Title:
TET-ASSISTED PYRIDINE BORANE SEQUENCING
Document Type and Number:
WIPO Patent Application WO/2024/076981
Kind Code:
A3
Abstract:
Methods of amplifying libraries after introduction of dihydrouracil (DHU) residues by methods such as TET-assisted Pyridine Borane Sequencing (TAPS), and variants of TAPS including TAPS with blocking by β-glucosylation (TAPSβ) and Chemically-assisted Pyridine Borane Sequencing (CAPS) are described. The methods comprise introducing DHU residues into a nucleic acid sample and preparation of a sequencing library by a complementary strand synthesis step reaction with a first polymerase or polymerase mixture that is tolerant of DHU residues and/or products resulting from the introduction of the DHU residues and/or the TAPS process followed by exponential amplification. Improved methods for conversion of oxidized nucleotide residues to DHU are also described.
Inventors:
MILLER BRONWEN (US)
WILSON ROSEMARY (US)
TOSTI LUCA (US)
VACCARO ABRAM (US)
SONG CHUNXIAO (US)
WALSH SARAH (US)
WILSON ROSEMARY (US)
TOSTI LUCA (US)
VACCARO ABRAM (US)
SONG CHUNXIAO (US)
WALSH SARAH (US)
Application Number:
PCT/US2023/075823
Publication Date:
May 16, 2024
Filing Date:
October 03, 2023
Export Citation:
Assignee:
EXACT SCIENCES INNOVATION LTD (GB)
International Classes:
C12Q1/68; C07H21/00; C12N9/12; C12Q1/6869
Foreign References:
| US20200024643A1 | 2020-01-23 | |||
| US20220275424A1 | 2022-09-01 |
Other References:
POTAPOV VLADIMIR, ONG JENNIFER L.: "Examining Sources of Error in PCR by Single-Molecule Sequencing", PLOS ONE, vol. 12, no. 1, 6 January 2017 (2017-01-06), pages e0169774, XP055800227, DOI: 10.1371/journal.pone.0169774
ZHENG NAN; YIN LICHEN; SONG ZIYUAN; MA LIANG; TANG HAOYU; GABRIELSON NATHAN P.; LU HUA; CHENG JIANJUN: "Maximizing gene delivery efficiencies of cationic helical polypeptides via balanced membrane penetration and cellular targeting", BIOMATERIALS, ELSEVIER, AMSTERDAM, NL, vol. 35, no. 4, 7 November 2013 (2013-11-07), AMSTERDAM, NL , pages 1302 - 1314, XP028787913, ISSN: 0142-9612, DOI: 10.1016/j.biomaterials.2013.09.090
ZHENG NAN; YIN LICHEN; SONG ZIYUAN; MA LIANG; TANG HAOYU; GABRIELSON NATHAN P.; LU HUA; CHENG JIANJUN: "Maximizing gene delivery efficiencies of cationic helical polypeptides via balanced membrane penetration and cellular targeting", BIOMATERIALS, ELSEVIER, AMSTERDAM, NL, vol. 35, no. 4, 7 November 2013 (2013-11-07), AMSTERDAM, NL , pages 1302 - 1314, XP028787913, ISSN: 0142-9612, DOI: 10.1016/j.biomaterials.2013.09.090
Attorney, Agent or Firm:
JONES, J., Mitchell (US)
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