is determined using the in vivo open cage Schedule-fed rat model.

Sprague-Dawley (SD) male rats of an age of about 11/2 to 2 months and a weight of about 200-250 g are purchased from Mllegård Breeding and Research Centre A/S (Denmark). On arrival they are allowed some days of acclimatisation before being placed in individual open plastic cages. They are habituated to the presence of food (Altromin pelleted rat chow) in their home cage only during 7 hours in the morning from 07.30 to14. 30 h all days a week. Water is present ad libitum. As the consumption of food has stabilised after 7 to 9 days, the animals are ready for use.

Each animal is used only once to avoid carry-over effects between treatments.

During the test sessions, the test compound is administered intraperitoneally or orally 30 min before the start of the sessions. One group of animals is administered the test com- pound at different doses and a control group of animals is given a vehicle. Food and wa- ter intake are monitored at 1,2 and 3 hours post administration.

Any side effects may rapidly be discovered (barrel-rolling, bushy fur etc. ) since the animals are kept in transparent plastic cages to enable continuous monitoring.