Title:
TAT EXPRESSION SYSTEM
Document Type and Number:
WIPO Patent Application WO/2019/038555
Kind Code:
A3
Abstract:
The present invention relates to an expression system for the production of recombinant proteins, in particular a twin arginine translocase (Tat) expression system. The present invention describes a cell that is modified with the Tat expression system, cultures comprising the cell, and a method of producing recombinant polypeptides using the cell. Improved signal peptides for recombinant protein secretion via the Tat pathway are also described.
Inventors:
BROWNING DOUGLAS (GB)
ROBINSON COLIN (GB)
ROBINSON COLIN (GB)
Application Number:
PCT/GB2018/052403
Publication Date:
April 04, 2019
Filing Date:
August 23, 2018
Export Citation:
Assignee:
UNIV BIRMINGHAM (GB)
THE UNIV OF KENT (GB)
THE UNIV OF KENT (GB)
International Classes:
C12P21/00; C12N15/63
Domestic Patent References:
| WO2002022667A2 | 2002-03-21 | |||
| WO2003040335A2 | 2003-05-15 |
Foreign References:
| US20140154742A1 | 2014-06-05 |
Other References:
DOUGLAS F. BROWNING ET AL: "Escherichia coli "TatExpress" strains super-secrete human growth hormone into the bacterial periplasm by the Tat pathway", BIOTECHNOLOGY AND BIOENGINEERING, vol. 114, no. 12, 6 October 2017 (2017-10-06), US, pages 2828 - 2836, XP055527816, ISSN: 0006-3592, DOI: 10.1002/bit.26434
CRISTINA F.R.O MATOS ET AL: "High-yield export of a native heterologous protein to the periplasm by the tat translocation pathway in Escherichia coli", BIOTECHNOLOGY AND BIOENGINEERING, 1 October 2012 (2012-10-01), Hoboken, pages 2533 - 2542, XP055527822, Retrieved from the Internet DOI: 10.1002/bit.24535
ANTHONY J. SNYDER ET AL: "ABSTRACT", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 80, no. 10, 15 May 2014 (2014-05-15), US, pages 3219 - 3232, XP055527828, ISSN: 0099-2240, DOI: 10.1128/AEM.00335-14
STEVEN D. BRANSTON ET AL: "Investigation of the impact of Tat export pathway enhancement on E. coli culture, protein production and early stage recovery", BIOTECHNOLOGY AND BIOENGINEERING, vol. 109, no. 4, 1 April 2012 (2012-04-01), US, pages 983 - 991, XP055528344, ISSN: 0006-3592, DOI: 10.1002/bit.24384
XIONG ET AL: "Expression level of heterologous tat genes is crucial for in vivo reconstitution of a functional Tat translocase in Escherichia coli", BIOCHIMIE, MASSON, PARIS, FR, vol. 89, no. 5, 3 May 2007 (2007-05-03), pages 676 - 685, XP022081073, ISSN: 0300-9084, DOI: 10.1016/J.BIOCHI.2007.01.006
RIBNICKY ET AL: "A scFv Antibody Mutant Isolated in a Genetic Screen for Improved Export via the Twin Arginine Transporter Pathway Exhibits Faster Folding", JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS, UNITED KINGDOM, vol. 369, no. 3, 11 May 2007 (2007-05-11), pages 631 - 639, XP022077558, ISSN: 0022-2836, DOI: 10.1016/J.JMB.2007.03.068
BEN C. BERKS: "The Twin-Arginine Protein Translocation Pathway", ANNUAL REVIEW OF BIOCHEMISTRY, vol. 84, no. 1, 2 June 2015 (2015-06-02), US, pages 843 - 864, XP055527808, ISSN: 0066-4154, DOI: 10.1146/annurev-biochem-060614-034251
CRISTINA F.R.O MATOS ET AL: "High-yield export of a native heterologous protein to the periplasm by the tat translocation pathway in Escherichia coli", BIOTECHNOLOGY AND BIOENGINEERING, 1 October 2012 (2012-10-01), Hoboken, pages 2533 - 2542, XP055527822, Retrieved from the Internet
ANTHONY J. SNYDER ET AL: "ABSTRACT", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 80, no. 10, 15 May 2014 (2014-05-15), US, pages 3219 - 3232, XP055527828, ISSN: 0099-2240, DOI: 10.1128/AEM.00335-14
STEVEN D. BRANSTON ET AL: "Investigation of the impact of Tat export pathway enhancement on E. coli culture, protein production and early stage recovery", BIOTECHNOLOGY AND BIOENGINEERING, vol. 109, no. 4, 1 April 2012 (2012-04-01), US, pages 983 - 991, XP055528344, ISSN: 0006-3592, DOI: 10.1002/bit.24384
XIONG ET AL: "Expression level of heterologous tat genes is crucial for in vivo reconstitution of a functional Tat translocase in Escherichia coli", BIOCHIMIE, MASSON, PARIS, FR, vol. 89, no. 5, 3 May 2007 (2007-05-03), pages 676 - 685, XP022081073, ISSN: 0300-9084, DOI: 10.1016/J.BIOCHI.2007.01.006
RIBNICKY ET AL: "A scFv Antibody Mutant Isolated in a Genetic Screen for Improved Export via the Twin Arginine Transporter Pathway Exhibits Faster Folding", JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS, UNITED KINGDOM, vol. 369, no. 3, 11 May 2007 (2007-05-11), pages 631 - 639, XP022077558, ISSN: 0022-2836, DOI: 10.1016/J.JMB.2007.03.068
BEN C. BERKS: "The Twin-Arginine Protein Translocation Pathway", ANNUAL REVIEW OF BIOCHEMISTRY, vol. 84, no. 1, 2 June 2015 (2015-06-02), US, pages 843 - 864, XP055527808, ISSN: 0066-4154, DOI: 10.1146/annurev-biochem-060614-034251
Attorney, Agent or Firm:
HGF LIMITED (GB)
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