Title:
SUPPRESSION OF TARGETED APTAMER CLUSTER
Document Type and Number:
WIPO Patent Application WO/2024/009947
Kind Code:
A1
Abstract:
The present disclosure, at least in part, relates to methods of sequencing low abundance aptamers from an aptamer library, the method comprising: (i) amplifying a plurality of aptamers capable of binding to one or more target molecules in a sample by emulsion PCR to generate an aptamer library; (ii) sequencing the aptamer library; and (iii) contacting a plurality of antisense oligonucleotides (ASOs) targeting high abundance aptamers of the aptamer library with the aptamer library to form a mixture, wherein contacting the ASOs with the aptamer library results in inactivation of the high abundance aptamers of the aptamer library.
Inventors:
SAKAKIBARA YOGO (JP)
Application Number:
PCT/JP2023/024609
Publication Date:
January 11, 2024
Filing Date:
July 03, 2023
Export Citation:
Assignee:
EISAI R&D MAN CO LTD (JP)
International Classes:
C12N15/115; C12Q1/686; C12Q1/6876; C40B30/10
Foreign References:
| US20090264508A1 | 2009-10-22 |
Other References:
CHAI CHANGHOON: "Principle of Emulsion PCR and Its Applications in Biotechnology", JOURNAL OF ANIMAL REPRODUCTION AND BIOTECHNOLOGY, KOREAN SOCIETY OF ANIMAL REPRODUCTION AND BIOTECHNOLOGY, vol. 34, no. 4, 31 December 2019 (2019-12-31), pages 259 - 266, XP055878906, ISSN: 2671-4639, DOI: 10.12750/JARB.34.4.259
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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