Title:
TARGET NUCLEIC ACID AMPLIFICATION METHOD USING GUIDE PROBE AND CLAMPING PROBE AND COMPOSITION FOR AMPLIFYING TARGET NUCLEIC ACID COMPRISING SAME
Document Type and Number:
WIPO Patent Application WO/2022/203297
Kind Code:
A1
Abstract:
The present invention relates to: a target nucleic acid amplification method capable of amplifying a very low concentration of a target nucleic acid with high specificity; and a composition for amplifying a target nucleic acid by using same and, more specifically, to: a method for producing an amplicon of a target nucleic acid with high specificity by using, when a target nucleic acid is present, a guide probe binding to the target nucleic acid, a partial primer capable of amplifying the target nucleic acid by binding to the guide probe, and a clamping probe that inhibits the amplification of nucleic acids other than the target nucleic acid; and a composition for a polymerase chain reaction (PCR) therefor. The target nucleic acid amplification method according to the present invention is advantageous in that a target nucleic acid present at a very low concentration can be amplified using a guide probe which binds to all hybridizable nucleic acids present in a sample and helps a partial primer bind to a target nucleic acid detection site, nucleic acids other than the target nucleic acid differ in amplification rate due to the sequence specificity of the partial primer, and the target nucleic acid can be detected with high specificity since the amplification of nucleic acids other than the target nucleic acid is suppressed by a clamping probe, and thus is effective in molecular diagnosis, prenatal diagnosis, early diagnosis, cancer diagnosis, heredity-related diagnosis, genetic trait diagnosis, diagnosis of infectious bacteria, identification of drug-resistant bacteria, forensic medicine, classification of the species of organisms, and the like.
Inventors:
PARK JAEJIN (KR)
KIM MINSEO (KR)
NAM HYO JOO (KR)
LEE YOUNG (KR)
KIM MINSEO (KR)
NAM HYO JOO (KR)
LEE YOUNG (KR)
Application Number:
PCT/KR2022/003869
Publication Date:
September 29, 2022
Filing Date:
March 21, 2022
Export Citation:
Assignee:
PANAGENE INC (KR)
International Classes:
C12Q1/686
Domestic Patent References:
| WO2019178346A1 | 2019-09-19 |
Foreign References:
| KR20200068738A | 2020-06-15 | |||
| US20080176226A1 | 2008-07-24 | |||
| KR101830700B1 | 2018-02-21 | |||
| KR20200066712A | 2020-06-10 | |||
| KR102019800B1 | 2019-09-10 |
Other References:
KIM MEYONG IL, LEE KI-YOUNG, CHO SANG-MAN: "Development of Clamping Probe for Rare DNA Detection using Universal Primers", FISHERIES AND AQUATIC SCIENCES, vol. 17, no. 3, 30 September 2014 (2014-09-30), pages 339 - 344, XP055971925, ISSN: 2234-1749, DOI: 10.5657/FAS.2014.0339
HIGUCHI, R. ET AL., BIOTECHNOLOGY, vol. 10, 1992, pages 413 - 417
HIGUCHI, R. ET AL., BIOTECHNOLOGY, vol. 11, 1993, pages 1026 - 1030
SUN, X. ET AL., NAT. BIOTECHNOL., vol. 20, 2002, pages 186 - 189
DOMINGUEZ, P. L. ET AL., ONCOGENE, vol. 24, 2005, pages 6830 - 6834
LI, J. ET AL., NAT. MED, vol. 14, 2008, pages 579 - 584
NEWTON, C. R. ET AL., NUCL. ACIDS RES., vol. 17, 1989, pages 2503 - 2516
DRENKARD, E. ET AL., PLANT PHYSIOL., vol. 124, 2000, pages 1483 - 1492
WATERS, A. M.DER, C. J., COLD SPRING HARB. PERSPECT. MED., vol. 8, 2018
HIGUCHI, R. ET AL., BIOTECHNOLOGY, vol. 10, 1992, pages 413 - 417
HIGUCHI, R. ET AL., BIOTECHNOLOGY, vol. 11, 1993, pages 1026 - 1030
SUN, X. ET AL., NAT. BIOTECHNOL., vol. 20, 2002, pages 186 - 189
DOMINGUEZ, P. L. ET AL., ONCOGENE, vol. 24, 2005, pages 6830 - 6834
LI, J. ET AL., NAT. MED, vol. 14, 2008, pages 579 - 584
NEWTON, C. R. ET AL., NUCL. ACIDS RES., vol. 17, 1989, pages 2503 - 2516
DRENKARD, E. ET AL., PLANT PHYSIOL., vol. 124, 2000, pages 1483 - 1492
WATERS, A. M.DER, C. J., COLD SPRING HARB. PERSPECT. MED., vol. 8, 2018
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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