Title:
SULFATED ABALONE POLYSACCHARIDE AND APPLICATION THEREOF IN INHIBITING SARS-COV-2
Document Type and Number:
WIPO Patent Application WO/2022/217763
Kind Code:
A1
Abstract:
Provided is a preparation method for a sulfated abalone polysaccharide, which comprises the following steps: S1, stirring anhydrous pyridine in an ice bath until evenly mixed, and gradually adding chlorosulfuric acid in a dropwise fashion to obtain an esterification agent; S2, dissolving abalone crude polysaccharide freeze-dried powder in N, N-dimethylformamide, adding the esterification agent, stirring at a constant temperature of 30-80 °C and reacting for 1-8 hours, cooling to room temperature, and adding pre-cooled water; and S3, adjusting to a neutral pH value, stirring while adding anhydrous ethanol, and after allowing to sit, and centrifuging to obtain a precipitate, and re-dissolving in water, then performing dialysis after filtering, and upon freeze-drying, obtaining a sulfated abalone polysaccharide. The sulfated abalone polysaccharide of the present invention can effectively inhibit a transfection effect of the SARS-CoV-2 virus on cells, and an inhibiting effect on SARS-CoV-2 is 3.3 times that of a regular abalone polysaccharide.
Inventors:
YANG JINGFENG (CN)
SUN JINGHE (CN)
YAN TINGTING (CN)
SONG SHUANG (CN)
ZHU BEIWEI (CN)
SUN JINGHE (CN)
YAN TINGTING (CN)
SONG SHUANG (CN)
ZHU BEIWEI (CN)
Application Number:
PCT/CN2021/104455
Publication Date:
October 20, 2022
Filing Date:
July 05, 2021
Export Citation:
Assignee:
UNIV DALIAN POLYTECHNIC (CN)
International Classes:
C08B37/00; A01N43/16; A01P1/00; A01P3/00; A61K31/737; A61K35/618; A61P31/14
Foreign References:
| CN111588732A | 2020-08-28 | |||
| CN111658664A | 2020-09-15 | |||
| CN111773240A | 2020-10-16 | |||
| CN104558228A | 2015-04-29 | |||
| CN111454376A | 2020-07-28 | |||
| CN110041442A | 2019-07-23 | |||
| CN103739733A | 2014-04-23 | |||
| CN111560080A | 2020-08-21 | |||
| CN112220799A | 2021-01-15 | |||
| CN111803515A | 2020-10-23 | |||
| JP2008266265A | 2008-11-06 |
Other References:
YIM SUNG-KUN, KIM KIAN, KIM INHEE, CHUN SANGHO, OH TAEHWAN, KIM JIN-UNG, KIM JUNGWON, JUNG WOOHUK, MOON HOSANG, KU BOSUNG, JUNG KY: "Inhibition of SARS-CoV-2 Virus Entry by the Crude Polysaccharides of Seaweeds and Abalone Viscera In Vitro", MARINE DRUGS, vol. 19, no. 4, pages 219, XP055844460, DOI: 10.3390/md19040219
LIU, YILI; DUAN MENGMENG; SUN XIAONA; JIANG PINGRUI; SONG SHUANG: "Effect of Sulfate Group in Polysaccharide of Pacific Abalone on High Fat Diet-Induced Metabolic Syndrome in Mice", ABSTRACTS OF FOOD SUMMIT IN CHINA 2019 & 16TH ANNUAL MEETING OF CIFST; WUHAN, CHINA; NOVEMBER 13, 2019, 30 November 2019 (2019-11-30), pages 155 - 156, XP009541869
WANG ZHIJUN, XIE JIANHUA, SHEN MINGYUE, NIE SHAOPING, XIE MINGYONG: "Sulfated modification of polysaccharides: Synthesis, characterization and bioactivities", TRENDS IN FOOD SCIENCE & TECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, GB, vol. 74, 1 April 2018 (2018-04-01), GB , pages 147 - 157, XP055976973, ISSN: 0924-2244, DOI: 10.1016/j.tifs.2018.02.010
SHUANG SONG, HAORAN PENG, QINGLING WANG, ZHENGQI LIU, XIUPING DONG, CHENGRONG WEN, CHUNQING AI, YUJIAO ZHANG, ZHONGFU WANG, BEIWEI: "Inhibitory activities of marine sulfated polysaccharides against SARS-CoV-2", FOOD & FUNCTION, R S C PUBLICATIONS, GB, vol. 11, no. 9, 23 September 2020 (2020-09-23), GB , pages 7415 - 7420, XP055758218, ISSN: 2042-6496, DOI: 10.1039/D0FO02017F
PAUL S. KWON, HANSEUL OH, SEOK-JOON KWON, WEIHUA JIN, FUMING ZHANG, KEITH FRASER, JUNG JOO HONG, ROBERT J. LINHARDT, JONATHAN S. D: "Sulfated polysaccharides effectively inhibit SARS-CoV-2 in vitro", CELL DISCOVERY, vol. 6, no. 1, 1 December 2020 (2020-12-01), XP055758214, DOI: 10.1038/s41421-020-00192-8
LIU, YILI; DUAN MENGMENG; SUN XIAONA; JIANG PINGRUI; SONG SHUANG: "Effect of Sulfate Group in Polysaccharide of Pacific Abalone on High Fat Diet-Induced Metabolic Syndrome in Mice", ABSTRACTS OF FOOD SUMMIT IN CHINA 2019 & 16TH ANNUAL MEETING OF CIFST; WUHAN, CHINA; NOVEMBER 13, 2019, 30 November 2019 (2019-11-30), pages 155 - 156, XP009541869
WANG ZHIJUN, XIE JIANHUA, SHEN MINGYUE, NIE SHAOPING, XIE MINGYONG: "Sulfated modification of polysaccharides: Synthesis, characterization and bioactivities", TRENDS IN FOOD SCIENCE & TECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, GB, vol. 74, 1 April 2018 (2018-04-01), GB , pages 147 - 157, XP055976973, ISSN: 0924-2244, DOI: 10.1016/j.tifs.2018.02.010
SHUANG SONG, HAORAN PENG, QINGLING WANG, ZHENGQI LIU, XIUPING DONG, CHENGRONG WEN, CHUNQING AI, YUJIAO ZHANG, ZHONGFU WANG, BEIWEI: "Inhibitory activities of marine sulfated polysaccharides against SARS-CoV-2", FOOD & FUNCTION, R S C PUBLICATIONS, GB, vol. 11, no. 9, 23 September 2020 (2020-09-23), GB , pages 7415 - 7420, XP055758218, ISSN: 2042-6496, DOI: 10.1039/D0FO02017F
PAUL S. KWON, HANSEUL OH, SEOK-JOON KWON, WEIHUA JIN, FUMING ZHANG, KEITH FRASER, JUNG JOO HONG, ROBERT J. LINHARDT, JONATHAN S. D: "Sulfated polysaccharides effectively inhibit SARS-CoV-2 in vitro", CELL DISCOVERY, vol. 6, no. 1, 1 December 2020 (2020-12-01), XP055758214, DOI: 10.1038/s41421-020-00192-8
Attorney, Agent or Firm:
DALIAN GEZHI INTELLECTUAL PROPERTY AGENT LTD. (CN)
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