Title:
TARGET SYSTEM FOR HOMOLOGY-DIRECTED REPAIR AND GENE EDITING METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2023/153845
Kind Code:
A3
Abstract:
The present invention relates to a novel target nucleic acid editing system including a miniaturized nucleic acid editing protein and an engineered guide RNA, uses of homology-directed repair (HDR) in a target gene thereof, and the like. According to one embodiment, homology-directed repair (HDR) using the target nucleic acid editing system has high HDR efficiency compared to other CRISPR/Cas systems due to a tendency to cut the back (outside) of a target nucleic acid, and also has the effect of enabling packaging of a gene editing system including a donor nucleic acid in a single vector, even when the packaging size is very limited like adeno-associated viruses (AAV), and maximizing the HDR efficiency by adding shRNA that inhibits a non-homologous end joining (NHEJ) process.
Inventors:
KIM YONG SAM (KR)
KIM DO YON (KR)
LEE JEONG MI (KR)
CHIN HYUN JUNG (KR)
KIM DO YON (KR)
LEE JEONG MI (KR)
CHIN HYUN JUNG (KR)
Application Number:
PCT/KR2023/001945
Publication Date:
April 11, 2024
Filing Date:
February 09, 2023
Export Citation:
Assignee:
GENKORE CO LTD (KR)
International Classes:
C12N15/63; C12N9/22; C12N15/113
Attorney, Agent or Firm:
SEUM IP (KR)
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