Title:
SUPER-RESOLUTION MICROSCOPIC IMAGING METHOD AND APPARATUS BASED ON SHARED-PATH PARALLEL FLUORESCENCE EMISSION DIFFERENCE
Document Type and Number:
WIPO Patent Application WO/2022/111602
Kind Code:
A1
Abstract:
A super-resolution microscopic imaging method and apparatus based on a shared-path parallel fluorescence emission difference. Exciting light during fluorescence emission difference microscopy imaging is modulated by using a liquid crystal spatial light modulator (6). Two parts of the liquid crystal spatial light modulator (6) are respectively loaded as 0-2 π vortex phase modulation and a blazed grating, such that a solid light spot and a hollow light spot, which are at a certain distance from each other, are simultaneously formed on a sample surface by exciting light that shares a path, and are subjected to parallel scanning, thereby doubling the imaging speed compared with common fluorescence emission difference microscopy imaging, and also making two beams of exciting light not be susceptible to noise and drift interference due to sharing of the path.
Inventors:
KUANG CUIFANG (CN)
HUANG YURAN (CN)
ZHANG ZHIMIN (CN)
LIU SHAOCONG (CN)
LIU XU (CN)
HUANG YURAN (CN)
ZHANG ZHIMIN (CN)
LIU SHAOCONG (CN)
LIU XU (CN)
Application Number:
PCT/CN2021/133299
Publication Date:
June 02, 2022
Filing Date:
November 25, 2021
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
International Classes:
G01N21/64; G01J3/44; G02B21/00
Foreign References:
| CN112649405A | 2021-04-13 | |||
| CN109632756A | 2019-04-16 | |||
| US20090250632A1 | 2009-10-08 | |||
| CN103411941A | 2013-11-27 | |||
| CN110632045A | 2019-12-31 | |||
| CN110118726A | 2019-08-13 | |||
| CN111781173A | 2020-10-16 | |||
| CN108120702A | 2018-06-05 |
Attorney, Agent or Firm:
HANGZHOU QIUSHI PATENT OFFICE CO., LTD. (CN)
Download PDF:
Previous Patent: EYE MOVEMENT TRACKING APPARATUS AND ELECTRONIC DEVICE
Next Patent: SAMPLE ANALYSIS DEVICE AND ANALYSIS METHOD
Next Patent: SAMPLE ANALYSIS DEVICE AND ANALYSIS METHOD