Title:
SUBSTRATES FOR USE IN DETECTION OF PHOSPHORYLATION-DEPHOSPHORYLATION REACTION AND DETECTION METHOD USING THE SAME
Document Type and Number:
WIPO Patent Application WO/2006/083016
Kind Code:
A1
Abstract:
Disclosed is means for determining the reactivity of a protein kinase or a protein phosphatase in a simple manner. Substrates for a protein kinase, each comprising at least one artificial peptide substrate which is produced by substituting a serine residue in an amino acid sequence contained in a naturally occurring substrate for the protein kinase by a threonine residue; a peptide array comprising the substrates immobilized thereon; a method for detecting a phosphorylation reaction an/or a dephosphorylation reaction which is characterized by using the peptide array and an anti-phospho-threonine antibody; a method for diagnosing a disease based on the activation and/or inactivation of a protein kinase and/or a protein phosphatase which is characterized by using the peptide array and an anti-phospho-threonine antibody; and a method for screening a target substance which can vary the protein kinase or protein phosphatase activity which is characterized by using the peptide array and an anti-phospho-threonine antibody.
Inventors:
OKITSU OSAMU (JP)
NAGASHIMA TAKEYUKI (JP)
MURAMOTO MASAKAZU (JP)
WADA TAKUYA (JP)
NISHIMURA SHINTARO (JP)
KATAYAMA YOSHIKI (JP)
SONODA TATSUHIKO (JP)
KITA YASUHIRO (JP)
TAKAHASHI KIYOKO (JP)
TSUCHIDA AKIKO (JP)
NAGASHIMA TAKEYUKI (JP)
MURAMOTO MASAKAZU (JP)
WADA TAKUYA (JP)
NISHIMURA SHINTARO (JP)
KATAYAMA YOSHIKI (JP)
SONODA TATSUHIKO (JP)
KITA YASUHIRO (JP)
TAKAHASHI KIYOKO (JP)
TSUCHIDA AKIKO (JP)
Application Number:
PCT/JP2006/302216
Publication Date:
August 10, 2006
Filing Date:
February 02, 2006
Export Citation:
Assignee:
ASTELLAS PHARMA INC (JP)
OKITSU OSAMU (JP)
NAGASHIMA TAKEYUKI (JP)
MURAMOTO MASAKAZU (JP)
WADA TAKUYA (JP)
NISHIMURA SHINTARO (JP)
KATAYAMA YOSHIKI (JP)
SONODA TATSUHIKO (JP)
KITA YASUHIRO (JP)
TAKAHASHI KIYOKO (JP)
TSUCHIDA AKIKO (JP)
OKITSU OSAMU (JP)
NAGASHIMA TAKEYUKI (JP)
MURAMOTO MASAKAZU (JP)
WADA TAKUYA (JP)
NISHIMURA SHINTARO (JP)
KATAYAMA YOSHIKI (JP)
SONODA TATSUHIKO (JP)
KITA YASUHIRO (JP)
TAKAHASHI KIYOKO (JP)
TSUCHIDA AKIKO (JP)
International Classes:
C07K7/06; C07K7/08; C07K14/00; C07K17/02; C12Q1/48; G01N33/15; G01N33/50; G01N33/53
Foreign References:
| JP2004283114A | 2004-10-14 |
Other References:
COSMO BIO CO., LTD.: "Rinsanka signal", COSMO BIO NEWS 2003 TOKUSHUGO, 21 July 2003 (2003-07-21), pages 1 - 23, AND 34 - 39, AND TOKUBETSU FUROKU PAGES 1-16, XP003001578
LESAICHERRE M.L. ET AL.: "Antibody-based fluorescence detection of kinase activity on a peptide array", BIOORG. MED. CHEM. LETT., vol. 12, no. 16, 2002, pages 2085 - 2088, XP003001579
SONODA T. ET AL.: "Mass-tag technology for monitoring of protein kinase activity using mass spectrometry", BIOORG. MED. CHEM. LETT., vol. 14, no. 4, 23 February 2004 (2004-02-23), pages 847 - 850, XP003001580
LESAICHERRE M.L. ET AL.: "Antibody-based fluorescence detection of kinase activity on a peptide array", BIOORG. MED. CHEM. LETT., vol. 12, no. 16, 2002, pages 2085 - 2088, XP003001579
SONODA T. ET AL.: "Mass-tag technology for monitoring of protein kinase activity using mass spectrometry", BIOORG. MED. CHEM. LETT., vol. 14, no. 4, 23 February 2004 (2004-02-23), pages 847 - 850, XP003001580
Attorney, Agent or Firm:
Takashima, Hajime (Osaka Midosuji Bldg. 1-1, Fushimimachi
4-chome, Chuo-k, Osaka-shi Osaka 44, JP)
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