Title:
SYSTEM FOR EFFICIENTLY CATALYZING ALIPHATIC DINITRILE HYDRATION REACTION USING NEW NITRILE HYDRATASE
Document Type and Number:
WIPO Patent Application WO/2020/155690
Kind Code:
A1
Abstract:
Provided is a system for efficiently catalyzing an aliphatic dinitrile hydration reaction using a new nitrile hydratase. Provided is the brand-new use of nitrile hydratase from Rhodococcus erythropolis CCM2595 in the catalysis of aliphatic dinitrile. Particularly, the nitrile hydratase can selectively catalyze adiponitrile to generate 5-cyanovaleramide, with a high reaction rate and simple and mild reaction conditions, and same provides a method for the industrial production of 5-cyanovaleramide.
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Inventors:
LIANG CHANGHAI (CN)
WANG LI (CN)
LIU SHENGXIAN (CN)
DOU TONGYI (CN)
CUI CHANGHAO (CN)
WANG LI (CN)
LIU SHENGXIAN (CN)
DOU TONGYI (CN)
CUI CHANGHAO (CN)
Application Number:
PCT/CN2019/113301
Publication Date:
August 06, 2020
Filing Date:
October 25, 2019
Export Citation:
Assignee:
UNIV DALIAN TECH (CN)
International Classes:
C12N15/70; C12N15/60; C12P13/02
Foreign References:
| CN101619299A | 2010-01-06 | |||
| CN109797164A | 2019-05-24 |
Other References:
RUCKA, L.: "Expression control of nitrile hydratase and amidase genes in Rhodococcus erythropolis and substrate specificities of the enzymes", ANTONIE VAN LEEUWENHOEK, vol. 105, no. 6, June 2014 (2014-06-01), pages 1179 - 1190, XP055726219
KUBAC, D.: "Biotransformation of nitriles to amides using soluble and immobilized nitrile hydratase from Rhodococcus erythropolis A4", JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC, vol. 50, 11 September 2007 (2007-09-11), pages 107 - 113, XP022387858
KAMBLE, A. L.: "Nitrile hydratase of Rhodococcus erythropolis:characterization of the enzyme and the use of whole cells for biotransformation of nitriles", 3 BIOTECH., vol. 3, no. 4, 16 December 2012 (2012-12-16), pages 319 - 330, XP055726220
MOREAU, J. L.: "Application of High-performance liquid chromatography to the study of the biological transformation of adiponitrile by nitrile hydra- tase and amidase", ANALYST., vol. 116, no. 12, 31 December 1991 (1991-12-31), pages 1381 - 1383, XP055726221
STRNAD, H.: "Genome sequence of Rhodococcus erythropolis strain CCM2595, a phenol derivative-degrading bacterium", GENOME ANNOUNCEMENTS, vol. 2, no. 2, 27 March 2014 (2014-03-27), pages e00208-14-1 - e00208-14-2, XP002731744
ALENA ČEJKOVÁ ET AL: "Potential of Rhodococcus erythropolis as a bioremediation organism", WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, vol. 21, no. 3, 1 April 2005 (2005-04-01), pages 317 - 321, XP019271522
KUBAC, D.: "Biotransformation of nitriles to amides using soluble and immobilized nitrile hydratase from Rhodococcus erythropolis A4", JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC, vol. 50, 11 September 2007 (2007-09-11), pages 107 - 113, XP022387858
KAMBLE, A. L.: "Nitrile hydratase of Rhodococcus erythropolis:characterization of the enzyme and the use of whole cells for biotransformation of nitriles", 3 BIOTECH., vol. 3, no. 4, 16 December 2012 (2012-12-16), pages 319 - 330, XP055726220
MOREAU, J. L.: "Application of High-performance liquid chromatography to the study of the biological transformation of adiponitrile by nitrile hydra- tase and amidase", ANALYST., vol. 116, no. 12, 31 December 1991 (1991-12-31), pages 1381 - 1383, XP055726221
STRNAD, H.: "Genome sequence of Rhodococcus erythropolis strain CCM2595, a phenol derivative-degrading bacterium", GENOME ANNOUNCEMENTS, vol. 2, no. 2, 27 March 2014 (2014-03-27), pages e00208-14-1 - e00208-14-2, XP002731744
ALENA ČEJKOVÁ ET AL: "Potential of Rhodococcus erythropolis as a bioremediation organism", WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, vol. 21, no. 3, 1 April 2005 (2005-04-01), pages 317 - 321, XP019271522
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