Title:
TARGET NUCLEIC ACID DETECTION METHOD BASED ON PROXIMITY PROTEOLYSIS REACTION
Document Type and Number:
WIPO Patent Application WO/2020/071777
Kind Code:
A1
Abstract:
The present invention relates to a method for detecting a target nucleic acid, comprising: (a) a step of mixing a sample containing the target nucleic acid with a nucleic acid detection solution comprising i) ssDNA-protease conjugate, ii) ssDNA-zymogen conjugate, and iii) a substrate specific for the zymogen; and (b) a step of detecting a signal generated by a proximity proteolysis reaction of the ssDNA-zymogen conjugate and the ssDNA-protease conjugate hybridized to the target nucleic acid.
Inventors:
YOO TAE HYEON (KR)
PARK HYEON JI (KR)
PARK HYEON JI (KR)
Application Number:
PCT/KR2019/012891
Publication Date:
April 09, 2020
Filing Date:
October 02, 2019
Export Citation:
Assignee:
UNIV AJOU IND ACADEMIC COOP FOUND (KR)
International Classes:
C12Q1/6818
Foreign References:
| US20170315114A1 | 2017-11-02 | |||
| US20030198971A1 | 2003-10-23 | |||
| KR101695684B1 | 2017-01-13 |
Other References:
KIM, HAJIN ET AL.: "An engineering of p-lactamase zymogen to establish a protease assay method", KSBB CONFERENCE, April 2017 (2017-04-01), pages 233
KIM, HAJIN ET AL.: "Development, of a colorimetric protease assay method using an engineered beta-laclamase zymogen.", KSBB CONFERENCE, April 2016 (2016-04-01), pages 300
YAN, LEI ET AL.: "Isothermal amplified detection of DNA and RNA", MOLECULAR BIOSYSTEMS, vol. 10, 2014, pages 970 - 1003, XP055324229
PARK, HYEON JI ET AL.: "Nucleic acid detection by a target-assisted proximity proteolysis reaction", ACS SENSORS, vol. 3, 8 October 2018 (2018-10-08), pages 2066 - 2070, XP055704545
KIM, HAJIN ET AL.: "Development, of a colorimetric protease assay method using an engineered beta-laclamase zymogen.", KSBB CONFERENCE, April 2016 (2016-04-01), pages 300
YAN, LEI ET AL.: "Isothermal amplified detection of DNA and RNA", MOLECULAR BIOSYSTEMS, vol. 10, 2014, pages 970 - 1003, XP055324229
PARK, HYEON JI ET AL.: "Nucleic acid detection by a target-assisted proximity proteolysis reaction", ACS SENSORS, vol. 3, 8 October 2018 (2018-10-08), pages 2066 - 2070, XP055704545
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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