Title:
TESTING FOR INFESTATION OF RAPESEED AND OTHER CRUCIFERAE BY THE FUNGUS LEPTOSPHAERIA MACULANS (BLACKLEG INFESTATION)
Document Type and Number:
WIPO Patent Application WO1994021788
Kind Code:
A3
Abstract:
PCT No. PCT/CA94/00130 Sec. 371 Date Jun. 5, 1997 Sec. 102(e) Date Jun. 5, 1997 PCT Filed Mar. 14, 1994 PCT Pub. No. WO94/21788 PCT Pub. Date Sep. 29, 1994A method of testing for infestation of tissue of rape or other Cruciferea with a virulent strain of Leptosphaeria maculans. The method involves isolating DNA of L. maculans from the tissue; subjecting the isolated DNA to amplification by the polymerase chain reaction (PCR) using sets of primers derived from LMR1 [SEQ ID NO:11] (Genbank accession number M77515), a repetitive element of L. maculans specific to virulent strains of the fungus, to form a product containing amplified L. maculans DNA; preferably separating the amplified L. maculans DNA from the product; and detecting the presence of the amplified separated L. maculans DNA. The invention also relates to a method of deriving DNA of L. maculans from plant tissue for amplification by PCR, to the oligonucleotides forming the primers used in the PCR and to a diagnostic test kit which makes use of the method of testing.
Inventors:
TAYLOR JANET L (CA)
Application Number:
PCT/CA1994/000130
Publication Date:
December 22, 1994
Filing Date:
March 14, 1994
Export Citation:
Assignee:
CA NAT RESEARCH COUNCIL (CA)
TAYLOR JANET L (CA)
TAYLOR JANET L (CA)
International Classes:
C07K14/37; (IPC1-7): C12N15/10; C12Q1/68; C07H21/04
Foreign References:
| DE4127862A1 | 1993-02-25 |
Other References:
W. MEYER ET AL.: "DNA- and PCR-fingerprinting in fungi", EXPERIMENTIA SUPPLEMENTUM (EXS), vol. 67, 1993, BIRKENHäUSER VERLAG, BASEL, SWITZERLAND, pages 311 - 320
B. XUE ET AL.: "Pathotype identification of Leptosphaeria maculans with PCR and oligonucleotide primers from ribosomal internal transcribed spacer sequences", PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, vol. 41, 1992, ACADEMIC PRESS LIMITED, NY,US;, pages 179 - 188
J.L. TAYLOR: "A simple, sensitive, and rapid method for detecting seed contaminated with highly virulent Leptoshaeria maculans", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 59, no. 11, November 1993 (1993-11-01), AM.SOC.MICROBIOL.,WASHINGTON,DC,US;, pages 3681 - 3685
J.L. TAYLOR AND I.E. BORGMANN: "An unusual repetitive element from highly virulent isolates of Leptosphaeria maculans and evidence of its transfer to a weakly virulent isolate", MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 7, no. 2, 1994, NATIONAL RESEARCH COUNCIL OF CANADA, CANADA;, pages 181 - 188
B. XUE ET AL.: "Pathotype identification of Leptosphaeria maculans with PCR and oligonucleotide primers from ribosomal internal transcribed spacer sequences", PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, vol. 41, 1992, ACADEMIC PRESS LIMITED, NY,US;, pages 179 - 188
J.L. TAYLOR: "A simple, sensitive, and rapid method for detecting seed contaminated with highly virulent Leptoshaeria maculans", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 59, no. 11, November 1993 (1993-11-01), AM.SOC.MICROBIOL.,WASHINGTON,DC,US;, pages 3681 - 3685
J.L. TAYLOR AND I.E. BORGMANN: "An unusual repetitive element from highly virulent isolates of Leptosphaeria maculans and evidence of its transfer to a weakly virulent isolate", MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 7, no. 2, 1994, NATIONAL RESEARCH COUNCIL OF CANADA, CANADA;, pages 181 - 188
Download PDF:
Previous Patent: METHOD FOR DEFINED DELETIONS OF DNA
Next Patent: EXPRESSION OF HETEROLOGOUS POLYPEPTIDES IN HALOBACTERIA
Next Patent: EXPRESSION OF HETEROLOGOUS POLYPEPTIDES IN HALOBACTERIA