Title:
SYSTEMS AND METHODS FOR SCREENING OF LARGE GENE LIBRARIES
Document Type and Number:
WIPO Patent Application WO/2024/073375
Kind Code:
A3
Abstract:
Library screening is an important analytic tool for identifying functional nucleic acids or proteins. In some aspects, droplet-based systems and methods for library screening are provided. The systems and methods may include steps of sorting droplets based on activity, amplifying nucleic acids of "activity droplets" having high activity, and separating nucleic acids from the activity droplets into new droplets. The steps may be iterated, such that activity droplets from successive iterations tend to include fewer nucleic acids. Such approaches may facilitate identification of active nucleic acids, or of nucleic acids encoding active proteins.
Inventors:
WEITZ DAVID (US)
MILCIC KARLA (US)
ZHANG XINGE (US)
CHEN ANQI (US)
MILCIC KARLA (US)
ZHANG XINGE (US)
CHEN ANQI (US)
Application Number:
PCT/US2023/075065
Publication Date:
May 02, 2024
Filing Date:
September 25, 2023
Export Citation:
Assignee:
HARVARD COLLEGE (US)
International Classes:
C12P19/34; C12N15/10; C12Q1/6806; C12Q1/6869
Attorney, Agent or Firm:
CHEN, Tani et al. (US)
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