Title:
SUGAR KINASES WITH EXPANDED SUBSTRATE SPECIFICITY AND THEIR USE
Document Type and Number:
WIPO Patent Application WO2005056786
Kind Code:
A3
Abstract:
One preferred embodiment of the present invention provides a GalK variant comprising a R28, E34, D37, D174, Y233, C339, Y371, Y371H, M173, M173L, Y371H-M173L and C353 mutation. The GalK variants display catalytic activity toward a variety of D or L sugars. Another preferred embodiment provides method of phosphorylating sugars comprising the step of incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant such that a sugar phosphate is produced. This sugar phosphate may be further incubated with a nucleotidylyltransferase, such that a NDP-sugar is produced. The NDP-sugar may be further incubated with a biomolecule to produce a glycosylated biomolecule.
Inventors:
THORSON JON S (US)
Application Number:
PCT/US2004/040942
Publication Date:
September 01, 2005
Filing Date:
December 06, 2004
Export Citation:
Assignee:
WISCONSIN ALUMNI RES FOUND (US)
THORSON JON S (US)
THORSON JON S (US)
International Classes:
C07H5/06; C07H21/04; C08B37/00; C12N1/21; C12N9/12; C12N15/74; C12P19/18; (IPC1-7): C12N9/12; C12N15/12; C12P19/18
Domestic Patent References:
| WO2002079150A2 | 2002-10-10 | |||
| WO2002048331A2 | 2002-06-20 |
Other References:
HOFFMEISTER DIRK ET AL: "Creation of the first anomeric D/L-sugar kinase by means of directed evolution.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 11 NOV 2003, vol. 100, no. 23, 11 November 2003 (2003-11-11), pages 13184 - 13189, XP002332872, ISSN: 0027-8424
YANG JIE ET AL: "Studies on the substrate specificity of Escherichia coli galactokinase.", ORGANIC LETTERS. 26 JUN 2003, vol. 5, no. 13, 26 June 2003 (2003-06-26), pages 2223 - 2226, XP002332873, ISSN: 1523-7060
YANG JIE ET AL: "Natural product glycorandomization.", BIOORGANIC & MEDICINAL CHEMISTRY. 1 APR 2004, vol. 12, no. 7, 1 April 2004 (2004-04-01), pages 1577 - 1584, XP002320075, ISSN: 0968-0896
YANG JIE ET AL: "Studies on the substrate specificity of Escherichia coli galactokinase.", ORGANIC LETTERS. 26 JUN 2003, vol. 5, no. 13, 26 June 2003 (2003-06-26), pages 2223 - 2226, XP002332873, ISSN: 1523-7060
YANG JIE ET AL: "Natural product glycorandomization.", BIOORGANIC & MEDICINAL CHEMISTRY. 1 APR 2004, vol. 12, no. 7, 1 April 2004 (2004-04-01), pages 1577 - 1584, XP002320075, ISSN: 0968-0896
Download PDF: