Title:
TECHNOLOGY FOR MODULAR ASSEMBLY OF CELL-PENETRATING PEPTIDE-MEDIATED POLYPEPTIDE OR MICROPROTEIN TARGETING CHIMERA, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/274347
Kind Code:
A1
Abstract:
Provided are a technology for modular assembly of a cell-penetrating peptide-mediated polypeptide or microprotein targeting chimera, and the use thereof. The targeting chimera comprises at least one cell-penetrating peptide module, at least one targeting polypeptide module and at least one small molecule ligand module which are mutually connected, wherein the targeting polypeptide module is a polypeptide sequence capable of binding to a targeting target protein. The technology has the following characteristics and advantages of the provided cell-penetrating peptide-mediated polypeptide or microprotein modularly-assembled targeting chimera being designed in a modular manner, virous sequences or small molecule compound modules having different functions being able to be replaced and overlapped according to requirements, and all the polypeptide module parts being able to be subjected to cyclization or secondary microprotein structure modification. The design idea greatly enhances the use effect and application range of a targeting drug.
Inventors:
LIU MIAO (CN)
Application Number:
PCT/CN2022/102648
Publication Date:
January 05, 2023
Filing Date:
June 30, 2022
Export Citation:
Assignee:
LIU MIAO (CN)
International Classes:
C07K7/06; A61K47/42
Foreign References:
| CN108948178A | 2018-12-07 | |||
| CN107794280A | 2018-03-13 | |||
| CN111225976A | 2020-06-02 | |||
| US20160264627A1 | 2016-09-15 |
Other References:
CHU TING-TING; GAO NA; LI QIAN-QIAN; CHEN PU-GUANG; YANG XI-FEI; CHEN YONG-XIANG; ZHAO YU-FEN; LI YAN-MEI: "Specific Knockdown of Endogenous Tau Protein by Peptide-Directed Ubiquitin-Proteasome Degradation", CELL CHEMICAL BIOLOGY , ELSEVIER, AMSTERDAM, NL, vol. 23, no. 4, 21 April 2016 (2016-04-21), AMSTERDAM, NL , pages 453 - 461, XP029518406, ISSN: 2451-9456, DOI: 10.1016/j.chembiol.2016.02.016
QU JING; REN XIAOXI; XUE FENQIN; HE YI; ZHANG RUIHUA; ZHENG YAN; HUANG HAIXIA; WANG WEI; ZHANG JIANLIANG: "Specific Knockdown of alpha-Synuclein by Peptide-Directed Proteasome Degradation Rescued Its Associated Neurotoxicity", CELL CHEMICAL BIOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 27, no. 6, 30 April 2020 (2020-04-30), AMSTERDAM, NL , pages 751, XP086196119, ISSN: 2451-9456, DOI: 10.1016/j.chembiol.2020.03.010
CHEN SHUPING, YANG HAN, JIANG JINLU, YU SIYUAN, LI TINGDONG, GE SHENGXIANG: "Advances of targeted protein degradation technology and its applications in diseases therapy", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 37, no. 11, 25 November 2021 (2021-11-25), pages 3915 - 3932, XP093017945, DOI: 10.13345/j.cjb.200816
LIANG QIWEN, RAO BEN-QIANG, XU XI, XIAO GANG, WANG JIANPING: "Screening of tissue factor targeting peptides alternately from phage dis- play peptide library", CHINESE JOURNAL OF PATHOPHYSIOLOGY, ZHONGGUO BINGLI SHENGLI XUEHUI, BEIJING, CN, vol. 27, no. 12, 31 December 2011 (2011-12-31), CN , pages 2429 - 2432, XP093017947, ISSN: 1000-4718, DOI: 10.3969/j.issn.1000-4718.201.12.038
QU JING; REN XIAOXI; XUE FENQIN; HE YI; ZHANG RUIHUA; ZHENG YAN; HUANG HAIXIA; WANG WEI; ZHANG JIANLIANG: "Specific Knockdown of alpha-Synuclein by Peptide-Directed Proteasome Degradation Rescued Its Associated Neurotoxicity", CELL CHEMICAL BIOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 27, no. 6, 30 April 2020 (2020-04-30), AMSTERDAM, NL , pages 751, XP086196119, ISSN: 2451-9456, DOI: 10.1016/j.chembiol.2020.03.010
CHEN SHUPING, YANG HAN, JIANG JINLU, YU SIYUAN, LI TINGDONG, GE SHENGXIANG: "Advances of targeted protein degradation technology and its applications in diseases therapy", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 37, no. 11, 25 November 2021 (2021-11-25), pages 3915 - 3932, XP093017945, DOI: 10.13345/j.cjb.200816
LIANG QIWEN, RAO BEN-QIANG, XU XI, XIAO GANG, WANG JIANPING: "Screening of tissue factor targeting peptides alternately from phage dis- play peptide library", CHINESE JOURNAL OF PATHOPHYSIOLOGY, ZHONGGUO BINGLI SHENGLI XUEHUI, BEIJING, CN, vol. 27, no. 12, 31 December 2011 (2011-12-31), CN , pages 2429 - 2432, XP093017947, ISSN: 1000-4718, DOI: 10.3969/j.issn.1000-4718.201.12.038
Attorney, Agent or Firm:
BEIJING ZHI CHENG HAN FANG IP AGENCY CO., LTD. (CN)
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