Background Art The amide derivatives of the present invention are antagonists of NMDA (N-methyl-D- aspartate) NR2B receptor, and have a number of therapeutic applications, particularly in the treatment of pain, stroke, traumatic brain injury, Parkinson's disease, Alzheimer's disease, depression, anxiety, migraine, or the like.

Glutamate plays a dual role in the central nervous system (CNS) as essential amino acid and the principal excitatory neurotransmitters. There are two major class of receptors, ionotoropic and metabotropic. Ionotropic receptors are classified into three major subclass, N-methyl-asparatate (NMDA), 2-amino-3 (methyl-3-hydroxyisoxazol-4-yl) propionic acid (AMPA) and kainate. There is considerable preclinical evidence that hyperalgesia and allodynia following peripheral tissue or nerve injury is not only due to an increase in the sensitivity of primary afferent nociceptors at the site of injury but also depends on NMDA receptor-mediated central changes in synaptic excitability. In humans, NMDA receptor antagonists have also been found to decrease both pain perception and sensitization. Also, overactivation of the NMDA receptor is a key event for triggering neuronal cell death under pathological conditions of acute and chronic forms of neurodegeneration. However, while NMDA receptor inhibition has therapeutic utility in the treatment of pain and neurodegenerative diseases, there are significant liabilities to many available NMDA receptor antagonists that can cause potentially serious side effects. NMDA subunits are differentially distributed in the CNS. Especially, NR2B is believed to be restricted to the forebrain and laminas I and II of the dosal horn. The more discrete distribution of NR2B subunit in the CNS may support a reduced side-effect profile of agents that act selectively at this site. For example, NMDA NR2B selective antagonists may have clinical utility for the treatment of neuropathic and other pain conditions in human with a reduced side-effect profile than existing NMDA antagonists (S. Boyce, et al. , Neuropharmacology, 38, pp. 611- 623 (1999)).

International Patent Application Number (WO) 0208928 discloses a variety of benzamide compounds, which are NMDA NR2B antagonists, for example, compound (i) below: Compound (i) shows an IC50 of <3mcM at HERG potassium channel.

W09967203 describes cyclohexyl derivatives which are claimed to be useful in the treatment of pain.

There is a need to provide new NMDA NR2B antagonists that are good drug candidates.

In particular, preferred compounds should bind potently to the NR2B receptor and show functional activity as antagonists whilst showing little affinity for other receptors. They should be well absorbed from the gastrointestinal tract, be metabolically stable and possess favourable pharmacokinetic properties. They should be non-toxic and demonstrate few side-effects.

Furthermore, the ideal drug candidate will exist in a physical form that is stable, non- hygroscopic and easily formulated.

In particular, it would be desirable to provide a NMDA NR2B selective antagonist with reduced inhibitory activity at HERG potassium channel.

Detailed Description of the Invention The invention, therefore, provides a compound of the formula (I): or a pharmaceutically acceptable salt or solvate thereof, wherein: A and B independently represent CH2 or 0, with the proviso that A and B are not simultaneously 0 ; Cy represents one of the following optionally substituted by one to three groups selected from hydroxy, halogen, Cl 6alkyl, C 6alkoxy, C1-6haloalkyl, C1-6alkylamino and amino; R'and R 2 are independently selected from hydroxy, halogen, C1-6alkyl, C1-6alkoxy, C1- 6haloalkyl and C3-8 cycloalkyl ; n represents an integer from 0-4; X is hydrogen, hydroxy, halogen or Cl-6 alkoxy ; Y is oxy, thio, a 1-4 membered alkylene, a 2-4 membered alkylene ether, 2-4 membered alkylene thioether or an oxyethyleneoxy group, optionally substituted by 1 to 4 groups independently selected from hydroxy, halogen, Cl 6alkyl, Cl 6alkoxy and Cl 6haloalkyl ; Z is CH or N; and p represents an integer from 0-5 when Z is CH or 0-4 when Z is N, when p represents 2 or more, two of R2s may be taken together with the carbon atoms to which they are attached to form a 5-8 membered cycloalkyl ring.

In the above definitions, halo means fluoro, chloro, bromo or iodo. Alkyl, alkylene, and alkoxy groups, containing the requisite number of carbon atoms, can be unbranched or branched. Examples of alkyl include methyl, ethyl, n-propyl, i-propyl, n-butyl, i-butyl, sec- butyl and t-butyl. Examples of cycloalkyl include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and cyclooctyl. Examples of alkylene include methylene, ethylene, n-propylene, 1-methylethylene, n-butylene, 1-methylpropylene, 2-methylpropylene and 1, 1- dimethylethylene. Examples of alkoxy include methoxy, ethoxy, n-propoxy, i-propoxy, n- butoxy, i-butoxy, sec-butoxy and t-butoxy. Haloalkyl defines an alkyl group substituted by one or more halogen groups. Examples of haloalkyl include difluoromethyl, trifluoromethyl and pentafluoroethyl. 2-4 membered alkylene ether difines a 2 to 4 membered chain wherein one member is oxygen and at least one ther member is Cl-C3 alkylene. Examples of 2-4 membered alkylene ether groups include oxymethylene, methyleneoxy, ethyleneoxy, oxyethylene and methyleneoxymethylene. Examples of 2-4 membered alkylene thioether groups include thiomethylene, methylenethio, ethylenethio and thioethylene. Examples of 5-8 membered cycloalkyl rings include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and cyclooctyl.

In a preferred aspect (A), the invention provides a compound of the formula (I), or a pharmaceutically acceptable salt or solvate thereof, wherein Cy is selected from 4- hydroxyphenyl, lH-pyrazol-4-yl, 2-oxo-2, 3-dihydro-1, 3-benzoxazole-6-yl, 2-hydroxy-4- pyridyl, 5-pyrazolyl, 3-pyrazolyl, 4-pyrazolyl, 2-oxoindoline, 3-amino-4-pyrazolyl and 2- hydroxy-5-pyridyl, unsubstituted or substititued by halogen, e. g. fluoro or Cl 6alkyl, e. g methyl, more preferably 4-hydroxyphenyl unsubstituted or substititued by fluoro, most preferably substituted by fluoro ortho to the phenolic hydroxy group, and A, B, Rl, R2, n, p, X, Y and Z are as defined above.

In a further preferred aspect (B), the invention provides a compound of the formula (I), or a pharmaceutically acceptable salt or solvate thereof, wherein Cy is defined above, either in its broadest aspect or in a preferred, more or most preferred aspect under (A), n is 0 and A, B, Rl, R2, p, X, Y and Z are as defined above.

In a further preferred aspect (C), the invention provides a compound of the formula (I), or a pharmaceutically acceptable salt or solvate thereof, wherein Cy is defined above, either in its broadest aspect or in a preferred, more or most preferred aspect under (A) or (B), n is defined above, either in the broadest aspect or in a preferred aspect under (B), p is 0-2 and R2 is selected from fluoro, chloro, Cl. 6alkyl, e. g. methyl, ethyl, isopropyl or n-propyl, methoxy or trifluoromethyl, more preferably methoxy, chloro, fluoro and methyl, and A, B, Rl, X, Y and Z are as defined above.

In a further preferred aspect (D), the invention provides a compound of the formula (I), or a pharmaceutically acceptable salt or solvate thereof, wherein Cy is defined above, either in its broadest aspect or in a preferred, more or most preferred aspect under (A), (B) or (C), n is defined above, either in the broadest aspect or in a preferred aspect under (B) or (C), p and R2 are defined above, either in the broadest aspect or in a preferred or more preferred aspect under (C), X is hydrogen, fluoro, hydroxy or methoxy, more preferably hydrogen or hydroxy, and A, B, Rl, Y and Z are as defined above.

In a further preferred aspect (E), the invention provides a compound of the formula (I), or a pharmaceutically acceptable salt or solvate thereof, wherein Cy is defined above, either in its broadest aspect or in a preferred, more or most preferred aspect under (A), (B) or (C) or (D), n is defined above, either in the broadest aspect or in a preferred aspect under (B), (C) or (D), p and R2 are defined above, either in the broadest aspect or in a preferred or more preferred aspect under (C) or (D), X is defined above, either in its broadest aspect or in a preferred or more preferred aspect under (D), Y is methylene, oxyethyleneoxy, oxymethylene, methyleneoxy, methyleneoxymethylene, ethyleneoxy, oxyethylene or oxy, more preferably methyleneoxy, and A, B, RI, and Z are as defined above.

In a further preferred aspect (F), the invention provides a compound of the formula (I), or a pharmaceutically acceptable salt or solvate thereof, wherein Cy is defined above, either in its broadest aspect or in a preferred, more or most preferred aspect under (A), (B), (C), (D) or (E), n is defined above, either in the broadest aspect or in a preferred aspect under (B), (C), (D) or (E), p and R2 are defined above, either in the broadest aspect or in a preferred or more preferred aspect under (C), (D) or (E), X is defined above, either in its broadest aspect or in a preferred or more preferred aspect under (D) or (E), Y is defined above, either in its broadest aspect or in a preferred or more preferred aspect under (E), Z is C and A, B and are as defined above.

In a further preferred aspect (G), the invention provides a compound of the formula (I), or a pharmaceutically acceptable salt or solvate thereof, wherein Cy is defined above, either in its broadest aspect or in a preferred, more or most preferred aspect under (A), (B), (C), (D), (E) or (F), n is defined above, either in the broadest aspect or in a preferred aspect under (B), (C), (D), (E) or (F), p and R2 are defined above, either in the broadest aspect or in a preferred or more preferred aspect under (C), (D), (E) or (F), X is defined above, either in its broadest aspect or in a preferred or more preferred aspect under (D), (E) or (F), Y is defined above, either in its broadest aspect or in a preferred or more preferred aspect under (E) or (F), Z is defined above, either in its broadest aspect or in a preferred aspect under (F), the group Y is para located to and in a trans configuration to X, and A, B and R'are as defined above.

Individual preferred A, B, Cy, Rl, R2, n, p, X, Y and Z groups are those defined by the A, B, Cy, RI, R2, n, p, X, Y and Z groups in the Examples section below.

Particularly preferred compounds of the invention include those in which each variable in Formula (I) is selected from the preferred groups for each variable. Even more preferable compounds of the invention include those where each variable in Formula (I) is selected from the more or most preferred groups for each variable.

A specific compound according to the invention is selected from the list consisting of : 4-Hydroxy-N { [cis-4- (phenoxymethyl) cyclohexyl] methyl} benzamide ; 4-Hydroxy-N- ( {cis-4- [ (4-methoxyphenoxy) methyl] cyclohexyl} methyl) benzamide; N- { [cis-4-(Benzyloxy) cyclohexyl] methyl}-4-hydroxybenzamide ; N-({cis-4-[(4-Chlorobenzyl) oxy] cyclohexyl} methyl)-4-hydroxybenzamide ; N ( {cis-4- [ (3-Chlorobenzyl) oxy] cyclohexyl} methyl)-4-hydroxybenzamide ; 4-Hydroxy-N { [cis-4- (4-methoxyphenoxy) cyclohexyl] methyl} benzamide; N- { [cis-4- (4-Chlorophenoxy) cyclohexyl] methyl}-4-hydroxybenzamide ; 4-Hydroxy-N-{ [1-hydroxy-4-(phenoxymethyl) cyclohexyl] methyl} benzamide ; N-({trans-4-[(4-Fluorophenoxy) methyl]-1-hydroxycyclohexyl} methyl)-4- hydroxybenzamide ; N- ({trans-4-[(3-Fluorophenoxy)methyl]-1-hydroxycyclohexyl}meth yl)-4- hydroxybenzamide ; N-({trans-4-[(2-Fluorophenoxy)methyl]-1-hydroxycyclohexyl}me thyl)-4- hydroxybenzamide ; N-({trans-4-[(2, 6-Difluorophenoxy) methyl]-1-hydroxycyclohexyl} methyl)-4- hydroxybenzamide ; N-({trans-4-[(3,5-Difluorophenoxy)methyl]-1-hydroxycyclohexy l)methyl)-4- hydroxybenzamide ; N ( {trans-4- [ (3-Chlorophenoxy) methyl]-1-hydroxycyclohexyl} methyl)-4- hydroxybenzamide; N-( {trans-4- [ (4-Chlorophenoxy) methyl]-1-hydroxycyclohexyl} methyl)-4- hydroxybenzamide; 4-Hydroxy-N-( {trans-1-hydroxy-4-[(2- methylphenoxy) methyl] cyclohexyl} methyl) benzamide; 4-Hydroxy-N-({trans-1-hydroxy-4-[(3- methylphenoxy) methyl] cyclohexyl} methyl) benzamide; 4-Hydroxy-N ( {trans-1-hydroxy-4- [ (4- methylphenoxy) methyl] cyclohexyl} methyl) benzamide; N-( {trans-4-[(Benzyloxy) methyl]-1-hydroxycyclohexyl} methyl) -4-hydroxybenzamide; N-[(trans-4-{[(2-Fluorobenzyl) oxy] methyl}-1-hydroxycyclohexyl) methyl-4- hydroxybenzamide; N-[(trans-4-{[(4-Fluorobenzyl) oxy] methyl}-1-hydroxycyclohexyl) methyl] -4- hydroxybenzamide ; 4-Hydroxy-N-{ [trans-1-hydroxy-4- (2-phenoxyethyl) cyclohexyl] methyl} benzamide ; N-({trans-4-[2-(2-Fluorophenoxy)ethyl]-1-hydroxycyclohexyl} methyl) -4- hydroxybenzamide; N-( {trans-4- [2- (3-Fluorophenoxy) ethyl]-1-hydroxycyclohexyl} methyl) -4- hydroxybenzamide; N ( {trans-4- [2- (4-Fluorophenoxy) ethyl]-1-hydroxycyclohexyl} methyl)-4- hydroxybenzamide; N { [trans-4- (Benzyloxy)-1-hydroxycyclohexyl] methyl}-4-hydroxybenzamide ; N-f [trans-4- (4-Chlorophenoxy)-1-hydroxycyclohexyl] methyl}-4-hydroxybenzamide ; N-{ [cis-4- (4-Chlorophenoxy)-1-hydroxycyclohexyl] methyl}-4-hydroxybenzamide ; N-{ [trans-4- (4-Chlorophenoxy)-1-hydroxycyclohexyl] methyl}-3-fluoro-4- hydroxybenzamide; N-{ [cis-4- (4-Chlorophenoxy)-1-hydroxycyclohexyl] methyl}-3-fluoro-4-hydroxybenzamide ; (+)-4-hydroxy-N-{ [SS- (phenoxymethyl) tetrahydro-2H-pyran-2S-yl] methyl} benzamide; (-)-4-hydroxy-N { [SR- (phenoxymethyl) tetrahydro-2H-pyran-2R-yl] methyl} benzamide ; 4-hydroxy-N-{ [5S- (benzyloxymethyl) tetrahydro-2H-pyran-2S-yl] methyl} benzamide; 4-hydroxy-N-{ [SR-(benzyloxymethyl) tetrahydro-2H-pyran-2R-yl] methyl} benzamide; (-)-4-Hydroxy-N-{[(3R,6S)-6-(phenoxymethyl)tetrahydro-2H-pyr an-3- yl] methyl} benzamide ; (+)-4-Hydroxy-N-{ [ (3S, 6R)-6- (phenoxymethyl) tetrahydro-2H-pyran-3- yl] methyl} benzamide ; N-({trans-4-[(2-Fluorobenzyl)oxy]-1-hydroxycyclohexyl) methyl) -4-hydroxybenzamide; 3-Fluoro-N-({trans-4-[2-(2-fluorophenoxy)ethyl]-1-hydroxycyc lohexyl}methyl)-4- hydroxybenzamide; trans-N { [4- (4-Chlorophenoxy) cyclohexyl] methyl}-3-fluoro-4-hydroxybenzamide ; cis-N- { [4- (4-Chlorophenoxy) cyclohexyl] methyl}-3-fluoro-4-hydroxybenzamide ; N-{ [cis-4- (4-Fluorophenoxy) cyclohexyl] methyl}-4-hydroxybenzamide ; 3-Fluoro-N { [cis-4- (4-fluorophenoxy) cyclohexyl] methyl}-4-hydroxybenzamide ; N-({trans-4-[2-(2-Fluorophenoxy)ethyl]-1-hydroxycyclohexyl}m ethyl)-1H-pyrazole-4- carboxamide; 4-Hydroxy-N-{ [cis-4- (2-phenylethoxy) cyclohexyl] methyl} benzamide ; 2-Fluoro-4-hydroxy-N- { [trans-1-hydroxy-4- (phenoxymethyl) cyclohexyl] methyl} benzamide; N-({trans-4-[(Benzyloxy)methyl]-1-hydroxycyclohexyl} methyl) -3-fluoro-4- hydoxybenzamide; N-({cis-4-[(Benzyloxy)methyl]cyclohexyl}methyl)-4-hydroxyben zamide 3-Fluoro-4-hydroxy-N { [trans-1-hydroxy-4- (phenoxymethyl) cyclohexyl] methyl} benzamide; 3-Fluoro-4-hydroxy-N-{[trans-1-hydroxy-4-(2- phenoxyethyl) cyclohexyl] methyl} benzamide ; <BR> <BR> 3-Fluoro-N-[(trans-4-{ [(4-fluorobenzyl) oxy] methyl}-1-hydroxycyclohexyl) methyl]-4- hydroxybenzamide; 3-Fluoro-N-({trans-4-[(2-fluorophenoxy)methyl]-1-hydroxycycl ohexyl}methyl)-4- hydroxybenzamide; 3-Fluoro-N-({trans-4-[(4-fluorophenoxy) methyl]-1-hydroxycyclohexyl} methyl)-4- hydroxybenzamide ; 4-Hydroxy-N-[(trans-1-hydroxy-4-{[(5-methylpyridin-2- yl) oxy] methyl} cyclohexyl) methyl] benzamide ; N- [ (trans-4-Benzyl-l-hydroxycyclohexyl) methyl]-4-hydroxybenzamide ; 3-Fluoro-N-[(trans-4-{[(2-fluorobenzyl) oxy] methyl}-1-hydroxycyclohexyl) methyl]-4- hydroxybenzamide ; 6-Hydroxy-N- { [cis-4-(2-phenethoxy) cyclohexyl] methyl} nicotinamide ; N- { [cis-4-(2-Phenyletoxy)cyclohexyl]methyl}-1H-pyrazole-4-carbo xamide ; N-{ [cis-4- (Phenoxymethyl) cyclohexyl] methyl}-lH-pyrazole-4-carboxamide ; N-{ [cis-4- (2-Phenoxyethyl) cyclohexyl] methyl}-lH-pyrazole-4-carboxamide ; N-({cis-4-[(3-Fluorophenoxy) methyl] cyclohexyl} methyl)-lH-pyrazole-4-carboxamide ; N ( {cis-4- [ (4-Fluorophenoxy) methyl] cyclohexyl} methyl)-lH-pyrazole-4-carboxamide ; N-({ (2R, 5R)-5-[(4-Fluorophenoxy) methyl] tetrahydro-2H-pyran-2-yl} methyl)-lH-pyrazole- 4-carboxamide; N { [cis-4- (4-Methoxybenzyl) cyclohexyl] methyl}-1 H-pyrazole-4-carboxamide ; 3-Amino-N-[(cis-4-benzylcclohexyl)methyl]-1H-pyrazole-4-carb oxamide ; <BR> <BR> N-({ (2R, 5R)-5-[(4-Chlorophenoxy) methyl] tetrahydro-2H-pyran-2-yl} methyl)-lH-pyrazole- 4-carboxamide; <BR> <BR> 3-Amino-N-({ (2R, SR)-5-[(4-fluorophenoxy) methyl] tetrahydro-2H-pyran-2-yl} methyl)-1H- pyrazole-4-carboxamide; 3-Amino-N-({ (2R, 5R)-5-[(4-chlorophenoxy)methyl]tetrahydro-2H-pyran-2-yl}meth yl)-1H- pyrazole-4-carboxamide; and <BR> <BR> 3-Amino-N-({ (2R, 5R)-5-[(4-ethylphenoxy) methyl] tetrahydro-2H-pyran-2-yl} methyl)-1H- pyrazole-4-carboxamide; or a pharmaceutically acceptable salt or solvate thereof.

A suitable sub-formula of compounds of formula (I) may be represented by formula (Ia) or a pharmaceutically acceptable salt or solvate thereof, wherein: RiA, R2A or R3A are independently selected from hydrogen, halogen, C1-6alkyl, C1-6alkoxy, C1-6haloalkyl or C3-8 cycloalkyl ; XA is hydrogen or hydroxy; YA is oxy, a 1-4 membered alkylene group, a 2-4 membered alkylene ether group or an oxyethyleneoxy group; and ZA is C or N.

Pharmaceutically acceptable salts of the compounds of formula (I) include the base salts thereof. Suitable base salts are formed from bases which form non-toxic salts. Examples include the aluminium, arginine, benzathine, calcium, choline, diethylamine, diolamine, glycine, lysine, magnesium, meglumine, olamine, potassium, sodium, tromethamine and zinc salts. For a review on suitable salts, see"Handbook of Pharmaceutical Salts: Properties, Selection, and Use"by Stahl and Wermuth (Wiley-VCH, Weinheim, Germany, 2002).

A pharmaceutically acceptable salt of a compound of formula (I) may be readily prepared by mixing together solutions of the compound of formula (I) and the desired base, as appropriate. The salt may precipitate from solution and be collected by filtration or may be recovered by evaporation of the solvent. The degree of ionisation in the salt may vary from completely ionised to almost non-ionised.

The compounds of the invention may exist in both unsolvated and solvated forms. The term'solvate'is used herein to describe a molecular complex comprising the compound of the invention and one or more pharmaceutically acceptable solvent molecules, for example, ethanol. The term'hydrate'is employed when said solvent is water. f Included within the scope of the invention are complexes such as clathrates, drug-host inclusion complexes wherein, in contrast to the aforementioned solvates, the drug and host are present in stoichiometric or non-stoichiometric amounts. Also included are complexes of the drug containing two or more organic and/or inorganic components, which may be in stoichiometric or non-stoichiometric amounts. The resulting complexes may be ionised, partially ionised, or non-ionised. For a review of such complexes, see J Pharm Sci, 64 (8), 1269-1288 by Haleblian (August 1975).

Hereinafter all references to compounds of formula (I) include references to salts, solvates and complexes thereof and to solvates and complexes of salts thereof.

The compounds of the invention include compounds of formula (I) as hereinbefore defined, polymorphs, prodrugs, and isomers thereof (including optical, geometric and tautomeric isomers) as hereinafter defined and isotopically-labeled compounds of formula (I).

As stated, the invention includes all polymorphs of the compounds of formula (1) as hereinbefore defined.

Also within the scope of the invention are so-called'prodrugs'of the compounds of formula (1). Thus certain derivatives of compounds of formula (I) which may have little or no pharmacological activity themselves can, when administered into or onto the body, be converted into compounds of formula (I) having the desired activity, for example, by hydrolytic cleavage. Such derivatives are referred to as'prodrugs'. Further information on the use of prodrugs may be found in'Pro-drugs as Novel Delivery Systems, Vol. 14, ACS Symposium Series (T Higuchi and W Stella) and'Bioreversible Carriers in Drug Design', Pergamon Press, 1987 (ed. E B Roche, American Pharmaceutical Association).

Prodrugs in accordance with the invention can, for example, be produced by replacing appropriate functionalities present in the compounds of formula (I) with certain moieties known to those skilled in the art as'pro-moieties'as described, for example, in"Design of Prodrugs"by H Bundgaard (Elsevier, 1985).

Some examples of prodrugs in accordance with the invention include: (i) where the compound of formula (I) contains an alcohol functionality (-OH), an ether thereof, for example, replacement of the hydrogen with (C-C6) alkanoyloxymethyl ; and (ii) where the compound of formula (I) contains a primary or a secondary amino functionality (NHR where R H), an amide thereof, for example, replacement of one or both hydrogens with (CI-Clo) alkanoyl.

Further examples of replacement groups in accordance with the foregoing examples and examples of other prodrug types may be found in the aforementioned references.

Compounds of formula (I) containing one or more asymmetric carbon atoms can exist as two or more stereoisomers. Where a compound of formula (I) contains an alkenyl or alkenylene group, geometric cisltrans (or Z/E) isomers are possible. Where the compound contains, for example, a keto or oxime group or an aromatic moiety, tautomeric isomerism ('tautomerism') can occur. It follows that a single compound may exhibit more than one type of isomerism.

Included within the scope of the present invention are all stereoisomers, geometric isomers and tautomeric forms of the compounds of formula (I), including compounds exhibiting more than one type of isomerism, and mixtures of one or more thereof.

Cisltrans isomers may be separated by conventional techniques well known to those skilled in the art, for example, chromatography and fractional crystallisation.

Conventional techniques for the preparation/isolation of individual enantiomers include chiral synthesis from a suitable optically pure precursor or resolution of the racemate (or the racemate of a salt or derivative) using, for example, chiral high pressure liquid chromatography (HPLC).

Alternatively, the racemate (or a racemic precursor) may be reacted with a suitable optically active compound, for example, an alcohol, or, in the case where the compound of formula (I) contains an acidic moiety, a suitable base. The resulting diastereomeric mixture may be separated by chromatography and/or fractional crystallization and one or both of the diastereoisomers converted to the corresponding pure enantiomer (s) by means well known to a skilled person.

Chiral compounds of the invention (and chiral precursors thereof) may be obtained in enantiomerically-enriched form using chromatography, typically HPLC, on an asymmetric resin with a mobile phase consisting of a hydrocarbon, typically heptane or hexane, containing from 0 to 50% isopropanol, typically from 2 to 20%, and from 0 to 5% of an alkylamine, typically 0. 1% diethylamine. Concentration of the eluate affords the enriched mixture.

Stereoisomeric conglomerates may be separated by conventional techniques known to those skilled in the art-see, for example, "Stereochemistry of Organic Compounds"by E L Eliel (Wiley, New York, 1994).

The present invention includes all pharmaceutically acceptable isotopically-labelled compounds of formula (I) wherein one or more atoms are replaced by atoms having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number usually found in nature.

Examples of isotopes suitable for inclusion in the compounds of the invention include isotopes of hydrogen, such as 2H and 3H, carbon, such as IIC, 13C and 14C, chlorine, such as 36CI, fluorine, such as 18F, iodine, such as 123I and 125I, nitrogen, such as'3N and 15N, oxygen, such as 110, 17 0 and 180, phosphorus, such as 32p, and sulphur, such as 35S.

Certain isotopically-labelled compounds of formula (I), for example, those incorporating a radioactive isotope, are useful in drug and/or substrate tissue distribution studies. The radioactive isotopes tritium, i. e. 3H, and carbon-14, i. e. 14C, are particularly useful for this purpose in view of their ease of incorporation and ready means of detection.

Substitution with heavier isotopes such as deuterium, i. e. 2H, may afford certain therapeutic advantages resulting from greater metabolic stability, for example, increased in vivo half-life or reduced dosage requirements, and hence may be preferred in some circumstances.

Substitution with positron emitting isotopes, such as 11C, 18F, 15O and 13N, can be useful in Positron Emission Topography (PET) studies for examining substrate receptor occupancy.

Isotopically-labeled compounds of formula (I) can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described in the accompanying Examples and Preparations using an appropriate isotopically-labeled reagents in place of the non-labeled reagent previously employed.

Pharmaceutically acceptable solvates in accordance with the invention include those wherein the solvent of crystallization may be isotopically substituted, e. g. D20, d6-acetone, d6-DMSO.

The compounds of the present invention are antagonists of NMDA (N-methyl-D- aspartate) NR2B receptor, and have a number of therapeutic applications, particularly in the treatment of pain, stroke, traumatic brain injury, Parkinson's disease, Alzheimer's disease, depression, anxiety, migraine, or the like.

The compounds of the present invention are useful for the general treatment of pain, particularly neuropathic pain. Physiological pain is an important protective mechanism designed to warn of danger from potentially injurious stimuli from the external environment.

The system operates through a specific set of primary sensory neurones and is exclusively activated by noxious stimuli via peripheral transducing mechanisms (Millan 1999 Prog.

Neurobio. 57: 1-164 for an integrative Review). These sensory fibres are known as nociceptors and are characterised by small diameter axons with slow conduction velocities.

Nociceptors encode the intensity, duration and quality of noxious stimulus and by virtue of their topographically organised projection to the spinal cord, the location of the stimulus.

The nociceptors are found on nociceptive nerve fibres of which there are two main types, A- delta fibres (myelinated) and C fibres (non-myelinated). The activity generated by nociceptor input is transferred after complex processing in the dorsal horn, either directly or via brain stem relay nuclei to the ventrobasal thalamus and then on to the cortex, where the sensation of pain is generated.

Intense acute pain and chronic pain may involve the same pathways driven by pathophysiological processes and as such cease to provide a protective mechanism and instead contribute to debilitating symptoms associated with a wide range of disease states.

Pain is a feature of many trauma and disease states. When a substantial injury, via disease or trauma, to body tissue occurs the characteristics of nociceptor activation are altered.

There is sensitisation in the periphery, locally around the injury and centrally where the nociceptors terminate. This leads to hypersensitivity at the site of damage and in nearby normal tissue. In acute pain these mechanisms can be useful and allow for the repair processes to take place and the hypersensitivity returns to normal once the injury has healed.

However, in many chronic pain states, the hypersensitivity far outlasts the healing process and is normally due to nervous system injury. This injury often leads to maladaptation of the afferent fibres (Woolf & Salter 2000 Science 288: 1765-1768). Clinical pain is present when discomfort and abnormal sensitivity feature among the patient's symptoms. Patients tend to be quite heterogeneous and may present with various pain symptoms. There are a number of typical pain subtypes: 1) spontaneous pain which may be dull, burning, or stabbing; 2) pain responses to noxious stimuli are exaggerated (hyperalgesia); 3) pain is produced by normally innocuous stimuli (allodynia) (Meyer et al. , 1994 Textbook of Pain 13-44). Although patients with back pain, arthritis pain, CNS trauma, or neuropathic pain may have similar symptoms, the underlying mechanisms are different and, therefore, may require different treatment strategies. Therefore pain can be divided into a number of different areas because of differing pathophysiology, these include nociceptive, inflammatory, neuropathic pain etc. It should be noted that some types of pain have multiple aetiologies and thus can be classified in more than one area, e. g. Back pain, Cancer pain have both nociceptive and neuropathic components.

Nociceptive pain is induced by tissue injury or by intense stimuli with the potential to cause injury. Pain afferents are activated by transduction of stimuli by nociceptors at the site of injury and sensitise the spinal cord at the level of their termination. This is then relayed up the spinal tracts to the brain where pain is perceived (Meyer et al. , 1994 Textbook of Pain 13-44). The activation of nociceptors activates two types of afferent nerve fibres. Myelinated A-delta fibres transmitted rapidly and are responsible for the sharp and stabbing pain sensations, whilst unmyelinated C fibres transmit at a slower rate and convey the dull or aching pain. Moderate to severe acute nociceptive pain is a prominent feature of, but is not limited to pain from strains/sprains, post-operative pain (pain following any type of surgical procedure), posttraumatic pain, burns, myocardial infarction, acute pancreatitis, and renal colic. Also cancer related acute pain syndromes commonly due to therapeutic interactions such as chemotherapy toxicity, immunotherapy, hormonal therapy and radiotherapy. Moderate to severe acute nociceptive pain is a prominent feature of, but is not limited to, cancer pain which may be tumour related pain, (e. g. bone pain, headache and facial pain, viscera pain) or associated with cancer therapy (e. g. postchemotherapy syndromes, chronic postsurgical pain syndromes, post radiation syndromes), back pain which may be due to herniated or ruptured intervertabral discs or abnormalities of the lumber facet joints, sacroiliac joints, paraspinal muscles or the posterior longitudinal ligament Neuropathic pain is defined as pain initiated or caused by a primary lesion or dysfunction in the nervous system (IASP definition). Nerve damage can be caused by trauma and disease and thus the term'neuropathic pain'encompasses many disorders with diverse aetiologies. These include but are not limited to, diabetic neuropathy, post herpetic neuralgia, back pain, cancer neuropathy, HIV neuropathy, Phantom limb pain, Carpal Tunnel Syndrome, chronic alcoholism, hypothyroidism, trigeminal neuralgia, uremia, or vitamin deficiencies. Neuropathic pain is pathological as it has no protective role. It is often present well after the original cause has dissipated, commonly lasting for years, significantly decreasing a patients quality of life (Woolf and Mannion 1999 Lancet 353: 1959-1964). The symptoms of neuropathic pain are difficult to treat, as they are often heterogeneous even between patients with the same disease (Woolf & Decosterd 1999 Pain Supp. 6: S141-S147 ; Woolf and Mannion 1999 Lancet 353: 1959-1964). They include spontaneous pain, which can be continuous, or paroxysmal and abnormal evoked pain, such as hyperalgesia (increased sensitivity to a noxious stimulus) and allodynia (sensitivity to a normally innocuous stimulus).

The inflammatory process is a complex series of biochemical and cellular events activated in response to tissue injury or the presence of foreign substances, which result in swelling and pain (Levine and Taiwo 1994: Textbook of Pain 45-56). Arthritic pain makes up the majority of the inflammatory pain population. Rheumatoid disease is one of the commonest chronic inflammatory conditions in developed countries and rheumatoid arthritis is a common cause of disability. The exact aetiology of RA is unknown, but current hypotheses suggest that both genetic and microbiological factors may be important (Grennan & Jayson 1994 Textbook of Pain 397-407). It has been estimated that almost 16 million Americans have symptomatic osteoarthritis (OA) or degenerative joint disease, most of whom are over 60 years of age, and this is expected to increase to 40 million as the age of the population increases, making this a public health problem of enormous magnitude (Houge & Mersfelder 2002 Ann Pharmacother. 36: 679-686 ; McCarthy et al. , 1994 Textbook of Pain 387-395). Most patients with OA seek medical attention because of pain.

Arthritis has a significant impact on psychosocial and physical function and is known to be the leading cause of disability in later life. Other types of inflammatory pain include but are not limited to inflammatory bowel diseases (IBD), Other types of pain include but are not limited to; -Musculo-skeletal disorders including but not limited to myalgia, fibromyalgia, spondylitis, sero-negative (non-rheumatoid) arthropathies, non-articular rheumatism, dystrophinopathy, Glycogenolysis, polymyositis, pyomyositis.

-Central pain or'thalamic pain'as defined by pain caused by lesion or dysfunction of the nervous system including but not limited to central post-stroke pain, multiple sclerosis, spinal cord injury, Parkinson's disease and epilepsy.

-Heart and vascular pain including but not limited to angina, myocardical infarction, mitral stenosis, pericarditis, Raynaud's phenomenon, scleredoma, scleredoma, skeletal muscle ischemia.

-Visceral pain, and gastrointestinal disorders. The viscera encompasses the organs of the abdominal cavity. These organs include the sex organs, spleen and part of the digestive system. Pain associated with the viscera can be divided into digestive visceral pain and non-digestive visceral pain. Commonly encountered gastrointestinal (GI) disorders include the functional bowel disorders (FBD) and the inflammatory bowel diseases (IBD). These GI disorders include a wide range of disease states that are currently only moderately controlled, including-for FBD, gastro-esophageal reflux, dyspepsia, the irritable bowel syndrome (IBS) and functional abdominal pain syndrome (FAPS), and-for IBD, Crohn's disease, ileitis, and ulcerative colitis, which all regularly produce visceral pain. Other types of visceral pain include the pain associated with dysmenorrhea, pelvic pain, cystitis and pancreatitis.

-Head pain including but not limited to migraine, migraine with aura, migraine without aura cluster headache, tension-type headache.

- Orofacial pain including but not limited to dental pain, temporomandibular myofascial pain.

Thus, as a yet further aspect of the present invention, there is provided the use of a compound of formula (1), or a pharmaceutically acceptable salt or solvate thereof, in the manufacture of a medicament for the treatment of pain, particularly neuropathic pain.

As an alternative aspect, there is provided a method for the treatment of pain, particularly neuropathic pain, comprising administration of a therapeutically effective amount of a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, to a mammal in need of said treatment.

General Synthesis All of the compounds of the formula (I) can be prepared by the procedures described in the general methods presented below or by the specific methods described in the Examples section and the Preparations section, or by routine modifications thereof. The present invention also encompasses any one or more of these processes for preparing the compounds of formula (I), in addition to any novel intermediates used therein.

In the following general methods, Cy, Rl, R2, R3, A, B, n, p, X, Y and Z are as previously defined for a compound of the formula (I), unless otherwise stated. The methods exemplify preparation of compounds of formula (I) where Y and X are in the trans configuration. It will be appreciated by those skilled in the art that compounds having a cis configuration may be prepared from the appropriate regiospecific starting materials or by separation of the alternative regioisomer from a mixture of cis and trans intermediates or final compounds.

According to process (A), a compound of the formula (1), where Y is-0-or- (CH2) qO- and q is 1-3, may be prepared by the reaction of a compound of the formula (IIa) or (IIb) with a compound of the formula (in) under standard Mitsunobu-type conditions, e. g. diisopropyl azodicarboxylate and Ph3P, in a suitable solvent such as tetrahydrofuran.

According to process (B), a compound of the formula (p, where Y is -(CH2)rOCH2- and r is 0-2, may be prepared by the reaction of a compound of the formula (IIc), with a compound of the formula (IV) where LG is a suitable leaving group, such as bromide, using a suitable base such as sodium hydride, in a suitable solvent such as dimethyl formamide.

According to process (C), a compound of the formula (I), where Z is N, Y is-(CH2) rO- and r is 0-3, may be prepared by the reaction of a compound of the formula (IIc), where r is 0-3, with a compound of the formula (IVa) where LG is a suitable leaving group, e. g. halogen, under suitable alkylating conditions, e. g. sodium hydride in a suitable solvent, such as DMF, at elevated temperature and in the presence of microwaves.

A compound of formula (II a-c) may be prepared by reaction of a compound of formula (Va) or (Vb) with a compound of formula (VI) where q is 1-3, P is a suitable hydroxy protecting group, e. g. benzyl, under standard acid/amine coupling conditions, e. g. using N-ethyl-N'- (3- dimethylaminopropyl) carbodiimide and 1-hydroxybenzotriazole (HOBT), in a suitable solvent such as dimethyl formamide, followed by removal of the P group under standard conditions, e. g. by hydrogenation.

A compound of formula (Va) or (Vb), where X is H, may be prepared from a compound of formula (VIIa) or (VIIb) where P is as defined above and Z'O is a suitable leaving group such as mesylate or tosylate, by treatment with a suitable azide, such as sodium azide, in a suitable solvent such as dimethyl formamide, at elevated temperature, followed by reduction of the azide to amino under standard conditions, such as hydrogenation. A compound of formula (Va) or (Vb), where A=B=C and X is OH, may be prepared from a compound of formula (VE) by treatment with a suitable cyanide, such as trimethylsilyl cyanide, with zinc iodide in toluene at reduced temperature, followed by reduction of the resulting cyano group with a suitable reducing agent, such as lithium aluminium hydride, and separation of the desired cis or trans-isomer.

Compounds of formula (VIIa) and (VIIb) may be prepared from a compound of formula (IXa) or (IXb) where R is a suitable ester group, e. g. methyl, by reduction with a suitable agent, e. g. lithium aluminium hydride, follwed by activation of the hydroxy group with Z'under suitable conditions.

According to process (D), a compound of the formula (I) may be prepared by the reaction of a compound of the formula (VI), with a compound of the formula (X) under standard acid/amine coupling conditions, such as N-ethyl-N'- (3- dimethylaminopropyl) carbodiimide and 1-hydroxybenzotriazole (HOBT) in a suitable solvent, such as dimethyl formamide.

A compound of formula (X), where A=B=C and X is OH, may be prepared from a compound of formula (XI) by reaction with a suitable cyanide compound, such as trimethylsilyl cyanide, with zinc iodide in a suitable solvent, such as toluene, at reduced temperature, followed by reduction with a suitable reducing agent, such as lithium aluminium hydride, and separation of the desired isomer.

A compound of formula (X), where X is H, may be prepared from a compound of formula (XII) where Z'O is defined above, by treatment with a suitable azide, such as sodium azide, in a suitable solvent such as dimethyl formamide, at elevated temperature, followed by reduction of the azide to amino under standard conditions, such as hydrogenation.

A compound of formula (XII) may be prepared from a compound of formula (XIIa) where R is a suitable ester group, e. g. methyl, by reduction with a suitable agent, e. g. lithium aluminium hydride, follwed by activation of the hydroxy group with Z'under suitable conditions.

A compound of formula (X) where Y is- (CH2) qO- and q is 1-3, may be prepared by reaction of a compound of formula (IV) with a compound of formula (Xnia) or (Xmb) where P'is a suitable N-protecting group, such as Boc, under Mitsunobu type conditions, as described above, followed by deprotection of the P'group under standard conditions.

A compound of formula (X) where Y is-(CH2) rOCH2-and r is 0-2, may be prepared by reaction of a compound of formula (IV) with a compound of formula (XHIc) where P'is defined above, under standard nucleophilic displacement conditions, as described above, followed by deprotection of the P'group under standard conditions.

Compounds of formula (XIIIa-c), where A=B=C and X is OH, may be prepared from a compound of formula (XIV) where q is 0-3, by reaction with a suitable cyanide compound, such as trimethylsilyl cyanide, with zinc iodide in a suitable solvent, such as toluene, at reduced temperature, followed by reduction with a suitable reducing agent, such as lithium aluminium hydride, and separation of the desired cis or trans-isomer.

A compound of formula (XIBa-c), where X is H, may be prepared from a compound of formula (Va) or (Vb) by selective protection of the amino group with a suitable protecting group P'followed by selective deprotection of the protecting group P.

A compound of formula (X), where X is H, may be prepared from a compound of formula (XI) by nitromethylation using nitromethane with a catalytic amount of ethylenediamine at elevated temperature followed by sequential reduction of the resulting nitro group and double bond under standard conditions.

A compound of formula (XI), where Y is-0-or- (CH2) qO- and q is 1-3, or Y is oxyethyleneoxy, may be prepared by reaction of a compound of formula (E) with a compound of formula (XVa) or (XVb), as appropriate under Mitsunobu type conditions, as described above, followed by deprotecton of the ketone group under standard conditions.

A compound of formula (XI), where Y is- (CH2) rOCH2- and r is 0-2 or Y is oxyethyleneoxy, may be prepared by reaction of a compound of formula (XVc) with a compound of formula (IV) or (Na), as appropriate under standard nucleophilic displacement conditions, as described above. A compound of formula (XI) where Y is a 1-4 membered alkylene may be prepared by reaction of a compound of formula (XVd) with a compound of formula (XVe) where Y'is a covalent bond or a 1-3 membered alkylene, under Wittig reaction conditions, followed by hydrogenation of the resulting double bond using a suitable metal catalyst, e. g.

PD (OH) 2 on carbon in a suitable solvent such as methanol, followed by deprotection of the keto group under suitable conditions.

A compound of formula (XI) where Y is- (CH2) qOCH2CH2- and q is 0-1, may be prepared by reaction of a compound of formula (XVa), where q is 0-1, with a compound of formula (XVI) using a suitable base, such as sodium hydride, in a suitable solvent, such as dimethyl formamide, followed by acetylation then reduction of the OH group under standard conditions, followed by deprotection of the ketone group.

A compound of formula (XII), where A=B=C and Y is-O (CH2) 2- may be prepared by reaction of compound of formula (XVII) with a compound of formula (XVM) where R is a suitable carboxylic acid ester protecting group, e. g. methyl, by treatment with p-toluenesulfonic acid in benzene followed by removal of one of the ether groups using, e. g. triethylsilane and trimethylsilyl triflate, followed by reduction of the ester under standard conditions, e. g. with lithium aluminium hydride, then activation of the hydroxy group with Z'under standard conditions.

A compound of formula (XII) where A=O and B=C, may be prepared from a compound of formula (XIX) by treatment with a suitable agent, such as p-toluenesulfonic acid, in a suitable solvent such as dichloromethane.

A compound of formula (VIIa) where A=O and B=C, may be prepared from a compound of formula (XX) where P is defined above, by treatment with a suitable agent, such as p-toluenesulfonic acid, in a suitable solvent such as dichloromethane, followed by deprotection of the protecting group.

According to a fifth process (E), a compound of formula (I), where A=B=C and Y represents a 1-4 membered alkylene, may be prepared by reaction of a compound of formula (XXI) : where Y'represents a covalent bond or a 1-3membered alkylene, by hydrogenation of the double bond using a suitable metal catalyst, e. g. Pd (OH) 2 on carbon in a suitable solvent such as methanol.

A compound of formula (XXI) may be prepared by reaction of a compound of formula (XXII) with a compound of formula (XVe) as described above under Wittig reaction conditions.

A compound of formula (XXII) may be prepared by reaction of a compound of formula (XXIII) with a compound of formula (VI) : under suitable acid amine coupling conditions as described above, followed by deprotection of the ketone group under suitable conditions.

Compounds of formulae (E), (IV), (VI), (VE), (IX), (XIIc), (XIV), (XV), (XVI), (XV), (XVE), (XIX), (XX) and (XXIII) are known in the art or may be prepared by well-known methods.

The use of protecting groups as described is well-known in the art. Suitable protecting groups for use in the afore-mentioned processes may be referenced in'Protecting Groups in Organic Synthesis', Greene and Wuts, 3rd Edition, John Wiley and Sons, Inc..

The various general methods described above may be useful for the introduction of the desired groups at any stage in the stepwise formation of the required compound, and it will be appreciated that these general methods can be combined in different ways in such multi- stage processes. The sequence of the reactions in multi-stage processes should of course be chosen so that the reaction conditions used do not affect groups in the molecule which are desired in the final product.

Compounds of the invention intended for pharmaceutical use may be administered as crystalline or amorphous products. They may be obtained, for example, as solid plugs, powders, or films by methods such as precipitation, crystallization, freeze drying, spray drying, or evaporative drying. Microwave or radio frequency drying may be used for this purpose.

They may be administered alone or in combination with one or more other compounds of the invention or in combination with one or more other drugs (or as any combination thereof). Generally, they will be administered as a formulation in association with one or more pharmaceutically acceptable excipients. The term"excipient"is used herein to describe any ingredient other than the compound (s) of the invention. The choice of excipient will to a large extent depend on factors such as the particular mode of administration, the effect of the excipient on solubility and stability, and the nature of the dosage form.

Pharmaceutical compositions suitable for the delivery of compounds of the present invention and methods for their preparation will be readily apparent to those skilled in the art. Such compositions and methods for their preparation may be found, for example, in 'Remington's Pharmaceutical Sciences', 19th Edition (Mack Publishing Company, 1995).

ORAL ADMINISTRATION The compounds of the invention may be administered orally. Oral administration may involve swallowing, so that the compound enters the gastrointestinal tract, or buccal or sublingual administration may be employed by which the compound enters the blood stream directly from the mouth.

Formulations suitable for oral administration include solid formulations such as tablets, capsules containing particulates, liquids, or powders, lozenges (including liquid-filled), chews, multi-and nano-particulates, gels, solid solution, liposome, films (including muco- adhesive), ovules, sprays and liquid formulations.

Liquid formulations include suspensions, solutions, syrups and elixirs. Such formulations may be employed as fillers in soft or hard capsules and typically comprise a carrier, for example, water, ethanol, polyethylene glycol, propylene glycol, methylcellulose, or a suitable oil, and one or more emulsifying agents and/or suspending agents. Liquid formulations may also be prepared by the reconstitution of a solid, for example, from a sachet.

The compounds of the invention may also be used in fast-dissolving, fast-disintegrating dosage forms such as those described in Expert Opinion in Therapeutic Patents, 11 (6), 981- 986 by Liang and Chen (2001).

For tablet dosage forms, depending on dose, the drug may make up from 1 wt% to 80 wt% of the dosage form, more typically from 5 wt% to 60 wt% of the dosage form. In addition to the drug, tablets generally contain a disintegrant. Examples of disintegrants include sodium starch glycolate, sodium carboxymethyl cellulose, calcium carboxymethyl cellulose, croscarmellose sodium, crospovidone, polyvinylpyrrolidone, methyl cellulose, microcrystalline cellulose, lower alkyl-substituted hydroxypropyl cellulose, starch, pregelatinised starch and sodium alginate. Generally, the disintegrant will comprise from 1 wt% to 25 wt%, preferably from 5 wt% to 20 wt% of the dosage form.

Binders are generally used to impart cohesive qualities to a tablet formulation. Suitable binders include microcrystalline cellulose, gelatin, sugars, polyethylene glycol, natural and synthetic gums, polyvinylpyrrolidone, pregelatinised starch, hydroxypropyl cellulose and hydroxypropyl methylcellulose. Tablets may also contain diluents, such as lactose (monohydrate, spray-dried monohydrate, anhydrous and the like), mannitol, xylitol, dextrose, sucrose, sorbitol, microcrystalline cellulose, starch and dibasic calcium phosphate dihydrate.

Tablets may also optionally comprise surface active agents, such as sodium lauryl sulfate and polysorbate 80, and glidants such as silicon dioxide and talc. When present, surface active agents may comprise from 0.2 wt% to 5 wt% of the tablet, and glidants may comprise from 0.2 wt% to 1 wt% of the tablet.

Tablets also generally contain lubricants such as magnesium stearate, calcium stearate, zinc stearate, sodium stearyl fumarate, and mixtures of magnesium stearate with sodium lauryl sulphate. Lubricants generally comprise from 0.25 wt% to 10 wt%, preferably from 0.5 wt% to 3 wt% of the tablet.

Other possible ingredients include anti-oxidants, colourants, flavouring agents, preservatives and taste-masking agents.

Exemplary tablets contain up to about 80% drug, from about 10 wt% to about 90 wt% binder, from about 0 wt% to about 85 wt% diluent, from about 2 wt% to about 10 wt% disintegrant, and from about 0.25 wt% to about 10 wt% lubricant.

Tablet blends may be compressed directly or by roller to form tablets. Tablet blends or portions of blends may alternatively be wet-, dry-, or melt-granulated, melt congealed, or extruded before tabletting. The final formulation may comprise one or more layers and may be coated or uncoated; it may even be encapsulated.

The formulation of tablets is discussed in"Pharmaceutical Dosage Forms: Tablets, Vol.

1", by H. Lieberman and L. Lachman, Marcel Dekker, N. Y. , N. Y. , 1980 (ISBN 0-8247- 6918-X).

Solid formulations for oral administration may be formulated to be immediate and/or modified release. Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release.

Suitable modified release formulations for the purposes of the invention are described in US Patent No. 6,106, 864. Details of other suitable release technologies such as high energy dispersions and osmotic and coated particles are to be found in Verma et al, Pharmaceutical Technology On-line, 25 (2), 1-14 (2001). The use of chewing gum to achieve controlled release is described in WO 00/35298.

PARENTERAL ADMINISTRATION The compounds of the invention may also be administered directly into the blood stream, into muscle, or into an internal organ. Suitable means for parenteral administration include intravenous, intraarterial, intraperitoneal, intrathecal, intraventricular, intraurethral, intrasternal, intracranial, intramuscular and subcutaneous. Suitable devices for parenteral administration include needle (including microneedle) injectors, needle-free injectors and infusion techniques.

Parenteral formulations are typically aqueous solutions which may contain excipients such as salts, carbohydrates and buffering agents (preferably to a pH of from 3 to 9), but, for some applications, they may be more suitably formulated as a sterile non-aqueous solution or as a dried form to be used in conjunction with a suitable vehicle such as sterile, pyrogen- free water.

The preparation of parenteral formulations under sterile conditions, for example, by lyophilisation, may readily be accomplished using standard pharmaceutical techniques well known to those skilled in the art.

The solubility of compounds of formula (I) used in the preparation of parenteral solutions may be increased by the use of appropriate formulation techniques, such as the incorporation of solubility-enhancing agents.

Formulations for parenteral administration may be formulated to be immediate and/or modified release. Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release. Thus compounds of the invention may be formulated as a solid, semi-solid, or thixotropic liquid for administration as an implanted depot providing modified release of the active compound. Examples of such formulations include drug-coated stents and PGLA microspheres.

TOPICAL ADMINISTRATION The compounds of the invention may also be administered topically to the skin or mucosa, that is, dermally or transdermally. Typical formulations for this purpose include gels, hydrogels, lotions, solutions, creams, ointments, dusting powders, dressings, foams, films, skin patches, wafers, implants, sponges, fibres, bandages and microemulsions.

Liposomes may also be used. Typical carriers include alcohol, water, mineral oil, liquid petrolatum, white petrolatum, glycerin, polyethylene glycol and propylene glycol. Penetration enhancers may be incorporated-see, for example, J Pharm Sci, 88 (10), 955- 958 by Finnin and Morgan (October 1999).

Other means of topical administration include delivery by electroporation, iontophoresis, phonophoresis, sonophoresis and microneedle or needle-free (e. g. Powderject, BiojectTM, etc.) injection.

Formulations for topical administration may be formulated to be immediate and/or modified release. Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release.

INHALED/INTRANASAL ADMINISTRATION The compounds of the invention can also be administered intranasally or by inhalation, typically in the form of a dry powder (either alone, as a mixture, for example, in a dry blend with lactose, or as a mixed component particle, for example, mixed with phospholipids, such as phosphatidylcholine) from a dry powder inhaler or as an aerosol spray from a pressurised container, pump, spray, atomiser (preferably an atomiser using electrohydrodynamics to produce a fine mist), or nebuliser, with or without the use of a suitable propellant, such as 1,1, 1,2-tetrafluoroethane or 1,1, 1,2, 3,3, 3-heptafluoropropane. For intranasal use, the powder may comprise a bioadhesive agent, for example, chitosan or cyclodextrin.

The pressurised container, pump, spray, atomizer, or nebuliser contains a solution or suspension of the compound (s) of the invention comprising, for example, ethanol, aqueous ethanol, or a suitable alternative agent for dispersing, solubilising, or extending release of the active, a propellant (s) as solvent and an optional surfactant, such as sorbitan trioleate, oleic acid, or an oligolactic acid.

Prior to use in a dry powder or suspension formulation, the drug product is micronised to a size suitable for delivery by inhalation (typically less than 5 microns). This may be achieved by any appropriate comminuting method, such as spiral jet milling, fluid bed jet milling, supercritical fluid processing to form nanoparticles, high pressure homogenisation, or spray drying.

Capsules (made, for example, from gelatin or HPMC), blisters and cartridges for use in an inhaler or insufflator may be formulated to contain a powder mix of the compound of the invention, a suitable powder base such as lactose or starch and a performance modifier such as l-leucine, mannitol, or magnesium stearate. The lactose may be anhydrous or in the form of the monohydrate, preferably the latter. Other suitable excipients include dextran, glucose, maltose, sorbitol, xylitol, fructose, sucrose and trehalose.

A suitable solution formulation for use in an atomiser using electrohydrodynamics to produce a fine mist may contain from lug to 20mg of the compound of the invention per actuation and the actuation volume may vary from lu. I to 1OOKLI. A typical formulation may comprise a compound of formula (I), propylene glycol, sterile water, ethanol and sodium chloride. Alternative solvents which may be used instead of propylene glycol include glycerol and polyethylene glycol.

Suitable flavours, such as menthol and levomenthol, or sweeteners, such as saccharin or saccharin sodium, may be added to those formulations of the invention intended for inhaled/intranasal administration.

Formulations for inhaled/intranasal administration may be formulated to be immediate and/or modified release using, for example, poly (DL-lactic-coglycolic acid (PGLA).

Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release.

In the case of dry powder inhalers and aerosols, the dosage unit is determined by means of a valve which delivers a metered amount. Units in accordance with the invention are typically arranged to administer a suitable metered dose or"puff'containing the compound of formula (I), which may be administered in a single dose or, more usually, as divided doses throughout the day.

RECTAL/INTRAVAGINAL ADMINISTRATION The compounds of the invention may be administered rectally or vaginally, for example, in the form of a suppository, pessary, or enema. Cocoa butter is a traditional suppository base, but various alternatives may be used as appropriate.

Formulations for rectal/vaginal administration may be formulated to be immediate and/or modified release. Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release.

OCULAR/AURAL ADMINISTRATION The compounds of the invention may also be administered directly to the eye or ear, typically in the form of drops of a micronised suspension or solution in isotonic, pH- adjusted, sterile saline. Other formulations suitable for ocular and aural administration include ointments, biodegradable (e. g. absorbable gel sponges, collagen) and non- biodegradable (e. g. silicone) implants, wafers, lenses and particulate or vesicular systems, such as niosomes or liposomes. A polymer such as crossed-linked polyacrylic acid, polyvinylalcohol, hyaluronic acid, a cellulosic polymer, for example, hydroxypropylmethylcellulose, hydroxyethylcellulose, or methyl cellulose, or a heteropolysaccharide polymer, for example, gelan gum, may be incorporated together with a preservative, such as benzalkonium chloride. Such formulations may also be delivered by iontophoresis.

Formulations for ocular/aural administration may be formulated to be immediate and/or modified release. Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted, or programmed release.

OTHER TECHNOLOGIES The compounds of the invention may be combined with soluble macromolecular entities, such as cyclodextrin and suitable derivatives thereof or polyethylene glycol-containing polymers, in order to improve their solubility, dissolution rate, taste-masking, bioavailability and/or stability for use in any of the aforementioned modes of administration.

Drug-cyclodextrin complexes, for example, are found to be generally useful for most dosage forms and administration routes. Both inclusion and non-inclusion complexes may be used. As an alternative to direct complexation with the drug, the cyclodextrin may be used as an auxiliary additive, i. e. as a carrier, diluent, or solubiliser. Most commonly used for these purposes are alpha-, beta-and gamma-cyclodextrins, examples of which may be found in International Patent Applications Nos. WO 91/11172, WO 94/02518 and WO 98/55148.

Thus, as a yet further or alternative aspect, the invention provides a pharmaceutical composition including a compound of formula (1), or a pharmaceutically acceptable salt or solvate thereof, together with a suitable excipient. The composition is useful in the treatment of a disease for which an NMDA NR2B antagonist is indicated, particularly pain, stroke, traumatic brain injury, Parkinson's disease, Alzheimer's disease, depression, anxiety and migraine.

KIT-OF-PARTS Inasmuch as it may desirable to administer a combination of active compounds, for example, for the purpose of treating a particular disease or condition, it is within the scope of the present invention that two or more pharmaceutical compositions, at least one of which contains a compound in accordance with the invention, may conveniently be combined in the form of a kit suitable for coadministration of the compositions.

Thus, the kit of the invention comprises two or more separate pharmaceutical compositions, at least one of which contains a compound of formula (1) in accordance with the invention, and means for separately retaining said compositions, such as a container, divided bottle, or divided foil packet. An example of such a kit is the familiar blister pack used for the packaging of tablets, capsules and the like.

The kit of the invention is particularly suitable for administering different dosage forms, for example, oral and parenteral, for administering the separate compositions at different dosage intervals, or for titrating the separate compositions against one another. To assist compliance, the kit typically comprises directions for administration and may be provided with a so-called memory aid.

DOSAGE For administration to human patients, the total daily dose of the compounds of the invention is typically in the range 0.1 mg to 1000 mg depending, of course, on the mode of administration. The quantity of active component in a unit dose preparation may be varied or adjusted from 0.1 mg to 1 g according to the particular application and the potency of the active components. In medical use the drug may be administered one to three times daily as, for example, capsules of 100 or 300 mg. In therapeutic use, the compounds utilized in the pharmaceutical method of this invention are administered at the initial dosage of about 0.01 mg to about 100 mg/kg daily. A daily dose range of about 0.01 mg to about 100 mg/kg is preferred.

These dosages are based on an average human subject having a weight of about 65kg to 70kg. The physician will readily be able to determine doses for subjects whose weight falls outside this range, such as infants and the elderly.

For the avoidance of doubt, references herein to"treatment"include references to curative, palliative and prophylactic treatment.

The biological activity and safety profile of the compounds of the formula (I) to may be measured using the assays described below.

NR2B Binding Assay The activity of the cycloalkylene amide compounds of the present invention, as NR2B antagonists, is determined by their ability to inhibit the binding of NR2B subunit at its receptor sites employing radioactive ligands.

The NR2B antagonist activity of the cycloalkylene amide compounds is evaluated by using the standard assay procedure described in, for example, J. Pharmacol., 331, ppll7- 126,1997. This method essentially involves determining the concentration of the individual compound required to reduce the amount of radiolabelled NR2B ligands by 50% at their receptor sites, thereby affording characteristic ICso values for each compound tested. More specifically, the assay is carried out as follows.

Membranes were prepared by homogenization of forebrain of male CD rats weighing between 170-190 g by using glass-Teflon homogenizer in 0.32 M sucrose at 4°C. The crude nuclear pellet was removed by centrifugation at 1000xg for 10 min, and the supernatant centrifuged at 17000xg for 25 min. The resulting pellet was resuspended in 5 mM Tris acetate pH 7.4 at 4°C for 10 min to lyse cellular particles and again centrifuged at 17000xg. The resulting pellet (P2 membrane) was washed twice in Tris acetate, resuspended at 5.5 mg protein/ml and stored at-20°C until use. All the manipulation was done on ice, and stock solution and equipment were kept on ice at all time.

For the saturation assay, receptor saturation was determined by incubating [3H]-1- [ S*, 2S*)-2-hydroxy-2- (4-hydroxyphenyl)-1-methylethyl]-4-phenylpiperidin-4-ol and 50 Fg protein of P2 membrane for 60 minutes at room temperature in a final 100 p1 of incubation buffer (50 mM Tris HCI, pH7.4). Total and non-specific bindings (in the presence of 10 RM of unlabeled l-[(lS*, 2S*)-2-hydroxy-2-(4-hydroxyphenyl)-1- methylethyl]-4-phenylpiperidin-4-ol) were determined in a range of [3 H]-I- [ (IS*, 2S*) -2- hydroxy-2- (4-hydroxyphenyl)-1-methylethyl]-4-phenylpiperidin-4-ol concentrations (0.625 nM to 60nM).

For the competition assay, test compounds were incubated in duplicate with 5 nM [3H]- 1- [ (1 S*, 2S*)-2-hydroxy-2- (4-hydroxyphenyl)-1-methylethyl]-4-phenylpiperidin-4-ol and 50 u. g protein of P2 membrane for 60 minutes at room temperature in a final 100 Ill of 50 mM Tris HCI buffer (pH7.4). Nonspecific binding was determined by 10 ItM of unlabeled 1- [ (lS*, 2S*)-2-hydroxy-2- (4-hydroxyphenyl)-1-methylethyl]-4-phenylpiperidin-4-ol (25 [tl).

The saturation derived Ko gained in saturation assay was used for all Ki calculations.

All incubations were terminated by rapid vacuum filtration over 0.2% polyethyleneimine soaked Whatman GF/B glass fibre filter paper using a SKATRON cell harvester followed by three washes with ice-cold filtration buffer (5 mM Tris HCI, pH 7.4.). Receptor-bound radioactivity was quantified by liquid scintillation counting using Packard LS counter.

Competition assays were performed by counting Wallac GF/B filters on Betaplate scintillation counter (Wallac).

The compound prepared in the working example 11 as described below was tested by this method, and showed a Ki value of 6.2 nM with respect to binding affinity for the NR2B receptor. In this test, the compounds of the present invention exhibited excellent binding activity for the NR2B receptor.

Human NR2B Cell Functional Assay HEK293 cells stably expressing human NRlb/2B receptor were used for cell functional assay. Cells were grown in 75-cm2 culture flasks, using Dulbecco's modified Eagle's medium (DMEM, high glucose) supplemented with 10% fetal bovine, 52 ttg/ml Zeocin, 530 g/ml Geneticin, 100 units/ml penicillin and 100 Rg/ml streptomycin. Cells were maintained in a humidified atmosphere in 5% C02 at 37°C, and 50-60% confluent cells were harvested by 0.05% trypsin containing 0.53 mM EDTA. The day before the experiment, expression of NRlb/2B receptor was induced by 5 FM ponasteron A in DMEM (40 ml) in the presence of 400 u. M ketamine to prevent excitotoxicity. The induction was performed for 19-24 hours, using 50-60% confluent cells.

Cells were washed with 10 ml of Ca2+-free Krebs-Ringer Hepes buffer (KRH) containing 400, uM ketamine, and the loading of 5 FM fura-2 acetoxymethyl ester was made for 2hrs at room temperature in the presence of 400 FM ketamine in Ca2+-free KRH (10 ml).

Subsequently, cells were collected in 50 ml tube by pipetting manipulation and centrifuged at 850 rpm for 2 min. Supernatant was removed, and cells were washed with 10 ml of Ca2+- free KRH buffer, followed by centrifugation again. This manipulation was repeated 4 times to remove ketamine, glutamate and glycine. Cells were re-suspended in Ca2+-free KRH buffer, and 50 Ill of cell suspension was addesud to each well of 96-well plates at a density of 100,000 cells/well, followed by adding test compounds dissolved in 50 ttl of Ca2+-free KRH. After pre-incubation for 30 min, agonists (final 100 FM glutamic acid and 10 OM glycine) dissolved in 25 y1 of KRH containing 9 mM Ca2+ (final 1.8 mM) were added. Fura-2 fluorescence (excitation wavelengths: 340 nm and 380 nm; emission wavelengths 510-520 nm) was monitored with a fluorescence imaging system, FDSS6000. The 0 fluorescence ratio F340/F380 (i. e. , the fluorescence ratio immediately post-agonist-the basal fluorescence ratio; calculated as AUC) was used for evaluation of drug effects on agonists-induced changes in intracellular Ca2+. The basal fluorescence ratio was determined in the presence of 10 FM MK-801.

Rat Haloperidol-Induced Catalepsy Assay Fasted male CD rats were used (7-8 weeks old). Test compound or vehicle was given subcutaneously then haloperidol 0.5 mg/kg s. c.. Sixty minutes after haloperidol-injection, the duration of catalepsy was quantified by placing the animals forepaws on an elevated bar and determining the latency to remove both forepaws from the bar. The cutoff latency was 60 seconds. The experimenter was blind to treatments during testing.

Human Dofetilide Binding Human HERG transfected HEK293S cells were prepared and grown in-house. The collected cells were suspended in 50 mM Tris-HCI (pH 7.4 at 4°C) and homogenized using a hand held Polytron PT 1200 disruptor set at full power for 20 sec on ice. The homogenates were centrifuged at 48,000 x g at 4 °C for 20 min. The pellets were then resuspended, homogenized, and centrifuged once more in the same manner. The final pellets were resuspended in an appropriate volume of 50 mM Tris-HCI, 10 mM KCI, 1 mM MgC12 (pH 7.4 at 4°C), homogenized, aliquoted and stored at-80°C until use. An aliquot of membrane fractions was used for protein concentration determination using BCA protein assay kit (PIERCE) and ARVOsx plate reader (Wallac).

Binding assays were conducted in a total volume of 200 Ill in 96-well plates. Twenty, ut of test compounds were incubated with 20 1 of [3H]-dofetilide (Amersham, final 5 nM) and 160 RI of membrane homogenate (25 u. g protein) for 60 minutes at room temperature.

Nonspecific binding was determined by 10 u. M dofetilide at the final concentration. Incubation was terminated by rapid vacuum filtration over 0. 5% presoaked GF/B Betaplate filter using Skatron cell harvester with 50 mM Tris-HCI, 10 mM KCI, 1 mM MgCl2, pH 7.4 at 4°C. The filters were dried, put into sample bags and filled with Betaplate Scint.

Radioactivity bound to filter was counted with Wallac Betaplate counter. kERG Assay HEK 293 cells which stably express the HERG potassium channel were used for electrophysiological study. The methodology for stable transfection of this channel in HEK cells can be found elsewhere (Z. Zhou et al., 1998, Biophysical journal, 74, pp230-241). Before the day of experimentation, the cells were harvested from culture flasks and plated onto glass coverslips in a standard MEM medium with 10% FCS. The plated cells were stored in an incubator at 37°C maintained in an atmosphere of 95% 02/5% C02. Cells were studied between 15-28hrs after harvest.

HERG currents were studied using standard patch clamp techniques in the whole-cell mode. During the experiment the cells were superfused with a standard external solution of the following composition (mM); NaCI, 130; KCI, 4; CaCI2, 2; MgCl2, 1; Glucose, 10; HEPES, 5; pH 7.4 with NaOH. Whole-cell recordings was made using a patch clamp amplifier and patch pipettes which have a resistance of 1-3MOhm when filled with the standard internal solution of the following composition (mM); KCI, 130; MgATP, 5; MgCI2, 1.0 ; HEPES, 10; EGTA 5, pH 7.2 with KOH. Only those cells with access resistances below 15MQ and seal resistances >1GQ was accepted for further experimentation. Series resistance compensation was applied up to a maximum of 80%. No leak subtraction was done. However, acceptable access resistance depended on the size of the recorded currents and the level of series resistance compensation that can safely be used. Following the achievement of whole cell configuration and sufficient for cell dialysis with pipette solution (>5min), a standard voltage protocol was applied to the cell to evoke membrane currents.

The voltage protocol is as follows. The membrane was depolarized from a holding potential of-80mV to +20mV for 1000ms. This was followed by a descending voltage ramp (rate 0. 5mV mec'') back to the holding potential. The voltage protocol was applied to a cell continuously throughout the experiment every 4 seconds (0.25Hz). The amplitude of the peak current elicited around-4. OmV during the ramp was measured. Once stable evoked current responses were obtained in the external solution, vehicle (0.5% DMSO in the standard external solution) was applied for 10-20 min by a peristalic pump. Provided there were minimal changes in the amplitude of the evoked current response in the vehicle control condition, the test compound of either 0.3, 1,3, 1041 was applied for a 10 min period. The 10 min period included the time which supplying solution was passing through the tube from solution reservoir to the recording chamber via the pump. Exposing time of cells to the compound solution was more than 5min after the drug concentration in the chamber well reached the attempting concentration. There reversibility. Finally, the cells was exposed to high dose of dofetilide (5uM), a specific IKr blocker, to evaluate the insensitive endogenous current.

All experiments were performed at room temperature (23 1°C). Evoked membrane currents were recorded on-line on a computer, filtered at 500-lKHz (Bessel-3dB) and sampled at 1-2KHz using the patch clamp amplifier and a specific data analyzing software. Peak current amplitude, which occurred at around-4OmV, was measured off line on the computer.

The arithmetic mean of the ten values of amplitude was calculated under control conditions and in the presence of drug. Percent decrease of IN in each experiment was obtained by the normalized current value using the following formula: IN = (1-ID/Ic) x100, where ID is the mean current value in the presence of drug and Ic is the mean current value under control conditions. Separate experiments were performed for each drug concentration or time-matched control, and arithmetic mean in each experiment is defined as the result of the study.

Mice PSL Method Surgery of partial sciatic nerve ligation (PSL) was made according to Seltzer et al. (Pain 43,1990, 205-218). Von Fray hair test was applied slowly to the plantar surface of the hind operated paw until the hairs bent. Each hair was tested 10 times in ascending order of force to different loci of the paw with one to two second intervals between each application. Once a withdrawal response was established, the paw was re-tested with the same hair. The lowest amount of force required to elicit a response was recorded as the paw-withdrawal threshold, measured in grams.

In vitro micronucleus assay In vitro micronucleus assay detects chemically induced micronucleus formation (chromosome breakage and/or whole chromosome loss) in vitro, by evaluating treated cultures of Chinese Hamster Ovary (CHO-WBL) cells. The growth medium is McCoy's 5A mediasupplemented with fetal bovine serum (FBS). Cells are incubated at approximately 37°C, 95% air/5% CO2 in a humidified chamber. Compound is dissolved in DMSO (dimethylsulfoxide). The final volume of compound in the medium is 1%. The maximum concentration of compound should be at or near a cytotoxic level. With non-toxic compound a maximum of 5mg/mL or the lowest precipitating concentration is used. Assay conditions include both direct assay and metabolic activation assay where the compound is tested in the presence of Aroclor 1254-induced rat liver S9 fraction.

Cultures are initiated by seeding approximately 1x104 exponentially growing CHO-WBL in McCoy's 5A medium into 8 well slide chamber. Twenty-four hours after the seeding, cells are treated with compounds. In direct assay, cells are treated with compound and Cytochalasin B for 24 hours. In metabolic activation assay, cells are treated with compound in the presence of rat liver S9 fraction for 3 hours, and then cells are incubated with a fresh medium including and Cytochalasin B for 21 hours. Approximately 24 hours from the initiation of treatment the cells are incubated in hypotonic buffer (75 mM KCI) for 5 min.

After the hypotonic treatment, the cells are fixed in the fixative solution (MeOH : acetic acid = 3 : 1 v/v) and stained with Acridine Orange. One hundred consecutive cells per concentration for the proportion of those with 1,2 or > 3 nuclei per cell and 1000 binucleated cells for the presence of micronuclei are analyzed (minimum 500 binucleated cells should be analyzed). A dose-dependent, two-fold or greater increase over negative control value is considered a positive response.

Serum Protein Binding Serum protein binding of NR2B topic compounds (1 uM) in humans and ddY mice were measured in method of equilibrium dialysis using 96-well plate type equipment. Spectra- Poto regenerated cellulose membranes (molecular weight cut-off 12,000-14, 000,12 mm x 120 mm) was soaked for over night in distilled water, then for 20 minutes in 30% ethanol, and finally for 15 minutes in dialysis buffer (0.10 M PBS: phosphate buffered saline, pH 7.4). Fresh humans and ddY mice serum (20 ml each) was prepared. The dialysis was assembled with being careful not to puncture or tear the membranes and added 150 u. I of serum to one side of each well and 150 ul of dialysis buffer to the other side of each well.

After 4 hours incubation at 37°C for 60 r. p. m, remove the serum and buffer samples and an aliquot of collected serum and buffer samples were mixed for buffer and serum at following rates: 1) 40 pLI serum samples were mixed with 120 RI buffer 2) 120 zI buffer samples were mixed with 40 gel serum Then, mixed samples were extracted with 600F1 acetonitrile containing (2R, 3R)-2- (diphenylmethyl)-N-(2-methoxybenzyl) quinuclidin-3-amine at 25 ng/ml (as HPLC-MS-MS internal standard) and measured in LC/MS/MS analysis.

Calculations: The fraction of substrate unbound, fu = 1 ( [plasma], q- [buffer] eq)/ ( [plasma] eq) where [plasma] eq and [buffer], q are the concentrations of substrate in plasma and buffer, respectively.

Aqueous Solubility Aqueous solubility in the mediums (a)- (c) was determined by method (1) or (2). (1) Vials containing approx. 1 mg of compound and 1 mL of each medium were agitated for 24 hours at room temperature. Insoluble materials were removed by centrifugation at 10,000 rpm for 10 minutes twice. The supernatants were assayed by HPLC. (2) Whatman Mini- UniPrep chambers (Clifton, NJ, USA) containing more than 0.5 mg of compound and 0.5 mL of each medium were shaken overnight (over 8 hours) at room temperature. All samples were filtered through a 0. 45 um PVDF membrane into a Whatman Mini-UniPrep plunger before analysis. The filtrates were assayed by HPLC.

<Mediums>: (a) Simulated gastric fluid with no enzyme (SGN) at pH 1.2 : Dissolve 2.0 g of NaCI in 7.0 mL of ION HCI and sufficient water to make 1000 mL.

(b) Phosphate buffered saline (PBS) at pH 6.5 : Dissolve 6.35 g of KH2PO4, 2. 84 g of Na2HP04 and 5.50 g of NaCI in sufficient water to make 1000 mL, adjusting the pH of this solution to 6.5.

(c) Water for injection (WFI).

Human VIA Binding Assay Cell paste of CHO cells expressing human Vla receptor was suspended in 3-fold volume of ice-cold wash buffer (50 mM Tris-HCI, 5 mM MgCl2, protease inhibitors, adjusted pH 7.4). The cells were homogenized and centrifuged at 25, 000g for 30 minutes at 4°C. The pellet was re-suspended by homogenization in freezing buffer (50 mM Tris-HCI, 5 mM MgCI2, 20% glycerol, adjusted pH 7.4). The membrane homogenate was stored at-80°C until use. All the manipulation was done on ice, and stock solution and equipment were kept on ice at all time.

For the saturation assay, receptor saturation was determined by incubating 8- Arg [phenylalanyl-3, 4, 5-3H]-vasopressin (3H-AVP) and 20 Fg protein of cell membrane for 60 minutes at 25°C in a final 250 p1 of incubation buffer (50 mM Tris-HCI, 5 mM MgCl2, 0. 05% BSA, adjusted pH 7.4). Total and non-specific bindings (in the presence of 1 iM of d (CH2) 5Tyr (Me) AVP [ß-mercapto-ß,ß-cyclopentamethylene propionyl, O-Me-Tyr2, Arg8]- vasopressin (ßMCPVP)) were determined in a range of 3H-AVP concentrations (0.05 nM to 100 nM).

For the competition assay, test compounds were incubated with 0.5 nM 3H-AVP and 20 llg protein of cell membrane for 60 minutes at 25°C in a final 250 1 of incubation buffer (50 mM Tris-HCI, 5 mM MgCl2, 0. 05% BSA, adjusted pH 7.4). Nonspecific binding was determined by 1 M of ßMCPVP. The saturation derived KD gained in saturation assay was used for all Ki calculations.

All incubations were terminated by filtration through Packard GF/C Unfilter plates pre- soaked in 0. 5% polyethyleneimine followed by three washes with ice-cold filtration buffer (50 mM Tris-HCI, 5 mM MgClz, adjusted pH 7.4). The plates were then placed back into the incubator at 50°C to dry. The bottom of the Unifilter plates were sealed using Packard plate seals and 50gui of Microscint 0 was added to each well. The plates were then sealed with Packard Topseal A, and receptor-bound radioactivity was counted by Packard Topcount NXT.

An NMDA NR2B antagonist of the present invention may be usefully combined with another pharmacologically active compound, or with two or more other pharmacologically active compounds, particularly in the treatment of pain. For example, an NMDA NR2B antagonist, particularly a compound of the formula (I), or a pharmaceutically acceptable salt or solvate thereof, as defined above, may be administered simultaneously, sequentially or separately in combination with one or more agents selected from: (i) opioid analgesics, e. g. morphine, heroin, hydromorphone, oxymorphone, levorphanol, levallorphan, methadone, meperidine, fentanyl, cocaine, codeine, dihydrocodeine, oxycodone, hydrocodone, propoxyphene, nalmefene, nalorphine, naloxone, naltrexone, buprenorphine, butorphanol, nalbuphine and pentazocine; (ii) nonsteroidal antiinflammatory drugs (NSAIDs), e. g. aspirin, diclofenac, diflusinal, etodolac, fenbufen, fenoprofen, flufenisal, flurbiprofen, ibuprofen, indomethacin, ketoprofen, ketorolac, meclofenamic acid, mefenamic acid, nabumetone, naproxen, oxaprozin, phenylbutazone, piroxicam, sulindac, tolmetin, zomepirac, and their pharmaceutically acceptable salts; (iii) barbiturate sedatives, e. g. amobarbital, aprobarbital, butabarbital, butabital, mephobarbital, metharbital, methohexital, pentobarbital, phenobartital, secobarbital, talbutal, theamylal, thiopental and their pharmaceutically acceptable salts; (iv) benzodiazepines having a sedative action, e. g. chlordiazepoxide, clorazepate, diazepam, flurazepam, lorazepam, oxazepam, temazepam, triazolam and their pharmaceutically acceptable salts, (v) Hl antagonists having a sedative action, e. g. diphenhydramine, pyrilamine, promethazine, chlorpheniramine, chlorcyclizine and their pharmaceutically acceptable salts; (vi) miscellaneous sedatives such as glutethimide, meprobamate, methaqualone, dichloralphenazone and their pharmaceutically acceptable salts; (vii) skeletal muscle relaxants, e. g. baclofen, carisoprodol, chlorzoxazone, cyclobenzaprine, methocarbamol, orphrenadine and their pharmaceutically acceptable salts, (viii) NMDA receptor antagonists, e. g. dextromethorphan ( (+)-3-hydroxy-N- methylmorphinan) and its metabolite dextrorphan ( (+)-3-hydroxy-N- methylmorphinan), ketamine, memantine, pyrroloquinoline quinone and cis-4- (phosphonomethyl) -2-piperidinecarboxylic acid and their pharmaceutically acceptable salts; (ix) alpha-adrenergic active compounds, e. g. doxazosin, tamsulosin, clonidine and 4- amino-6, 7-dimethoxy-2- (5-methanesulfonamido-1, 2,3, 4-tetrahydroisoquinol-2-yl)- 5- (2-pyridyl) quinazoline; (x) tricyclic antidepressants, e. g. desipramine, imipramine, amytriptiline and nortriptiline; (xi) anticonvulsants, e. g. carbamazepine and valproate; (xii) Tachykinin (NK) antagonists, particularly Nk-3, NK-2 and NK-1 e. g. antagonists, (aR, 9R)-7- [3, 5-bis (trifluoromethyl) benzyl]-8, 9,10, 11-tetrahydro-9- methyl-5- (4-methylphenyl)-7H- [1, 4] diazocino [2,1-g] [1,7] naphthridine-6-13-dione (TAK-637), 5-[[(2R, 3S)-2-[(1R)-1-[3, 5-bis (trifluoromethyl) phenyl] ethoxy-3- (4- fluorophenyl)-4-morpholinyl] methyl]-1, 2-dihydro-3H-1, 2,4-triazol-3-one (MK-869), lanepitant, dapitant and 3-[[2-methoxy-5-(trifluoromethoxy) phenyl] methylamino]-2- phenyl-piperidine (2S, 3S) (xiii) Muscarinic antagonists, e. g oxybutin, tolterodine, propiverine, tropsium chloride and darifenacin; (xiv) COX-2 inhibitors, e. g. celecoxib, rofecoxib and valdecoxib; (xv) Non-selective COX inhibitors (preferably with GI protection), e. g. nitroflurbiprofen (HCT-1026); (xvi) coal-tar analgesics, in particular, paracetamol; (xvii) neuroleptics, such as droperidol; (xviii) Vanilloid receptor agonists, e. g. resinferatoxin; (xix) Beta-adrenergic compounds such as propranolol; (xx) Local anaesthetics, such as mexiletine; (xxi) Corticosteriods, such as dexamethasone (xxii) serotonin. receptor agonists and antagonists; (xxiii) cholinergic (nicotinic) analgesics; (xxiv) miscellaneous agents such as Tramadol@ ; (xxv) PDEV inhibitors, such as sildenafil, vardenafil or taladafil; (xxvi) serotonin reuptake inhibitors, e. g. fluoxetine, paroxetine, citalopram and sertraline; (xxvii) mixed serotonin-noradrenaline reuptake inhibitors, e. g. milnacipran, venlafaxine and duloxetine; (xxviii) noradrenaline reuptake inhibitors, e. g. reboxetine; (xxix) atypical anti-psychotics, e. g. ziprasidone, olanzapine, clozapine, risperidone, sertindole, quetiapine, aripiprazole and amisulpride.

EXAMPLES The following Examples and Preparations illustrate the preparation of compounds of the formula (I).

'H Nuclear magnetic resonance (NMR) spectra were in all cases consistent with the proposed structures. Characteristic chemical shifts (8) are given in parts-per-million downfield from tetramethylsilane using conventional abbreviations for designation of major peaks: e. g. s, singlet; d, doublet; t, triplet; q, quartet; m, multiplet ; br, broad. The mass spectra (m/z) were recorded using either electrospray ionisation (ESI) or atmospheric pressure chemical ionisation (APCI). The following abbreviations have been used: CDC13, deuterochloroform ; D6-DMSO, deuterodimethylsulphoxide; CD30D, deuteromethanol; THF, tetrahydrofuran; MeOH, methanol; EtOH, ethanol; AcOEt, ethyl acetate; DMF, dimethyl formamide, EDCI, N-ethyl-N'- (3-dimethylaminopropyl) carbodiimide; HOBt, 1- hydroxybenzotriazole; DIAD, Diisopropyl azodicarboxylate; TBAF, tetrabutylammonium fluoride; TMSCN, trimethylsilylcyanide; PPh3, Triphenylphosphine; SEMCI, 2- (Trimethylsilyl) ethoxymethyl chloride; Pd-C, palladium carbon; mCPBA, m- Chloroperbenzoic acid. 'Ammonia'refers to a concentrated solution of ammonia in water possessing a specific gravity of 0. 88. Where thin layer chromatography (TLC) has been used it refers to silica gel TLC using silica gel 60 F254 plates, Rf is the distance travelled by a compound divided by the distance travelled by the solvent front on a TLC plate.

Example 1 4-Hydroxy-N-(fcis-4- (phenoxvmethyl) cyclohexyllmethyl benzamide DIAD (0. 89 mL, 4.5 mmol) was added dropwise to a mixture of 4-(benzyloxy)-N-{[cis- 4-(hydroxymethyl) cyclohexyl] methyl} benzamide (1.1 g, 3. 0 mmol), phenol (0.42 g, 4.5 mmol) and triphenylphosphine (1.2 g, 4.5 mmol) in THF (10 mL) at 0 °C. The mixture was stirred at room temperature for 8 hours and quenched with water and 2 N aq. NaOH. The whole was extracted with CH2CI2. The extract was dried over MgS04 and concentrated in vacuum. The residue was purified by silica gel column chlomatography (hexane: AcOEt = 3: 1) to afford 4-(benzyloxy)-N-{[cis-4-(phenoxymethyl) cyclohexyl] methyl}benzamide. A mixture of 4-(benzyloxy)-N-{[cis-4-(phenoxymethyl) cycloheXylgmethyl} benzamide and 10% Pd-C (0.20 g) was hydrogenated at 1 atm for 3 hours. The mixture was filtered through a pad of celite and the filtrate was evaporated. The residue was purified by silica gel column chromatography (hexane: AcOEt = 2: 1) to give the titled compound (0. 68 g).

'H NMR (CD3) § : 7.66 (d, J= 8.4 Hz, 2H), 7.31-7. 24 (m, 2 H), 6.96-6. 84 (m, 5H), 6.10- 6.00 (m, 1H), 3.86 (d, J = 6.9 Hz, 2H), 3.42 (t, J = 6.4 Hz, 2H), 2.10-1. 40 (m, 10H) ppm.

(OH was not observed.) MS (ESI): 340.18 (M+H) +, 338.15 (M-H)- Example 1A 4-Hvdroxy-N fcis-4-(phenoxymethyl) cyclohexyllmethyllbenzamide sodium salt To a solution of 4-hydroxy-N-{[cis-4-(phenoxymethyl) cyclohexyl] methyl} benzamide (0. 68 g, 2.0 mmol) in EtOH (20 mL), 2 N aq. NaOH (0.95 mL) was added and the mixture was concentrated in vacuum. The solid was washed with CH2C12 and filtered to give the titled compound (0.53 g) as a white solid.

'H NMR (DMSO-d6) 8 : 7.44 (t, J = 5.7 Hz, 1H), 7.37-7. 23 (m, 4 H), 6.96-6. 88 (m, 3H), 5.97 (d, J = 8.8 Hz, 2H), 3. 86 (d, J = 7.0 Hz, 2H), 3.13 (t, J = 6.6 Hz, 2H), 1.96-1. 30 (m, 10H) ppm.

MS (ESI) : 340.24 (M+H) +, 338.19 (M-H)- IR (KBr) vma, : 3350,1599, 1547,1497, 1296,1246, 1175, 1035 cm-1 Example 2 4-Hydroxy-N-({cis-4-[(4-methoxyphenoxy)methyl]cyclohexyl}met hyl)benzamide A mixture of 4- (benzyloxy)-N ( (cis-4- [ (4- methoxyphenoxy) methyl] cyclohexyl} methyl) benzamide (70 mg, 0.15 mmol) and 10% Pd-C (15 mg) in MeOH (10 mL) was hydrogenated at 1 atm for 2 hours. The mixture was filtered through a pad of celite and the filtrate was evaporated. The residue was purified by silica gel column chromatography (hexane-AcOEt 1: 1) and crystallization from CH2CI2-hexane to give the titled compound (41 mg).

'H NMR (CDC13) # : 7.65 (d, J = 8. 6 Hz, 2H), 6.90-6. 81 (m, 6H), 6.62 (br, 1H), 6.13-6. 05 (m, 1H), 3.81 (d, J = 6.8 Hz, 2H), 3.77 (s, 3H), 3.46-3. 39 (m, 2H), 2.05-1. 40 (m, 10H) ppm.

MS (ESI) : 370.7 (M+H) +, 367.9 (M-H)- IR (KBr) Vmax : 3119,2926, 1601,1501, 1450,1281, 1227, 1177 cm~ Example 3 N-f fcis-4- (Benzvloxy) cyclohexvllmethvl-4-hvdroxbenzamide A mixture of 4-hydroxybenzoic acid (0.17 g, 1.2 mmol), [cis-4- (benzyloxy) cyclohexyl] methylamine (0. 25 g, 1.2 mmol) HOBt-H20 (0.21 g, 1.4 mmol), and EDCI (0.27 g, 1.4 mmol) in DMF (12 mL) was stirred at room temperature for 16 hours. 2 N aq. NaOH (10 mL) was added and the mixture was stirred for 1 hour. The mixture was neutralized with 2 N aq. HCl (10 mL) and extracted with AcOEt. The extract was washed with sat. aq. NaHC03 and water. The organic layer was dried over MgS04 and concentrated in vacuo. The residue was purified by silica gel column chromatography (hexane: AcOEt = 1: 1).

'H NMR (CDCI3) 8 : 7.63 (d, J = 8.6 Hz, 2H), 7.36-7. 24 (m, 5H), 7.09 (s, 1H), 6. 85 (d, J = 8. 6 Hz, 2H), 6.23-6. 15 (m, 1H), 4.50 (s, 2H), 3.68-3. 62 (m, 1H), 3.33 (t, J = 6.2 Hz, 2H), 2.00-1. 40 (m, 9H) ppm.

MS (ESI) : 340.21 (M+H) +, 338. 18 (M-H)- IR (KBr) Vmax : 3227, 2926,1612, 1508,1439, 1277,1175, 1094,1045 cm~ Example 4 N- (f cis-4-f (4-Chlorobenzyl) oxylcyclohexyl} methvl)-4-hydroxvbenzamide NaH (60%, 9.6 mg, 0.24 mmol) was added to a solution of N-[(cis-4- hydroxycyclohexyl) methyl]-4- (methoxymethoxy) benzamide (60 mg, 0.20 mmol) in DMF (1.0 mL) and the mixture was stirred at room temperature for 30 min. To the mixture, 4- chlorobenzylbromide (49 mg, 0.24 mmol) was added and the mixture was stirred at room temperature for 2 hours. To the mixture, 10% HCl-MeOH (2.0 mL) was added at room temperature and the mixture was stirred at 50 °C for 30 min. The mixture was diluted with AcOEt and washed with sat. aq. NaHC03 and water. The organic layer was dried over MgS04 and was evaporated. The residue was purified by prep. TLC (hexane: AcOEt = 1: 2) to give the titled compound (2.0 mg). 'H NMR (CDCl3) 8 : 7.65 (d, J = 8.7 Hz, 2H), 7. 32-7. 26 (m, 4H), 6.85 (d, J = 8. 6 Hz, 2H), 6.20-6. 10 (m, 1H), 4.45 (s, 2H), 3.67-3. 60 (m, 1H), 3.37-3. 30 (m, 2H), 2. 05-1. 40 (m, 9H) ppm. (OH was not observed.) MS (ESI) : 374.0 (M+H) +, 371.9 (M-H)- Example 5 N- ({cis-4-[(3-Chlorobenzyl)oxy]cyclohexyl}methyl)-4-hydroxyben zamide This compound was prepared with 3-chlorobenzylbromide by a procedure similar to that in Example 4.

'H NMR (CDCl3) 8 : 7.64 (d, J = 8.6 Hz, 2H), 7.36-7. 20 (m, 4H), 6.85 (d, J = 8.7 Hz, 2H), 6.20-6. 10 (m, 1H), 4.46 (s, 2H), 3.67-3. 60 (m, 1H), 3.38-3. 30 (m, 2H), 2. 05-1. 40 (m, 9H) ppm. (-OH was not observed.) MS (ESI) : 374.0 (M+H) +, 371.9 (M-H)- Example 6 4-Hydroxy-N-f [cis-4-(4-methoxyphenoxy) cyclohexvll methyl} benzamide A mixture of 4-(methoxymethoxy)-N-{[cis-4-(4- methoxyphenoxy) cyclohexyl] methyl} benzamide (11 mg, 0.027 mmol) and 10% HCI- MeOH (1.0 mL) was stirred at 50 °C for 30 min. The mixture was evaporated. The residue was purified by silica gel column chromatography (hexane: AcOEt = 1: 2) to give the titled compound (10 mg).

'H NMR (CDC13) 6 : 7.65 (d, J = 8.6 Hz, 2H), 7.19 (br, 1H), 6.90-6. 79 (m, 6H), 6.28-6. 18 (m, 1H), 4.45-4. 38 (m, 1H), 3.77 (s, 3H), 3.36 (t, J = 6.4 Hz, 2H), 2.10-1. 45 (m, 9H) ppm.

MS (ESI) : 356.24 (M+H) +, 354.21 (M-H)- IR (KBr) VmaX 3327,2930, 1609, 1506, 1443,1281, 1229,1177, 1038 cm~ Example 7 N-f [cis-4- (4-Chlorophenoxv) cyclohexvllmethvl)-4-hydroxybenzamide This compound was prepared with N-I [cis-4- (4-chlorophenoxy) cyclohexyl] methyl}-4- (methoxymethoxy) benzamide by a procedure similar to that in Example 6.

'H NMR (CDC13) 6 : 7.69 (s, 1H), 7.62 (d, J = 8.7 Hz, 2H), 7.20 (d, J = 8.9 Hz, 2H), 6.90- 6.78 (m, 4 H), 6.32-6. 22 (m, 1H), 4.52-4. 45 (m, 1H), 3.35 (t, J = 6.3 Hz, 2H), 2.10-1. 96 (m, 2H), 1.78-1. 40 (m, 7H) ppm.

MS (ESI) : 360.19 (M+H) +, 358.14 (M-H)- IR (KBr) vmax : 3358, 2928, 1609, 1508, 1489,1443, 1281, 1242,1173 cm-1 Example 8 4-Hvdroxy-N {[trans-1-hydroxy-4-(phenoxymethyl)cyclohexyl]methyl}benzami de A mixture of 1- (aminomethyl)-4- (phenoxymethyl) cyclohexanol hydrochloride (1.1 g, 4.0 mmol), 4-hydroxybenzoic acid (0.79 g, 4.4 mmol), HOBt-H2O (0.12 g, 0.8 mmol), Et3N (1.1 mL, 8.0 mmol), and EDCI (0.92 g, 4.8 mmol) in DMF (40 mL) was stirred at room temperature for 16 hours. 2 N aq. NaOH (15 mL) and MeOH (lOmL) were added and the mixture was stirred at room temperature for 4 hours. The mixture was neutrized with 2 N aq.

HCl (15 mL) and extracted with AcOEt. The extract was washed with sat. aq. NaHC03 and water, dried over MgSO4, and evaporated. The residue was purified by silica gel column chlomatography (CH2CI2 : MeOH = 25: 1) to give the titled compound (0.43 g).

'H NMR (DMSO-d6) 8 : 7.92 (t, J = 5.5 Hz, 1H), 7.73 (d, J = 8. 8 Hz, 2H), 7.32-7. 22 (m, 2 H), 6.96-6. 76 (m, 5H), 4.73 (br, 1H), 3.82 (d, J= 6.2 Hz, 2H), 3. 40-3. 34 (m, 2H), 1.84-1. 20 (m, 9H) ppm. (-OH was not obserbed.) MS (ESI) : 356. 17 (M+H) +, 354.13 (M-H)- IR (KBr) v,,,. : 3190,2931, 2864, 1608, 1541,1512, 1247,1174, 1224,1043 cm~ m. p. 189. 5°C Example 9 N-({trans-4-[(4-Fluorophenoxy)methyl]-1-hydroxycyclohexyl}me thyl)-4- hydroxybenzamide A mixture of N { [trans-1-hydroxy-4- (hydroxymethyl) cyclohexyl] methyl}-4- (methoxymethoxy) benzamide (97 mg, 0.30 mmol), 4-fluorophenol (50 mg, 0.45 moml) and cyanomethylenetributylphosphorane (0.12 g, 0.45 mmol) in toluene (1.5 mL) was stirred at 90 °C for 1 hour. After cooling to room temperature, the mixture was purified by silica gel column chromatography (hexane: AcOEt = 2 : 1) to give N ( {trans-4- [ (4- fluorophenoxy) methyl]-1-hydroxycyclohexyl} methyl)-4-(methoxymethoxy) benzamide. N- ({trans-4-[(4-Fluorophenoxy) methyl]-1-hydroxycyclohexyl} methyl)-4- (methoxymethoxy) benzamide was dissolved with 10% HCI-MeOH (2.0 mL) and the mixture was stirred at 50°C for 30 min. After evaporation, the residue was purified by silica gel column chromatography (CH2CI2 : MeOH = 30 : 1) to the titled compound (57 mg) as a white solid.

'H NMR (DMSO-d6) 8 : 10.00 (br, 1H), 7.95-7. 88 (m, 1H), 7.73 (d, J = 8.6 Hz, 2H), 7.13- 7.05 (m, 2 H), 6.96-6. 90 (m, 2H), 6.79 (d, J = 8.6 Hz, 2H), 4.71 (br, 1H), 3.80 (d, J = 6.0 Hz, 2H), 3.37 (d, J = 6.0 Hz, 2H), 1.82-1. 60 (m, 5H), 1.35-1. 14 (m, 4H) ppm.

MS (ESI) : 374.21 (M+H) +, 372.13 (M-H)- IR (KBr) Vma, : 3379,2937, 1630,1611, 1555, 1508, 1248, 1207 cm-1 m. p. 176. 4°C Example 10 N-( trans-4-f (3-Fluorophenoxy) methyll-1-hydroxycyclohexyl methyl)-4- hydroxybenzamide This compound was prepared with 3-fluorophenol by a procedure similar to that in Example 9.

'H NMR (DMSO-d6) b : 9.97 (br, 1H), 7.96-7. 88 (m, 1H), 7.73 (d, J = 8.6 Hz, 2H), 7.34- 7.24 (m, 1H), 6.84-6. 70 (m, 5H), 4.71 (br, 1H), 3.84 (d, J = 6.1 Hz, 2H), 3.39-3. 34 (m, 2H), 1.84-1. 62 (m, 5H), 1.40-1. 24 (m, 4H) ppm.

MS (ESI) : 374. 18 (M+H) +, 372.13 (M-H)- IR (KBr) vm,, : 3248,2937, 1630, 1611, 1593,1508, 1277,1136, 1119 cm-1 m. p. 186. 0 °C Example 11 N-({trans-4-[(2-Fluorophenoxy)methyl]-1-hydroxycyclohexyl}me thyl)-4- hydroxybenzamide This compound was prepared with 2-fluorophenol by a procedure similar to that in Example 9.

'H NMR (DMSO-d6) 6 : 10.00 (br, 1H), 7.96-7. 89 (m, 1H), 7.73 (d, J= 8.6 Hz, 2H), 7.24- 7.07 (m, 3H), 6.96-6. 87 (m, 1H). 6.79 (d, J = 8.6 Hz, 2H), 4.72 (br, 1H), 3.90 (d, J = 6.4 Hz, 2H), 3.40-3. 34 (m, 2H), 1.90-1. 62 (m, 5H), 1. 40-1. 20 (m, 4H) ppm.

MS (ESI): 374.22 (M+H) +, 372.16 (M-H)- IR (KBr) vmax: 3252, 2937, 1630, 1611, 1508, 1277,1256, 1109 cm-1 m. p. 185. 0 °C Example 12 N- (f trans-4-r (2, 6-Difluorophenoxy) methyll-l-hydroxvcyclohexyllmethyl)-4- hydroxybenzamide This compound was prepared with 2,6-difluorophenol by a procedure similar to that in Example 9.

'H NMR (DMSO-d6) S : 10.00 (br, 1H), 7.97-7. 89 (m, 1H), 7.73 (d, J = 8.8 Hz, 2H), 7. 18- 7.08 (m, 3H), 6.79 (d, J = 8.6 Hz, 2H), 4. 73 (br, 1H), 3.95 (d, J = 6.0 Hz, 2H), 3. 38-3. 34 (m, 2H), 1.82-1. 62 (m, 5H), 1.40-1. 25 (m, 4H) ppm.

MS (ESI) : 392.18 (M+H) +, 390.14 (M-H)- IR (KBr) Vma, : 3150, 2950, 1638, 1508, 1238 cm~ m. p. 153. 7°C Example 13 N-(({trans-4-f (3, 5-Difluorophenoxy) methyll-1-hydroxycyclohexyl} methyl)-4- hydroxybenzamide This compound was prepared with 3, 5-difluorophenol by a procedure similar to that in Example 9.

'H NMR (DMSO-d6) 8 : 10.00 (br, IH), 7.95-7. 88 (m, 1H), 7.73 (d, J = 8.6 Hz, 2H), 6.82- 6.68 (m, SH), 4.71 (br, 1H), 3.86 (d, J= 6.4 Hz, 2H), 3.40-3. 34 (m, 2H), 1.85-1. 60 (m, 5H), 1. 40-1. 20 (m, 4H) ppm.

MS (ESI) : 392.15 (M+H)+, 390.09 (M-H)- IR (KBr) Vmax : 3256,2941, 1624,1508, 1466,1285, 1153,1115 cm m. p. 102. 4 °C Example 14 N-({trans-4-[(2-Chlorophenoxy)methyl]-1-hydroxycyclohexyl}me thyl)-4- hydroxybenzamide This compound was prepared with 2-chlorophenol by a procedure similar to that in Example 9.

'H NMR (DMSO-d6) S : 9.97 (br, 1H), 7.96-7. 88 (m, 1H), 7.74 (d, 7= 8. 6 Hz, 2H), 7.41 (dd, J = 1.7, 8.9 Hz, 1H), 7.32-7. 25 (m, 1H), 7. 15 (dd, J = 1.5, 8.2 Hz, 1H), 6.97-6. 90 (m, 1H), 6.80 (d, J = 8.6 Hz, 2H), 4.71 (br, 1H), 3.91 (d, J = 6.4 Hz, 2H), 3.40-3. 35 (m, 2H), 1.90- 1. 60 (m, 5H), 1.40-1. 25 (m, 4H) ppm.

MS (ESI): 390.17, 392.17 (M+H) +, 388. 09,389. 98 (M-H)- IR (KBr) Vmax : 3296,2934, 1634,1508, 1468,1281, 1252 cm~ m. p. 159. 0 °C Example 15 N-({trans-4-[(3-Chlorophenoxy)methyl]-1-hydroxycyclohexyl}me thyl)-4- hydroxybenzamide This compound was prepared with 3-chlorophenol by a procedure similar to that in Example 9.

'H NMR (DMSO-d6) 8 : 10.00 (br, 1H), 7.96-7. 88 (m, 1H), 7.73 (d, J = 8. 8 Hz, 2H), 7.28 (t, J = 8.1 Hz, 1H), 7.03-6. 88 (m, 3H), 6.80 (d, J = 8. 6 Hz, 2H), 4.71 (br, 1H), 3.85 (d, J = 6. 1 Hz, 2H), 3.40-3. 35 (m, 2H), 1.84-1. 60 (m, SH), 1.40-1. 25 (m, 4H) ppm.

MS (ESI) : 390. 15 (M+H)+, 388.06 (M-H)- IR (KBr) v : 3179,2928, 1636,1593, 1512, 1458, 1286,1236, 1042 cm m. p. 164. 9 °C Example 16 N-({trans-4-[(4-Clorophenoxy)methyl]-1-hydroxycyclohexyl}met hyl)-4- hydroxybenzamide This compound was prepared with 4-chlorophenol by a procedure similar to that in Example 9.

'H NMR (DMSO-d6) 8 : 9.99 (br, 1H), 7.96-7. 89 (m, 1H), 7.73 (d, J = 8.6 Hz, 2H), 7.31 (d, J = 9.0 Hz, 2H), 6.96 (d, J = 9.0 Hz, 2H), 6.79 (d, J = 8. 6 Hz, 2H), 4.72 (br, 1H), 3. 82 (d, J = 6.4 Hz, 2H), 3.40-3. 35 (m, 2H), 1. 82-1. 60 (m, 5H), 1.40-1. 20 (m, 4H) ppm.

MS (ESI) : 390.13 (M+H) +, 388. 08 (M-H)- IR (KBr) vma, : 3198,2941, 1631, 1508, 1491,1279, 1244, 1121 cm'' m. p. 208. 2 °C Example 17 4-Hydroxy-N- (f trans-I-hydroxy-4-r (2- methylphenoxy) methyl]cyclohexyl}methyl)benzamide This compound was prepared with 2-methylphenol by a procedure similar to that in Example 9.

IH NMR (DMSO-d6) 6 : 9.99 (br, 1H), 7.96-7. 88 (m, 1H), 7.73 (d, J = 8. 8 Hz, 2H), 7.16- 7.09 (m, 2H), 6.92-6. 75 (m, 4H), 4.72 (br, 1H), 3. 82 (d, J = 6. 0 Hz, 2H), 3. 38 (d, J = 5. 9 Hz, 2H), 2.16 (s, 3H), 1.86-1. 60 (m, 5H), 1.42-1. 25 (m, 4H) ppm.

MS (ESI) : 370.18 (M+H)+, 368.12 (M-H)- IR (KBr) Vma, : 3231, 2936,1628, 1533,1497, 1281, 1244, 1121 cm~ m. p. 189. 1 °C Example 18 4-Hydroxy-N- trans-l-hydroxy-4-[(3- methylphenoxy)methyl]cyclohexyl}methyl)benzamide This compound was prepared with 3-methylphenol by a procedure similar to that in Example 9.

IH NMR (DMSO-d6) 8 : 9.99 (br, 1H), 7.97-7. 89 (m, 1H), 7.73 (d, J= 8.6 Hz, 2H), 7.14 (t, J = 7.9, 1H), 6.82-6. 68 (m, 5H), 4.72 (br, 1H), 3.79 (d, J = 6.0 Hz, 2H), 3.40-3. 35 (m, 2H), 2.26 (s, 3H), 1. 82-1. 62 (m, 5H), 1.40-1. 20 (m, 4H) ppm.

MS (ESI) : 370.21 (M+H) +, 368.13 (M-H)- IR (KBr) via, : 3227,2934, 1636, 1611, 1508, 1281, 1157 cm-' m. p. 201. 40 °C Example 19 4-Hydroxy-N-({trans-1-hydroxy-4-[ (4- methylphenoxy)methyl]cyclohexyl}methyl)benzamide This compound was prepared with 4-methylphenol by a procedure similar to that in Example 9.

'H NMR (DMSO-d6) 8 : 10.00 (br, 1H), 7.96-7. 89 (m, 1H), 7.73 (d, J = 8.6 Hz, 2H), 7.06 (d, J = 8. 3 Hz, 2H), 6.83-6. 76 (m, 4H), 4.72 (br, 1H), 3.77 (d, J = 6.2 Hz, 2H), 3.40-3. 35 (m, 2H), 2.22 (s, 3H), 1.82-1. 60 (m, 5H), 1.40-1. 20 (m, 4H) ppm.

MS (ESI) : 370.21 (M+H) +, 368.16 (M-H)- IR (KBr) vmax : 3246,2941, 1632,1508, 1279,1246, 1119cm~ m. p. 203. 1 °C Example 20 4-Hvdroxy-N traz2s-1-hydroxv-4- [(3- methoxyphenoxy) methyllcyclohexvl} methyl) benzamide Diisopropyl azodicarboxylate (0.30 mL, 1.5 mmol) was added dropwise to a mixture of N-{[trans-1-hydroxy-4-(hydroxymethyl) cyclohexyl] methyl}-4- (methoxymethoxy) benzamide (0.32 g, 1.0 mmol), 3-methoxyphenol (0.19 g, 1.5 mmol) and triphenylphosphine (0.39 g, 1.5 mmol) in THF at 0 °C and the mixture was stirred at room temperature for 2 hours. The mixture was treated with 2N aq. NaOH and was extracted with CH2C12. The extract was washed with sat. aq. NaCI, dried over MgS04, and then concentrated in vacuo. The residue was purified by silica gel column chromatography (hexane: AcOEt = 1 : 1) to give N-({trans-1-hydroxy-4-[(3- methoxyphenoxy) methyl] cyclohexyl} methyl)-4- (methoxymethoxy) benzamide. N- ( {trans-1- hydroxy-4- [ (3-methoxyphenoxy) methyl] cyclohexyl} methyl)-4- (methoxymethoxy) benzamide was dissolved in 10% HCl-MeOH and the mixture was stirred at 50 °C for 30 min. The mixture was evaporated and the residue was purified by silica gel column chromatography (CH2C12 : MeOH = 15: 1), followed by prep. TLC (CH2CI2 : MeOH = 8 : 1) to give the titled compound (0.21 g) as a white solid.

'H NMR (DMSO-d6) 8 : 9.96 (br, 1H), 7.90 (t, J = 5.6, 1H), 7.73 (d, J = 8.9 Hz, 2H), 7.20- 7.11 (m, 1H), 6.80 (d, J = 8. 6 Hz, 2H), 6.54-6. 46 (m, 3H), 4.70 (br, 1H), 3. 80 (d, J = 6.1 Hz, 2H), 3.72 (s, 3H), 3.37 (d, J = 5.8 Hz, 2H), 1. 85-1. 60 (m, 5H), 1.42-1. 20 (m, 4H) ppm.

MS (ESI): 386.14 (M+H) +, 384.13 (M-H)- IR (KBr) Vmax : 3229,2941, 1636,1587, 1508, 1279,1155 cm m. p. 190. 3°C Example 21 N- (trans-4-f (Benzvloxy) methyll-1-hydroxvcvclohexyl} methyl)-4-hvdroxybenzamide A mixture of N-({trans-4-[(benzyloxy) methyl]-1-hydroxycyclohexyl} methyl)-4- (methoxymethoxy) benzamide (0.37 g, 1.0 mmol) and 10% HCI-MeOH (10 mL) was stirred at 50 °C for 1 hour. After evaporation, the residue was purified by silica gel column chromatography (hexane: AcOEt = 2: 3) to give the titled compound (0.21 g).

'H NMR (DMSO-d6) 6 : 9.99 (br, 1H), 7.91 (t, J = 5.7, 1H), 7.73 (d, J = 8.6 Hz, 2H), 7.38- 7.24 (m, 5H), 6. 80 (d, J = 8.6 Hz, 2H), 4.71 (br, 1H), 4.45 (s, 2H), 3.36-3. 24 (m, 4H), 1.70- 1.54 (m, 5H), 1.36-1. 08 (m, 4H) ppm.

MS (ESI) : 368.11 (M-H)- IR (KBr) Vmax : 3242,2941, 1609, 1508, 1275,1115 cm~ m. p. 165. 7°C Example 22 N-[(trans-4-{[(2-Fluorobenzyl)oxy]methyl}-1-hydroxycyclohexy l)methyl]-4- hydroxybenzamide NaH (60%, 20 mg, 0.5 mmol) was added to a solution of N-I [trans-1-hydroxy-4- (hydroxymethyl) cyclohexyl] methyl}-4- (methoxymethoxy) benzamide (0.16 g, 0.5 mmol) in DMF (2.5 mL) and the mixture was stirred at room temperature for 1 hour. To the mixture, 2-fluorobenzylbromide (95 mg, 0.5 mmol) was added at 0 °C and the mixture was stirred overnight at room temperature. The mixture was quenched with water and diluted with AcOEt. The organic layer was washed with water and dried over MgS04. After evaporation, the residue was purified by silica gel column chlomatography (hexane: AcOEt = 2: 1) to afford N-[(trans-4-{[(2-fluorobenzyl) oxy] methyl}-1-hydroxycyclohexyl) methyl-4- (methoxymethoxy) benzamide. N-[(trans-4- { [(2-Fluorobenzyl) oxylmethyl}-1- hydroxycyclohexyl) methyl]-4- (methoxymethoxy) benzamide was dissolved in 10% HCI- MeOH (2 mL) and the mixture was stirred at 50 °C for 30 min. The mixture was evaporated.

After evaporation, the residue was purified by silica gel column chlomatography (hexane: AcOEt = 2: 1) to afford the titled compound (32 mg) as a white solid. 'H NMR (DMSO-d6) 6 : 9.95 (br, 1H), 7.94-7. 85 (m, 1H), 7.73 (d, J = 8. 7 Hz, 2H), 7.48- 7.12 (m, 4H), 6.80 (d, J = 8.7 Hz, 2H), 4.69 (br, 1H), 4.50 (s, 2H), 3.36-3. 28 (m, 4H), 1.70- 1.54 (m, 5H), 1.38-1. 10 (m, 4H) ppm.

MS (ESI) : 388. 04 (M+H) +, 386.05 (M-H)- IR (KBr) vax : 3283,2941, 1634, 1508, 1281,1223, 1084 cm-1 m. p. 174. 3 °C Example 22-A N-f (trans-4- f (2-Fluorobenzyl) oxvlmethyl-1-hydroxvcyclohexyl) methyll-4- hydroxybenzamide sodium salt This compound was prepared with N-[(trans-4-{[(2-fluorobenzyl)oxy]methyl}-1- hydroxycyclohexyl) methyl] -4-hydroxybenzamide by a procedure similar to that in Example 1-A.

'H NMR (DMSO-d6) 6 : 8. 17 (br, 1H), 7.60-7. 10 (m, 6H), 6.15 (d, J= 8.4 Hz, 2H), 4.50 (s, 2H), 3.36-3. 20 (m, 4H), 1.72-0. 98 (m, 9H) ppm.

MS (ESI): 388.05 (ES+), 386.03 (ES-) IR (KBr) Vmax : 3288, 2926,1632, 1456, 1281cm-1 Example 23 N-[(trans-4-{[(3-Fluorobenzyl)oxy]methyl}-1-hydroxycyclohexy l)methyl]-4- hydroxybenzamide This compound was prepared with 3-fluorobenzylbromide by a procedure similar to that in Example 22. 'H NMR (DMSO-d6) 8 : 9.95 (br, 1H), 7.93-7. 85 (m, 1H), 7.73 (d, J = 8.6 Hz, 2H), 7.45- 7.34 (m, 1H), 7.20-7. 04 (m, 3H), 6.80 (d, J = 8.7 Hz, 2H), 4.69 (br, 1H), 4.47 (s, 2H), 3.37- 3.27 (m, 4H), 1.73-1. 55 (m, 5H), 1. 38-1. 12 (m, 4H) ppm.

MS (ESI) : 388. 04 (M+H) +, 386.05 (M-H)- IR (KBr) Vma, : 3240,2941, 1626,1508, 1277,1117 cm~ m. p. 167. 6 °C Example 24 N-[(trans-4-{[(4-Fluorobenzyl)oxy]methyl}-1-hydroxycyclohexy l)mehtyl]-4- hydroxybenzamide This compound was prepared with 4-fluorobenzylbromide by a procedure similar to that in Example 22.

'H NMR (DMSO-d6) 6 : 9.96 (br, 1H), 7.93-7. 85 (m, 1H), 7.72 (d, J = 8. 8 Hz, 2H), 7.39- 7. 31 (m, 2H), 7.20-7. 12 (m, 2H), 6.79 (d, J = 8.8 Hz, 2H), 4.69 (br, 1H), 4.43 (s, 2H), 3.50- 3.25 (m, 4H), 1.70-1. 52 (m, 5H), 1.35-1. 10 (m, 4H) ppm.

MS (ESI) : 388.14 (M+H) +, 386.12 (M-H)- IR (KBr) Vmax : 3281,2934, 1624,1508, 1279,1225, 1101 cm m. p. 167. 6 °C Example 25 N-r (trans-4-f (4-Chlorobenzvl) oxylmethyl-1-hydroxvcyclohexyl) methyll-4- hydroxybenzamide This compound was prepared with 4-chlorobenzylbromide by a procedure similar to that in Example 22. 'H NMR (DMSO-d6) 8 : 7.92-7. 83 (m, 1H), 7.72 (d, J = 8.4 Hz, 2H), 7.44-7. 30 (m, 4H), 6.78 (d, J = 8.2 Hz, 2H), 4.70 (br, 1H), 4.44 (s, 2H), 3.40-3. 25 (m, 4H), 1.70-1. 50 (m, 5H), 1.38-1. 06 (m, 4H) ppm. (-OH was not observed) MS (ESI) : 404.13 (M+H) +, 402.04 (M-H)- IR (KBr) v : 3221, 2934,1630, 1508,1277, 1111 cm-1 m. p. 164. 1 °C Example 26 4-Hydroxy-N {[trans-1-hydroxy-4-(2-phenoxyethyl)cyclohexyl]methyl}benzam ide DIAD (0.35 mL, 1.8 mmol) was added to a mixure of N-{[trans-1-hydroxy-4-(2- hydroxyethyl) cyclohexyl] methyl}-4-(methoxymethoxy)benzamide (0.41 g, 1.2 mmol), phenol (0.17 g, 1. 8 mmol) and triphenylphosphine (0.47 g, 1. 8 mmol) in THF (5. 0 mL) at 0 °C and the mixture was stirred at room temperature for 16 hours. The mixture was diluted with CH2C12 and was washed with 2N aq. NaOH and water. The organic layer was dried over MgS04 and evaporated. The residue was purified by silica gel column chromatography (hexane-AcOEt 5: 2 to 1 : 1) to afford N-{[trans-1-hydroxy-4-(2- phenoxyethyl) cyclohexyl] methyl}-4- (methoxymethoxy) benzamide. N-{ [trans-I-Hydroxy-4- (2-phenoxyethyl) cyclohexyl]methyl}-4-(methoxymethoxy) benzamide was dissolved in 10% HCI-MeOH (12 mL) and the mixture was stirred at 50 °C for 30 min. After evaporation, the residue was purified by silica gel column chromatography (CH2CI2 : MeOH = 30 : 1) to give the titled compound (0.25 g).

'H NMR (DMSO-d6) 6 : 9.97 (br, 1H), 7.89 (t, J = 5.8 Hz, 1H), 7.74 (d, J = 8.7 Hz, 2H), 7.32-7. 22 (m, 2H), 6.96-6. 76 (m, 5H), 4.67 (br, 1H), 3.98 (t, J = 6.4 Hz, 2H), 3.40-3. 32 (m, 2H), 1. 74-1. 10 (m, IlH) ppm.

MS (ESI) : 370.12 (M+H) +, 368.11 (M-H)- IR (KBr) v, a, : 3231,2928, 1626,1508, 1283,1246, 1119cm~ m. p. 168. 7°C Example 27 N-(( trans-4-r2- (2-Fluorophenoxy) ethyll-1-hydroxycyclohexyl methyl)-4- hydroxybenzamide This compound was prepared with 2-fluorophenol by a procedure similar to that in Example 26.

IH NMR (DMSO-d6) 8 : 9.96 (br, 1H), 7.89 (t, J = 6.1 Hz, 1H), 7.74 (d, J = 8.7 Hz, 2H), 7.24-7. 16 (m, 3H), 6.96-6. 76 (m, 3H), 4.67 (br, 1H), 4.06 (t, J = 6.6 Hz, 2H), 3.40-3. 32 (m, 2H), 1. 74-1. 10 (m, 11H) ppm.

MS (ESI) : 388. 13 (M+H) +, 386.12 (M-H)- IR (KBr) vmax : 3233,2928, 1632,1508, 1281, 1113 cm~ m. p. 178. 9°C Example 28 N-({trans-4-[2-(3-Fluorophenoxy)ethyl]-1-hydroxycyclohexyl}m ethyl)-4- hydroxybenzamide This compound was prepared with 3-fluorophenol by a procedure similar to that in Example 26.

'H NMR (DMSO-d6) 8 : 9.97 (br, 1H), 7.89 (t, J = 5. 8 Hz, 1H), 7.74 (d, J = 8.7 Hz, 2H), 7.35-7. 23 (m, 1H), 6.85-6. 69 (m, 5H), 4.67 (br, 1H), 4.00 (t, J = 6.6 Hz, 2H), 3.40-3. 32 (m, 2H), 1. 74-1. 10 (m, I 1H) ppm.

MS (ESI): 388.11 (M+H) +, 386.13 (M-H)- IR (KBr) Vma, : 3238, 2930,1624, 1508,1281, 1134 cm~ m. p. 134. 5°C Example 29 N-(( trans-4-f2- (4-Fluorophenoxy) ethyll-1-hydroxycyclohexyl) methyl)-4- hydroxybenzamide This compound was prepared with 4-fluorophenol by a procedure similar to that in Example 26.

IH NMR (DMSO-d6) 8 : 9.96 (br, 1H), 7.89 (t, J = 5.6 Hz, 1H), 7.74 (d, J = 8. 7 Hz, 2H), 7.14-7. 04 (m, 2H), 6.98-6. 88 (m, 2H), 6.80 (d, J = 8. 7 Hz, 2H), 4.67 (br, 1H), 3.96 (t, J = 6. 4 Hz, 2H), 3. 40-3. 32 (m, 2H), 1.74-1. 10 (m, 11H) ppm.

MS (ESI) : 388. 13 (M+H) +, 386.11 (M-H)- IR (KBr) Va, : 3285, 2937,1634, 1508,1209, 1026 cm~ m. p. 177. 5°C Example 30 N-{[trans-4-(Benzyloxy)-1-hydroxycyclohexyl]methyl}-4-hydrox ybenzamide A mixture of N-{[4-(benzyloxy)-1-hydroxycyclohexyl] methyl}-4-{[2- (trimethylsilyl) ethoxy] methoxy} benzamide (0.49 g, 1.0 mmol) and TBAF (1.0 M in THF, 5.0 mL) was refluxed for 16 hours. The mixture was diluted with AcOEt and was washed with sat. aq. NH4CI. The organic layer was dried over MgSO4 and evaporated. The residue was purified by silica gel column chromatography (CH2CI2 : MeOH = 20 : 1) and HPLC (DAICEL CHIRALCEL OJ, hexane: EtOH = 7: 3) to give the titled compound (80 mg).

'H NMR (DMSO-d6) # : 10.01 (br, IH), 8.05-7. 97 (m, 1H), 7.73 (d, J = 8.6 Hz, 2H), 7.32- 7.20 (m, 5H), 6.79 (d, J = 8.6 Hz, 2H), 4.51 (br, 1H), 4.43 (s, 2H), 3.56-3. 49 (m, 1H), 3.26 (d, J= 6. 0 Hz, 2H), 1. 80-1. 55 (m, 6H), 1.35-1. 20 (m, 2H) ppm.

MS (ESI) : 356.18 (M+H) +, 354.17 (M-H)- IR (KBr) Vma, : 3134, 2934,1607, 1558,1508, 1279,1065 cm~ Example 31 N-{[trans-4-(4-Chlorophenoxy)-1-hydroxycyclohexyl]methyl}-4- hydroxybenzamide and Example 32 N-f [cis-4- (4-Chlorophenoxy)-1-hydroxycvclohexyll methyl -4-hydroxybenzami de A mixture of 4-hydroxybenzoic acid (0.55 g, 4.0 mmol), 1- (aminomethyl)-4- (4- chlorophenoxy) cyclohexanol (1.0 g, 4.0 mmol), EDCI (0.92 mg, 4. 8 mmol) and HOBt-H2O (0.74 g, 4.8 mmol) in DMF (40 mL) was stirred at room temperature for 16 hours. The mixture was diluted with AcOEt and was washed with sat. aq. NaHC03 and water. The organic layer was dried over MgS04 and evaporated. The residue was purified by silica gel column chromatography (hexane: AcOEt = 1: 3) to give the mixture of titled compounds (1.1 g). The mixture was separated by HPLC (DAICEL CHIRALPAK AD, hexane: EtOH : Et2NH = 85: 15: 0.1) to give Example 31 (0.32 g) and Example 32 (0.22 g).

Data for Example 31 : 1H NMR (DMSO-d6) 6 : 9.96 (br, 1H), 8. 02 (t, J = 5.9 Hz, 1H), 7.73 (d, J = 8.7 Hz, 2H), 7.29 (d, J = 9.0 Hz, 2H), 6.96 (d, J = 8.9 Hz, 2H), 6.80 (d, J = 8.7 Hz, 2H), 4.62-4. 46 (m, 2H), 3.30 (d, J = 5.9 Hz, 2H), 1.92-1. 30 (m, 8H) ppm.

MS (ESI) : 375.9 (M+H) +, 373.9 (M-H)- IR (KBr) Vmax : 3234,2949, 1632,1508, 1491,1283, 1238 cm-1 m. p. 199. 0 °C Data for Example 32: 'H NMR (DMSO-d6) 8 : 9.96 (br, 1H), 8.03 (t, J = 5.8 Hz, 1H), 7.74 (d, J = 8.7 Hz, 2H), 7. 28 (d, J = 9.0 Hz, 2H), 6.96 (d, J = 8.9 Hz, 2H), 6. 80 (d, J = 8.7 Hz, 2H), 4.55 (br, 1H), 4.35-4. 20 (m, 1H), 3. 26 (d, J = 6.1 Hz, 2H), 1. 88-1. 36 (m, 8H) ppm.

MS (ESI) : 375.9 (M+H) +, 373.9 (M-H)- IR (KBr) via, : 3240,2949, 1632,1508, 1491, 1281, 1240 cm m. p. 196. 6 °C Example 33 N-({[trans-4-(4-Chlorophenoxy)-1-hydroxycyclohexyl]methyl}-3 -fluoro-4- hydroxybenzamide and Example 34 N-f [cis-4-(4-Chlorophenoxe)-1-hydroxecyclohexyllmethyl}-3-fluor o-4-hydroxybenzamide These compounds were prepared with 3-fluoro-4-hydroxybenzoic by a procedure similar to that in Example 31 and 32.

Data for Example 33: 'H NMR (DMSO-d6) 8 : 8.12 (t, J = 5.9 Hz, 1H), 7.71-7. 52 (m, 2H), 7.28 (d, J= 8.9 Hz, 2H), 7.02-6. 90 (m, 3H), 4.56-4. 42 (m, 2H), 3.40-3. 20 (m, 2H), 1.92-1. 50 (m, 6H), 1.43-1. 26 (m, 2H) ppm. (-OH was not observed) MS (ESI) : 394.05 (M+H) +, 392.04 (M-H)- IR (KBr) vmax : 3350,1957, 1639,1512, 1310, 1238 cm m. p. 168. 5°C Data for Example 34: 1H NMR (DMSO-d6) 8 : 8.11 (t, J = 5.9 Hz, 1H), 7.72-7. 54 (m, 2H), 7.28 (d, J=8. 9 Hz, 2H), 7.01-6. 90 (m, 3H), 4.34-4. 20 (m, 1H), 3.26 (d, J = 6.1 Hz, 2H), 1.86-1. 36 (m, 8H) ppm.

(OH was not observed) MS (ESI) : 394.07 (M+H) +, 392.05 (M-H)- IR (KBr) v, : 3319,2941, 1618,1512, 1489,1300, 1242 cm- m. p. 168. 1 °C Examples 35-38 (+)-4-Hydroxy-N-f SS- (phenoxymethyl) tetrahvdro-2H pyran-2S-vllmethylbenzamide (-)-4-Hydroxy-N-t {[5R-(phenoxymethyl)tetrahydro-2H-pyran-2R-yl]methyl}benzami de <BR> <BR> <BR> <BR> (+)-4-Hydroxy-N-f f5R*- (phenoxymethvl) tetrahydro-2H-pyran-2S*-vllmethyllbenzamide<BR> <BR> <BR> <BR> <BR> <BR> (-)-4-Hvdroxy-N fSS(phenoxymethyl) tetrahydro-2H-pyran-2R*-yllmethyllbenzamide 4-(Methoxymethoxy)-N-{ [5-phenoxymethyl] tetrahydro-2H-pyran-2- yl} methyl} benzamide (678 mg, 1.76 mmol) was dissolved in 10#20% HCI-MeOH (5 mL) and stirred at room temperature for 2 hours. To this mixture were added H20 (50 mL) and AcOEt (50 mL). The aqueous layer was extracted with AcOEt (50 mL) and the combined organic layers were washed with sat. aq. NaHC03 (50 mL) and brine (50 mL), dried over Na2SO4, and concentrated in vacuo. The crude product was purified by silica gel column chromatography to give the mixture of the titled compounds (0.55 g, 92%). 4 stereoisomers were separated by Chiral column (Chiralpak AD-H, 20 mm I. D. x 250 mm (No. ADHOCJ- DE003), DAICEL) using n-Hexane: 2-Propanol : Et2NH = 90: 10: 0.1 as an eluent (Flow rate: 10 mL/min).

Data for Example 35: Sticky colorless solid, 99% ee, cis isomer, retention time 33 min 'H NMR (300 MHz, DMSO) 8 : 8.26-8. 22 (m, 1H), 7.73-7. 69 (m, 2H), 7. 31-7. 25 (m, 2H), 6.97-6. 90 (m, 3H), 6.80-6. 75 (m, 2H), 4.17-4. 11 (m, 1H), 4.10-3. 90 (m, 2H), 3.56-3. 44 (m, 2H), 3.29-3. 19 (m, 2H), 1.95 (bs, 1H), 1.87-1. 67 (m, 2H), 1.50-1. 30 (m, 2H) ppm. (-OH was not observed) MS (ESI) : 342.1 (M+H) +, 340.1 (M-H)- [a] D = + 12.00 (c = 0.10, MeOH, 26 °C) Data for Example 36: Sticky colorless solid, 99% ee, cis isomer, retention time 36 min IH NMR (300 MHz, DMSO) 6 : 8. 25-8.22 (m, 1H), 7.72-7. 69 (m, 2H), 7.31-7. 26 (m, 2H), 6.97-6. 90 (m, 3H), 6.78-6. 76 (m, 2H), 4.16-4. 11 (m, 1H), 4.04-3. 90 (m, 2H), 3.55-3. 47 (m, 2H), 3.27-3. 23 (m, 2H), 1.95 (bs, 1H), 1.86-1. 69 (m, 2H), 1.49-1. 23 (m, 2H) ppm. (OH was not observed) MS (ESI) : 342.1 (M+H) +, 340.1 (M-H)- [a] D =-20. 00 (c = 0.04, MeOH, 26 °C) Data for Example 37: Re-crystalized from IPA/IPE ; white solid, >99% ee, trans, retention time 47 min IH NMR (300 MHz, CDC13) 8 : 7.71-7. 61 (m, 2H), 7.31-7. 25 (m, 2H), 6.70-6. 85 (m, 5H), 6.53 (bs, 1H), 6.22 (bs, 1H), 4.23-4. 18 (m, 1H), 3.85-3. 69 (m, 3H), 3.52-3. 46 (m, 1H), 3.30- 3.21 (m, 2H), 2.15-2. 11 (m, 1H), 1.98-1. 95 (m, 1H), . 78-1.74 (m, 1H), 1. 48-1. 36 (m, 2H) ppm.

MS (ESI) : 342.1 (M+H) +, 340.1 (M-H)- [a] D = + 28. 9 (c=0. 18, MeOH, 26 °C) mp = 152. 1 °C IR (KBr) = 3355.9, 2935.5, 1635.5, 1508.2, 1226.6, 1074.3 cm~ Data for Example 38: Recrystallized from IPA/IPE; white solid; 99% ee, trans, retention time 51 min 'H NMR (300 MHz, CDC13) 8 : 7.70-7. 67 (m, 2H), 7.30-7. 27 (m, 2H), 6.97-6. 85 (m, 5H), 6.60-6. 35 (m, 2H), 4.23-4. 19 (m, 1H), 3.85-3. 70 (m, 3H), 3.50-3. 46 (m, 1H), 3.30-3. 21 (m, 2H), 2.12 (bs, 1H), 1. 98-1. 95 (m, 1H), 1.77-1. 73 (m, 1H), 1.52-1. 36 (m, 2H) ppm.

MS (ESI): 342.1 (M+H) +, 340.1 (M-H)- [a] D =-25. 3 (c = 0. 19, MeOH, 26 °C) mp=152.4 °C IR (KBr) = 3355.9, 2935.5, 1635.5, 1508.2, 1226.6, 1074.3 cm~ Example 39-42 4-Hvdroxv-N {[5S-(benzyloxymethyl)tetrahydro-2H-pyran-2S-yl]mthyl} benzamide 4-Hydroxv-N-f r5R- (benzvloxvmethvl) tetrahvdro-2H-pvran-2R-vllmethyl) benzamide<BR> <BR> <BR> <BR> <BR> <BR> 4-Hydroxy-N-T r5R*- (benzyloxymethvl) tetrahydro-2H-pyran-2S*-yllmethyl benzamide<BR> <BR> <BR> <BR> <BR> <BR> <BR> 4-Hydroxy-N-f r55*-(benzyloxymethyl) tetrahydro-2H-pyran-2R*-yllmethyl Wbenzamide<BR> <BR> <BR> <BR> <BR> <BR> <BR> 4-(benzyloxymethoxy)-N-{ [5-phenoxymethyl] tetrahydro-2H-pyran-2- yl} methyl} benzamide (1.6 g, 4.0 mmol) was dissolved in 10-20% HCl-MeOH (10 mL) and stirred at room temperature for 2 h. To the mixture were added H20 (50 mL) and AcOEt (50 mL). The aqueous layer was extracted with AcOEt (50 mL) and the combined organic layers were washed with sat. NaHC03 (50 mL), brine (50 mL), dried over Na2S04, and concentrated in vacuo. The crude product was purified by silica gel column chromatography to give the mixture of the titled compounds (1.20 g, 83%). 4 stereoisomers were separated by Chiral column (Chiralpak AD-H, 20 mm I. D. x 250 mm (No. ADHOCJ- DE003), DAICEL) using n-Hexane/2-Propanol/Et2NH = 85: 15: 0.1 as an eluent (10 mL/min).

Data for Example 39: colorless amorphous, >99% ee, cis isomer, retention time 18 min IH NMR (300 MHz, DMSO) b : 8. 25-8.21 (m, 1H), 7.72-7. 69 (m, 2H), 7.38-7. 25 (m, 5H), 6.80-6. 76 (m, 2H), 4.50 (d, J= 12.3 Hz, 1H), 4.45 (d, J= 12.3 Hz, 1H), 3.83-3. 79 (m, 1H), 3.61-3. 56 (m, 1H), 3.48-3. 43 (m, 3H), 3.28-3. 15 (m, 2H), 1.77-1. 59 (m, 3H), 1.44-1. 41 (m, 1H), 1.32-1. 19 (m, 1H) ppm. (OH was not observed) MS (ESI) : 356.1 (M+H)+, 354.1 (M-H)-.

Data for Example 40: colorless amorphous; >99% ee, cis isomer; retention time 21 min IH NMR (300 MHz, DMSO) 8 : 8. 25-8. 21 (m, 1H), 7.72-7. 69 (m, 2H), 7.38-7. 25 (m, 5H), 6.78-6. 76 (m, 2H), 4.50 (d, J= 12.0 Hz, 1H), 4.45 (d, J = 12.0 Hz, 1H), 3.83-3. 79 (m, 1H), 3.61-3. 56 (m, 1H), 3.48-3. 43 (m, 3H), 3.28-3. 17 (m, 2H), 1.77-1. 59 (m, 3H), 1.45-1. 41 (m, 1H), 1.32-1. 19 (m, 1H) ppm. (OH was not observed) MS (ESI): 356.1 (M+H) +, 354.1 (M-H)-.

Data for Example 41: colorless amorphous, >99% ee, trans isomer, retention time 34 min IH NMR (300 MHz, DMSO) 6 : 8.26-8. 22 (m, 1H), 7.71 (d, J = 8. 4 Hz, 2H), 7.37-7. 25 (m, 5H), 6.77 (d, J = 8.4 Hz, 2H), 4.45 (d, J = 12.6 Hz, 1H), 4.40 (d, J = 12.6 Hz, 1H), 3.97- 3.94 (m, 1H), 3. 27-3. 21 (m, 5H), 3.10-3. 03 (m, 1H), 1.79 (bs, 2H), 1.67-1. 63 (m, 1H), 1.18 (bs, 2H) ppm. (OH was not observed) MS (ESI) : 356.1 (M+H) +, 354.1 (M-H)- Data for Example 42: colorless amorphous; 98% ee, trans isomer; retention time 38 min 1H NMR (300 MHz, DMSO) § : 8. 32-8.22 (m, 1H), 7.72 (d, J = 8.7 Hz, 2H), 7.38-7. 24 (m, 5H), 6.77 (d, 7 = 8. 7 Hz, 2H), 4.45 (d, J = 12. 9 Hz, 1H), 4.41 (d, J = 12. 9 Hz, 1H), 3.97- 3.94 (m, 1H), 3.27-3. 17 (m, 5H), 3.10-3. 03 (m, 1H), 1.79 (bs, 2H), 1.67-1. 64 (m, 1H0, 1.18 (bs, 2H) ppm. (-OH was not observed) MS (ESI) : 356.1 (M+H) +, 354.1 (M-H)-.

Examples 43-46 (-)-4-Hydroxy-N-{[(3R,6S)-6-(phenoxymethyl)tetrahydro-2H-pyr an-3-yl]methyl}benzamide (+)-4-Hydroxy-N-{[(3R,6S)-6-(phenoxymethyl)tetrahydro-2H-pyr an-3- yllmethel ibenzamide (+)-4-Hydroxy-N-{[(3R,6R)-6-(phenoxymethyl)tetrahydro-2H-pyr an-3- vllmethvl Wbenzamide (-)-4-Hvdroxy-N-f {[(3S,6S)-6-(phenoxymethyl)tetrahydro-2H-pyran-3-yl]methyl}b enzamide A mixture of to {L6-(phenoxymethyl) tetrahydro-2H-pyran-3-ylJmethyl} amine (0.13 g), 4-hydroxybenzoic acid (83 mg, 0.60 mmol), HOBt-H2O (0. 11 g, 0.72 mmol) and EDCI (0.14 g, 0.72 mmol) in DMF (3.0 mL) was stirred at room temperature for 16 hours. The mixture was diluted with AcOEt and was washed with sat. aq. NaHC03 and water, dried over MgSO4 and evaporated. The residue was dissolved with MeOH (3 mL) and 2N aq.

NaOH (3 mL). The mixture was stirred for 2 hours and neutralized with 2 N aq. HCI (3 mL).

The whole was extracted with AcOEt. The extract was washed with sat. aq. NaHC03 and water, dried over MgSO4, and evaporated. The residue was purified by prep. TLC (hexane: AcOEt = 1: 2) to give the mixture of titled compounds. 4 stereoisomers were separated by chiral HPLC (DAICEL Chiralpak AD-H, hexane/EtOH/Et2NH = 85/15/0.1).

Data for Example 43 colorless amorphous, >99% ee, cis isomer, retention time 25 min 'H NMR (CDCI3) 8 : 7.67 (d, J = 8. 6 Hz, 2H), 7.31-7. 24 (m, 3H), 6. 98-6. 82 (m, 4H), 6.47- 6.37 (m, 1H), 5. 89 (br, 1H), 4.05-3. 48 (m, 7H), 2.04-1. 50 (m, 5H) ppm.

MS (ESI) : 342.12 (M+H) + IR (KBr) 3250,2934, 2860,1607, 1587,1508, 1454,1242 cm~ Isomer 1: [a] D =-7. 2 (c = 0.25, MeOH) Data for Example 44 colorless amorphous, >99% ee, cis isomer, retention time 27 min 'H NMR (CDC13) 8 : 7.67 (d, J = 8. 6 Hz, 2H), 7.31-7. 24 (m, 3H), 6. 98-6. 82 (m, 4H), 6.47- 6.37 (m, 1H), 5.73 (br, 1H), 4.05-3. 48 (m, 7H), 2.04-1. 50 (m, 5H) ppm.

MS (ESI) : 342.13 (M+H) + IR (KBr) 3250,2934, 2860,1607, 1587, 1508, 1454,1242 cm~ Isomer 2: [a] D = + 8.8 (c = 0.25, MeOH) Data for Example 45 colorless amorphous, >99% ee, trans isomer, retention time 59 min 'H NMR (DMSO-d6) 8 : 9.94 (br, 1H), 8. 25-8.15 (m, 1H), 7.67 (d, J = 8.7 Hz, 2H), 7. 31- 7.23 (m, 2H), 6.96-6. 88 (m, 3H), 6.78 (d, J = 8.7 Hz, 2H), 3.96-3. 86 (m, 3H), 3.65-3. 50 (m, 1H), 3.16-3. 00 (m, 3H), 1.93-1. 65 (m, 3H), 1.45-1. 10 (m, 2H) ppm.

MS (ESI) : 342.14 (M+H) +, 340.12 (M-H)-.

Isomer 3: [a] D = + 2.4 (c = 0.25, MeOH) Data for Example 46 colorless amorphous, >99% ee, trans isomer, retention time 71 min 'H NMR (DMSO-d6) 8 : 9.93 (br, 1H), 8.25-8. 15 (m, 1H), 7.67 (d, J = 8. 7 Hz, 2H), 7.31- 7.23 (m, 2H), 6.96-6. 88 (m, 3H), 6.78 (d, J = 8.7 Hz, 2H), 3.96-3. 86 (m, 3H), 3.65-3. 50 (m, 1H), 3.16-3. 00 (m, 3H), 1.93-1. 65 (m, 3H), 1.45-1. 10 (m, 2H) ppm.

MS (ESI) : 342.13 (M+H) +, 340.10 (M-H)-.

Isomer 3: [a] p =-3.4 (c = 0.50, MeOH) Example 47 N-(f trans-4-f (4-Fluorobenzyl) oxyl-1-hydroxycyclohexvl} methyl)-4-hydroxvbenzamide To a solution of 4-1 [ (14- [ (4-fluorobenzyl) oxy]-l- hydroxycyclohexyl} methyl) amino] carbonyl} phenyl acetate (4.0 g, 9.6 mmol) in MeOH (20 ml) and THF (20 ml) was added 2N-NaOH aq. (9.6 ml) at 0°C and the mixture was stirred at the same temperature for 2 hr. The reaction mixture was adjusted to pH 4.0 with 2N-HC1 aq. The solvent was removed in vacuo. The residue was extracted with ethyl acetate (50 ml x 3). The combined organic layer was dried over Na2S04, and concentrated in vacuo. The residue was purified by column chromatography on silica gel (dichloromethane: methanol = 20: 1 as eluent) and HPLC to afford the titled compound as a white solid (248 mg, 7%).

IH NMR (DMSO-d6) 8 : 9.97 (br, 1H), 8.02-7. 98 (m, 1H), 7.74-7. 71 (m, 2H), 7.35-7. 30 (m, 2H), 7.12-7. 06 (m, 2H), 6.81-6. 78 (m, 2H), 4.50 (br, 1H), 4.41 (s, 2H), 3.52 (br, 1H), 3.26 (d, J = 6.0 Hz, 2H), 1.79-1. 58 (m, 6H), 1.32-1. 26 (m, 2H) ppm.

MS (ESI): 374.12 (M+H) +, 372.12 (M-H)- IR (KBr) vm"Ç : 2932,1703, 1508,1227, 1084, 826 cm-1 Anal. Calcd. for C2sH24NO4F : C, 67.54 ; H, 6.48 ; N, 3.75. Found: C, 67.43 ; H, 6.47 ; N; 3.70 m. p. 122. 1 °C, 160. 9 °C Example 48 N ({trans-4-[(2-Fluorobenzyl)oxy]-1-hydroxycyclohexyl}methyl)- 4-hydroxybenzamide This compound was prepared with 4-{[({4-[(2-fluorobenzyl)oxy]-1- hydroxycyclohexyl} methyl) amino] carbonyl} phenyl acetate by a procedure similar to that in Example 47 as a white solid.

'H NMR (DMSO-d6) 6 : 9.96 (br, 1H), 8. 01-7. 97 (m, 1H), 7.73-7. 70 (m, 2H), 7.44-7. 29 (m, 2H), 7. 18-7. 10 (m, 2H), 6.80-6. 77 (m, 2H), 4.50-4. 48 (m, 3H), 3.55 (br, 1H), 3.26 (d, J = 5.9 Hz, 2H), 1.80-1. 57 (m, 6H), 1.31-1. 27 (m, 2H) ppm.

MS (ESI) : 374. 08 (M+H) +, 372.04 (M-H)- IR (KBr)vmax: 3142,1607, 1234,1067, 763 cm~ Anal. Calcd. for C2lH24NO4F : C, 67.54 ; H, 6.48 ; N, 3.75. Found: C, 67.32 ; H, 6.58 ; N; 3. 78 m. p. 162. 9 °C, 179. 9 °C Example 49 3-Fluoro-N trans-4-[(3-fluorobenzel) oxv-1-hydroxycyclohexyllmethYl}-4- hydroxybenzamide This compound was prepared with 4-hydroxy-3-fluorobenzoic acid and 1- (aminomethyl)-4-[(3-fluorobenzyl)oxy]cyclohexanol by a procedure similar to that in Example 8 as a white solid.

'H NMR (DMSO-d6) 8 : 8. 09-8. 07 (m, 1H), 7.69-7. 68 (m, 1H), 7.57-7. 54 (m, 1H), 7.37-7. 29 (m, 1H), 7.15-7. 04 (m, 3H), 7.00-6. 93 (m, 1H), 4.46 (s, 2H), 3.53 (br, 1H), 3.28-3. 26 (m, 2H), 1. 81-1. 58 (m, 6H), 1.32-1. 27 (m, 2H) ppm.

[PhOH and OH proton were not observed.] MS (ESI): 392.05 (M+H) +, 390.03 (M-H)- IR (KBrav , ax : 2934, 1589, 1110, 785cm Anal. Calcd. for C21H23NO4F2 : C, 64.44 ; H, 5.92 ; N, 3.58. Found: C, 64. 12 ; H, 5.95 ; N, 3.61 m. p. 138. 2 °C Example 50 3-Fluoro-N-[(trans-4-{[(3-fluorobenzyl)oxy]methyl}-1-hydroxy cyclohexyl)methyl]- hydroxybenzamide This compound was prepared with 4-hydroxy-3-fluorobenzoic acid and trans-1- (aminomethyl)-4-{[(3-fluorobenzyl) oxy] methyl} cyclohexanol by a procedure similar to that in Example 8 as a white solid.

'H NMR (DMSO-d6) 8 : 10.48 (br, 1H), 8.04-8. 00 (m, 1H), 7.71-7. 66 (m, 1H), 7.59-7. 55 (m, 1H), 7.43-7. 35 (m, 1H), 7.17-7. 06 (m, 3H), 7.01-6. 96 (m, 1H), 4.62 (br, 1H), 4.47 (s, 2H), 3.34 (m, 2H), 3.29 (d, J = 6.0 Hz, 2H), 1.64-1. 60 (m, 5H), 1.33-1. 16 (m, 4H) ppm.

MS (ESI) : 406.07 (M+H) +, 404.09 (M-H)- IR (KBr) Vmax : 3179,1638, 1516,1298, 1094 cm-1 Anal. Calcd. for C22H2sNO4F2 : C, 65.17 ; H, 6.22 ; N, 3.45. Found: C, 65.14 ; H, 6.24 ; N; 3.47 m. p. 132. 4 °C Example 51 3-Fluoro-N-({trans-4-[2-(2-fluorophenoxy)ethyl]-1-hydroxycyc lohexyl}methyl)-4- hydroxybenzamide This compound was prepared with 4-hydroxy-3-fluorobenzoic acid and trans-1- (aminomethyl)-4- [2- (2-fluorophenyl) ethyl [cyclohexanol by a procedure similar to that in Example 8 as a white solid.

'H NMR (DMSO-d6) 5 : 10.46 (br, 1H), 8.04-8. 02 (m, 1H), 7.72-7. 71 (m, 1H), 7.67-7. 57 (m, 1H), 7.22-6. 90 (m, 5H), 4.59 (br, 1H), 4.09-4. 04 (m, 2H), 3.37 (m, 2H), 1.68-1. 16 (m, 11H) ppm.

MS (ESI) : 406.05 (M+H) +, 404.02 (M-H)- IR (KBr) Vmax : 3217, 2928, 1634, 1508, 1285, 1113cell Anal. Calcd. for C22H2sNo4F2 : C, 65.17 ; H, 6.22 ; N 3.45,. Found: C, 64.98 ; H, 6. 18 ; N 3.46 ; m. p. 184. 7°C Example 52 and 53 Cis- and Trans- N-{[4-(4-chlorophenoxy)cyclohexyl]methyl}-3-fluoro-4-hydroxy benzamide A mixture of 4- (azidomethyl) cyclohexyl 4-chlorophenyl ether (3.0 g, 11 mmol) and 10 % Pd/C (0.3 g) in MeOH (50 mL) was stirred under H2 atmosphere at rt. After 14 h, the mixture was filtered through a pad of celite and washed with MeOH (50 mL) and concentrated in vacuo. The residue (0.76 g out of 2.5 g,-3. 4 mmol) was dissolved in DMF (20 mL) and to this were added 3-fluoro-4-hydroxybenzoic acid (0.5 g, 3.2 mmol), WSC (0.73 g, 3.8 mmol), HOBt (0.58 g, 3. 8 mmol) and Et3N (0.90 mL, 6.4 mmol) at rt. After 18 h, the reaction mixture was quenched by addition of sat. aq. NaHC03 (50 mL) and diluted with AcOEt (50 mL). The aqueous layer was extracted with AcOEt (50 mL x 2) and the combined organic layer was washed with H2O (50 mL x 2) and brine (50 mL), dried over MgS04, filtered and concentrated. The residue was dissolved in MeOH (15 mL) and to this solution was added 2N NaOH (10 mL) and the mixture was stirred at rt. After 2 h, to this was added sat. aq. NaHC03 (50 mL) and extracted with AcOEt (100 mL). The aqueous layer was extracted with AcOEt (50 mL) and the combined organic layer was washed with brine (50 mL), dried over Na2S04, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography (hexane: AcOEt = 2: 1-1. 5: 1) and HPLC saparation to give 3-fluoro-4-hydroxy-N [ (4-phenoxycyclohexyl) methyl] benxamide (94.7 mg, 8% over 2 steps) and N-{[4-(4-chlorophenoxy) cyclohexyl] methyl}-3-fluoro-4- hydroxybenzamide (80.7 mg, 6% over 2 steps).

The cisltrans separation of N-{[4-(4-chlorophenoxy)cyclohexyl]methyl}-3-fluoro-4- hydroxybenzamide was carried out by chiral column (Chiralcel OJ, 20 mm I. D. x 250 mm (No. 53-03-20910), DAICEL) using n-hexane: EtOH: Et2NH = 79: 21: 0.1 as an eluent (Flow rate = 7 mL/min) at 40 °C.

Example 52 white solid. , 99% de, trans isomer, retention time 24 min.

IH NMR (300 MHz, CDCI3) 8 : 7.59 (dd, J = 11. 1, 2.1 Hz, 1H), 7.46-7. 42 (m, 1H), 7.24- 7. 18 (m, 2H), 7.04 (t, J = 8.4 Hz, 1H), 6.84-6. 79 (m, 2H), 6.08 (bs, 1H), 4.11 (tt, J = 10.8, 4.2 Hz, 1H), 3.34 (t, J = 6.3 Hz, 2H), 2.19-2. 15 (m, 2H), 1.94-1. 90 (m, 2H), 1.71-1. 59 (m, 1H), 1.50-1. 36 (m, 2H), 1.29-1. 07 (m, 2H) ppm. (OH was not observed.) MS (ESI) : 378.07 (M+H) +, 376.08 (M-H)- Example 53 white solid, 98% de, cis isomer, retention time 28 min.

IH NMR (300 MHz, CDC13) 8 : 7.57 (dd, J = 11. 1, 2.1 Hz, 1H), 7.44-7. 41 (m, 1H), 7.24- 7.19 (m, 2H), 7.02 (t, J = 8.4 Hz, 1H), 6. 85-6. 80 (m, 2H), 6.12 (bs, 1H), 4.49 (bs, 1H), 3.35 (t, J = 7.5 Hz, 2H), 2.06-2. 02 (m, 2H), 1.72-1. 28 (m, 7H) ppm. (OH was not observed.) MS (ESI) : 378.10 (M+H) +, 376.07 (M-H)- Example 54 N-f [cis-4-(4-Fluorophenoxy) cvolohexyllmethyl}-4-hydroxybenzamide This compound was prepared with N-{[cis-4-(4-fluorophenoxy)cyclohexyl]methyl}-4- (methoxymethoxy) benzamide by a procedure similar to that in Example 6 as a white solid.

'H NMR (DMSO-d6) 8 : 9.94 (br, 1H), 8.22-8. 18 (m, 1H), 7.70 (d, J=8. 7 Hz, 2H), 7.12- 7.06 (m, 2H), 6.98-6. 93 (m, 2H), 6.78 (d, J = 8.7 Hz, 2H), 4.49 (m, 1H), 3.15-3. 11 (m, 2H), 1.87-1. 84 (m, 2H), 1.65-1. 49 (m, 5H), 1.35-1. 26 (m, 2H) ppm.

MS (ESI) : 344.20 (M+H) +, 342.19 (M-H)- IR (KBr) va, : 3283,2934, 1632,1502, 1202 cm-1 Anal. Calcd. for C2oH22NO3F : C, 69.95 ; H, 6.46 ; N, 4.08. Found: C, 70.10 ; H, 6.46 ; N; 4.10. m. p. 170. 5 °C Example 55 3-Fluoro-N-f [cis-4-(4-fluorophenoxy) cyclohexyllmethyl}-4-hYdroxybenzamide This compound was prepared with 3-fluoro-4-hydroxybenzoic acid and { [4- (4- fluorophenoxy) cyclohexyl] methyl} amine by a procedure similar to that in Example 8 as a white solid.

1H NMR (DMSO-d6) 6 : 8.29 (m, 1H), 7.64-7. 52 (m, 2H), 7.12-7. 06 (m, 2H), 6.98-6. 93 (m, 4H), 4.50 (br, 1H), 3.16-3. 11 (m, 2H), 1. 87-1. 26 (m, 9H) ppm.

MS (ESI) : 362.13 (M+H) +, 360.08 (M-H)- IR (KBr) vmax: 1498,1436, 833, 760 cm-1.

Anal. Calcd. for C2oH2lNO3F2 : C, 66.47 ; H, 5.86 ; N, 3.88. Found: C, 66.35 ; H, 5.83 ; N; 3.90. m. p. 149. 5 °C Example 56 N-({trans-4-[2-(2-Fluorophenoxy)ethyl]-1-hydroxycyclohexyl}m ethyl)-1H-pyrazole-4- carboxamide This compound was prepared with lH-pyrazole-4-carboxylic acid and trans-l- (aminomethyl)-4- [2- (2-fluorophenyl) ethyl [cyclohexanol by a procedure similar to that in Example 8 as a white solid.

'H NMR (DMSO-d6) 8 : 13.11 (br, 1H), 8.08 (br, 2H), 7.82-7. 78 (m, 1H), 7.23-7. 08 (m, 3H), 6. 95-6. 87 (m, 1H), 4.59 (br, 1H), 4.09-4. 04 (m, 2H), 3.31 (m, 2H), 1.68-1. 16 (m, 11H) ppm.

MS (ESI): 362.18 (M+H) + IR (KBr) vmax 3173,2924, 1636, 1508, 1283,746 cm Anal. Calcd. for Cl9H24N303F : C, 63.14 ; H, 6.69 ; N, 11. 63. Found: C, 62.99 ; H, 6.63 ; N; 11.61 m. p. 175. 1 °C Example 57 N-{[trans-1-Hydroxy-4-(phenoxymethyl)cyclohexyl]methyl}-2-ox o-2,3-dihydro-1,3- benzoxazole-6-carboxamide This compound was prepared with 2-oxo-2, 3-dihydro-1, 3-benzoxazole-6-carboxylic acid by a procedure similar to that in Example 8 as a white solid.

'H NMR (DMSO-d6) 5 : 11.92 (br, 1H), 8.20-8. 11 (m, 1H), 7.81 (s, 1H), 7.78-7. 70 (m, 1H), 7.30-7. 21 (m, 2H), 7.15 (d, J= 8.1 Hz, 1H), 6.96-6. 86 (m, 3H), 4.65 (s, 1H), 3.82 (d, J= 6. 1Hz, 2H), 3.45-3. 35 (m, 2H), 1.83-1. 60 (m, SH), 1.40-1. 20 (m, 4H) ppm.

MS (ESI): 397.22 (M+H) +, 395.21 (M-H)- IR (KBr) vmax 2934,1763, 1601,1495, 1240, 754 cm Example 58 4-Hydroxy-N-{[cis-4-(2-phenylethoxy)cyclohexyl]methyl}benzam ide This compound was prepared with {[cis-4-(2-Phenylethoxy)cyclohexyl]methyl} amine by a procedure similar to that in Example 8 as a white solid.

'H NMR (DMSO-d6) 8 : 9.92 (br, 1H), 8. 20-8.11 (m, 1H), 7.70 (d, J=8. 7Hz, 2H), 7.32- 7.14 (m, 5H), 6.78 (d, J = 8. 7 Hz, 2H), 3.60-3. 40 (m, 3H), 3.10-3. 00 (m, 2H), 2.85-2. 75 (m, 2H), 1. 80-1. 10 (m, 9H) ppm.

IR (KBr) Vmax : 2922,1541, 1277, 1238, 1175,754 cm Example 59 2-Fluoro-4-hydroxy-N-{[trans-1-hydroxy-4-(phenoxymethyl)cycl ohexyl]methyl}benzamide This compound was prepared with 2-fluoro-4-hydroxybenzoic acid by a procedure similar to that in Example 8 as a white solid.

'H NMR (DMSO-d6) 6 : 10.47 (br, 1H), 7.76-7. 45 (m, 2H), 7.31-7. 21 (m, 2H), 6.96-6. 85 (m, 3H), 6.72-6. 55 (m, 2H), 4.68 (s, 1H), 3. 82 (d, J = 6.2Hz, 2H), 3.45-3. 35 (m, 2H), 1.83-1. 60 (m, 5H), 1.40-1. 20 (m, 4H) ppm.

MS (ESI): 374.23 (M+H) +, 372.24 (M-H)- IR (KBr)vmax : 3200,2938, 1495,1227, 847, 768 cm~ Example 60 N-( (trans-4-r (Benzvloxy) methyll-1-hvdroxvcyclohexvlyl)-3-fluoro-4- hydoxybenzamide This compound was prepared with 3-fluoro-4-hydroxybenzoic acid and trans-1- (aminomethyl)-4-[(benzyloxy) methyl] cyclohexanol hydrochloride by a procedure similar to that in Example 8.

1H NMR (300 MHz, DMSO) 8 : 8.01 (m, 1H), 7.70-7. 55 (m, 2H), 7.37-7. 24 (m, 5H), 6.98 (t, J = 8.61 Hz, 1H), 4. 44 (s, 2H), 3.29-3. 27 (m, 4H), 1.63-1. 60 (m, 5H), 1.32-1. 15 (m, 4H) ppm. (OH was not observed.) MS (ESI) : 386. 16 (M-H)- mp = 162. 5 °C.

IR (KBr) vmax : 3355. 9,2945. 1,1635. 5,1517. 9,1299. 9,1093. 6 cm.

Anal. Calcd for C22H26NO4F : C, 68.20, H, 6.76, N, 3.62, O, 16.52, F, 4.90. Found : C, 68.12, H, 6.93, N, 3.63.

Example 61 N-(( cis-4-f (Benzvloxy methyllcvclohexvl) methvl)-4-hydroxybenzamide This compound was prepared with ({cis-4-[(benzyloxy) methyl] cyclohexyl} methyl) amine by a procedure similar to that in Example 8.

IH NMR (CDC13) 8 : 7.65 (d, J = 8.7 Hz, 2H), 7.36-7. 24 (m, 5H), 6.86 (d, J = 8.7 Hz, 2H), 6.48 (s, 1H), 6.10-6. 00 (m, 1H), 4.50 (s, 2H), 3.44-3. 35 (m, 4H), 1.95-1. 35 (m, 10H) ppm.

MS (ESI) : 354.23 (M+H) +, 352.23 (M-H)- Example 62 3-Fluoro-4-hydroxy-N-f trans-1-hydroxy-4- (phenoxvmethyl) cvclohexvllmethyl} benzamide This compound was prepared with 3-fluoro-4-hydroxybenzoic acid by a procedure similar to that in Example 8.

'H NMR (DMSO-d6) 8 : 10.46 (br, 1H), 8.04 (t, J = 5.9 Hz, 1H), 7.73-7. 55 (m, 2H), 7.30- 7.22 (m, 2H), 7.02-6. 88 (m, 4H), 4.63 (br, 1H), 3.82 (d, J = 6.0 Hz, 2H), 3.37 (d, J = 6.0 Hz, 2H), 1. 86-1. 58 (m, 5H), 1.40-1. 20 (m, 4H) ppm.

MS (ESI) : 374.04 (M+H) +, 372.03 (M-H)- IR (KBr) vax : 3296,2934, 1499,1242 cm-1 m. p. 183. 5 °C Example 63 3-Fluoro-4-hydroxy-N- rtrans-1-hydroxy-4- (2-phenoxyethvl) cyclohexyllmethyl} benzamide This compound was prepared with 3-fluoro-4-hydroxybenzoic acid and trans-1- (aminomethyl)-4- (2-phenoxyethyl) cyclohexanol hydrochloride by a procedure similar to that in Example 8.

'H NMR (DMSO-d6) 8 : 10.45 (br, 1H), 8.01 (t, J = 5.9 Hz, 1H), 7.74-7. 54 (m, 2H), 7.32- 7.22 (m, 2H), 7.03-6. 86 (m, 4H), 4.59 (br, 1H), 3.98 (t, J = 6.4 Hz, 2H), 3. 36 (d, J= 6.4 Hz, 2H), 1. 74-1. 10 (m, 11 H) ppm.

MS (ESI) : 388. 14 (M+H) +, 386.16 (M-H)- IR (KBr) Vmax : 3227,2956, 1520,1302 cm~ m. p. 164. 0 °C Example 64 3-Fluoro-N-[(trans-4-{[(4-fluorobenzyl)oxy]methyl}-4-hydroxy cyclohexyl)methyl]-4- hydroxybenzamide This compound was prepared with 3-fluoro-4-hydroxybenzoic acid and trans-1- (aminomethyl)-4-{[(4-fluorobenzyl)oxy]methyl}cyclohexanol hydrochloride by a procedure similar to that in Example 8.

'H NMR (DMSO-d6) 8 : 10.45 (br, 1H), 8. 01 (t, J= 5.9 Hz, 1H), 7.74-7. 54 (m, 2H), 7.40- 7.30 (m, 2H), 7.22-7. 11 (m, 2H), 7.03-6. 94 (m, 1H), 4.61 (br, 1H), 4.43 (s, 2H), 3. 38-3. 24 (m, 4H), 1.70-1. 50 (m, 5H), 1.38-1. 06 (m, 4H) ppm.

MS (ESI) : 406.12 (M+H) +, 404.13 (M-H)- IR (KBr) vma1, : 3288, 2941,1639, 1508,1298 cm-1 m. p. 155. 9 °C Example 65 3-Fluoro-N ({trans-4-[(2-fluorophenoxy)methyl]-1-hydroxycyclohexyl}meth yl)-4- hydroxybenzamide This compound was prepared with 3-fluoro-4-hydroxybenzoic acid and trans-l- (aminomethyl)-4-[(2-fluorophenoxy) methyl] cyclohexanol hydrochloride by a procedure similar to that in Example 8.

'H NMR (DMSO-d6) 8 : 10.46 (br, 1H), 8.03 (t, J= 5. 5 Hz, 1H), 7.74-7. 54 (m, 2H), 7.24- 6.88 (m, 5H), 4.63 (br, 1H), 3.90 (d, J= 6.3 Hz, 2H), 3.42-3. 32 (m, 2H), 1.95-1. 55 (m, 5H), 1.42-1. 22 (m, 4H) ppm.

MS (ESI): 392.16 (M+H) +, 390.10 (M-H)- IR (KBr) Vmax ; 2926,1562, 1508, 1307, 1250 cm-1 m. p. 194. 6 °C Example 66 3-Fluoro-N- ({trans-4-[(4-fluorophenoxy)methyl]-1-hydroxycyclohexyl}meth yl)-4- hydroxybenzamide This compound was prepared with 3-fluoro-4-hydroxybenzoic and trans-l- (aminomethyl)-4- [ (4-fluorophenoxy) methyl] cyclohexanol hydrochloride by a procedure similar to that in Example 8.

'H NMR (DMSO-d6) 6 : 10.47 (br, 1H), 8.15-7. 95 (m, 1H), 7.78-7. 53 (m, 2H), 7.20-6. 87 (m, 5H), 4.64 (br, 1H), 3.80 (d, J = 5.9 Hz, 2H), 3.50-3. 25 (m, 2H), 1.89-1. 55 (m, SH), 1.45- 1. 18 (m, 4H) ppm.

MS (ESI): 392.12 (M+H) +, 390.10 (M-H)- IR (KBr) Vmax : 3288, 2926, 1628, 1508,1299 cm-1 m. p. 181. 6 °C Example 67 4-Hydroxy-N-[(trans-1-hydroxy-4-{[(5-methylpyridin-2- yl) oxylmethyl lcyclohexyl) methyllbenzamide This compound was prepared withN-[(trans-l-hydroxy-4-{[(5-methylpyridin-2- yl) oxy] methyl}cyclohexyl)methyl]-4-(methoxymethoxy) benzamide by a procedure similar to that in Example 6.

'H NMR (DMSO-d6) 5 : 9.98 (br, 1H), 8.06-7. 85 (m, 2H), 7.73 (d, J = 8. 7 Hz, 2H), 7.55- 7.47 (m, 1H), 6.80 (d, J = 8. 7 Hz, 2H), 6.69 (d, J = 8.4 Hz, 1H), 4.71 (br, 1H), 4. 08 (d, J= 6.4 Hz, 2H), 3.45-3. 30 (m, 2H), 2.19 (s, 3H), 1.86-1. 52 (m, 5H), 1.43-1. 13 (m, 4H) ppm.

MS (ESI) : 371.10 (M+H) +, 369.08 (M-H)- IR (KBr) Vmax : 3358,2934, 1570,1512, 1277 cm m. p. 196. 2 °C Example 68 N-F (trans-4-Benzvl-1-hydroxycyclohexyl) methyll-4-hydroxybenzamide A mixture of N [ (4-benzylidene-1-hydroxycyclohexyl) methyl]-4- (benzyloxy) benzamide (42 mg) and 20% Pd (OH) 2-C (10 mg) in MeOH (5 mL) was hydrogenated at 4 atm for 10 hours. The mixture was filtered through a pad of celite and the filtrate was evaporated. The residue was purified by silica gel column chromatography (hexane: AcOEt = 1: 2), followed by HPLC (DAICEL CHIRALCEL OJ-H, hexane: EtOH: Et2NH = 85 : 15: 0.1) to give the titled compound (29 mg) as a white solid.

'H NMR (DMSO-d6) 8 : 9, 97 (br, 1H), 7.95-7. 85 (m, 1H), 7.74 (d, J = 8.6 Hz, 2H), 7.34- 7.12 (m, 5H), 6.80 (d, J= 8.6 Hz, 2H), 4.65 (s, 1H), 3.45-3. 30 (m, 2H), 1.77-1. 04 (m, 11H) ppm.

MS (ESI): 340.20 (M+H) +, 338.21 (M-H)- IR (KBr) Vma,, : 3165,2925, 1541,1508, 1285 cm Example 69 3-Fluoro-N-F (trans-4-f [(2-fluorobenzyl) oxylmethvl T-I-hydroxyceclohexyl) methyll-4- hydroxybenzamide This compound was prepared with 3-fluoro-4-hydroxybenzoic and trans-1- (aminomethyl)-4- { [ (2-fluorobenzyl) oxy] methyl} cyclohexanol by a procedure similar to that in Example 8.

IH-NMR (DMSO-d6) 6 : 8.02 (dd, J = 5.9, 5.7Hz, 1H), 7.69 (dd, J = 12.5, 2 Hz, 1H), 7.57 (dd, J=8. 4, 1. 5Hz, 1H), 7.44 (ddd, J= 7.5, 7.5, 1.6 Hz, 1H), 7.40-7. 32 (m, 1H), 7.23-7. 14 (m, 2H), 6.99 (t, J = 8.6 Hz, 1H), 4.62 (br, 1H), 4.50 (s, 2H) 3.25-3. 45 (m, 4H), 1.66-1. 57 (m, 5H), 1. 34-1. 10 (m, 4H) ppm. (OH was not observed.) Example 70 4-Hydroxy-N-{[trans-4-(phenoxymethyl)cyclohexyl]methyl}benza mide This compound was prepared with {[4-(phenoxymethyl) cyclohexyl] methyl} amine hydrochloride by a procedure similar to that in Example 8.

'H NMR (CDC13) 6 : 7.67 (d, J= 8.6 Hz, 2H), 7.30-7. 24 (m, 2H), 6.96-6. 84 (m, 5H), 6.17- 6.08 (m, 1H), 3.76 (d, J= 6.2 Hz, 2H), 3.36-2. 98 (m, 2H), 2.03-0. 98 (m, 10H) ppm. (OH was not observed.) MS (ESI) : 340.17 (ES+), 338. 15 (ES-) Example 71 6-Hvdroxy-N-(fcis-4- (2-phenethoxy) cyclohexyllmethvl) nicotinamide This compound was prepared with 6-hydroxynicotinic acid and { [cis-4- (2- phenylethoxy) cyclohexyl] methyl} amine by a procedure similar to that in Example 8 as a white solid. 'H NMR (DMSO-d6) 8 : 11.94 (br, 1H), 8.19-8. 15 (m, 1H), 7.98-7. 97 (m, 1H), 7.87-7. 83 (m, 1H), 7.30-7. 18 (m, 5H), 6.35-6. 32 (m, 1H), 3.54 (t, J = 6.9 Hz, 2H), 3.47 (br, 1H), 3.05-3. 00 (m, 2H), 2.79 (t, J = 6.9 Hz, 2H), 1.70-1. 15 (m, 9H) ppm.

MS (ESI) : 355.19 (M+H) +, 353.21 (M-H)- IR (KBr)vmax: 3314,3057, 2928,2864, 1713,1605, 1553,1310, 1090 cell Anal. Calcd. for C2lH26N203F : C, 71.16 ; H, 7.39 ; N, 7.90. Found: C, 71.15 ; H, 7.40 ; N; 7.90 m. p. 181. 8 °C Example 72 N-f {[cis-4-(2-Phenylethoxy)cyclohexyl]methyl}-1H-pyrazole-4-car boxamide This compound was prepared with and lH-pyrazole-4-carboxylic acid { [cis-4- (2- phenylethoxy) cyclohexyl] methyl} amine by a procedure similar to that in Example 8 as a white solid.

'H NMR (DMSO-d6) 6 : 13.07 (br, 1H), 8.15 (br, 1H), 8.00-7. 87 (m, 2H), 7.30-7. 18 (m, 5H), 3.54 (t, J = 6.9 Hz, 2H), 3.47 (br, 1H), 3.04-3. 00 (m, 2H), 2.81-2. 76 (m, 2H), 1.75-1. 71 (m, 2H), 1.52-1. 16 (m, 7H) ppm.

MS (ESI): 328. 25 (M+H) +, 326.19 (M-H)- IR (KBr)vmax : 2853, 1248,1090 cm-1 Anal. Calcd. for Cl9H25N302 : C, 69.70 ; H, 7.70 ; N, 12.83. Found: C, 69.64 ; H, 7.66 ; N; 12.67 m. p. 145. 0 °C Example 73 N-{[cis-4-(Phenoxymethyl)cyclohexyl]methyl}-1H-pyrazole-4-ca rboxamide This compound was prepared with lH-pyrazole-4-carboxylic acid (50 mg, 0.4 mmol) and ( { [cis-4- (phenoxymethyl) cyclohexyl] methyl} amine hydrochloride (171 mg, 0.7 mmol) by a procedure similar to that in Example 8 as a white solid (25 mg, 18%).

'H NMR (DMSO-d6) 8 : 13.08 (brs, 1H), 8.13-7. 90 (m, 3H), 7.30-7. 25 (m, 2H), 6.95-6. 88 (m, 3H), 3. 87 (d, J = 6.8 Hz, 2H), 3.20-3. 15 (m, 2H), 1.90-1. 41 (m, 10H) ppm.

MS (ESI) : 314.21 (M+H) +, 312.15 (M-H)- IR (KBr) Vmax : 3317,2926, 1626,1570, 1246,1036 cm-1 Anal. Calcd. for C18H23N302 : C, 68. 98; H, 7.40 ; N, 13.41. Found: C, 68. 68 ; H, 7.40 ; N; 13.35 m. p.: 150. 1°C Example 74 N-(f (3R, 6S)-6-r (4-Fluorophenoxv) methvlltetrahydro-2H-pyran-3-yl} methyl)-4- hydroxybenzamide N-( { (3Rs6S)-6-[(4-fluorophenoxy) methyl] tetrahydro-2H-pyran-3-yl} methyl)-4- hydroxybenzamide was prepared with ( { (3R 6S)-6- [ (4-Fluorophenoxy) methyl] tetrahydro- 2H-pyran-3-yl} methyl) amine by a procedure similar to that in Example 8. Cis stereoisomer was separated by Chiral column (Chiralpak AD-H, 20 mm I. D. x 250 mm, DAICEL) using n-Hexane/2: Propanol: Et2NH = 85 : 15: 0.1 as an eluent (10 mL/min). colorless amorphous, >99% ee, cis isomer, retention time 24 min 'H NMR (CDCI3) 8 : 7.66 (d, J = 8. 6 Hz, 2H), 7.01-6. 80 (m, 6H), 6.50-6. 25 (m, 2H), 4.08- 3.46 (m, 7H), 2.05-1. 50 (m, 5H) ppm.

MS (ESI) : 360.14 (M+H) +, 358. 15 (M-H)- IR (KBr) vmo : 3350,2936, 1609,1508, 1452,1207 cm~ [α]D=-6. 5 (c = 0.4, MeOH) Example 75 and 76 N-({(2R*,5R*)-5-[(4-Fluorophenoxy)methyl]tetrahydro-2H-pyran -2-yl}methyl)-4- hydroxybenzamide was prepared with ({ (2S*, SS*)-5-[(4-fluorophenoxy) methyl] tetrahydro- 2H-pyran-2-yl} methyl) amine by a procedure similar to that in Example 8. The enantimers were separated by Chiral column (Chiralpak OJ-H, 20 mm I. D. x 250 mm, DAICEL) using n-Hexane: Ethanol : Et2NH = 85: 15: 0.1 as an eluent (10 mL/min).

Example 75 N-({(2R,5R)-5-[(4-Fluorophenoxy)methyl]tetrahydro-2H-pyran-2 -yl}methyl)-4- hydroxybenzamide >99% ee, retention time 25 min.

'H NMR (DMSO) 6 : 9.96 (bs, 1H), 8.24 (t, J=5. 5Hz, lH), 7.72 (d, J=8. 7Hz, 2H), 7.14- 7.05 (m 2H), 7.01-6. 90 (m, 2H), 6.78 (d, J = 8. 7 Hz, 2H), 4.14-3. 75 (m, 3H), 3.53-3. 16 (m, 4H), 2.00-1. 65 (m, 3H), 1.50-1. 30 (m, 2H) ppm.

MS (ESI) : 360.14 (M+H) +, 358.15 (M-H)- IR (KBr) via, : 3350,2936, 1609,1508, 1452,1207 cm~ [ab=-10 (c=0. 4, MeOH) Example 76 N-({(2R,5R)-5-[(4-Fluorophenoxy)methyl]tetrahydro-2H-pyran-2 -yl}methyl)-4- hydroxybenzamide >99% ee, retention time 31 min.

'H NMR (DMSO) 6 : 9.96 (bs, 1H), 8. 24 (t, J = 5.5 Hz, 1H), 7.72 (d, J= 8.7 Hz, 2H), 7.14- 7.05 (m 2H), 7.01-6. 90 (m, 2H), 6. 78 (d, J= 8.7 Hz, 2H), 4.14-3. 75 (m, 3H), 3.53-3. 16 (m, 4H), 2.00-1. 65 (m, 3H), 1.50-1. 30 (m, 2H) ppm.

MS (ESI): 360.14 (M+H) +, 358. 15 (M-H)- IR (KBr) Vmax : 3350,2936, 1609,1508, 1452,1207 cm~ [α] D = +5 (c = 0.4, MeOH) Example 77 N-f [cis-4-(2-Phenoxyethel) cvclohexvllmethvl}-lH-pvrazole-4-carboxamide This compound was prepared with lH-pyrazole-4-carboxylic acid (57 mg, 0.5 mmol) and { [cis-4-(2-phenoxyethyl) cyclohexyl] methyl} amine hydrochloride (118 mg, 0.5 mmol) by a procedure similar to that in Example 8 as a white solid (50 mg, 30%).

'H NMR (DMSO-d6) S : 13.08 (brs, 1H), 8.12-7. 91 (m, 3H), 7.30-7. 24 (m, 2H), 6.93-6. 88 (m, 3H), 4.01-3. 96 (m, 2H), 3.19-3. 14 (m, 2H), 1.69-1. 42 (m, 12H) ppm.

MS (ESI) : 328. 24 (M+H) +, 326.20 (M-H)- IR (KBr) Vmax : 2924,1636, 1246,756 cm-1 Anal. Calcd. for Cl9H25N302 : C, 69.70 ; H, 7.70 ; N, 12.83. Found: C, 69.34 ; H, 7.60 ; N; 12.72 m. p.: 155. 7°C Example 78 4-Hydroxy-N- cis-4- (2-phenoxyethoxy) cyclohexyl methyl} benzamide This compound was prepared with 4-(methoxymethoxy)-N-{[cis-4-(2- phenoxyethoxy) cyclohexyl] methyl} benzamide (81 mg, 0.2 mmol) by a procedure similar to that in Example 6 as colorless amorphous (64 mg, 88%).

IH NMR (DMSO-d6) 6 : 9.94 (brs, 1H), 8.20-8. 16 (m, 1H), 7.71-7. 68 (m, 2H), 7.30-7. 25 (m, 2H), 6.95-6. 90 (m, 3H), 6.79-6. 76 (m, 2H), 4.09-4. 06 (m, 2H), 3.70-3. 67 (m, 2H), 3.57-3. 52 (m, 1H), 3.10-3. 06 (m, 2H), 1.75-1. 26 (m, 9H) ppm.

Example 79 2-Oxo-N- [cis-4-(2-phenelethoxy) cyclohexyllmethYl T-2, 3-dihydro-1 3-benzoxazole-6- carboxamide This compound was prepared with 2-oxo-2, 3-dihydro-1, 3-benzoxazole-6-carboxylic acid and { [cis-4-(2-phenylethoxy)cyclohexyl]methyl}amine hydrochloride by a procedure similar to that in Example 8 as a white solid.

'H NMR (DMSO) 6 : 8.40 (t, J= 5.5 Hz, 1H), 7.82-7. 68 (m, 2H), 7.35-7. 10 (m, 6H), 3.54 (t, J = 7. 0 Hz, 2H), 3.51-3. 43 (m, 1H), 3. 08 (t, J = 6. 2 Hz, 2H), 2.79 (t, J = 7. 0 Hz, 2H), 1.82- 1.11 (m, 9H) ppm. (NH was not observed.) Example 80 3-Fluoro-N-{[trans-4-(4-fluorobenzyl)-1-hydroxycyclohexyl]me thyl}-4-hydroxybenzamide This compound was prepared with 3-fluoro-4-hydroxybenzoic acid and trans-1- (aminomethyl)-4- (4-fluorobenzyl) cyclohexanol hydrochloride by a procedure similar to that in Example 8 as a white solid.

'H NMR (DMSO-d6) # : 10.48 (br, 1H), 8. 02 (t, J= 5.9 Hz, 1H), 7.75-7. 53 (m, 2H), 7.24- 6.94 (m, 5H), 4.59 (br, 1H), 3.40-3. 30 (m, 2H), 2.55-2. 45 (m, 2H), 1.70-1. 05 (m, 9H) ppm.

MS (ESI) : 376.17 (M+H) +, 374.22 (M-H)- IR (KBr) vmax: 3422,2930, 1643,1508, 1308,1223 cm~ Example 81 N {[trans-4-(4-Florobenzyl)-1-hydroxycyclohexyl]methyl}-4-hydr oxybenzamide This compound was prepared with trans-1- (aminomethyl)-4- (4- fluorobenzyl) cyclohexanol hydrochloride by a procedure similar to that in Example 8 as a white solid. lH NMR (DMSO-d6) 5 : 9.98 (br, 1H), 7.90 (t, J = 5.7 Hz, 1H), 7. 73 (d, J = 8.6 Hz, 2H), 7.25-7. 02 (m, 4H), 6.80 (d, J= 8.6 Hz, 2H), 4.66 (br, 1H), 3.40-3. 30 (m, 2H), 2.55-2. 45 (m, 2H), 1.70-1. 05 (m, 9H) ppm.

MS (ESI) : 358.18 (M+H) +, 356.21 (M-H)- IR (KBr) vmax : 3319,2934, 1608, 1508, 1450,1221 cm~ Example 82 6-Hydroxy-N-f [cis-4- (phenoxymethyl) cvclohexyllmethyl} nicotinamide This compound was prepared with 6-hydroxynicotinic acid (80 mg, 0.6 mmol) and { [cis- 4-(phenoxymethyl)cyclohexyl]methyl}amine hydrochloride (147 mg, 0.6 mmol) by a procedure similar to that in Example 8 as a white solid (110 mg, 56%).

IH NMR (DMSO-d6) 8 : 11.94 (brs, 1H), 8.22-8. 18 (m, 1H), 7.99-7. 98 (m, 1H), 7.89-7. 85 (m, 1H), 7.31-7. 26 (m, 2H), 6.95-6. 88 (m, 3H), 6.36-6. 32 (m, 1H), 3.87 (d, 7= 8. 1 Hz, 2H), 3.21-3. 16 (m, 2H), 1.90-1. 40 (m, 10H) ppm.

MS (ESI) : 341.17 (M+H) +, 339.19 (M-H)- IR (KBr) v,,, : 3339,2926, 1638, 1545, 1246, 1036 cm-1 Anal. Calcd. for C20H24N2O3 : C, 70.56 ; H, 7.11 ; N, 8.23. Found: C, 70.36 ; H, 7.15 ; N; 8.31 m. p. : 189. 8°C Example 83 2-Hydroxy-N-{rcis-4-(2-phenoxyethyl) cyclohexYllmethyl Wisonicotinamide This compound was prepared with 2-hydroxyisonicotinic acid (63 mg, 0.5 mmol) (Tetrahedron Lett. 1988,29, 4389) and {[cs-4-(2-phenoxyethyl) cyclohexyl] methyl} amine hydrochloride (105 mg, 0.5 mmol) by a procedure similar to that in Example 8 as a white solid (30 mg, 19%).

IH NMR (DMSO-d6) 8 : 11.78 (brs, 1H), 8.58-8. 56 (m, 1H), 7.45-7. 43 (m, 1H), 7. 30-7. 25 (m, 2H), 6.94-6. 88 (m, 3H), 6.71-6. 70 (m, 1H), 6.47-6. 44 (m, 1H), 4.00-3. 96 (m, 2H), 3.20- 3.16 (m, 2H), 1.71-1. 39 (m, 12H) ppm.

MS (ESI) : 355.11 (M+H) +, 353.17 (M-H)- IR (KBr)vmax : 2920,1639, 1244,756 cm' Anal. Calcd. for C21H26N2O3#0.1H2O : C, 70.80. ; H, 7.41 ; N, 7. 86. Found: C, 70.73 ; H, 7.17 ; N; 7. 78 m. p. : 199. 9°C Example 84 6-Hydroxy-N-f fcis-4- (2-phenoxyethyl) cyclohexyllmethvl} nicotinamide This compound was prepared with 6-hydroxynicotinic acid (63 mg, 0.5 mmol) and { [cis- 4- (2-phenoxyethyl) cyclohexyl] methyl} amine hydrochloride (105 mg, 0.5 mmol) by a procedure similar to that in Example 8 as a white solid (77 mg, 48%).

'H NMR (DMSO-d6) S : 11.93 (brs, 1H), 8.21-8. 17 (m, 1H), 7.98-7. 97 (m, 1H), 7.88-7. 84 (m, 1H), 7.30-7. 25 (m, 2H), 6.93-6. 88 (m, 3H), 6.35-6. 32 (m, 1H), 4.00-3. 96 (m, 2H), 3.19- 3.15 (m, 2H), 1.69-1. 36 (m, 12H) ppm.

MS (ESI): 355.20 (M+H) +, 353.27 (M-H)- IR (KBr) vax : 3329,2920, 1614,1246 cm' Anal. Calcd. for C2lH26N203 : C, 71.16. ; H, 7.39 ; N, 7.90. Found: C, 70.80 ; H, 7.30 ; N; 7.93 m. p.: 167. 6°C Example 85 N-{[cis-4-(2-Phenoxyethyl)cyclohexyl]methyl}-1H-pyrazole-5-c arboxamide This compound was prepared with lH-pyrazole-5-carboxylic acid (50 mg, 0.5 mmol) and {[cis-4-(2-phenoxyethyl) cyclohexyl] methyl} amine hydrochloride (105 mg, 0.5 mmol) by a procedure similar to that in Example 8 as a white solid (44 mg, 30%).

'H NMR (DMSO-d6) 8 : 13.19 (brs, 1H), 817-8.00 (m, 0. 5H), 7.86-7. 73 (m, 0. 5H), 7.30- 7.24 (m, 2H), 6.93-6. 88 (m, 3H), 6.70-6. 55 (m, 1H), 4.00-3. 96 (m, 2H), 3.22-3. 17 (m, 2H), 1.70-1. 69 (m, 4H), 1.45-1. 40 (m, 8H) ppm. (NH proton was not observed.] MS (ESI): 328.22 (M+H) +, 326.25 (M-H)- IR (KBr) vma, : 3144,2922, 1634,1556, 1250,758 crri 1 Anal. Calcd. for Cl9H25N302 : C, 69.70. ; H, 7.70 ; N, 12.83. Found: C, 69.63 ; H, 7.50 ; N; 12.71 m. p.: 130. 5°C Example 86 N-{[cis-4-(2-Phenoxyethyl)cyclohexyl]methyl}-1H-pyrazole-4-c arboxamide This compound was prepared with 1H-imidazole-4-carboxylic acid (35 mg, 0.3 mmol) and {[cis-4-(2-phenoxyethyl) cyclohexyl] methyl} amine hydrochloride (73 mg, 0.3 mmol) by a procedure similar to that in Example 8 as a white solid (50 mg, 48%).

'H NMR (DMSO-d6) 8 : 12.43 (brs, 1H), 7.85-7. 80 (m, 1H), 7.772-7. 67 (m, 1H), 7.60-7. 55 (m, 1H), 7.30-7. 25 (m, 2H), 6.94-6. 88 (m, 3H), 4.00-3. 96 (m, 2H), 3.21-3. 16 (m, 2H), 1.71- 1.69 (m, 4H), 1.49-1. 40 (m, 8H) ppm.

MS (ESI): 328.25 (M+H) +, 326.29 (M-H)- IR (KBr) vma, : 3323,2922, 1638, 1560, 1248, 754 cm-' Anal. Calcd. for Cl9H25N302 : C, 69.70. ; H, 7.70 ; N, 12. 83. Found: C, 69.57 ; H, 7.89 ; N; 12.83 m. p.: 169. 6°C Example 87 5-Chloro-6-hydroxy-N- f cis-4- (2-phenoxyethvl) cyclohexvllmethyl} nicotinamide This compound was prepared with 5-chloro-6-hydroxynicotinic acid (69 mg, 0.4 mmol) and { [cis-4-(2-phenoxyethyl)cyclohexyl]mthyl} amine hydrochloride (93 mg, 0.4 mmol) by a procedure similar to that in Example 8 as a white solid (46 mg, 30%).

'H NMR (DMSO-d6) 8 : 12.52 (brs, 1H), 8.29-8. 25 (m, 1H), 8.17-8. 16 (m, 1H), 8. 01-8. 00 (m, 1H), 7.30-7. 24 (m, 2H), 6.94-6. 88 (m, 3H), 4.00-3. 96 (m, 2H), 3.20-3. 15 (m, 2H), 1. 78- 1.63 (m, 4H), 1.49-1. 35 (m, 8H) ppm.

MS (ESI) : 389.22 (M+H) +, 387.31 (M-H)- IR (KBr) vmax : 3325,2920, 1665,1533, 1244 cm' Anal. Calcd. for C2lH25N3O3CI : C, 64.86. ; H, 6. 48 ; N, 7.20. Found: C, 64.63 ; H, 6.64 ; N; 7.06 Exampel 88 3-Fluoro-N-[(cis-4-{[(5-fluoropyridin-2-yl)oxy]methyl}cycloh exyl)methyl]-4- hydroxybenzamide This compound was prepared with 3-fluoro-4-hydroxybenzoic acid and [(cis-4-{[(5- fluoropyridin-2-yl) oxy] methyl} cyclohexyl) methyl] amine by a procedure similar to that in Example 8 as a white solid.

'H NMR (CDCl3) 8 : 7.98 (d, J = 3.0 Hz, 1H), 7.60-7. 29 (m, 3H), 7.02 (t, J = 8.6 Hz, 1H), 6.70 (dd, J = 3.6, 9.1 Hz, 1H), 6.36-6. 18 (m, 1H), 4.15 (d, J = 7.1 Hz, 2H), 3. 37 (t, J = 6.6 Hz, 2H), 2.10-1. 30 (m, 10H) ppm. (OH was not observed.) MS (ESI) : 377.17 (M+H) +, 375.26 (M-H)- Example 89 3-Fluoro-4-hvdroxy-N ((f cis-4-f (pvridin-2-yloxy) methvllcyclohexvl) methyl) benzamide This compound was prepared with 3-fluoro-4-hydroxybenzoic acid and ({cis-4-[(pyridin- 2-yloxy) methyl] cyclohexyl} methyl) amine by a procedure similar to that in Example 8 as a white solid.

'H NMR (CDC13) 8 : 8.19-8. 12 (m, 1H), 7.63-7. 40 (m, 3H), 7.03 (t, J= 8.4 Hz, 1H), 6. 91- 6. 84 (m, 1H), 6.74 (d, J= 8.2 Hz, 1H), 6.36-6. 18 (m, 1H), 4.18 (d, J= 7.3 Hz, 2H), 3.33 (t, J = 6.4 Hz, 2H), 2.12-1. 26 (m, 10H) ppm. (OH was not observed.) MS (ESI): 359.17 (M+H) +, 357.23 (M-H)- Example 90 N ({cis-4-[2-(4-Fluorophenoxy)ethoxy]cyclohexyl}methyl)-1H-pyr azole-4-carboxamide This compound was prepared with 1H-pyrazole-4-carboxylic acid and ( {4- [2- (4- fluorophenoxy) ethoxy] cyclohexyl} methyl) amine by a procedure similar to that in Example 8.

'H NMR (CDCI3) b : 7.94 (s, 2H), 7.77-6. 81 (m, 4H), 6.08 (t, J = 5.9 Hz, 2H), 4.12-4. 05 (m, 2H), 3.77-3. 70 (m, 2H), 3.65-3. 59 (m, 1H), 3.32-3. 24 (m, 2H), 1.98-1. 83 (m, 2H), 1.72-1. 35 (m, 7H) (m, 8H) ppm. (NH was not observed.) Example 91 3,5-Difluoro-4-hydroxy-N-{[cis-4-(2-phenoxyethyl)cyclohexyl] methyl}benzamide This compound was prepared with 3, 5-difluoro-4-hydroxybenzoic acid (93 mg, 0.5 mmol) (J. Fluorine. Chem. 2000, 102, 169) and {[cis-4-(2- phenoxyethyl) cyclohexyl] methyl} amine hydrochloride (125 mg, 0.5 mmol) by a procedure similar to that in Example 8 as colorless amorphous (74 mg, 35%).

1H NMR (DMSO-d6) 8 : 10. 84 (brs, 1H), 8.41-8. 36 (m, 1H), 7.58-7. 51 (m, 2H), 7.30-7. 24 (m, 2H), 6.93-6. 88 (m, 3H), 4.01-3. 96 (m, 2H), 3.23-3. 18 (m, 2H), 1.82-1. 63 (m, 4H), 1.53- 1.32 (m, 8H) ppm.

MS (ES1) : 390.20 (M+H) +, 388. 24 (M-H)- Anal. Calcd. for C22H2sNO3F2 0. lH2O : C, 67.54. ; H, 6.49 ; N, 3.58. Found: C, 67.33 ; H, 6.57 ; N; 3.59 Example 92 6-Oxo-N {[cis-4-(2-phenoxyethyl)cyclohexyl]methyl}-1,4,5,6-tetrahydr opyridazine-3- carboxamide This compound was prepared with 6-oxo-1, 4,5, 6-tetrahydropyridazine-3-carboxylic acid and { [cis-4- (2-phenoxyethyl) cyclohexyl] methyl} amine hydrochloride by a procedure similar to that in Example 8 as colorless amorphous.

'H NMR (DMSO-d6) 6 : 11.05 (s, 1H), 8.12-8. 02 (m, 1H), 7.33-7. 20 (m, 2H), 6.98-6. 86 (m, 3H), 4.04-3. 93 (m, 2H), 3.12 (d, J = 6.9 Hz, 2H), 2.72 (d, J = 8.4 Hz, 2H), 2.38 (d, J = 8.6 Hz, 2H), 1.80-1. 25 (m, 12H) ppm.

MS (ESI) : 356.33 (M-H)- Example 93 6-Oxo-N-{[cis-4-(2-phenoxyethyl)cyclohexyl]methyl}-1,6-dihyd ropyridazine-3- carboxamide This compound was prepared with 6-oxo-1, 6-dihydropyridazine-3-carboxylic acid (70 mg, 0.5 mmol) (Chem. Pharm. Bull. 1994, 42, 371) and {[cis-4-(2- phenoxyethyl) cyclohexyl] methyl} amine hydrochloride (135 mg, 0.5 mmol) by a procedure similar to that in Example 8 as a white solid (108 mg, 61%).

'H NMR (DMSO-d6) 8 : 13.39 (brs, 1H), 8.45-8. 40 (m, 1H), 7. 82 (d, J= 10.8 Hz, 1H), 7.30- 7.24 (m, 2H), 6.97-6. 88 (m, 4H), 4.00-3. 96 (m, 2H), 3.23-3. 18 (m, 2H), 1.81-1. 63 (m, 4H), 1.49-1. 33 (m, 8H) ppm.

MS (ESI) : 35434 (M+H) + IR (KBr) Vmax : 3379, 2852, 1657,1533, 1250,1007 cm' Anal. Calcd. for C20H25N3O3#0.1CH2Cl2 : C, 66.34. ; H, 6.98 ; N, 11.55. Found: C, 66. 38 ; H, 7.07 ; N; 11.25 m. p.: 177. 5°C Example 94 N-( (cis-4-f2- (2-Fluorophenoxy) ethyllcyclohexylmethvl)-1H-pvrazole-4-carboxamide This compound was prepared with 1H-pyrazole-4-carboxylic acid and ({cis-4-[2-(2- fluorophenoxy) ethyl] cyclohexyl} methyl) amine hydrochloride by a procedure similar to that in Example 8.

'H NMR (DMSO-d6) 6 : 13.08 (br, 1H), 8.29-7. 76 (m, 3H), 7.26-6. 86 (m, 4H), 4.13-4. 02 (m, 2H), 3.23-3. 10 (m, 2H), 1. 82-1. 28 (m, 12H) ppm.

MS (ESI): 346.21 (M+H) +, 344.24 (M-H)- IR (KBr) Vmax : 3361,2926, 1630,1579, 1504,1259, 1201 cm~ Example 95 N-({cis-4-[2-(4-Fluorophenoxy)ethoxy]cyclohexyl}methyl)-6-hy droxynicotinamide This compound was prepared with 6-hydroxynicotinic acid and ( {4- [2- (4- fluorophenoxy) ethoxy] cyclohexyl} methyl) amine by a procedure similar to that in Example 8.

IH NMR (DMSO) 8 : 11.94 (bs, 1H), 8.19 (t, J = 5. 8 Hz, 1H), 7.98 (d, J = 2.6 Hz, 1H), 7.86 (dd, J = 2.6, 9.6 Hz, 1H), 7.16-6. 92 (m, 4H), 6.34 (d, J = 9.6 Hz, 1H), 4.09-4. 03 (m, 2H), 3.70-3. 63 (m, 2H), 3.58-3. 50 (m, 1H), 3.10-3. 01 (m, 2H), 1. 84-1. 70 (m, 2H), 1.63-1. 16 (m, 7H) ppm.

Example 96 N (fuis-4- (2-Phenoxyethyl) cyclohexyllmethvl-lH-pyrrole-3-carboxamide This compound was prepared with 1H-pyrrole-3-carboxylic acid (44 mg, 0.4 mmol) and { [cis-4-(2-phenoxyethyl) cyclohexyl] methyl} amine hydrochloride (108 mg, 0.4 mmol) by a procedure similar to that in Example 8 as a yellow solid (33 mg, 25%).

'H NMR (DMSO-d6) 8 : 11.07 (brs, 1H), 7.69-7. 65 (m, 1H), 7.30-7. 24 (m, 3H), 6.94-6. 88 (m, 3H), 6.73-6. 71 (m, 1H), 6.47-6. 44 (m, 1H), 4.01-3. 96 (m, 2H), 3.16-3. 12 (m, 2H), 1.74- 1.64 (m, 4H), 1.49-1. 34 (m, 8H) ppm.

MS (ESI) : 327.26 (M+H) +, 325.28 (M-H)- IR (KBr)vmax: 3204,2924, 1609,1568, 1246,754 cm-1 Anal. Calcd. for C20H26N202: C, 73.59. ; H, 8.03 ; N, 8.58. Found: C, 73.24 ; H, 7.93 ; N; 8.34 m. p.: 121. 0°C Example 97 2-Oxo-N {[cis-4-(2-phenoxyethyl)cyclohexyl]methyl}indoline-5-carboxy amide This compound was prepared with 2-oxoindoline-5-carboxylic acid and { [cis-4- (2- phenoxyethyl) cyclohexyl] methyl} amine hydrochloride by a procedure similar to that in Example 8.

IH NMR (DMSO-d6) 8 : 10.60 (br, 1H), 8.32-8. 21 (m, 1H), 7.77-7. 68 (m, 2H), 7.34-7. 22 (m, 2H), 7.00-6. 80 (m, 4H), 4.06-3. 93 (m, 2H), 3.53 (s, 2H), 3.27-3. 16 (m, 2H), 1.85-1. 29 (m, 12H) ppm.

MS (ESI): 393.32 (M+H) +, 391.37 (M-H)- IR (KBr) Vmax : 3374,2920, 1686, 1618,1489, 1292,1244 cm~ Example 98 2-Oxo-N-{ [cis-4-(2-phenoxyethyl) cyclohexyl] methyl}-1, 2,3, 4-tetrahydroquinoline-6- carboxamide This compound was prepared with 2-oxo-1, 2,3, 4-tetrahydroquinoline-6-carboxylic acid (77 mg, 0.4 mmol) (Che » z. Pharm. Bull. 1986, 34, 682) and { [cis-4- (2- phenoxyethyl) cyclohexyl] methyl} amine hydrochloride (108 mg, 0.4 mmol) by a procedure similar to that in Example 8 as a yellow solid (36 mg, 2%).

'H NMR (DMSO-d6) 8 : 10.28 (brs, 1H), 8. 29-8. 24 (m, 1H), 7.69-7. 63 (m, 2H), 7.30-7. 24 (m, 2H), 6.94-6. 85 (m, 4H), 4.01-3. 97 (m, 2H), 3.23-3. 18 (m, 2H), 2.94-2. 88 (m, 2H), 2.47- 2.45 (m, 2H), 1. 78-1. 66 (m, 4H), 1.52-1. 36 (m, 8H) ppm.

MS (ESI): 407.03 (M+H) +, 405.11 (M-H)- Anal. Calcd. for C25H3oN203-0. 6H20 : C, 71.95. ; H, 7.54 ; N, 6. 71. Found: C, 71.84 ; H, 7.47 ; N; 6.49 Example 99 3-Methl-2-oxo-N f fcis-4- (2-phenoxyethyl) cyclohexyllmethvl}-2, 3-dihydro-IH<BR> benzimidazole-5-carboxamide This compound was prepared with 3-methyl-2-oxo-2, 3-dihydro-lH-benzimidazole-5- carboxylic acid and {[cis-4-(2-phenoxyethyl) cyclohexyl] methyl} amine hydrochloride by a procedure similar to that in Example 8.

'H NMR (CDCl3) 8 : 9.32 (br, 1H), 7. 58-7. 54 (m, 1H), 7.45-7. 39 (m, 1H), 7.33-7. 24 (m, 2H), 7.09 (d, J= 8.2 Hz, 1H), 6.98-6. 86 (m, 3H), 6. 18-6. 08 (m, 1H), 4.04-3. 94 (m, 2H), 3.53- 3.40 (m, 5H), 1.94-1. 38 (m, 12H) ppm.

MS (ESI) : 407.99 (M+H) +, 406.07 (M-H)- Example 100 N-({cis-4-[(2-Fluorophenoxy)methyl]cyclohexyl}methyl)-1H-pyr azole-4-carboxamide This compound was prepared with lH-pyrazole-4-carboxylic acid (56 mg, 0.5 mmol) and ({cis-4-[(2-fluorophenoxy) methyl] cyclohexyl} methyl) amine hydrochloride (137 mg, 0.5 mmol) by a procedure similar to that in Example 8 as a white solid (90 mg, 55%).

IH NMR (DMSO-d6) 6 : 13.08 (brs, 1H), 8.11-7. 97 (m, 3H), 7.23-7. 09 (m, 3H), 6.95-6. 89 (m, 1H), 3.96 (d, 2H, J= 5.4 Hz), 3.20-3. 15 (m, 2H), 1. 93-1.48 (m, 10H) ppm.

MS (ESI) : 332.14 (M+H) +, 330.16 (M-H)- Anal. Calcd. for C18H22N302F : C, 65.24 ; H, 6.69 ; N, 12.68. Found: C, 65.19 ; H, 6.54 ; N; 12.64 m. p.: 139. 8°C Example 101 2-Oxo-N- [cis-4-(2-phenoxyethyl)cyclohexyl]methyl}-2,3-dihydro-1,3-be nzothiazole-6- carboxamide This compound was prepared with 2-oxo-2, 3-dihydro-1, 3-benzothiazole-6-carboxylic acid (20 mg, 0.1 mmol) (Chem. Pharm. Bull. 1988, 36, 2253) and { [cis-4- (2- phenoxyethyl) cyclohexyl] methyl} amine hydrochloride (30 mg, 0.1 mmol) by a procedure similar to that in Example 8 as a white solid (28 mg, 67%).

1H NMR (DMSO-d6) 8 : 8. 40-8. 36 (m, 1H), 8. 05-8. 04 (m, 1H), 7.80-7. 76 (m, 1H), 7. 30-7. 24 (m, 2H), 7.17-7. 14 (m, 1H), 6.94-6. 88 (m, 3H), 4.01-3. 96 (m, 2H), 3.25-3. 20 (m, 2H), 1.72- 1.37 (m, 12H) ppm. (NH was not observed.] MS (ESI) : 411.04 (M+H) +, 409.10 (M-H)- Anal. Calcd. for C23H26N203S : C, 67.29. ; H, 6. 38 ; N, 6.82. Found: C, 67.27 ; H, 6.42 ; N; 6.81 m. p.: 159. 9°C, 175. 7°C Example 102 3-Amino-N [cis-4-(2-phenoxvethel) cyclohexvllmethyl T-lH-pyrazole-4-carboxamide This compound was prepared with 3-amino-lH-pyrazole-4-carboxylic acid (64 mg, 0.5 mmol) and { [cis-4- (2-phenoxyethyl) cyclohexyl] methyl} amine hydrochloride (135 mg, 0.5 mmol) by a procedure similar to that in Example 8 as a white solid (81 mg, 47%).

IH NMR (DMSO-d6) b : 11.82-11. 69 (m, 1H), 7.96-7. 65 (m, 2H), 7.30-7. 25 (m, 2H), 6.93- 6. 88 (m, 3H), 5.92-5. 80 (m, 1H), 5.34-5. 22 (m, 1H), 4.00-3. 96 (m, 2H), 3.15-3. 10 (m, 2H), 1.75-1. 62 (m, 4H), 1.52-1. 32 (m, 8H) ppm.

MS (ESI) : 343.17 (M+H) +, 341.17 (M-H)- Anal. Calcd. for Cl9H26N402-0. 2H20: C, 65.95. ; H, 7.69 ; N, 16.19. Found: C, 65. 87 ; H, 7.82 ; N; 16.01. m. p.: 129. 3°C Example 103 N-{[cis-4-(2-Phenoxyethyl)cyclohexyl]methyl}-1H-indazole-5-c arboxamide This compound was prepared with IH-indazole-5-carboxylic acid (65 mg, 0.4 mmol) (Helv. Chim. Acta. 1976, 59, 2618) and {Ecis-4-(2-phenoxyethyl) cyclohexyl] methyl} amine hydrochloride (108 mg, 0.4 mmol) by a procedure similar to that in Example 8 as a white solid (37 mg, 25%).

'H NMR (DMSO-d6) 6 : 13.25 (brs, IH), 8.46-8. 41 (m, 1H), 8.32 (s, 1H), 8.20 (s, 1H), 7.86- 7.83 (m, 1H), 7.58-7. 54 (m, 1H), 7.30-7. 25 (m, 2H), 6. 94-6. 88 (m, 3H), 4.01-3. 97 (m, 2H), 3.28-3. 23 (m, 2H), 1.84-1. 63 (m, 4H), 1. 54-1. 35 (m, 8H) ppm.

MS (ESI): 378.12 (M+H) +, 376.16 (M-H)- Anal. Calcd. for C23H27N302 : C, 73.18. ; H, 7.21 ; N, 11.13. Found: C, 72.80 ; H, 7. 18 ; N; 11.08. m. p.: 144. 5°C Example 104 N-ff[cis-4- (2-Phenoyethyl) cyclohexvllmethyl -1H-1, 2, 3-triazole-4-carboxamide This compound was prepared with IH-1, 2, 3-triazole-4-carboxylic acid (45 mg, 0.4 mmol) (J. Amer. Chem. Soc. 1954, 76, 4931) and {[cis-4-(2- phenoxyethyl) cyclohexyl] methyl} amine hydrochloride (108 mg, 0.4 mmol) by a procedure similar to that in Example 8 as a white solid (24 mg, 19%).

'H NMR (DMSO-d6) 6 : 8. 45-8.30 (m, 2H), 7.30-7. 23 (m, 2H), 6.94-6. 88 (m, 3H), 4.01-3. 96 (m, 2H), 3.25-3. 20 (m, 2H), 1.71-1. 42 (m, 12H) ppm. [NH proton was not observed.] MS (ESI) : 329.10 (M+H) +, 327.12 (M-H)- Anal. Calcd. for C1gH24N4°2 : C, 65.83. ; H, 7.37 ; N, 17.06. Found: C, 65.45 ; H, 7.08 ; N; 17.10. m. p.: 141. 2°C Example 105 N- « cis-4-f (Pvridin-2-yloxy) methvllcvclohexyl} methyl)-1H-pyrazole-4-carboxamide This compound was prepared with lH-pyrazole-4-carboxylic acid and ({cis-4-[(pyridin- 2-yloxy) methyl] cyclohexyl} methyl) amine by a procedure similar to that in Example 8.

'H NMR (CDC13) 8 : 13.07 (br, 1H), 8.21-7. 84 (m, 4H), 7.69 (ddd, J = 8.4, 7.0, 2.1 Hz, 1H), 6.95 (m, 1H), 6. 80 (d, J=8. 2 Hz, 1H), 4. 18 (d, J= 7.1 Hz, 2H), 3.17 (m, 2H), 1.91 (br, 1H), 1.73 (br, 1H), 1.60-1. 31 (m, 8H) ppm.

MS (ESI) : 315.09 (M+H) +, 313.10 (M-H)- IR (KBr) Vmax : 3358, 2849,1631, 1475,1435, 1246,1022, 777 cm~ Example 106 N-( (cis-4-f (3-Fluorophenoxy) methyllcyclohexyl) methyl)-lH-pyrazole-4-carboxamide This compound was prepared with 1H-pyrazole-4-carboxylic acid and (I [cis-4- (3- fluorophenoxymethyl) cyclohexyl] methyl} amine hydrochloride by a procedure similar to that in Example 8.

'H NMR (DMSO-d6) 6 : 13.08 (brs, 1H), 8.00-7. 97 (m, 3H), 7.33-7. 26 (m, 1 H), 6.85-6. 71 (m, 3H), 3.90 (d, J = 6. 8 Hz, 2H), 3.16 (t, J = 6. 6 Hz, 2H), 1.96-1. 85 (m, 1H), 1.79-1. 67 (m, 1H), 1.58-1. 32 (m, 8H) ppm.

MS (ESI): 332.12 (M+H) +, 338.15 (M-H)- IR (KBr) Vmax : 3348,2920, 1625,1577, 1490,1284, 1134,1041 cm~ Example 107 N-f fcis-4- (3-Phenoxypropvl) cyclohexyllmethvl}-lH-pvrazole-4-carboxamide This compound was prepared with lH-pyrazole-4-carboxylic acid and { [cis-4- (3- phenoxypropyl) cyclohexyl] methyl} amine by a procedure similar to that in Example 8.

'H NMR (DMSO-d6) 8 : 13.09 (s, 1H), 8.15-7. 90 (m, 3H), 7. 33-7. 22 (m, 2H), 6.96-6. 87 (m, 3H), 3.94 (t, J = 6.4 Hz, 2H), 3.16 (t, J = 6.4 Hz, 2H), 1.80-1. 63 (m, 4H), 1.53-1. 25 (m, 10H) ppm.

MS (ESI) : 342.09 (M+H) +, 340.12 (M-H)- IR (KBr) vmax : 3310,2924, 1626,1604, 1566,1539, 1499,1246, 752,691 cm~ Example 108 3, 5-Difluoro-4-h droxy-N-f fcis-4- (phenoxymethyl) cyclohexyllmethyl) benzamide This compound was prepared with 3,5-difluoro-4-hydroxybenzoic acid (87 mg, 0.5 mmol) and { [cis-4-(phenoxymethyl) cyclohexyl] methyl} amine hydrochloride (128 mg, 0.5 mmol) by a procedure similar to that in Example 8 as colorless amorphous (49 mg, 26%).

1H NMR (CDC13) 8 : 7.37-7. 25 (m, 5H), 6.96-6. 88 (m, 3H), 6.00 (brs, 1H), 3.88-3. 85 (m, 2H), 3. 44-3. 39 (m, 2H), 2.03-1. 47 (m, 10H) ppm.

MS (ESI) : 376.05 (M+H) +, 374.06 (M-H)- Anal. Calcd. for C2lH23NO3F2-0. 2H20: C, 66.55 ; H, 6.22 ; N, 3.70. Found: C, 66.34 ; H, 6.20 ; N; 3.65 Example 109 N-({cis-4-[(4-Fluorophenoxy)methyl]cclohexyl}methyl)-1H-pyra zole-4-carboxamide This compound was prepared with lH-pyrazole-4-carboxylic acid (22 mg, 0.2 mmol) and ({cis-4-[(4-fluorophenoxy) methyl] cyclohexyl} methyl) amine hydrochloride (55 mg, 0.2 mmol) by a procedure similar to that in Example 8 as a white solid (35 mg, 54%).

'H NMR (DMSO-d6) 6 : 13.08 (brs, 1H), 8.14 (brs, 1H), 8.00-7. 96 (m, 1H), 7. 88 (brs, 1H), 7.13-7. 07 (m, 2H), 6.98-6. 93 (m, 2H), 3. 87-3. 84 (m, 2H), 3.20-3. 15 (m, 2H), 1.88-1. 40 (m, 10H) ppm.

MS (ESI): 332.10 (M+H) +, 330.10 (M-H)- Anal. Calcd. for CgH22N302F : C, 65.24 ; H, 6.69 ; N, 12.68. Found: C, 65.05 ; H, 6.65 ; N; 12.67 m. p.: 147. 1°C Example 110 N-f {[cis-4-(Benzyloxy)cyclohexyl]methyl}-1H-pyrazole-4-carboxam ide This compound was prepared with 1H-pyrazole-4-carboxylic acid and { [cis-4- (benzyloxy) cyclohexyl] methyl} amine by a procedure similar to that in Example 8.

'H NMR (DMSO-d6) S : 13.08 (s, 1H), 8.23-7. 80 (m, 3H), 7.40-7. 20 (m, 5H), 4.45 (s, 2H), 3.63-3. 53 (m, 1H), 3.08 (t, J = 6.4 Hz, 2H), 1.90-1. 77 (m, 2H), 1.65-1. 20 (m, 7H) ppm.

MS (ESI) : 314.08 (M+H) +, 312.07 (M-H)- IR (KBr) v, : 3335,3126, 2928, 1630,1580, 1537,1246, 1065,735, 696 cm~ Example 111 N-(( cis-4- (3-Methoxvphenoxy methvllcyclohexyl methyl)-lH-pyrazole-4-carboxamide This compound was prepared with lH-pyrazole-4-carboxylic acid and ({cis-4-[(3- methoxyphenoxy) methyl] cyclohexyl} methyl) amine by a procedure similar to that in Example 8.

'H NMR (DMSO-d6) 6 : 13.07 (brs, 1H), 8.20-8. 10 (m, 1H), 8.03-7. 95 (m, 1H), 7.92-7. 84 (m, 1H), 7.16 (m, 1H), 6.56-6. 46 (m, 3H), 3.86 (d, J= 6. 8 Hz, 2H), 3.73 (s, 3H), 3.18 (t, J= 6.8 Hz, 2H), 1.97-1. 82 (m, 1H), 1.80-1. 65 (m, 1H), 1.59-1. 30 (m, 8H) ppm.

MS (ESI) : 344.18 (M+H) +, 342.25 (M-H)- Example 112 N-({2R,5R)-5-[(4-Fluorophenoxy)methyl]tetrahydro-2H-pyran-2- yl}methyl)-1H-pyrazole- 4-carboxamide This compound was prepared with 1H-pyrazole-4-carboxylic acid by a procedure similar to that in Example 75.

IH NMR (DMSO) S : 13.08 (bs, 1H), 8.24-7. 83 (m, 3H), 7.17-6. 92 (m, 4H), 4.17-3. 87 (m, 3H), 3.58-3. 11 (m, 4H), 2.03-1. 26 (m, 5H) ppm.

Example 113 and 114 4 stereoisomers were prepared with 1H-pyrazole-4-carboxylic acid and ( {5- [2- (4- fluorophenoxy) ethyl] tetrahydro-2H-pyran-2-yl} methyl) amine by a procedure similar to that in Example 8.

4 stereoisomers were separated by Chiral column (Chiralpak AD-H, 20 mm I. D. x 250 mm (No. ADHOCJ-DE003), DAICEL) using n-Hexane: 2-Propanol: Et2NH = 85 : 15: 0.1 as an eluent (10 mL/min).

Example 113 N ( ( (2R, SS)-5-r2- (4-Fluorophenoxv) ethylltetrahydro-2H-pvran-2-vlmethvl)-lH-pyrazole- 4-carboxamide Retention time 29min-32min 'H NMR (DMSO-d) 8 : 13.28 (br, 1H), 8.15-7. 93 (m, 3H), 7.10 (t, J = 8.8 Hz, 2H), 6.94 (dd, J = 9. 0,4. 6 Hz, 2H), 3.99 (t, J = 6. 3 Hz, 2H), 3.73 (m, 1H), 3.55-3. 10 (m, 4H), 1.96-1. 65 (m, 5H), 1.50-1. 35 (m, 2H) ppm.

MS (ESI) : 348.16 (M+H) +, 346.17 (M-H)- Example 114 N-({(2S,5R)-5-[2-(4-fluorophenoxy)ethyl]tetrahydro-2H-pyran- 2-yl}methyl)-1H-pyrazole-4- carboxamide Retention time 39min-43min.

'H NMR (DMSO-d) 6 : 13.09 (br, 1H), 8.18-7. 96 (m, 3H), 7.10 (t, J = 8.9 Hz, 2H), 6.94 (dd, J = 9.2, 4.4 Hz, 2H), 3.99 (t, J = 6.4 Hz, 2H), 3.73 (m, 1H), 3.54-3. 13 (m, 4H), 1.96-1. 61 (m, 5H), 1.50-1. 35 (m, 2H) ppm.

MS (ESI): 348. 09 (M+H) +, 346. 11 (M-H)- Example 115 N-f {[cis-4-(4-Methoxybenzyl)cyclohexyl]methyl}-1H-pyrazole-4-ca rboxyamide This compound was prepared with lH-pyrazole-4-carboxylic acid and { [cis-4- (4- methoxybenzyl) cyclohexylmethyl} amine by a procedure similar to that in Example 8.

'H NMR (DMSO) 6 : 13.07 (bs, 1H), 8.19-7. 83 (m, 3H), 7.07 (d, J= 8.6 Hz, 2H), 6.82 (d, J = 8. 6 Hz, 2H), 3.71 (s, 3H), 3.23-3. 11 (m, 2H), 2.50-2. 43 (m, 2H), 1.78-1. 20 (m, 9H) ppm.

EXAMPLES 116 AND 116 (2) N-{[(1R,3S)-3-(2-Phenylethoxy)cyclohexyl]methyl]-1H-pyrazole -4-carboxamide and N- {[(1S,3R)-3-(2-Phenylethoxy)cyclohexyl]methyl}-1H-pyrazole-4 -carboxamide N-{[cis-3-(2-phenylethoxy)cyclohexyl]methyl}-1H-pyrazole-4-c arboxamide (0. 11g, 0.34 mmol) was prepared with lH-pyrazole-4-carboxylic acid and { [(cis-3-(2- Phenylethoxy) cyclohexyl) methyl] amine by a procedure similar to that in Example 8, and separated by chiral column (Chiralcel OJ-H, 20 mm I. D. x 250 mm (No. OJHOCJ-DH004), DAICEL) using n-Hexane/EtOH/Et2NH = 93/7/0. 1 as an eluent (Flow rate: 10 mL/min) to give the titled compounds.

Example 116: First peak: (32 mg) retention time 39.8 min, >99% ee.

'H NMR (DMSO-d6) 8 : 13.08 (s, 1H), 8. 15-7.95 (m, 3H), 7.32-7. 12 (m, SH), 3.61 (t, J = 7.1 Hz, 2H), 3. 28-2. 96 (m, 3H), 2.76 (t, J= 7.1 Hz, 2H), 2.08-1. 90 (m, 2H), 1. 78-1. 40 (m, 3H), 1.27-0. 70 (m, 4H) ppm.

MS (ESI) : 328.15 (M+H) +, 326.23 (M-H)- Example 116 (2): Second peak: (27 mg) retention time 45.3 min, >99% ee IH NMR data was identical with that of example 116.

MS (ESI) : 328.15 (M+H) +, 326.23 (M-H)- Example 117 3-Amino-N-f (cis-4-benzvlcyclohexvl) methvll-lH-pyrazole-4-carboxamide This compound was prepared with 3-amino-lH-pyrazole-4-carboxylic acid (38 mg, 0.3 mmol) and [(cis-4-benzylcyclohexyl) methyl] amine (61 mg, 0.3 mmol) by a procedure similar to that in Example 8 as a white solid (8.8 mg, 9%).

'H NMR (DMSO-d6) 8 : 11. 80-11. 69 (m, 1H), 7.93-7. 64 (m, 2H), 7.30-7. 15 (m, 5H), 5.86- 5.27 (m, 2H), 3.16-3. 12 (m, 2H), 2.56-2. 53 (m, 2H), 1.67-1. 40 (m, 10H) ppm.

MS (ESI) : 313.23 (M+H) +, 311.13 (M-H)- Example 118 N-f (cis-4-Benzvlcyclohexyl) methyll-2-oxo-1, 2, 3, 4-tetrahydroquinoline-6-carboxamide This compound was prepared with 2-oxo-1, 2,3, 4-tetrahydroquinoline-6-carboxylic acid (57 mg, 0.3 mmol) and [(cis-4-benzylcyclohexyl)methyl] amine (61 mg, 0.3 mmol) by a procedure similar to that in Example 8 as a white solid (35 mg, 31%).

'H NMR (DMSO-d6) 8 : 10.27 (brs, 1H), 8. 28-8.23 (m, 1H), 7.69-7. 63 (m, 2H), 7.30-7. 15 (m, 5H), 6.88-6. 85 (m, 1H), 3.25-3. 20 (m, 2H), 2.94-2. 88 (m, 2H), 2.56-2. 45 (m, 4H), 1.73- 1.29 (m, 10H) ppm.

MS (ESI) : 377.21 (M+H) +, 375.20 (M-H)- Anal. Calcd. for CzgNzOz-0. 1H20 : C, 76.20 ; H, 7. 51 ; N, 7. 41. Found: C, 76.11 ; H, 7.57 ; N; 7.31 m. p. 188. 5°C Example 119 N-({(2R,5R)-5-[(3,4-Difluorophenoxy)methyl]tetrahydro-2H-pyr an-2-yl}methyl)-1H- pyrazole-4-carboxamide To a solution of tert-butyl { [(5S)-5-(hydroxymethyl)tetrahydro-2H-pyran-2- yl] methyl} carbamate (300 mg, 1.22 mmol) and 3,4-difluorophenol (397.7 mg, 3.06 mmol) in THF (4.9 mL), were added PPh3 (737.7 mg, 2.81 mmol) and DIAD (0.56 mL, 2.32 mmol) at 0 °C. The mixture was irradiated by microwave at 180 °C for 5 min. Then the mixture was cooled to room temprature and was diluted AcOEt. The oganic layer was washed with 2N NaOH aq. and brine. The organic layer was dried over Na2SO4, was filtered and evaporated. The crude product was purified by silica gel column chromatography (hexane: AcOEt = 50: 1-20: 1) to give tert-butyl ({(2R, SR)-5-[(3, 4- difluorophenoxy) methyl] tetrahydro-2H-pyran-2-yl} methyl) carbamate (55.5 mg, 0.155 mmol) This was dissolved in HCl-MeOH (1 mL) and the mixture was stirred at 40 °C for 2hr. The mixture was evaporated to give the crude amine. The amine was dissolved in DMF (2 mL) and were added lH-pyrazole-4-carboxylic acid (17.4 mg, 0.155 mmol), Et3N (0.064 mL, 0.466 mmol), HOBt (28.5 mg, 0.186 mmol) and WSC (35.6 mg, 0.186 mmol) at 0 °C.

The mixture was stirred at room temperature overnight. 2N NaOH aq was added to the mixture and the mixture was stirred at room temperature for 1 hr. The mixture was extracted with AcOEt and the organic layer was washed with brine. The organic layer was dried over Na2S04, was filtered and evaporated. The crude product was purified by silica gel column chromatography (CH2CI2 : MeOH = 20 : 1) to give the titled compound.

'H NMR (DMSO-d) 6 : 13.08 (br, 1H), 8.17-7. 92 (m, 3H), 7.35-7. 25 (m, 1H), 7.13-7. 05 (m, 1H), 6. 84-6. 75 (m, 1H), 4.14 (t, J = 9.1 Hz, 1H), 4.03-3. 87 (m, 2H), 3.58-3. 11 (m, 4H), 1.94 (br, 1H), 1. 88-1. 64 (m, 2H), 1.53-1. 29 (m, 2H) ppm.

MS (ESI): 352.20 (M+H) +, 350.15 (M-H)- EXAMPLE120 AND EXAMPLE121 To a suspension of LiAlH4 (119.7 mg, 3., 15 mmol) in THF (10 mL) the solution of 2- (azidomethyl)-5- [ (4-chlorophenoxy) methyl] tetrahydro-2H-pyran (444.3 mg, 1. 58 mmol) in THF (6 mL) was added at 0 °C. Then the mixture was stirred at 0 °C for 1.25 hr. The reaction was quenched by Na2S04-10H20 (1. 6 g, 4.97 mmol) and KF (200 mg, 3.44 mmol).

The mixture was stirred at room temperature for lhr. The mixture was filtered through a pad of celite and the filtrate was evaporated to give the crude compound.

To a solution of the crude compound in DMF (5 mL), were added lH-pyrazole-4-carboxylic acid (177.1 mg, 1.58 mmol), HOBt (290.4 mg, 1.90 mmol) and WSC (363.5 mg, 1.90 mmol) at 0 °C. The mixture was stirred at room temperature overnight. 2N NaOH aq was added to the mixture and the mixture was stirred at room temperature for 1 hr. The mixture was extracted with AcOEt and the organic layer was washed with brine. The organic layer was dried over Na2S04, was filtered and evaporated. The crude product was purified by silica gel column chromatography (CHzCI2 : MeOH = 20 : 1) to give the mixture of 4 stereoisomers. 4 stereoisomers were separated by Chiral column (Chiralcel OJ-H, 20 mm I. D. x 250 mm (No. OJHOCJ-DH004), DAICEL) using n-Hexane: EtOH: Et2NH = 88: 12: 0.1 as an eluent (18. 9 mLJmin).

Example 120 N-({(2R,5R)-5-[(4-Chlorophenoxy)methyl]tetrahydro-2H-pyran-2 -yl}methyl)-1H-pyrazole- 4-carboxamide Retention time 12 min-20 min (13 min) lH NMR (DMSO-d) 8 : 13. 10 (br, 1H), 8.23-7. 83 (m, 3H), 7.33 (d, J = 9.0 Hz, 2H), 6.99 (d, J = 8.8 Hz, 2H), 4.14 (t, J = 9.0 Hz, 1H), 4.05-3. 86 (m, 2H), 3. 58-3. 12 (m, 4H), 1.95 (br, 1H), 1.89-1. 66 (m, 2H), 1.53-1. 20 (m, 2H) ppm.

MS (ESI) : 350.05 (M+H) +, 348. 06 (M-H)- Example 121 N-({(2S,5S)-5-[(4-Chlorophenoxy)methyl]tetrahydro-2H-pyran-2 -yl}methyl)-1H-pyrazole- 4-carboxamide Retetntion time 20 min-24 min (22 min) 'H NMR (DMSO-d) b : 13.09 (br, 1H), 8. 20-7. 85 (m, 3H), 7.32-7. 28 (m, 2H), 7.04-6. 94 (m, 2H), 4.14 (t, J= 8.7 Hz, 1H), 4.05-3. 86 (m, 2H), 3.60-3. 10 (m, 4H), 1.95 (br, 1H), 1. 86-1. 64 (m, 2H), 1.53-1. 20 (m, 2H) ppm.

MS (ESI): 350.04 (M+H)+, 348.06 (M-H)- Example 122 N (cis-4-BenzylcyclohexYl) methvl1-2-hvdroxyquinoline-6-carboxamide This compound was prepared with 2-hydroxyquinoline-6-carboxylic acid (38 mg, 0.2 mmol) and [(cis-4-benzylcyclohexyl) methyl] amine (53 mg, 0.2 mmol) by a procedure similar to that in Example 8 as a white solid (26 mg, 34%).

IH NMR (DMSO-d6) 5 : 11.93 (brs, 1H), 8. 46-8. 42 (m, 1H), 8.18-8. 17 (m, 1H), 7.97-7. 94 (m, 2H), 7.33-7. 25 (m, 3H), 7. 18-7.16 (m, 3H), 6.57-6. 53 (m, 1H), 3.29-3. 24 (m, 2H), 2. 58- 2.55 (m, 2H), 1.83-1. 64 (m, 2H), 1.44-1. 30 (m, 8H) ppm.

MS (ESI): 375.06 (M+H) +, 373.08 (M-H)- Anal. Calcd. for C24H26N202-0. 4H20 : C, 75.52 ; H, 7.08 ; N, 7.34. Found: C, 75. 18 ; H, 6. 94 ; N; 7.09 m. p.: 236. 7°C Example 123 N-({(2R,5R)-5-[(4-Methylphenoxy)methyl]tetrahydro-2H-pyran-2 -yl}methyl)-1H-pyrazole- 4-carboxamide This compound was prepared with p-cresol by a procedure similar to that in Example 119.

Cis and trans isomers were separated by Chiral column (Chiralcel OJ-H, 20 mm I. D. x 250 mm (No. OJHOCJ-DH004), DAICEL) using 5 min-7 min (5 min) 'H NMR (DMSO-d) 5 : 13.10 (br, 1H), 8.21-7. 86 (m, 3H), 7. 08 (d, J = 8. 1 Hz, 2H), 6.84 (d, J = 8.6 Hz, 2H), 4.10 (t, J = 9.0 Hz, 1H), 3.99-3. 87 (m, 2H), 3.57-3. 12 (m, 4H), 2.23 (s, 3H), 1.98-1. 65 (m, 3H), 1.52-1. 28 (m, 2H) ppm.

MS (ESI): 330.10 (M+H) , 28.12 (M-H)- Example 124 3-Amino-N-(T (2R, 5R)-5-F (4-fluorophenoxy) methylltetrahydro-2H-pvran-2-yl lmethyl)-1H- pyrazole-4-carboxamide 3-Amino-N-({ (2R*, SR*)-5-[(4-fluorophenoxy) methylatetrahydro-2H-pyran-2- yl} methyl)-lH-pyrazole-4-carboxamide was prepared with 3-amino-1H-pyrazole-4- carboxylic acid (127 mg, 1.0 mmol) and ( { (ZR* SR*)-5- [ (4- fluorophenoxy) methyl] tetrahydro-2H-pyran-2-yl} methyl) amine (239 mg, 1.0 mmol) by a procedure similar to that in Example 8, and separated by Chiral column (Chiralpak OJ-H, 20 mm I. D. x 250 mm (No. OJHOCJ-DH004), DAICEL) using n-Hexane/Ethanol/Et2NH 85/15/0.1 as an eluent (10 mL/min) to afford the titled compound (50 mg, 14%).

'H NMR (DMSO-d6) 8 : 11.73 (brs, 1H), 7. 83-7. 67 (m, 2H), 7.14-7. 07 (m, 2H), 6.99-6. 94 (m, 2H), 5.85 (brs, 1H), 4.15-4. 08 (m, 1H), 3.99-3. 90 (m, 2H), 3.55-3. 50 (m, 1H), 3.47-3. 37 (m, 1H), 3.27-3. 09 (m, 2H), 1.94-1. 72 (m, 3H), 1.43-1. 24 (m, 2H) ppm. (1H was not observed.) MS (ESI): 349.15 (M+H) +, 347.14 (M-H)- Anal. Calcd. for C17H2, FN403-0. 2H20 : C, 58.01 ; H, 6.13 ; N, 15.92. Found: C, 58.03 ; H, 6.34 ; N; 15.60 Example 125 3-Amino-N-({(2R,5R)-5-[(4-chlorophenoxy)methyl]tetrahydro-2H -pyran-2-yl}methyl)-1H- pyrazole-4-carboxamide This compound was prepared with 4-chlorophenol and 3-amino-1H-pyrazole-4-carboxylic acid by a procedure similar to that in Example 119. IH NMR (DMSO-d6) 6 : 13.70 (s, 1H), 7.82-7. 65 (m, 2H), 7.30 (d, J = 8.9 Hz, 2H), 6.98 (d, J = 8.9 Hz, 2H), 4.20-3. 85 (m, 3H), 3.60-3. 05 (m, 4H), 2.00-1. 62 (m, 3H), 1.50-1. 05 (m, 2H) ppm. (-NH2 was not observed.) MS (ESI): 365.07 (M+H) +, 363.09 (M-H) +- Example 126 N ((f (2R, 5R)-5-r (4-Chlorophenoxv) methvlltetrahydro-2H-pyran-2-yl} methyl)-3, 5-difluoro- 4-hydroxybenzamide This compound was prepared with 4-chlorophenol and 3, 5-difluoro-4-hydroxybenzoic acid by a procedure similar to that in Example 119.

'H NMR (DMSO-d6) 6 : 8.52-8. 42 (m, 1H), 7.63-7. 46 (m, 2H), 7.30 (d, J = 8.8 Hz, 2H), 6. 98 (d, J = 8. 8 Hz, 2H), 4.18-4. 07 (m, 1H), 4.02-3. 85 (m, 2H), 3.57-3. 20 (m, 4H), 2.00- 1.63 (m, 3H), 1.53-1. 15 (m, 2H) ppm. (-OH was not observed.) MS (ESI) : 412.13 (M+H) +, 410. 13 (M-H) +- Example 127 N-({(2R,5R)-5-[(4-Chlorophenoxy)methyl]tetrahydro-2H-pyran-2 -yl}methyl)-2-oxo- 1, 2, 3, 4-tetrahydroquinoline-6-carboxamide This compound was prepared with 4-chlorophenol and 2-oxo-1,2, 3,4- tetrahydroquinoline-6-carboxylic acid by a procedure similar to that in Example 119 as a white solid.

IH NMR (CDC13) 5 : 8.48 (s, 1H), 7.64 (s, 1H), 7.59-7. 57 (m, 1H), 7.27-7. 20 (m, 2H), 6.85- 6.79 (m, 3H), 6.55-6. 52 (m, 1H), 4.17-4. 11 (m, 2H), 3.99-3. 93 (m, 1H), 3.84-3. 76 (m, 1H), 3.68-3. 63 (m, 1H), 3.61-3. 53 (m, 1H), 3.27-3. 18 (m, 1H), 3.02-2. 96 (m, 2H), 2.69-2. 64 (m, 2H), 2.11-1. 76 (m, 3H), 1.64-1. 40 (m, 1H), 1.36-1. 22 (m, 1H) ppm.

MS (ESI) : 429 (M+H) + Example 128 N-({(2R,5R)-5-[(4-Chlorophenoxy)methyl]tetrahydro-2H-pyran-2 -yl}methyl)-3-methyl-1H- pyrazole-4-carboxami de This compound was prepared with 4-chlorophenol and lH-3-methylpyrazole-4- carboxylic acid by a procedure similar to that in Example 119.

'H NMR (CDC13) 6 : 7. 86 (s, 1H), 7.21 (d, J= 8.8 Hz, 2H), 6.96-6. 87 (m, 1H), 6. 83 (d, J= 8.8 Hz, 2H), 4.18-4. 10 (m, 2H), 3.97-3. 92 (m, 1H), 3.83-3. 67 (m, 3H), 3.21-3. 16 (m, 1H), 2.50 (s, 3H), 2.13-2. 03 (m, 1H) 1. 98-1. 76 (m, 2H), 1.62-1. 39 (m, 1H), 1.33-1. 22 (m, 1H) ppm. (1H was not observed.) MS (ESI): 364 (M+H)+ Example 129 N ( f (2R, 5R)-5-r (4-Chloro-3-fluorophenoxy) methylltetrahydro-2H-pyran-2-ylmethyl)-1H- pyrazole-4-carboxami de This compound was prepared with 4-chloro-3-fluorophenol and lH-pyrazole-4- carboxylic acid by a procedure similar to that in Example 119.

'H NMR (DMSO-d6) 8 : 13.09 (brs, 1H), 8. 12-8. 03 (m, 3H), 7.46 (t, J= 8. 1 Hz, 1H), 7.14- 7.09 (m, 1H), 6.88-6. 84 (m, 1H), 4.18 (t, J= 8.1 Hz, 1H), 4.04-3. 98 (m, 1H), 3.93-3. 89 (m, 1H), 3.55-3. 50 (m, 1H), 3.47-3. 40 (m, 1H), 3.29-3. 13 (m, 2H), 1.96-1. 70 (m, 3H), 1. 50-1. 36 (m, 2H) ppm.

MS (ESI): 368.03 (M+H) +, 366.03 (M-H)- Example 130 3-Amino-N-({ (2R, SR)-5-r (4-ethylphenoxy) methylltetrahydro-2H-pyran-2-yl dmethyl)-lH- pyrazole-4-carboxamide This compound was prepared with 4-ethylphenol and 3-amino-lH-pyrazole-4-carboxylic acid by a procedure similar to that in Example 119.

IH NMR (CDCI3) 5 : 7.55 (s, 1H), 7.11-7. 08 (m, 2H), 6.85-6. 81 (m, 2H), 6. 81-6. 72 (m, 1H), 5.58-5. 31 (m, 2H), 4.17-4. 07 (m, 2H), 3. 98-3. 93 (m, 1H), 3.72-3. 49 (m, 3H), 3.14-3. 05 (m, 1H), 2.57 (d, J = 7.5 Hz, 2H), 2.07-1. 79 (m, 3H), 1.62-1. 35 (m, 2H), 1.19 (t, J = 7.5 Hz, 3H) ppm. (NH was not observed.) MS (ESI) : 359 (M+H) + Example 131 N-({(2R,5R)-5-[(4-Cycloproppylphenoxy)methyl]tetrahydro-2H-p yran-2-yl}methyl)-1H- pyrazole-4-carboxamide This compound was prepared with 4-cyclopropylphenol by a procedure similar to that in Example 119 as colorless oil.

'H NMR (DMSO-d6) 8 : 13. 09 (brs, IH), 8. 10-8. 03 (m, 3H), 7.00-6. 96 (m, 2H), 6. 85-6. 81 (m, 2H), 4.12-4. 06 (m, 1H), 3.97-3. 89 (m, 2H), 3.55-3. 49 (m, 1H), 3. 48-3. 40 (m, IH), 3.28- 3.18 (m, 2H), 1.93-1. 67 (m, 4H), 1.44-1. 34 (m, 2H), 0.90-0. 83 (m, 2H), 0.59-0. 53 (m, 2H) ppm.

MS (ES1) : 356.14 (M+H) +, 354.16 (M-H)- Anal. Calcd. for C2oH2sN303'0. 3H20 : C, 66.57 ; H, 7.15 ; N, 11.65. Found: C, 66.41 ; H, 7.17 ; N; 11. 49 Example 132 3-Amino-N-({(2R,5R)-5-[(4-isopropylphenoxy)methyl]tetrahydro -2H-pyran-2-yl}methyl)- 1H-pyrazole-4-carboxamide This compound was prepared with 4-isopropylphenol and 3-amino-lH-pyrazole-4- carboxylic acid by a procedure similar to that in Example 119 as colorless oil.

'H NMR (DMSO-d6) 8 : 11.76 (brs, 1H), 7.86-7. 71 (m, 2H), 7.14 (d, J = 8.1 Hz, 2H), 6. 87 (d, J= 8.1 Hz, 2H), 4.13-4. 07 (m, 1H), 3.99-3. 89 (m, 2H), 3.55-3. 50 (m, 2H), 3.30-3. 08 (m, 2H), 2.87-2. 77 (m, 1H), 1.93-1. 67 (m, 3H), 1.48-1. 36 (m, 2H), 1.18-1. 15 (m, 6H) ppm.

(NH2 were not observed.) MS (ESID : 373.21 (M+H) +, 371.21 (M-H)- Examplel33 3-Amino-N-({(2R,5R)-5-[(4-methylphenoxy)methyl]tetrahydro-2H -pyran-2-yl}methyl)-1H- pyrazole-4-carboxamide This compound was prepared with p-cresol and 3-amino-1H-pyrazole-4-carboxylic acid by a procedure similar to that in Example 119.

'H NMR (DMSO-d) 8 : 11.73 (br, 1H), 8.20-7. 50 (m, 2H), 7. 08 (d, J= 8.3 Hz, 2H), 6.85 (d, J = 8. 4 Hz, 2H), 6.10-5. 20 (m, 2H), 4.10 (t, J = 8. 9 Hz, 1H), 3.97-3. 89 (m, 2H), 3.53 (dd, J = 11.5, 2.5 Hz, 1H), 3.45-3. 38 (m, 1H), 3. 28-3. 22 (m, 1H), 3. 18-3. 12 (m, 1H), 2.24 (s, 3H), 1.97-1. 91 (m, 1H), 1.87-1. 81 (m, 1H), 1.77-1. 68 (m, 1H), 1.48-1. 43 (m, 1H), 1. 39-1. 31 (m, 1H) ppm.

MS (ESI): 345.23 (M+H) +, 343.22 (M-H) - Examplel34 3-Amino-N ( (2R, SR)-5-f (3-fluoro-4-methylphenoxy) methylltetrahydro-2H-pvran-2- yl}methyl)-1H-pyrazole-4-carboxamide This compound was prepared with 3-fluoro-4-cresol and 3-amino-lH-pyrazole-4- carboxylic acid by a procedure similar to that in Example 119.

'H NMR (DMSO-d) 6 : 11.70 (br, 1H), 8.10-7. 50 (m, 2H), 7.15 (d, J = 8.8 Hz, 1H), 6. 78 (dd, J = 11.9, 2.4 Hz, 1H), 6.71 (dd, J = 8.4, 2.4 Hz, 1H), 6.20-5. 05 (m, 2H), 4.12 (t, J = 9.1 Hz, 1H), 3.96 (dd, J = 9. 4,6. 7 Hz, 1H), 3.90 (d, J = 11. 6 Hz, 1H), 3.51 (dd, J = 11. 6,2. 6 Hz, 1H), 3.44-3. 38 (m, 1H), 3.28-3. 20 (m, 1H), 3.18-3. 10 (m, 1H), 2.14 (s, 3H), 1.97-1. 69 (m, 1H), 1. 84-1. 77 (m, 1H), 1.76-1. 67 (m, 1H), 1.48-1. 42 (m, 1H), 1.40-1. 30 (m, 1H) ppm.

MS (ESI) : 363.18 (M+H) +, 361.13 (M-H)- Examplel35 N- (f (2R, 5R)-5-[ (3-Fluoro-4-methylphenoxy) methyl]tetrahydro-2H-pyran-2-yl}methyl)-1H- pyrazole-4-carboxamide This compound was prepared with 3-fluoro-4-cresol by a procedure similar to that in Example 119. 'H NMR (DMSO-d) 8 : 13.08 (br, 1H), 8. 20-7.85 (m, 3H), 7.15 (t, J = 8. 8 Hz, 1H), 6.78 (dd, J=11. 9,2. 4 Hz, 1H), 6.70 (dd, J=8. 4,2. 4 Hz, 1H), 4.12 (t, J=9.0 Hz, 1H), 3.96 (dd, J= 9.4, 6.7 Hz, 1H), 3.90 (d, J = 11.6 Hz, 1H), 3.52 (dd, J= 11.6, 2.6 Hz, 1H), 3.47-3. 41 (m, 1H), 3.37-3. 25 (m, 1H), 3.22-3. 15 (m, 1H), 2.14 (s, 3H), 1.96-1. 90 (m, 1H), 1.84-1. 78 (m, 1H), 1.76-1. 68 (m, 1H), 1.48-1. 43 (m, 1H), 1. 42-1. 33 (m, 1H) ppm.

MS (ESI): 348. 21 (M+H) +, 346.15 (M-H)- Example 136 3-Amino-N-({(2R,5R)-5-[(2,3-dihydro-1H-inden-5-yloxy)methyl] tetrahydro-2H-pyran-2- yl}meth l-pyrazole-4-carboxamide This compound was prepared with indan-5-ol and 3-amino-lH-pyrazole-4-carboxylic acid by a procedure similar to that in Example 119.

1H NMR (DMSO-d) # : 11.81 (br, 1H), 8.20-7. 50 (m, 2H), 7.08 (d, J= 8. 1 Hz, 1H), 6.84- 6.80 (m, 1H), 6.69 (dd, J= 8.1, 2.2 Hz, 1H), 6.20-5. 00 (m, 2H), 4.09 (t, J= 8.9 Hz, 1H), 3.97-3. 86 (m, 2H), 3.51 (dd, J= 11.6, 2. 6 Hz, 1H), 3.44-3. 37 (m, 1H), 3.27-3. 19 (m, 1H), 3.18-3. 10 (m, 1H), 2.81 (t, J = 7.4 Hz, 2H), 2.76 (t, J = 7.3 Hz, 2H), 2.03-1. 96 (m, 2H), 1.95-1. 89 (m, 1H), 1.85-1. 78 (m, 1H), 1. 76-1. 64 (m, 1H), 1.48-1. 41 (m, 1H), 1.39-1. 29 (m, 1H) ppm.

MS (ESI) : 371.12 (M+H) +, 369.14 (M-H)- Example 137 N-({(2R,5R)-5-[(2,3-Dihydro-1H-inden-5-yloxy)methyl]tetrahyd ro-2H-pyran-2-yl}methyl)- 1H-pyrazole-4-carboxamide This compound was prepared with indan-5-ol by a procedure similar to that in Example 119.

1H NMR (DMSO-d) 8 : 13.08 (br, 1H), 8.20-7. 85 (m, 3H), 7. 08 (d, J= 8.2 Hz, 1H), 6.82 (s, 1H), 6.89 (dd, J = 8. 2,2. 2 Hz, 1H), 4.09 (t, J = 8.9 Hz, 1H), 3.97-3. 88 (m, 2H), 3.52 (dd, J = 11.6, 2.6 Hz, 1H), 3.47-3. 40 (m, 1H), 3.38-3. 24 (m, 1H), 3.23-3. 14 (m, 1H), 2.81 (t, J = 7.4 Hz, 2H), 2.76 (t, J= 7.3 Hz, 2H), 2.04-1. 90 (m, 3H), 1. 85-1. 78 (m, 1H), 1.75-1. 65 (m, 1H), 1.49-1. 42 (m, 1H), 1.40-1. 31 (m, 1H) ppm.

MS (ESI) : 356.21 (M+H) +, 354.12 (M-H)- Preparation 1 cis-Methvl-4-r (benzylamino) carbonyl]cyclohexanecarboxylate A mixture of cis-4- (methoxycarbonyl) cyclohexanecarboxylic acid (35 g, 0.19 mol) (J.

Am. Chem. Soc. 1956, 78, 4000-4002. ), benzyl amine (22 g, 0.21 mol), EDCI (40 g, 0. 21 mol) and HOBt-H2O (5.7 g, 37 mmol) in DMF (380 mL) was stirred at room temperature for 16 hours. The mixture was concentrated under reduced pressure and diluted with AcOEt.

The organic layer was washed with 2 N aq. HCI, sat. aq. NaHC03 and water, dried over MgS04, and concentrated in vacuum to give the titled compound. (51 g) 'H NMR (CDC13) 8 : 7. 38-7. 22 (m, 5H), 5.78 (br, 1H), 4.44 (d, J = 5. 8 Hz, 2H), 3.69 (s, 3H), 2.63-2. 54 (m, 1H), 2.30-2. 05 (m, 3H), 1.80-1. 50 (m, 6H) ppm.

Preparation 2 {cis-4-[(Benzylamino)methyl]cyclohexyl}methanol A solution of cis-Methyl 4-[(benzylamino) carbonyl] cyclohexanecarboxylate (51 g, 0.19 mol) in THF (200 mL) was added dropwise to a suspension of LiAlH4 (21 g, 0.56 mol) in THF (1.0 L) at 0 °C and the mixture was refluxed for 16 hours. The reaction mixture was added dropwise to a suspension of NaxSC-lOHzO (excess) in CH2CI2 at 0 °C and the mixture was stirred at room temperature for 3 hours. The white suspension was filtered and the filtrate was concentrated in vacuum. The residue was dissolved with CH2CI2 and filtered through cotton. The filtrate was evaporated to give the titled compound (40 g, 0.17 mol).

1H NMR (CDCl3) 8 : 7.35-7. 20 (m, 5H), 3.78 (s, 2H), 3.52 (d, J = 6.9 Hz, 2H), 2.55 (d, J = 7.1 Hz, 2H), 1.90-1. 30 (m, 10H) ppm. (OH and NH were not observed.) Preparation 3 rcis-4- (Aminomethyl) cyclohexyllmethanol A mixture of {cis-4-[(benzylamino) methyl] cyclohexyl} methanol (35 g, 0.15 mol) and 20% w/w Pd (OH) 2-C (3.0 g) in MeOH (300 ml) was hydrogenated at 4 atm for 10 h. The mixture was filtered through a pad of celite and the filtrate was evaporated to give the titled compound (22 g).

1H NMR (CDC13) 8 : 3.52 (d, J = 6.9 Hz, 2H), 2.61 (d, J = 6.1 Hz, 2H), 1. 80-1. 30 (m, 10H) ppm. (OH and NH2 were not observed.) Preparation 4 4-(Benzyloxy)-N-{[cis-4-(hydroxymethyl)cyclohexyl]methyl}ben zamide To a mixture of [cis-4- (aminomethyl) cyclohexyl] methanol (2.8 g, 20 mmol), triethylamine (3.3 mL, 24 mmol) and DMAP (0.24 g, 2.0 mmol) in CH2Cl2, TBSCI (3.3 g, 22 mmol) was added at 0 °C and the mixture was stirred at room temperature for 16 hours.

To the mixture, sat. aq. NaHC03 was added and the whole was extracted with CH2C12. The extract was dried over MgS04 and evaporated to afford { [cis-4- (f [tert- butyl (dimethyl) silyl] oxy} methyl) cyclohexyl] methyl} amine. A mixture of {[cis-4-({[tert- butyl(dimethyl)silyl]oxy}methyl)cyclohexyl]methyl}amine, 4-(benzyloxy) benzoic acid (4.6 g, 20 mmol), EDCI (4.2 g, 22 mmol) and HOBt-H2O (3. 4 g, 22 mmol) in DMF (40 mL) was stirred at room temperature for 16 hours. The mixture was diluted with AcOEt and washed with sat. aq. NaHC03 and water, dried over MgSO4, and evaporated to afford 4- (benzyloxy)-N-{ [cis-4-({ [tert- butyl (dimethyl) silyl] oxy} methyl) cyclohexyl] methyl} benzamide. A mixture of 4- (benzyloxy)-N-{ [cis-4-({ [tert- butyl (dimethyl) silyl] oxy} methyl) cyclohexyl] methyl} benzamide and TBAF (1.0 M in THF, 30 mL) was stirred at room temperature for 4 hours. The mixture was diluted with AcOEt and was washed with 2 N aq. HCl and water, dried over MgSO4 and evaporated. The residue was purified by silica gel colomn chromatography (hexane-AcOEt 1: 3) to give the titled compound (1.9 g).

IH NMR (CDC13) 8 : 7.72 (d, J = 8.9 Hz, 2H), 7.46-7. 30 (m, 5 H), 7.00 (d, J = 8.9 Hz, 2H), 6.05-5. 95 (m, 1H), 5.11 (s, 2H), 3.56 (dd, J = 5.6, 6.8 Hz, 2H), 3.40 (dd, J = 5.9, 7.4 Hz, 2 H), 1.90-1. 40 (m, 10H) ppm. (OH was not observed.) Preparation 5 4- (Benzyloxy)-N-(( cis-4-f (4-methoxyphenoxy) methyllcyclohexylmethyl) benzamide Cyanomethylenetributylphosphorane (80 mg, 0.30 mmol) was added to a mixture of 4- (benzyloxy)-N-{[cis-4-(hydroxymethyl) cyclohexyl] methyl} benzamide (71 mg, 0.2 mmol), 4-methoxyphenol (37 mg, 0.30 mmol) in benzene (1.0 mL). The mixture was refluxed for 1 hour and purified by silica gel column chlomatography (hexane-AcOEt 4: 1) to give the titled compound (84 mg).

'H NMR (CDC13) 8 : 7.72 (d, J = 8.8 Hz, 2H), 7.46-7. 30 (m, 5H), 7.00 (d, J = 8.8 Hz, 2H), 6.83 (s, 4H), 6.10-6. 00 (m, 1H), 5.11 (s, 2H), 3.82 (d, J = 7.0 Hz, 2H), 3.77 (s, 3H), 3.41 (dd, J = 6.2, 7.1 Hz, 2H), 2.06-1. 40 (m, 10H) ppm.

Preparation 6 [cis-4- (Benzvloxv) cyclohexyllmethvl 4-methvlbenzenesulfonate Triflic acid (1.3 mL) was added to a mixture of (4-hydroxycyclohexyl) methyl 4- methylbenzenesulfonate (21 g, 75 mmol) (J. Org. Chem. 1970, 35, 2386-2390. ) and benzyl 2,2, 2-trichloroacetimidate (38 g, 0.15 mol) in CH2CI2at 0 °C and the mixture was stirred at room temperature for 16 hours. To the mixture, sat. aq. NaH3 was added and the mixture was extracted with CH2C12. The extract was dried over MgSO4 and evaporated. The residue was purified by silica gel column chlomatography (hexane-AcOEt 10: 1) to give the titled compound (13 g).

'H NMR (CDCI3) 8 : 7.78 (d, J = 8.4 Hz, 2H), 7.40-7. 10 (m, 7 H), 4.46 (s, 2H), 3.86 (d, J = 6.9 Hz, 2H), 3.65-3. 58 (m, 1H), 2.45 (s, 3H), 1.98-1. 24 (m, 9H) ppm.

Preparation 7 ( [cis-4- (Azidomethvl) cyclohexylloxv) methyl) benzene A mixture of [cis-4- (benzyloxy) cyclohexyl] methyl 4-methylbenzenesulfonate (14 g, 37 mmol) and sodium azide (12 g, 0.19 mol) in DMF (150 mL) was stirred at 85 °C for 3 hours.

The mixture was diluted with AcOEt and washed with water. The organic layer was dried over MgSO4 and evaporated. The residue was purified by silica gel column chlomatography (hexane: AcOEt = 20 : 1) to give the titled compound (6.9 g).

'H NMR (CDCI3) 8 : 7.36-7. 22 (m, 5H), 4.50 (s, 2 H), 3.68-3. 61 (m, 1H), 3.16 (d, J = 6.6 Hz, 2H), 2.04-1. 86 (m, 2H), 1.70-1. 36 (m, 7H) ppm.

Preparation 8 fcis-4- cyclohexyllmethylamine A solution of ({ [cis-4-(azidomethyl) cyclohexyl] oxy} methyl) benzene (6.9 g, 28 mmol) in THF (20 mL) was added dropwise to a suspension of LiAlH4 (1.6g, 42 mmol) in THF (140 mL) at 0 °C and the mixture was stirred at room temperature for 1 hour. The mixture was quenched with Na2SO4-10H2O (excess) and the white suspension was filtered. The filtrate was evaporated to give the titled compound (5.7 g).

'H NMR (CDCI3) 6 : 7.40-7. 22 (m, 5H), 4.50 (s, 2 H), 3.66-3. 60 (m, 1H), 2.58 (d, J= 5.7 Hz, 2H), 2.00-1. 30 (m, 9H) ppm. (NH2 was not observed.) Preparation 9 N- {[cis-4-(Benzyloxy)cyclohexyl]methyl}-4-(methoxymethoxy) benzamide A mixture of 4- (methoxymethoxy) benzoic acid (2.6 g, 14 mmol), [cis-4- (benzyloxy) cyclohexyl] methylamine (3.0 g, 14 mmol), EDCI (3.2 g, 17 mmol) and HOBt-H2O (0.43 g, 2.8 mmol) in DMF (70 mL) was stirred at room temperature for 16 hours. The mixture was diluted with AcOEt and was washed with sat. aq. NaHC03 and water, dried over MgS04 and evaporated. The residue was crystallized from CH2C12- diisopropylether to give the titled compound (4.2 g) as a white solid.

'H NMR (CDC13) 8 : 7.72 (d, J = 8.8 Hz, 2H), 7.38-7. 23 (m, 5H), 7.06 (d, J = 8. 8 Hz, 2H), 6.18-6. 08 (m, 1H), 5.21 (s, 2H), 4.50 (s, 2H), 3. 68-3. 62 (m, 1H), 3.48 (s, 3H), 3.34 (t, J = 6.4 Hz, 2H), 2.04-1. 90 (m, 2H), 1.75-1. 40 (m, 7H) ppm.

Preparation 10 N(cis-4-Hydroxycyclohexyl) methyll-4- (methoxvmethoxy benzamide A mixture of N-{[cis-s (benzyloxy) cyclohexyllmethyl}-4-(methoxymethoxy) benzamide (4.0 g, 10 mmol) and 20% Pd (OH) 2-C (0.50 g) in EtOH (200 mL) was hydrogenated under hydrogene atomsphere at 4 atm at room temperature for 8 h. The mixture was filtered by celite and evaporated. The titled compound (2.9 g) was afforded by crystallization from CH2C12-diisopropylether as a white solid.

'H NMR (CDCI3) 8 : 7.73 (d, J = 8.2 Hz, 2H), 7.06 (d, J = 9.0 Hz, 2H), 6. 20-6. 10 (m, 1H), 5.22 (s, 2H), 4.05-3. 98 (m, 1H), 3. 48 (s, 3H), 3.35 (t, J = 6.6 Hz, 2H), 1.84-1. 36 (m, 9H) ppm. (OH was not observed.) Preparation 11 trans-4- (if4- (Methoxymethoxv) benzoyll aminomethyl ? cyclohexyl benzoate A mixture of N-[(cis-4-hydroxycyclohexyl)methyl]-4-(methoxymethoxy)benzam ide (0.58 g, 2.0 mmol), benzoic acid (0.37 g, 3.0 mmol) and cyanomethylenetributylphosphorane (0.80 g, 3.0 mmol) in benzene (10 mL) was refluxed for 4 hours. The residue was purified by silica gel column chlomatography (hexane: AcOEt = 3: 1) to give the titled compound (0.28 g).

'H NMR (CDC13) 6 : 8. 06-8.00 (m, 2H), 7.75 (d, J = 8.8 Hz, 2H), 7.50-7. 40 (m, 3H), 7.07 (d, J = 8.8 Hz, 2H), 6.32-6. 20 (m, 1H), 5.22 (s, 2H), 5.00-4. 87 (m, 1H), 3.48 (s, 3H), 3.34 (t, J = 6.4 Hz, 2H), 2.20-2. 10 (m, 2H), 1.98-1. 88 (m, 2H), 1.78-1. 42 (m, 3H), 1.30-1. 12 (m, 2H) ppm.

Preparation 12 N-[(trans-4-Hydroxycyclohexyl)methyl]-4-(methoxymethoxy) benzamide A mixture of trans-4- ( { [4- (methoxymethoxy) benzoyl] amino} methyl) cyclohexyl benzoate (0.24 g, 0.61 mmol), 2N aq. NaOH (3 mL), MeOH (3 mL) and THF (3 mL) was stirred at room temperature for 1 hour. The mixture was diluted with water and was extracted with CH2C12. The extract was dried over MgS04 and evaporated to give the titled compound (0.17 g).

'H NMR (CDCI3) 8 : 7.72 (d, J = 8.8 Hz, 2H), 7.07 (d, J = 8.6 Hz, 2H), 6.15-6. 00 (br, 1H), 5.22 (s, 2H), 3.65-3. 50 (m, 1H), 3. 48 (s, 3H), 3.31 (t, J = 6.6 Hz, 2H), 2.13-1. 95 (m, 2H), 1.91-1. 80 (m, 2H), 1.65-1. 00 (m, 5H) ppm. (OH was not observed.) Preparation 13 4- (Methoxvmethoxy)-N-f fcis-4- (4-methoxyphenoxv) cyclohexyllmethvl benzamide A mixture of N-[(trans-4-hydroxycyclohexyl)methyl]-4-(methoxymethoxy) benzamide (30 mg, 0.10 mmol), 4-methoxyphenol (19 mg, 0.15 mmol) and cyanomethylenetributylphosphorane (40 mg, 0.15 mmol) in benzene (0.5 mL) was refluxed for 4 hours. The mixture was purified by silica gel column chlomatography (hexane: AcOEt = 3: 1) to give the titled compound (11 mg).

'H NMR (CDCI3) 6 : 7.72 (d, J = 8. 9 Hz, 2H), 7.07 (d, J = 8. 7 Hz, 2H), 6.90-6. 80 (m, 4H), 6.20-6. 05 (m, 1H), 5.22 (s, 2H), 4.46-4. 38 (m, 1H), 3.77 (s, 3H), 3.48 (s, 3H), 3.36 (t, J = 6.4 Hz, 2H), 2.15-1. 40 (m, 9H) ppm.

Preparation 14 N [cis-4- (4-Chlorophenoxy) cvclohexvllmethyl -4- (methoxymethoxv) benzamide This compound was prepared with 4-chlorophenol by a procedure similar to that in Preparation 13.

IH NMR (CDCI3) 6 : 7.72 (d, J = 8.8 Hz, 2H), 7.21 (d, J = 9.2 Hz, 2H), 7.07 (d, J = 8.8 Hz, 2H), 6.83 (d, J = 9.0 Hz, 2H), 6.20-6. 10 (m, 1 H), 5.22 (s, 2H), 4.53-4. 46 (m, 1H), 3.48 (s, 3H), 3.36 (t, J = 6.4 Hz, 2H), 2.10-2. 00 (m, 2H) 1.80-1. 40 (m, 7H) ppm.

Preparation 15 1- (Aminomethvl)-4- (phenoxymethyl) cvclohexanol hydrochloride 4- (Phenoxymethyl) cyclohexanone (5.0 g, 24 mmol) (Tetrahedron 1969, 25, 2159-2192. ) was added to a mixture of trimethylsilylcyanide (3.5 mL, 26 mmol) and zinc iodide (0.38 g, 1.2 mmol) in toluene (48 mL) at-78 °C. The mixture was stirred at 0 °C for 4 hours. The mixture was added dropwise to a suspension of LiAIH4 (1.8 g) in THF (100 mL) at 0 °C and the mixture was stirred at room temperature for 2 hours. The mixture was quenched with excess of Na2SO4#10H2O and stirred for 4 hours. After filtration, the filtrate was concentrated to give 1- (aminomethyl)-4- (phenoxymethyl) cyclohexanol. 4N HCl in AcOEt (7 mL) was added to a solution of 1- (aminomethyl)-4- (phenoxymethyl) cyclohexanol in EtOH (30 mL) and the mixture was concentrated. The residue was crystallized from MeOH (15 mL) to give 1- (aminomethyl)-4- (phenoxymethyl) cyclohexanol hydrochloride (4.9 g).

'H NMR (DMSO-d6) 6 : 7.93 (br, 3H), 7.32-7. 24 (m, 2 H), 6.96-6. 88 (m, 3H), 5.08 (br, IH), 3.83 (d, J = 6. 1 Hz, 2H), 2.83 (s, 2H), 1.85-1. 70 (m, 5H), 1.50-1. 12 (m, 4H) ppm.

Preparation 16 trans-1-(Aminomethyl)-4-f (benzeloxy) methyllcyclohexanol hedrochloride 4-[(Benzyloxy) methyl] cyclohexanone (7.5 g, 34 mmol) (J. Med. Chef. 1993, 36, 654- 670) was added to a mixture of Znk (0.54 g, 1.7 mmol) and TMSCN (4.8 mL, 36 mmol) in toluene (34 mL) at-78 °C and the mixture was stirred at-78 °C for 3 hours. The mixture was dropwised to a suspension of LiAIH4 (2.6 g, 68 mmol) in THF (136 mL) at 0 °C and the mixture was stirred at room temperature for 2 hours. The mixture was quenched with Na2SO4 10H2O (excess) and stirred for 4 hours. After filtration, the filtrate was evaporated.

The residue was dissolved with ethanol and 4N HCl in AcOEt (10 mL) was added at 0 °C. The sovent was removed in vacuum. The residue was crystallized from ethanol to afford the titled compound (6.1 g) as a white solid.

IH NMR (DMSO-d6) 8 : 7.93 (br, 3H), 7. 38-7. 24 (m, 5H), 5.07 (br, 1H), 4.45 (s, 2H), 3.28 (d, J= 6.0 Hz, 2H), 2.79 (s, 2H), 1. 75-1. 00 (m, 9H) ppm.

Preparation 17 N-({trans-4-[(Benzyloxy)methyl]-1-hydroxycyclohexyl}methyl)- 4- (methoxymethoxy) benzamide A mixture of 4- (methoxymethoxy) benzoic acid (4.0 g, 22 mmol), trans-1- (aminomethyl)-4-[(benzyloxy) methyl] cyclohexanol hydrochloride (6.1 g, 21 mmol), Et3N (5.9 mL, 42 mmol), EDCI (4. 8 g, 25 mmol) and HOBt-H2O (0.64 g, 4.2 mmol) in DMF (60 mL) was stirred at room temperature for 16 hours. The mixture was diluted with AcOEt and washed with sat. aq. NaHC03 and water, dried over MgSO4, and evaporated. The residue was crystallized from CH2C12-hexane to afford the titled compound (7.2 g) as a white solid.

IH NMR (CDC13) 8 : 7.75 (d, J = 8. 9 Hz, 2H), 7.96-7. 26 (m, SH), 7.07 (d, J = 8. 9 Hz, 2H), 6.56-6. 46 (m, 1H), 5.22 (s, 2H), 4.49 (s, 2H), 3.57 (d, J = 5.9 Hz, 2H), 3.48 (s, 3H), 3.33 (d, J= 6.4 Hz, 2H), 2.41 (s, 1H), 1. 90-1. 10 (m, 9H) ppm.

Preparation 18 N-{[trans-1-Hydroxy-4-(hydroxymethyl)cyclohexyl]methyl}-4- (methoxYmethoxy) benzamide A mixture of N-({trans-4-[(benzyloxy) methyl]-1-hydroxycyclohexyl} methyl)-4- (methoxymethoxy) benzamide (6.5 g, 16 mmol) and 20% Pd (OH) 2-C (0.5 g) in EtOH (160 mL) was hydrogenated under 4 atm at room temperature for 4 hours and at 60 °C for 4 hours. The mixture was filtered through a pad of celite and the filtrate was evaporated. The residue was purified by silica gel column chlomatography (CH2C12 : MeOH = 12: 1) to give the titled compound (4.5 g) as a white solid.

'H NMR (DMSO-d6) 8 : 8.02 (t, J = 5.9 Hz, 1H), 7.84 (d, J = 8.6 Hz, 2H), 7.07 (d, J = 8.8 Hz, 2H), 5.25 (s, 2H), 4.64 (s, 1H), 4.40 (t, J = 5.1 Hz, 1H), 3.39-3. 30 (m, 5H), 3.24 (d, J = 5.9 Hz, 2H), 1.68-1. 55 (m, 4H), 1.44-1. 02 (m, 5H) ppm.

Preparation 19 8-r2-(Benzyloxy) ethyll-1, 4-dioxaspiror4. 51decane To a solution of 2- (1, 4-dioxaspiro [4.5] dec-8-yl) ethanol (2.0 g, 11 mmol) (J. Am. Cizem.

Soc. 1991, 113, 8016-8024.) in DMF (20 mL), NaH (60%, 0.48 g, 12 mmol) was added at 0 °C and the mixture was stirred at room temperature for 3 hours. The mixture was quenched with water and the whole was extracted with AcOEt. The organic layer was washed with water, dried over MgSO4, and evaporated. The residue was purified by silica gel column chlomatography (hexane: AcOEt = 10: 1) to give the titled compound (2.2 g).

'H NMR (CDCl3) 8 : 7.40-7. 22 (m, 5H), 4.50 (s, 2H), 3.93 (s, 4H), 3.50 (t, J = 6.4 Hz, 2H), 1.80-1. 16 (m, HH) ppm.

Preparation 20 4-f2- (Benzyloxy) ethvllcvclohexanone A mixture of 8-[2-(benzyloxy) ethyl]-1, 4-dioxaspiro [4.5] decane (2.2 g, 8.0 mmol) and 2N aq. HCl (40 mL) in THF was stirred at 50 °C for 3 hours. The mixture was extracted with AcOEt and the extract was washed with sat. aq. NaHC03 and water, dried over MgS04 and evaporated to give 4- [2- (benzyloxy) ethyl] cyclohexanone (1.9 g).

1H NMR (CDC13) 8 : 7.40-7. 25 (m, 5H), 4.52 (s, 2H), 3.54 (t, J= 6.3 Hz, 2H), 2.45-1. 30 (m, 11H) ppm.

Preparation 21 trans-1- (Aminomethyl)-4-f2- (benzvloxv) ethyllcyclohexanol hvdrochloride A solution of 4- [2- (benzyloxy) ethylleyelohexanone (1.9 g) in toluene (16 mL) was added to a mixture ofZnl2 (0.13 g, 0.40 mmol) and TMSCN (1.2 mL, 8. 8 mmol) in toluene (10 mL), at-78 °C and the mixture was stirred at-78 °C for 2 hours. The mixture was dropwised to a suspension of LiAlH4 (0.61 g, 16 mmol) in THF (40 mL) at 0 °C and stirred at room temperature for 1 hour. The mixture was quenched with Na2SO4-1OH20 (excess) and KF (excess). After filtration, the filtrate was evaporated. The residue was dissolved with ethanol and 4N HCI in AcOEt (3 mL) was added at 0 °C. The sovent was removed in vacuum. The residue was crystallized from ethanol to afford the titled compound (1.5 g) as a white solid.

'H NMR (DMSO-d6) 8 : 7.89 (br, 3H), 7.40-7. 24 (m, 5H), 5.00 (br, 1H), 4.44 (s, 2H), 3.44 (t, J= 6.3 Hz, 2H), 2.79 (s, 2H), 1. 75-1. 00 (m, 11H) ppm.

Preparation 22 N-( (trans-4-r2- (Benzyloxy) ethyll-1-hydroxycyclohexyl} methyl)-4- (methoxymethoxy) benzamide This compound was prepared with trans-1- (aminomethyl)-4- [2- (benzyloxy) ethyl] cyclohexanol hydrochloride by a procedure similar to that in Preparation 17.

IH NMR (CDCl3) 8 : 7.75 (d, J = 8. 8 Hz, 2H), 7.38-7. 24 (m, 5H), 7.06 (d, J = 8. 8 Hz, 2H), 6.55 (t, J = 5.5 Hz, 1H), 5.20 (s, 2H), 4.49 (s, 2H), 3.58-3. 45 (m, 7H), 2.42 (br, 1H), 1. 86- 1.05 (m, I IH) ppm.

Preparation 23 N-f [trans-1-Hvdroxv-4 (2-hydroxvethyl) cyclohexyllmethyl}-4- (methoxymethoxy) benzamide A mixture of N-({trans-4-[2-(benzyloxy) ethyl]-1-hydroxycyclohexyl} methyl)-4- (methoxymethoxy) benzamide (1.4 g, 3.2 mmol) and 20% Pd (OH) 2-C (0.50 g) in EtOH (60 mL) was hydrogenated under 4 atm at 8 hours. The mixture was filtered through a pad of celite and the filtrate was evaporated. The residue was purified by silica gel column chlomatography (hexane-AcOEt 1: 2 to AcOEt only) to give the titled compound (1.0 g). lH NMR (CDC13) 8 : 7.76 (d, J = 8.9 Hz, 2H), 7.06 (d, J = 9.0 Hz, 2H), 6.65-6. 53 (m, 1H), 5.21 (s, 2H), 3.73-3. 64 (m, 2H), 3.58 (d, J = 5.9 Hz, 2H), 3.48 (s, 3H), 2.43 (br, 1H), 1.88- 1. 10 (m, HH) ppm.

Preparation 24 1-(Aminomethyl)-4-(benzyloxy)cyclohexanol hydrochloride 4- (Benzyloxy) cyclohexanone (19 g, 94 mmol) (J. Org. Chemin. 1982, 47, 3881-3886.) was added dropwise to a mixture of Znk (1.5 g, 4.7 mmol) and TMSCN (13 mL, 98 mmol) in toluene (100 mL) at 0 °C and the mixture was stirred at room temperature for 2 hours. The mixture was dropwised to a suspension of LiAIH4 (8.5 g, 98 mmol) in THF (400 mL) at 0 °C and the mixture was stirred at room temperature for 2 h. The mixture was quenched with Na2SO4-10H2O (excess) and stirred for 4 hours. After filtration, the filtrate was evaporated. The residue was dissolved with ethanol and 4N HCI in AcOEt (25 mL) was added at 0 °C. The sovent was removed in vacuum. The residue was crystallized from ethanol to afford the titled compound (6.1 g) as a white solid.

1H NMR (DMSO-d6) 6 : 8.00 (br, 3H), 7.40-7. 20 (m, 5H), 4.91 (br, 1H), 4. 82-4. 44 (m, 2H), 3.60-3. 24 (m, 1H), 2.80-2. 65 (m, 2H), 1. 85-1. 20 (m, 8H) ppm.

Preparation 25 Ethyl 4-f (2- (trimethvlsilyl) ethoxylmethoxy} benzoate To a mixture of ethyl 4-hydroxybenzoate (4.3 g, 26 mmol) and i-Pr2NEt (5.4 mL, 31 mmol) in CH2Cl2 (52 mL), SEMCI (5.0 mL, 28 mmol) was added at 0 °C and the mixture was stirred at room temperature for 72 hours. The mixture was diluted with CH2CI2. The whole was washed with sat. aq. NH4CI, dried over MgS04, and evaporated to give ethyl 4- { [2- (trimethylsilyl) ethoxy] methoxy} benzoate (9.0 g).

'H NMR (CDC13) 8 : 7.99 (d, J = 9.1 Hz, 2H), 7.05 (d, J = 8.9 Hz, 2H), 5.27 (s, 2H), 4.35 (q, J = 7.3 Hz, 2H), 3.79-3. 71 (m, 2H), 1.38 (t, J = 7.1 Hz, 3H), 0.99-0. 89 (m, 2H), -0. 01 (s, 9H) ppm.

Preparation 26 4-{[2-(Trimethylsilyl)ethoxy]methoxy}benzoic acid A mixture of ethyl 4-{[2-(trimethylsilyl) ethoxy] methoxy} benzoate (9.0 g) and 8N aq.

KOH (20 mL) in EtOH (50 mL) was stirred at room temperature for 6 hours. The mixture was acidified with c. HCI at 0 °C. The precipitate was filtered and washed with water to give the titled compound (6.7g) as a white crystal.

1H NMR (CDC13) 8 : 8.07 (d, J = 8.9 Hz, 2H), 7.09 (d, J = 9.0 Hz, 2H), 5.29 (s, 2H), 3. 81- 3.72 (m, 2H), 1.00-0. 92 (m, 2H), 0.00 (s, 9H) ppm.

Preparation 27 N-{[4-(Benzyloxy)-1-hydroxycyclohexyl]methyl}-4-{[2- ftrimethylsilyl) ethoxy]methoxy}benzamide A mixture of 4- { [2- (trimethylsilyl) ethoxy] methoxy} benzoic acid (4.0 g, 15 mmol), 1- (aminomethyl)-4- (benzyloxy) cyclohexanol hydrochloride (4.1 g, 15 mmol), Et3N (4.2 mL, 30 mmol), EDCI (3.5 g, 18 mmol) and HOBt-H2O (0.46 g, 3.0 mmol) in DMF (45 mL) was stirred at room temperature for 16 hours. The mixture was diluted with AcOEt. The whole was washed with sat. aq. NaHC03 and water, dried over MgSO4 and evaporated to give the titled compound (7.8 g) as a white solid.

IH NMR (CDCl3) 6 : 7.75 (d, J = 8. 6 Hz, 2H), 7.38-7. 22 (m, 5H), 7.07 (d, J = 8.6 Hz, 2H), 6.57-6. 45 (m, 1H), 5.26 (s, 2H), 4.58-4. 50 (m, 2H), 3.82-3. 34 (m, 5H), 2.00-1. 30 (m, 8H), 1.00-0. 91 (m, 2H), 0.00 (s, 9H) ppm.

Preparation 28 8-(4-Chlorophenoxy)-1. 4-dioxaspiroF4. 5ldecane DIAD (12 mL, 60 mmol) was added dropwise to a mixture of 1, 4-dioxaspiro [4.5] decan- 8-ol (6.3 g, 40 mmol) (J. Chem. Soc., Perkin Trans. 1, 2002,2251-2255.), 4-chlorophenol (7.7 g, 60 mmol) and triphenylphosphine (16 g, 60 mmol) in THF (200 mL) at 0 °C and the mixture was stirred at room temperature for 16 hours. After evaporation, the residue was treated with 2N aq. NaOH and the whole was extracted with CH2C12. The extract was dried over MgS04 and evaporated. The residue was purified by silica gel column chlomatography (hexane: AcOEt = 20: 1 to 5 : 1) the titled compound (6.8 g).

1H NMR (CDC13) 6 : 7.22 (d, J = 9.2 Hz, 2H), 6.84 (d, J = 9.0 Hz, 2H), 4.40-4. 32 (m, 1H), 3.98-3. 94 (m, 4H), 1.96-1. 84 (m, 6H), 1.68-1. 55 (m, 2H) ppm.

Preparation 29 4- (4-Chlorophenoxv) cvclohexanone A mixture of 8- (4-chlorophenoxy)-1, 4-dioxaspiro [4.5] decane (6. 8 g, 25 mmol) and 2N aq. HC1 (50 mL) in acetone (80 mL) was refluxed for 3 hours. The mixture was diluted with AcOEt. The whole was washed with sat. aq. NaCl and sat. aq. NaHCO3, dried over MgS04 and evaporated to give 4- (4-chlorophenoxy) cyclohexanone (5.6 g).

'H NMR (CDC13) 6 : 7.26 (d, J = 9.0 Hz, 2H), 6.90 (d, J = 9.0 Hz, 2H), 4.70-4. 62 (m, 1H), 2.74-2. 60 (m, 2H), 2.39-2. 00 (m, 6H) ppm.

Preparation 30 1- (Aminomethyl)-4- (4-chlorophenoxy) cyclohexanol 4- (4-Chlorophenoxy) cyclohexanone (5.6 g) was added to a mixture of ZnI2 (80 mg, 0.25 mmol) and TMSCN (2.8 g, 28 mmol) in benzene (10 mL) at 0 °C and the mixture was stirred at room temperature for 30 min. The mixture was added dropwise to a suspension of LiAlH4 (2.3 g, 60 mmol) in ether (80 mL) at 0 °C and the mixture was stirred at room temperature for 2 hours. The mixture was quenched with Na2SO4#10H2O (excess) and the white suspension was filtered. After the filtrate was evapotated to give the titled compound (6. 8 g) as cis-trans mixture.

IH NMR (CDCI3) 8 : 7.26-7. 18 (m, 2H), 6.87-6. 80 (m, 2H), 4.55-4. 10 (m, 1H), 2.66-2. 62 (m, 2H), 2.16-1. 20 (m, 8H) ppm. (OH and NH2 were not observed) Preparation 31 2- (Iodomethvl) tetrahydro-2H-pyran-5-yllmethanol To a suspension of Tz (16 g, 63.5 mmol) and NaHC03 (5.3 g, 64 mmol) in ether (70 mL) and H2O (33 mL) was added a solution of 2-(hydroxymethyl)-5-hexene-1-ol (5.5 g, 42 mmol) in ether (40 mL) at 0 °C. The mixture was stirred at room temperture for 8 hours.

Then the reaction was quenched by addition of sat. aq. Na2S203 at 0 °C. The aqueous layer was extracted with ether (50 mL x 2) and the combined organic layers were washed with brine (50 mL), dried over Na2S04, filtered and concentrated in vacuo. The crude product was purified by silica gel column chromatography (hexane: AcOEt = 1.2 : 1) to give the titled compound (8.2 g, 75%) as yellow oil.

'H NMR (300 MHz, CDC13, cisltrans mixture) 6 : 4. 18-3. 15 (m, 7H), 1.92-1. 29 (m, SH) ppm. (OH was not observed.) 13C NMR (75 MHz, CDC13, trans-major isomer) 8 : 76.9, 71.2, 64.5, 38. 3, 31.0, 26.2, 9.5 ppm.

13C NMR (75 MHz, CDC13, cis-minor isomer) 8 : 76.8, 68.7, 62.9, 35.5, 27.5, 24.0, 10.0 ppm.

MS (ESI) : 257.0 (M+H) + Preparation 32 N-d f5- (Hvdroxymethyl) tetrahydro-2H-pyran-2-yllmethl -phthalimide To a solution of 2-[(iodomethyl) tetrahydro-5-2H-pyran-5-yl] methanol (1.8 g, 6.9 mmol) in DMF (45 mL) was added potassium phthalimide (1. 8 g, 9.7 mmol) at rt and the mixture was stirred at 90 °C. After 5 hours the mixture was cooled to rt and to this mixture was added H2O (50 mL). The whole was extracted with AcOEt (100 mL X 2). The organic layers were washed with H20 (50 mL X 2), brine (50 mL), dried over Na2S04, filtered and concentrated in vacuo. The crude product was purified by silica gel column chromatography (hexane: AcOEt = 1: 1.2) to give the titled compound (1.3 g, 68%) as a white solid "C NMR (75 MHz, CDCl3) 6 : 168.3 (major/minor), 133.9 (major or minor), 133.8 (major or minor), 131.9 (major/minor), 123.2 (major/minor), 74.9 (major), 74.6 (minor), 70.9 (major), 67.9 (minor), 64.5 (major), 62.5 (minor), 42.5 (major), 42.1 (minor), 38. 3 (major), 35.7 (minor), 28.8 (major), 26.0 (major), 25.2 (minor), 23.6 (minor) ppm.

MS (ESI) : 276.1 (M+H) + Preparation 33 N-f [5-(Phenoxemethylitetrahydro-2H-pyran-2-yllmethyl}-phthalimi de To a mixture of N-{[5-(hydroxymethyl)tetrahydro-2H-pyran2-yl]methyl}-phthali de (1.2 g, 4.2 mmol), phenol (0.47 g, 5.0 mmol) and PPh3 in THF (20 mL) was added DEAD (40 % in toluene, 2.7 g, 6.3 mmol) at 0 °C and the mixture was stirred at room temperture for 15 hours. Then the reaction mixture was quenched by addition of H20 (50 mL) and diluted with AcOEt (50 mL). The aqueous layer was extracted with AcOEt (50 mL) and the combined organic layers were washed with brine (50 mL), dried over Na2S04, filtered and concentrated in vacuo. The crude product was purified by silica gel column chromatography (hexane: AcOEt = 8: 1-4 : 1) to give the titled compound (0.67 g, 45%).

'H NMR (300 MHz, CDCI3, cisltrans mixture) 6 : 7.88-7. 70 (m, 4H), 7.31-7. 23 (m, 2H), 6.96-6. 82 (m, 3H), 4.16-3. 57 (m, 6H), 3.22-3. 15 (m, 1H), 2.15-1. 73 (m, 2H), 1.52-1. 26 (m, 3H) ppm.

Preparation 34 f [5- (Phenoxymethyl) tetrahvdro-2H-pyran-2-yllmethyl) aniin To a suspension of N-{ [5-(phemoxymethyl) tetrahydro-2H-pyran-2-yl] methyl}- phthalimide (0.67 g, 1.90 mmol) in EtOH (10 mL) was added hydrazine hydrate (0.14 g, 2.9 mmol) and the mixture was refluxed for 3 hours. After evaporation 10% aq. NaOH (50 mL) was added and the mixture was stirred for 30 min. Then the aqueous layer was extracted with CHC13 (30 mLX3). The combined organic layers were dried over Na2S04, filtered and concentrated in vacuo to give the titled compound (0.44 g, crude).

'H NMR (300 MHz, CDCl3, cis/trans mixture) 8 : 7.31-7. 24 (m, 2H), 6.96-6. 86 (m, 3H), 4.23-3. 99 (m, 2H), 3.84-3. 63 (m, 2H), 3. 31-3. 21 (m, 1H), 2.74-2. 64 (m, 2H), 2.17-1. 21 (m, 5H) ppm. (NHz was not observed.) MS (ESI): 222.1 (M+H) + Preparation 35 4-(Methoxymethoxy)-N-{[5-phenoxymethyl]tetrahydro-2H-pyran-2 -yl}methyl}benzamide This compound was prepared with { [5- (phenoxymethyl) tetrahydro-2H-pyran-2- yl] methyl} amine by a procedure as a white solid similar to that in Preparation 9.

IH NMR (300 MHz, CDC13, cisltrans mixture) 8 : 7.77-7. 72 (m, 2H), 7.31-7. 25 (m, 2H), 7.08-6. 86 (m, 5H), 5.22 (s, 2H), 4.22-3. 98 (m, 3H), 3.85-3. 63 (m, 3H), 3. 48 (s, 3H), 3.43- 3.18 (m, 2H), 2.17-1. 36 (m, 5H) ppm.

MS (ESI) : 386.17 (M+H) + Preparation 36 N f5- (Benzyloxymethyl) tetrahydro-2H-pyran-2-yllmethyl}-phthalimide To a solution of N-{[5-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl}-phthal imide (1.3 g, 4.7 mmol) in CH2C12 (20 mL) were added Ag2O (2.2 g, 9.4 mmol) and BnBr (0.84 mL, 7.1 mmol) at rt. After 50 hours, the mixture was filtered through a pad of celite and the filtrate was concentrated in vacuo. The crude product was purified by silica gel column chromatography (hexane-AcOEt 5: 1) to give the titled compound (1.6 g, 92%) as a white solid.

3C NMR (75 MHz, DMSO) 8 : 168.4 (major), 168.3 (minor), 138.4 (minor), 138.3 (major), 133. 8 (major/minor). 132.0 (major/minor), 128.3 (major/minor), 127.5 (minor), 127.4 (major), 127.3 (major/minor), 123.2 (major/minor), 74.9 (major), 74.5 (minor), 73.1 (minor), 72.8 (major), 71.9 (major), 71.2 (major), 70.2 (minor), 68.3 (minor), 42.6 (major), 42. 1 (minor), 36.0 (major), 33.9 (minor), 28.9 (major), 26.4 (major), 25.2 (minor), 23.7 (minor) ppm.

Preparation 37 f 5- (Benzvloxymethyl) tetrahvdro-2H-pyran-2-yllmethylamine This compound was prepared with N-{[5-(benzyloxymethyl)tetrahydro-2H-pyran-2- yl] methyl}-phthalimide by a procedure similar to that in Preparation 34.

'H NMR (300 MHz, CDC13, cis/trans mixture) 6 : 7.37-7. 28 (m, 5H), 4.60-4. 43 (m, 2H), 4.14-3. 97 (m, 1H), 3.74-3. 50 (m, 1H), 3.33-3. 14 (m, 3H), 2.73-2. 66 (m, 2H), 1.99-1. 17 (m, SH) ppm. (NH2 was not observed.) MS (ESI) : 236.1 (M+H) +.

Preparation 38 4-(Benzyloxymethoxy)-N-{[5-phenoxymethyl[]tetrahydro-2H-pyra n-2-yl}methyl}benzamide This compound was prepared with { [5-(benzyloxymethyl) tetrahydro-2H-pyran-2- yl] methyl} amine by a procedure similar to that in Preparation 9.

'H NMR (300 MHz, CDC13, cis/trans mixture) 8 : 7.76-7. 71 (m, 2H), 7. 28-7. 37 (m, SH), 7.04-7. 09 (m, 2H), 4.60-4. 43 (m, 2H), 3.83-3. 75 (m, 1H), 3. 48 (s, 3H), 3. 31-3. 14 (m, 3H), 1.97-1. 31 (m, 5H) ppm.

MS (ESI) : 400.2 (M+H) +.

Preparation 39 2-(Benzyloxymethyl)-hex-5-en-1-ol To a solution of 2- (hydroxymethyl)-hex-5-enl-ol in CH2C12 were added BnBr (3.8 mL, 44 mmol) and Ag2O (10 g, 44 mmol) at rt for 10 hours. Then the mixture was filtered through a pad of celite and the filtrate was ceoncentrated in vacuo. The crude product was purified by silica gel column chromatography (hexane-AcOEt 7: 1) to give the titled compound (5.0 g, 78%) as colorless oil.

1H NMR (300 MHz, CDCl3) 8 : 7. 38-8. 29 (m, 5H), 5.86-5. 71 (m, 1H), 5.04-4. 94-7.24 (m, 2H), 4.55 (d, J = 12.2 Hz, 1H), 4.49 (d, J = 12.2 Hz, 1H), 3.45-3. 77 (m, 4H), 2. 12-2. 07 (m, 2H), 1. 93-1.85 (m, 1H), 1.50-1. 32 (m, 2H) ppm.

Preparation 40 2-Benzyloxvmethyl-4-oxiran-2-ylbutan-1-ol To a solution of 2-(benzyloxymethyl)-hex-5-en-1-ol in CH2Cl2 were added NaHC03 and meta-chlor-prbenzoic acid (mCPBA) at 0 °C. After 7 hours, the reaction was quenched by addition of sat. a. NaHC03 (50 mL). The aqueous layer was extracted with CH2C12 and the combined organic layers were washed with brine (50 mL), dried over MgSO4, filtered and concentrated in vacuo. The crude product was purified by silica gel column chromatography (hexane-AcOEt 3: 1-1 : 1) to give the titled compound (2.6 g, 60%) as pale yellow oil H NMR (300 MHz, CDCI3) 5 : 7. 28-7. 38 (m, 5H), 4.54 (d, J = 12.0 Hz, 1H), 4.50 (d, J = 12.0 Hz, 1H), 3.76-3. 46 (m, 4 H), 2.92-2. 87 (m, 1H), 2.75 (dt, J= 0.7, 4.9 Hz, 1H), 2.47 (ddd, J= 0.9, 2.7, 4.9 Hz, 1H), 1.70-1. 94 (m, 1H), 1.69-1. 35 (m, 4H) ppm.

MS (ESI) : 237.1 (M+H) +.

Preparation 41 f 5-f (BenzYloxy) methylltetrahydro-2H-pyran-2-yl Wmethanol To a solution of 2-benzyloxymethyl-4-oxiran-2-ylbutan-1-ol in CH2Cl2 was added BF3#OEt2 at -78 °C and the mixture was warmed to 0 °C. After 4 hours, the reacton was quenched by addition of H20 (50 mL). The aqueous layer was extracted with CH2CI2 (50 mL) and the combined organic layers were washed with brinie (50 mL), dried over MgS04, filtered and concentrated in vacuo. The crude product was purified by silica gel column chromatography (hexane-AcOEt 3: 1) to give the titled product (1.5 g including unknown impurity) 1H NMR (300 MHz, CDCl3, cisltrans mixture) b : 7.37-7. 09 (m, 5H), 4.60-4. 42 (m, 4 H), 3.89-4. 16 (m, 2H), 3.70-3. 17 (m, 5H), 2.00-1. 18 (m, 5H) ppm. (OH was not observed.) Preparation 42 5-f (Benzyloxv) methvll-2- (phenoxymethvl) tetrahydro-2H-pyran To a mixture of {5-[(benzyloxy)methyl]tetrahydro-2H-pyran-2-yl}methanol (1.5 g, 6.2 mmol), phenol (0.7 g, 7.4 mmol) and PPh3 (2.0 g, 7.4 mmol) in THF (25 mL) was added DEAD (diethylazodicarboxylate) (40% in toluene, 4.0 g) at 0 °C and the mixture was stirred at rt for 14 hours. Then the reaction was quenched by addition of H2O (50 mL) and extracted with AcOEt (50 mL x 2). The combined organic layers were washed with brine (50 mL), dried over MgSO4, filtered and concentrated in vacuo. The crude product was purified by silica gel column chromatography (hexane: AcOEt = 12: 1) to give the titled product (0.30 g, 15%) 'H NMR (300 MHz, CDC13, cisltrans mixture) 8 : 7.37-7. 24 (m, 7H), 6.96-6. 90 (m, 3H), 4.63-4. 42 (m, 2H), 4.20-3. 54 (m, 5H), 3.35-3. 22 (m, 2H), 2.02-1. 72 (m, 3H), 1.55-1. 23 (m, 2H) ppm.

MS (ESI) : 313.2 (M+H) +.

Preparation 43 [6-(Phenoxymethyl)tetrahydro-2H-pyran-3-yl]methanol To a mixture of 5-L (benzyloxy) methyll-2-(phenoxymethyl) tetrahydro-2H-pyran (0.3 g, 0.95 mmol) and Pd (OH) 2/C (20 wt. % Pd on carbon, 0.15 g) in THF (5 mL) was added 10-20% HCl-MeOH (0.5 mL). The mixture was stirred under H2 atmosphere (4 atm) at rt for 5 hours. Then the mixture was filtered through a pad of celite and the filtrate was concentrated in vacuo. The crude product was purified by silica gel column chromatography (hexane: AcOEt = 2: 1) to give the titled compound (0.16 g, 77%) 'H NMR (300 MHz, CDCl3, cis-trans mixture) 8 : 7.31-7. 25 (m, 2H), 6.97-6. 90 (m, 3H), 4.21-3. 23 (m, 7H), 1.98-1. 27 (m, 5H) ppm. (OH was not observed) MS (ESI): 223.0 (M+H) +.

Preparation 44 f6- (Phenoxvmethvl) tetrahydro-2H-pyran-3-yllmethyl methanesulfonate Methanesulfonyl chloride (68 L, 0. 88 mmol) was added to a mixture of [6- (phenoxymethyl) tetrahydro-2H-pyran-3-yl] methanol (0.16 g, 0.73 mmol) and triethylamine (0.20 mL, 1.5 mmol) in CH2CI2 at 0 °C and the mixture was stirred at 0 °C for 2 hours. The mixture was treated with sat. aq. NaHC03 and was extracted with CH2CI2. The extract was dried over MgSO4 and evaporated to give [6-(phenoxymethyl) tetrahydro-2H-pyran-3- yl] methyl methanesulfonate (0.20 g).

'H NMR (CD3) 5 : 7.32-7. 24 (m, 2H), 7.00-6. 88 (m, 3H), 4.50-3. 24 (m, 7H), 3.04-3. 01 (m, 3H), 2.20-1. 30 (m, 5H) ppm.

Preparation 45 5-(Azidomethyl)-2-(phenoxymethyl)tetrahydro-2H-pyran [6- (Phenoxymethyl) tetrahydro-2H-pyran-3-yl] methyl methanesulfonate (0.20 g) was dissolved with DMF (3.5 mL). To the solution, NaN3 (0.22 g, 3.4 mmol) was added and the mixture was stirred 100 °C for 3 hours. After cooling to room temperature, the mixture was diluted with ether and washed with water. The organic layer was dried over MgS04 and evaporated to give 5- (azidomethyl)-2- (phenoxymethyl) tetrahydro-2H-pyran (0.16 g).

IH NMR (CDC13) 6 : 7.32-7. 24 (m, 2H), 7.00-6. 88 (m, 3H), 4.20-3. 08 (m, 7H), 2. 08-1. 20 (m, 5H) ppm.

Preparation 46 {[6-(Phenoxymethyl)tetrahydro-2H-pyran-3-yl]methyl}amine A solution of 5- (azidomethyl)-2- (phenoxymethyl) tetrahydro-2H-pyran (0.16 g) in THF (1.0 mL) was added to a suspension of LiAlH4 (0.64 mmol) in THF (2.0 mL) at 0 °C and the mixture was stirred at room temperature for 30 min. The mixture was quenched with Na2SO4-10H20 (excess) and KF (excess). After stirring for 4 hours, the suspension was filtered and evaporated to give { [6- (phenoxymethyl) tetrahydro-2H-pyran-3- yl] methyl} amine (0.13 g).

'H NMR (CDC13) 8 : 7.32-7. 24 (m, 2H), 7.00-6. 88 (m, 3H), 4.20-2. 53 (m, 7H), 2. 08-1. 10 (m, 5H) ppm.

Preparation 47 8-r (4-Fluorobenzyl) oxyl-1, 4-dioxaspirof4. 51decane NaH (880 mg, 22 mmol; 60%) was washed with n-hexane (5 ml x 2) and the powder was dried in vacuo. To the flask was added THF (5 ml) and cooled to 0°C. To the suspension was added a solution of 1, 4-dioxaspiro [4.5] decan-8-ol (3.2 g, 20 mmol) in THF (15 ml) and the reaction mixture was stirred at room temperature for 30 min. To the mixture was added a solution of 1- (bromomethyl)-4-fluorobenzene (4.5 g, 24 mmol) in THF (5 ml) at 0°C, stirred at room temperature for 17 hr. To the reaction mixture was added NaH (400 mg, 10 mmol; 60%) and the mixture was refluxed for 6 hr. Sat. aq. NaHC03 (20 ml) was poured into the reaction mixture and the whole was extracted with ethyl acetate (50 ml x 3). The combined organic layer was dried over Na2S04, concentrated in vacuo. The residue was purified by column chromatography on silica gel (n-hexane: ethyl acetate = 10 : 1 as eluent) to afford the titled compound as yellow oil (5.0 g, 94%).

1H NMR (CD13) 6 : 7. 33-7. 27 (m, 2H), 7.04-6. 97 (m, 2H), 4. 48 (s, 2H), 3. 98-3. 89 (m, 4H), 3.54-3. 48 (m, 1H), 1.89-1. 71 (m, 6H), 1.60-1. 50 (m, 2H) ppm.

Preparation 48 4-[(4-Fluorobenzyl)oxy]cclohexanone This compound was prepared with 8- [ (4-fluorobenzyl) oxy]-1, 4-dioxaspiro [4.5] decane by a procedure similar to that in Preparation 20 as yellow oil.

1H NMR (CD3) 8 : 7.36-7. 31 (m, 2H), 7.08-7. 02 (m, 2H), 4.56 (s, 2H), 3.83-3. 81 (m, 1H), 2.67-2. 56 (m, 2H), 2.33-1. 98 (m, 6H) ppm.

Preparation 49 1-(Aminomethyl)-4-[(4-fluorobenzyl)oxy]cclohexanol This compound was prepared with 4- [ (4-fluorobenzyl) oxy] cyclohexanone by a procedure similar to that in Preparation 30 as brown oil.

'H NMR (CD3, cisltrans mixture) 6 : 7.34-7. 16 (m, 2H), 7.05-6. 99 (m, 2H), 4.53 (s, 1.4H), 4. 47 (s, 0.6H), 3.63-3. 61 (m, 0.3H), 3. 38-3. 29 (m, 0.7H), 2.64 (s, 0.6H), 2.58 (s, 1.4H), 1.90-1. 19 (m, 8H) ppm. (OH and NH2 were not observed.) MS (ESI): 254.10 (M+H) + Preparation 50 4-{[({4-[(4-Fluorobenzyl)oxy]-1-hydroxycyclohexyl}methyl)ami no]carbonyl}phenyl acetate This compound was prepared with 4-acetoxybenzoic acid and 1- (aminomethyl)-4- [ (4- fluorobenzyl) oxy] cyclohexanol by a procedure similar to that in Preparation 9 as a white solid.

'H NMR (CDC13, cisltrans mixture) S : 7.83-7. 80 (m, 2H), 7.32-7. 29 (m, 2H), 7.18-7. 15 (m, 2H), 7.08-6. 99 (m, 2H), 6.61 (br, 1H), 4.51-4. 46 (m, 2H), 3.52-3. 46 (m, 2H), 3.41-3. 39 (m, 1H), 2.35-2. 28 (m, 3H), 1. 85-1. 68 (m, 6H), 1.49-1. 41 (m, 2H) ppm. (OH was not observed.) MS (ESI): 416.03 (M+H) +, 414.03 (M-H)- Preparation 51 8-j (2-Fluorobenzvl) oxyl-1, 4-dioxaspirof4. 51decane This compound was prepared with 2-fluorobenzyl bromide by a procedure similar to that in Preparation 47.

'H NMR (CDC13) 6 : 7. 49-7.43 (m, 1H), 7. 30-7. 20 (m, 1H), 7.16-6. 97 (m, 2H), 4.59 (s, 2H), 3.97-3. 91 (m, 4H), 3.59-3. 50 (m, 1H), 1.94-1. 73 (m, 6H), 1.63-1. 48 (m, 2H) ppm.

Preparation 52 4-f (2-Fluorobenzyl) oxvlcyclohexanone This compound was prepared with 8-[(2-fluorobenzyl) oxy]-1, 4-dioxaspiro [4.5] decane by a procedure similar to that in Preparation 20 as colorless oil.

'H NMR (CDC13) 8 : 7.49-7. 43 (m, 1H), 7.33-7. 25 (m, 1H), 7.18-7. 03 (m, 2H), 4.66 (s, 2H), 3.88-3. 83 (m, 1H), 2.68-2. 58 (m, 2H), 2.32-1. 92 (m, 6H) ppm.

Preparation 53 1-(Aminomethyl)-4-[(2-fluorobenzyl)oxy]cyclohexanol This compound was prepared with 4-[(2-fluorobenzyl) oxy] cyclohexanone by a procedure similar to that in Preparation 30 as yellow oil.

'H NMR (CDCI3, cis/trans mixture) 8 : 7.49-7. 42 (m, 1H), 7.28-6. 99 (m, 3H), 4.63 (s, 1. 4H), 4.56 (s, 0.6H), 3.66-3. 65 (m, 0.4H), 3.41-3. 32 (m, 0.6H), 2.64-2. 63 (m, 0.6H), 2.58-2. 57 (m, 1. 4H), 1.92-1. 20 (m, 8H) ppm. [OH and NH2 proton were not observed.] MS (ESI) : 254.07 (M+H) + Preparation 54 4-{[({4-[(2-Fluorobenzyl)oxy]-1-hydroxycyclohexyl}methyl)ami no]carbonyl}phenyl acetate This compound was prepared with 4-acetoxybenzoic acid and 1-(aminomethyl)-4-[(2- fluorobenzyl) oxy] cyclohexanol by a procedure similar to that in Preparation 9 as a white solid.

'H NMR (CDC13, cis/trans mixture) 5 : 7.83-7. 80 (m, 2H), 7.46-7. 41 (m, 1H), 7.29-6. 99 (m, 6H), 6.55 (br, 1H), 4.62 (s, 1. 5H), 4.56 (s, 0. 5H), 3.52-3. 44 (m, 3H), 2.32 (s, 3H), 1.88-1. 66 (m, 6H), 1.51-1. 43 (m, 2H) ppm.

MS (ESI) : 416.06 (M+H) Preparation 55 8-[(3-Fluorobenzyl)oxy]-1,4-dioxaspiro[4.5]decane This compound was prepared with 3-fluorobenzyl bromide by a procedure similar to that in Preparation 47 as yellow oil.

'H NMR (CDC13) 6 : 7.33-7. 25 (m, 1H), 7.11-7. 05 (m, 2H), 6.98-6. 91 (m, 1H), 4.52 (s, 2H), 3.99-3. 91 (m, 4H), 3.55-3. 48 (m, 1H), 1.90-1. 75 (m, 6H), 1. 64-1. 50 (m, 2H) ppm.

Preparation 56 4-f (3-Fluorobenzyl) oxylcyclohexanone This compound was prepared with 8- [ (3-fluorobenzyl) oxy]-1, 4-dioxaspiro [4.5] decane by a procedure similar to that in Preparation 20 as yellow oil.

'H NMR (CDC13) b : 7.36-7. 26 (m, 1H), 7.14-7. 08 (m, 2H), 7.01-6. 95 (m, 1H), 4.60 (s, 2H), 3.83-3. 81 (m, 1H), 2.69-2. 57 (m, 2H), 2.32-2. 13 (m, 4H), 2.05-1. 98 (m, 2H) ppm.

Preparation 57 1-(Aminomethyl)-4-f (3-fluorobenzvl) oxylcyclohexanol This compound was prepared with 4- [ (3-fluorobenzyl) oxy] cyclohexanone by a procedure similar to that in Preparation 30 as yellow oil.

'H NMR (CDCI3, cis/trans mixture) 8 : 7.32-7. 24 (m, 1H), 7.11-7. 06 (m, 2H), 6.97-6. 92 (m, 1H), 4.55-4. 49 (m, 2H), 3.76-3. 31 (m, IH), 2.64-2. 56 (m, 2H), 2.02-1. 13 (m, 8H) ppm.

(OH and NH2 were not observed.) MS (ESI) : 254.11 (M+H) + Preparation 58 8-f (3-Fluorobenzyl)oxy]methyl}-1,4-dioxaspiro[4.5]decane This compound was prepared with 3-fluorobenzyl bromide and 1, 4-dioxaspiro [4.5] dec- 8-ylmethanol by a procedure similar to that in Preparation 47 as colorless oil.

IH NMR (CDCl3) 8 : 7.33-7. 26 (m, 1H), 7.10-6. 93 (m, 3H), 4.49 (s, 2H), 3.98-3. 90 (m, 4H), 3. 31 (d, J = 6.6 Hz, 2H), 1. 85-1. 50 (m, 7H), 1.35-1. 21 (m, 2H) ppm.

Preparation 59 4- (3-Fluorobenzyl) oxylmethvl} cyclohexanone This compound was prepared with 8-{[(3-fluorobenzyl)oxy]methyl}-1, 4- dioxaspiro [4.5] decane by a procedure similar to that in Preparation 20 as yellow oil.

1H NMR (CDCl3) 8 : 7.35-7. 27 (m, 1H), 7.10-6. 95 (m, 3H), 4.52 (s, 2H), 3.40 (d, J=6. 1 Hz, 2H), 2.43-2. 28 (m, 4H), 2.17-2. 06 (m, 3H), 1.55-1. 44 (m, 2) ppm.

Preparation 60 trans-1- (Aminomethyl)-4-f f (3-fluorobenzyl) oxylmethyl ? cyclohexanol This compound was prepared with 4- { [ (3-fluorobenzyl) oxy] methyl) cyclohexanone by a procedure similar to that in Preparation 30 as yellow oil.

'H NMR (DMSO-d6) 6 : 7.43-7. 40 (m, 1H), 7.16-7. 07 (m, 3H), 4. 46 (s, 2H), 3. 28 (d, J= 5.9 Hz, 2H), 2.46-2. 35 (m, 2H), 1.64-1. 61 (m, 4H), 1.27-1. 01 (m, SH) ppm. [NH2 and OH proton were not observed.] MS (ESI): 268.18 (M+H) + Preparation 61 8-f2- (2-Fluorophenoxv) ethyll 1, 4-dioxaspirof4. 51decane This compound was prepared with 2- (1, 4-dioxaspiro [4.5] dec-8-yl) ethanol and 2- fluorophenol by a procedure similar to that in Preparation 42 as colorless oil.

H NMR (CDC13) 5 : 7. 10-6. 84 (m, 4H), 4.09-4. 05 (m, 2H), 3.94 (s, 4H), 1.82-1. 74 (m, 6H), 1. 68-1. 51 (m, 3H), 1. 38-1.24 (m, 2H) ppm.

Preparation 62 4-r2-(2-Fluorophenoxy) ethyllcyclohexanone This compound was prepared with 8-[2-(2-fluorophenoxy)ethyl]1, 4- dioxaspiro [4. 5] decane by a procedure similar to that in Preparation 20 as a white solid.

IH NMR (CDC13) 8 : 7.12-6. 91 (m, 4H), 4.16-4. 05 (m, 2H), 2.41-2. 36 (m, 4H), 2.18-2. 05 (m, 3H), 1.88-1. 76 (m, 2H), 1.56-1. 42 (m, 2H) ppm.

Preparation 63 trans-1- (Aminomethyl)-2-. (2-fluorophenyl) ethyifcyclohexanol This compound was prepared with 4-[2-(2-fluorophenyl)ethyl]cyclohexanone by a procedure similar to that in Preparation 30 as a white solid.

'H NMR (DMSO-d6) 8 : 7.28-6. 86 (m, 4H), 4.15-3. 97 (m, 2H), 2.48-2. 37 (m, 2H), 1. 81-0. 94 (m, 11H) ppm. [NH2and OH proton were not observed.] MS (ESI) : 268. 11 (M+H)+ Preparation 64 Ethyl 4-hydroxycyclohexanecarboxylate To a solution of ethyl 4-oxocyclohexanecarboxylate (13.5 g, 79 mmol) in MeOH (150 mL) at 0 °C was added NaBH4 (5.3 g, 140 mmol) and the mixture was stirred at rt for 3 h.

Then the reaction was quenched by addition of H2O (50 mL) and extracted with AcOEt (150 mL x 1, 50 mL x 2). The combined organic layer was washed with H20 (50 mL), dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography (hexane-AcOEt 1.5 : 1-1 : 1) to give the titled compound (12 g, 88%, cisltrans = 3: 7) as clear oil.

IH NMR (300MHz, CDC13, cisltrans mixture) 8 : 4.17-4. 08 (m, 2H), 3.90 (bs, 0.3H), 3. 68- 3.57 (m, 0.7H), 2.42-1. 28 (m, 9H), 1.27-1. 22 (m, 3H) ppm. (OH was not observed.) Preparation 65 Ethyl 4- (4-chlorophenoxv) cvcohexanecarboxylate To a solution of ethyl 4-hydroxycyclohexanecarboxylate (3.1 g, 18 mmol) in toluene (50 mL) were added PPh3 (5.2 g, 20 mmol) and p-chlorophenol (2.6 g, 20 mmol). To the mixture was added DEAD (40% in toluene, 9.4 g, 21 mmol) at 0 °C and the mixture was stirred at rt for 7 h. The reaction was quenched by addition H20 (100 mL) and diluted with AcOEt (100 mL). The aqueous layer was extracted with AcOEt (50 mL) and the combined organic layer was washed with brine (50 mL), dried over MgS04, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography (hexane: AcOEt = 12: 1) to give the titled compound (3.5 g, 68%).

IH NMR (300MHz, CDC13, cisltrans mixture) 6 : 7.24-7. 19 (m, 2H), 6.86-6. 79 (m, 2H), 4.47-4. 40 (m, 1H), 4.18-4. 09 (m, 2H), 2.44-1. 41 (m, 9H), 1.28-1. 24 (m, 3H) ppm.

Preparation 66 [4-(4-Chlorophenoxy) cyclohexyllmethanol To a suspension of lithium aluminum hydride (1.4 g, 37 mmol) in Et20 (30 mL) was added a solution of ethyl 4- (4-chlorophenoxy) cycohexanecarboxylate (3.5 g, 12 mmol) in Et2O (30 mL) at 0 °C and the mixture was stirred at rt. After 2 h, the reaction was quenched by addition of H20 (1.4 mL), 15% NaOH (1.4 mL) and Hz0 (4.2 mL). The mixture was diluted with AcOEt (50 mL) and stirred for I h. Then the mixture was filtered and concentrated in vacuo to give the titled compound (2.9 g).

'H NMR (300MHz, CDC13, cisltrans mixture) 6 : 7.24-7. 19 (m, 2H), 6.90-6. 79 (m, 2H), 4.49-4. 05 (m, 1H), 3.53-3. 47 (m, 2H), 2.20-1. 02 (m, 9H) ppm. (OH was not observed.) Preparation 67 4-(Azidomethyl) cyclohexyl 4-chlorophenvI ether To a solution of [4- (4-chlorophenoxy) cyclohexyl] methanol (2.9 g, crude from above procedure) and Et3N (3.5 mL, 25 mmol) in CH2CI2 (100 mL) was added MsCI (1.2 mL, 15 mmol) at 0 °C. After 1.5 h, the reaction mixture was quenched by addition of sat. aq.

NaHC03 (50 mL). The aqueous layer was extracted with CH2C12 (30 mL x 2) and the combined organic layer was washed with brine (30 mL), dried over Na2S04, filtered and comcentrated in vacuo. The residue was dissolved in DMF (60 mL) and to this solution was added NaN3 (1.6 g, 25 mmol) and stirred at 80 °C for 3 h. Then the reaction was quenched by addedtion of sat. aq. NaHC03 (30 mL) and extracted with AcOEt (100 mL).

The aqueous layer was extracted with AcOEt (50 mL x 2) and the combined organic layer was extracted with H2O (50 mL x 2), brine (50 mL), dried over Na2S04, filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography (hexane: AcOEt = 1 : 15) to give the titled compound (3.0 g, 91% over 3 steps).

'H NMR (300MHz, CDC13, cisltrans mixture) 8 : 7.29-7. 18 (m, 2H), 6. 86-6. 79 (m, 2H), 4.50-4. 04 (m, 1H), 3.19 (d, J= 6.42 Hz, 2H), 2. 18-1. 10 (m, 9H).

Preparation 68 Ethyl 4- (4-fluorophenoxv) cyclohexanecarboxvlate This compound was prepared with 4-fluorophenol by a procedure similar to that in Preparation 65 as colorless oil.

'H NMR (CDCI3, cisltrans mixture) 8 : 6.98-6. 92 (m, 2H), 6.87-6. 83 (m, 2H), 4.38-4. 11 (m, 3H), 2.42-2. 28 (m, 1H), 2.18-1. 90 (m, 4H), 1.76-1. 39 (m, 4H), 1.29-1. 23 (m, 3H) ppm.

Preparation 69 f 4- (4-Fluorophenoxy) cvclohexyllmethanol This compound was prepared with ethyl 4-(4-fluorophenoxy)cyclohexanecarboxylate by a procedure similar to that in Preparation 66.

'H NMR (CD3, cisltrans mixture) # : 6. 99-6. 81 (m, 4H), 4.45-3. 70 (m, 1H), 3.54-3. 48 (m, 2H), 2. 19-1. 37 (m, 9H) ppm. (OH was not observed.) Preparation 70 1-f r4-(Azidomethvl)ccyclohexvlloxy}-4-fluorobenzene This compound was prepared with [4- (4-fluorophenoxy) cyclohexyl] methanol by a procedure similar to that in Preparation 67.

'H NMR (CDC13, cisltrans mixture) 6 : 6.99-6. 81 (m, 4H), 4.45 (br, 1H), 3.21-3. 18 (m, 2H), 2.19-1. 41 (m, 9H) ppm.

Preparation 71 ff4- (4-Fluorophenoxv) cvclohexvll methyl amine To a solution of 1- { [4- (azidomethyl) cyclohexyl] oxy}-4-fluorobenzene (5. 6 g, 21 mmol) in MeOH (50 ml) was added 10% Pd-C (0.5 mg) and the whole mixture was stirred at room temperature for 5 hr under hydrogen atmosphere. The reaction mixture was filtered through a pad of celite and the filtrate was concentrated in vacuo to afford the titled compound as colorless oil (4.4 g).

'H NMR (DMSO-d6, cis/trans mixture) 6 : 8. 05 (br, 2H), 7.14-7. 06 (m, 2H), 6. 98-6. 93 (m, 2H), 4.52 (br, 0.8H), 4.19 (br, 0.2H), 2.81 (d, J = 6.6 Hz, 0.4H), 2.69 (d, J = 6.8 Hz, 1.6H), 2.05-1. 08 (m, 9H) ppm.

MS (ESI) : 224.11 (M+H)+ Preparation 72 N {[cis-4-(4-Fluorophenoxy)cyclohexyl]metyl}-4-(methoxymethoxy )benzamide This compound was prepared with { [4- (4-fluorophenoxy) cyclohexyl] methyl} amine by a procedure similar to that in Preparation 9 as a white solid.

IH NMR (DMSO-d6) 8 : 8.34 (m, 1H), 7.83-7. 80 (m, 2H), 7.12-7. 04 (m, 4H), 6.98-6. 93 (m, 2H), 5.24 (s, 2H), 4.50 (br, 1H), 3.38 (s, 3H), 3.18-3. 13 (m, 2H), 1.88-1. 31 (m, 9H) ppm.

Preparation 73 Ethyl cis-4- cvclohexanecarboxelate Iodotrimethylsilane (0.36 mL, 2.5 mmol) was added to a mixture of ethyl 4- oxocyclohexanecarboxylate (8.5 g, 50 mmol) and dimethyl (2-phenylethoxy) silane (9.9 g, 55 mmol) (Synlett 2002,313-315.) in CH2CI2 (50 mL) at 0 °C and the mixture was stirred at room temperature for 16 hours. The mixture was quenched with water and extracted with AcOEt (200 mL). The extract was washed with sat. aq. NaHC03 (50 mL), dried over MgS04 and concentrated. The residue was purified by silica gel column chromatography (hexane-AcOEt 15: 1) to give the titled compound (2.7 g).

IH NMR (CDCl3) 8 : 7.35-7. 15 (m, 5H), 4.13 (q, J= 7.1 Hz, 2H), 3.60 (t, J= 7.3 Hz, 2H), 3.52-3. 40 (m, 1H), 2.87 (t, J = 7.3 Hz, 2H), 2.42-2. 24 (m, 1H), 1.97-1. 40 (m, 8H), 1.25 (t, J = 7. 1 Hz, 3H) ppm.

Preparation 74 [cis-4-(2-Phenylethoxy)cyclohexyl]methanol This compound was prepared ethyl cis-4- (2-phenylethoxy) cyclohexanecarboxylate by a procedure similar to that in Preparation 66.

1H NMR (CD3) 8 : 7.40-7. 12 (m, SH), 3.81-3. 39 (m, 5H), 2.87 (t, J= 7.1 Hz, 2H), 1.95- 1.15 (m, 9H) ppm. (OH was not observed.) Preparation 75 cis-4- (Azidomethvl) cvclohexvl 2-phenylethyl ether This compound was prepared [cis-4- (2-phenylethoxy) cyclohexyl] methanol by a procedure similar to that in Preparation 67.

'H NMR (CDCI3) b : 7.37-7. 12 (m, 5H), 3.66-3. 48 (m, 3H), 3.00 (d, J = 6.8 Hz, 2H), 2.87 (t, J= 7.1 Hz, 2H), 1. 96-1. 17 (m, 9H) ppm.

Preparation 76 { lcis-4- (2-Phenvlethoxv) cyclohexyllmethvllamine This compound was prepared cis-4- (azidomethyl) cyclohexyl 2-phenylethyl ether by a procedure similar to that in Preparation 71.

'H NMR (CDC13) 8 : 7.40-7. 14 (m, 5H), 3.72-3. 46 (m, 3H), 2.87 (t, J = 7.3 Hz, 2H), 2.53 (d, J = 5.4 Hz, 2H), 2.00-1. 15 (m, 9H) ppm. (NHz was not observed.) Preparation 77 Benzyl (fcis-4- (hydroxymethyl) clohexyl]methyl}carbamate Benzylchloroformate (15 mL, 0. 11 mol) was added dropwise to a mixture of [cis-4- (aminomethyl) cyclohexyl] methanol (14 g, 0.10 mol) and diisopropylethylamine (21 mL, 0.12 mol) in CH2CI2 (200 mL) at 0 °C and the mixture was stirred at room temperature for 2 hours. The mixture was diluted with CH2C12 (200 mL) and washed with sat. aq. NH4CI. The organic layer was dried over MgSO4 and concentrated in vacuum. The residue was purified by silica gel column chromatography (hexane-AcOEt 1: 1 to 1: 2) to give the titled compound (14 g).

'H NMR (CDCl3)# : 7.38-7. 26 (m, 5H), 5.10 (s, 2H), 4. 80-4. 70 (m, 1H), 3.58-3. 50 (m, 2H), 3.20-3. 10 (m, 2H), 1.75-1. 20 (m, 10H) ppm. (OH was not observed.) Preparation 78 Benzyl ( {cis-4-[(benzyloxy)methyl]cyclohexyl}methyl)carbamate NaH (88 mg, 2.2 mmol) was added to a solution of benzyl { [cis-4- (hydroxymethyl) cyclohexyl] methyl} carbamate (0.55 g, 2.0 mmol) in THF (4.0 mL) at 0 °C and the mixture was stirred at room temperature for 30 min. Benzylbromide (0.29 mL, 2.4 mmol) was added to the mixture at 0 °C. The mixture was sitrred at room temperature for 7 hours. The mixture was quenched with sat. aq. NH4CI and extracted with CH2Cl2. The extract was dried over MgS04 and concentrated in vacuum. The residue was purified by silica gel column chlomatography (hexane-AcOEt 8: 1) to give the titled compound (0.27 g). tH NMR (CDCl3) 6 : 7.40-7. 24 (m, 10H), 5.09 (s, 2H), 4. 80-4. 65 (m, 1H), 4.49 (s, 2H), 3.36 (d, J = 7.1 Hz, 2H), 3.20-3. 08 (m, 2H), 1.90-1. 22 (m, 10H) ppm.

Preparation 79 ({cis-4-[(Benzyloxy)methyl]cyclohexyl}methyl) amine A mixture of benzyl ({cis-4-[(benzyloxy) methyl] cyclohexyl} methyl) carbamate (0.27 g, 0.73 mmol) and KOH (0.21 g, 3.7 mmol) in EtOH (0.40 mL) was refluxed for 3 hours. The mixture was acidified with 2N aq. HCl (20 mL) and the aqueous layer was washed with AcOEt. The aqueous layer was alkalized with 2N aq. NaOH (21 mL) and extracted with CH2C12. The extract was dried over MgS04 and concentrated to give the titled compound (0.10 g).

'H NMR (CDCl3) S : 7.40-7. 25 (m, 5H), 4.50 (s, 2H), 3. 37 (d, J = 7.1 Hz, 2H), 2.60 (d, J = 6.6 Hz, 2H), 1.94-1. 80 (m, 1H), 1.64-1. 22 (m, 9H) ppm.. (NH2 was not observed.) Preparation 80 8-(2-Phenoxyethyl)-1 4-dioxaspirof4. 51decane This compound was prepared with 2- (1, 4-dioxaspiro [4.5] dec-8-yl) ethanol by a procedure similar to that in Preparation 42.

'H NMR (CDCl3) # : 7. 31-7. 24 (m, 2H), 6.96-6. 86 (m, 3H), 3.99 (t, J = 6.4 Hz, 2H), 3.95 (s, 4H), 1.84-1. 22 (m, l lH) ppm.

Preparation 81 4-(2-Phenoxyethel) cyclohexanone This compound was prepared with 8-(2-phenoxyethyl)-1, 4-dioxaspiro [4. 5] decane by a procedure similar to that in Preparation 20.

'H NMR (CDCl3) # : 7.34-7. 25 (m, 2H), 7.00-6. 88 (m, 3H), 4.05 (t, J = 6.3 Hz, 2H), 2.45- 1.40 (m, I I H) ppm.

Preparation 82 trans-1-(Aminomethyl)-4-(2-phenoxyethyl)cyclohexanol hydrochloride This compound was prepared with 4- (2-phenoxyethyl) cyclohexanone by a procedure similar to that in Preparation 16.

'H NMR (DMSO-d6) 8 : 7.75 (br, 3H), 7.32-7. 23 (m, 2H), 6.97-6. 88 (m, 3H), 4.99 (br, 1H), 3. 98 (t, J= 6.4 Hz, 2H), 2.82 (s, 2H), 1. 78-1. 00 (m, 11H) ppm.

Preparation 83 8-{[(4-Fluorobenzyl)oxy]methyl}-1,4-dioxaspiro[4,5]decane This compound was prepared with 4-fluorobenzyl bromide and 1, 4-dioxaspiro [4. 5] dec- 8-ylmethanol by a procedure similar to that in Preparation.

'H NMR (CDCl3) 8 : 7.40-7. 24 (m, 2H), 7.10-6. 98 (m, 2H), 4.45 (s, 2H), 3.94 (s, 4H), 3.30 (d, J = 6.6 Hz, 2H), 1. 86-1. 20 (m, 9H) ppm.

Preparation 84 4-{[4-Fluorobenzyl)oxy]methyl}cyclohexanone This compound was prepared with 8-{[(4-fluorobenzyl)oxy]methyl}-1, 4- dioxaspiro [4.5] decane by a procedure similar to that in Preparation 20.

'H NMR (CDCI3) 8 : 7.35-7. 25 (m, 2H), 7.10-7. 00 (m, 2H), 4.48 (s, 2H), 3.38 (d, J = 6.1 Hz, 2H), 2.48-2. 00 (m, 7H), 1.56-1. 36 (m, 2H) ppm.

Preparation 85 trans-1-(Aminomethyl)-4-{[(4-fluorobenzyl)oxy]methyl}cyclohe xanol hydrochloride This compound was prepared with 4- { [ (4-fluorobenzyl) oxy] methyl}cyclohexanone by a procedure similar to that in Preparation 16.

'H NMR (DMSO-d6) 6 : 7.86 (br, 3H), 7.39-7. 31 (m, 2H), 7.22-7. 13 (m, 2H), 5.04 (br, 1H), 4.42 (s, 2H), 3.28 (d, J = 6.2 Hz, 2H), 2.79 (s, 2H), 1. 75-1. 00 (m, 9H) ppm.

Preparation 86 8-f (2-Fluorophenoxy) methyl]-1,4-dioxaspiro[4.5]decane This compound was prepared with 2- (1, 4-dioxaspiro [4.5] dec-8-yl) methanol and 2- fluorophenol by a procedure similar to that in Preparation 42.

1H NMR (CDCI3) 6 : 7.12-6. 80 (m, 4H), 3.96 (s, 4H), 3.86 (d, J= 6.3 Hz, 2H), 2.00-1. 28 (m, 9H) ppm.

Preparation 87 4-r (2-Fluorophenoxv) methyllcyclohexanone This compound was prepared with 8-[(2-fluorophenoxy)methyl]-1, 4- dioxaspiro [4.5] decane by a procedure similar to that in Preparation 20.

'H NMR (CDC13) 8 : 7.13-6. 80 (m, 4H), 3.94 (d, J = 6. 2 Hz, 2H), 2.50-2. 16 (m, 7H), 1.68- 1.50 (m, 2H) ppm.

Preparation 88 trans-1- (Aminomethyl)-4-f (2-fluorophenoxy) methyllcyclohexanol hydrochloride This compound was prepared with 4-[(2-fluorophenoxy)methyl] cyclohexanone by a procedure similar to that in Preparation 16.

'H NMR (DMSO-d6) 5 : 7.87 (br, 3H), 7.24-7. 07 (m, 3H), 6.96-6. 88 (m, 1H), 5.08 (s, 1H), 3.92 (d, J = 6.4 Hz, 2H), 2. 83 (s, 2H), 1.90-1. 12 (m, 9H) ppm.

Preparation 89 8-r 4-Fluorophenoxy) methyl1-1, 4-dioxaspirof4. 51decane This compound was prepared with 2- (1, 4-dioxaspiro [4.5] dec-8-yl) methanol and 4- fluorophenol by a procedure similar to that in Preparation 42.

'H NMR (CDCI3) b : 7.05-6. 70 (m, 4H), 3.96 (s, 4H), 3.75 (d, J = 6.2 Hz, 2H), 1.96-1. 20 (m, 9H) ppm.

Preparation 90 4-1 (4-Fluorophenoxy) methyllcyclohexanone This compound was prepared with 8- [ (4-fluorophenoxy) methyl]-1, 4- dioxaspiro [4.5] decane by a procedure similar to that in Preparation 20.

'H NMR (CDCI3) 5 : 7.12-6. 72 (m, 4H), 3.84 (d, J= 5.9 Hz, 2H), 2.58-2. 12 (m, 7H), 1.70- 1.48 (m, 2H) ppm.

Preparation 91 trans-l-(Aminomethyl)-4-[(4-fluorophenoxy) methYllcYclohexanol hydrochloride This compound was prepared with 4- [ (4-fluorophenoxy) methyl] cyclohexanone by a procedure similar to that in Preparation 16.

'H NMR (DMSO-d6) 8 : 7.83 (br, 3H), 7.18-7. 05 (m, 2H), 7.00-6. 88 (m, 2H), 5.07 (s, 1H), 3.81 (d, J= 6.2 Hz, 2H), 2.83 (s, 2H), 1.87-1. 10 (m, 9H) ppm.

Preparation 92 N-[(trans-1-Hydroxy-4-{[(5-methylpyridin-2-yl)oxy]methyl}cyc lohexyl)methyl}-4- (methoxymethoxy) benzamide A mixture of N-{[trans-1-hydroxy-4-(hydroxymethyl) cyclohexyl] methyl}-4- (methoxymethoxy) benzamide (0. 16g, 0.50 mmol) and NaH (60%, 24 mg, 0.60 mmol) was stirred at room temperature for 30 min. 2-Fluoro-5-methylpyridine (78 mg, 0.70 mmol) was added to the mixture. The mixture was stirred at 200 °C for 10 min with microwave irradiation, and quenched with NaHC03. The whole was extracted with AcOEt. The extract was washed with water, dried over MgSO4 and concentrated. The residue was purified by silica gel column chromatography (hexane: AcOEt = 4: 5) to give the titled compound (97 mg).

'H NMR (CDCI3) 8 : 7.90-7. 80 (m, 1H), 7.76 (d, J= 8.9 Hz, 2H), 7.43-7. 33 (m, 1H), 7.07 (d, J = 8.9 Hz, 2H), 6.64 (d, J = 8.2 Hz, 1H), 6. 58-6. 47 (m, 1H), 5.22 (s, 2H), 4.13 (d, J = 6.3 Hz, 2H), 3.60 (d, J = 5.9 Hz, 2H), 3.48 (s, 3H), 2.57 (br, 1H), 2.24 (s, 3H), 2.00-1. 77 (m, 5H), 1.60-1. 21 (m, 4H) ppm.

Preparation 93 8- (Aminomethyl)-1, 4-dioxaspirof4. 51decan-8-ol This compound was prepared with 8-oxo-1, 4-dioxaspiro [4. 5] decane by a procedure similar to that in Preparation 30.

'H NMR (CDCl3) 8 : 3.98-3. 90 (m, 4H), 2.97 (br, 1H), 2.02-1. 45 (m, 8H) ppm. (NH2 was not observed.) Preparation 94 4-(BenzeloxY)-N-r (8-hydroxy-1 4-dioxaspiroF4. 51dec-8-yl) methyllbenzamide This compound was prepared with 4-benzyloxybenzoic acid and 8-(aminomethyl)-1, 4- dioxaspiro [4.5] decan-8-ol by a procedure similar to that in Preparation 9.

'H NMR (CDC13) 8 : 7.75 (d, J = 8.9 Hz, 2H), 7.50-7. 30 (m, 5H), 6.98 (d, J = 8. 9 Hz, 2H), 6.63-6. 53 (m, 1H), 5.10 (s, 2H), 3.96-3. 92 (m, 4H), 3.49 (d, J= 5.9 Hz, 2H), 2.96 (br, 1H), 1.96-1. 56 (m, 8H) ppm.

Preparation 95 4- (Benzyloxv)-N-f (l-hydroxy-4-oxocyclohexyl) methyllbenzamide This compound was prepared with 4- (benzyloxy)-N- [ (8-hydroxy-1, 4-dioxaspiro [4.5] dec- 8-yl) methyl] benzamide by a procedure similar to that in Preparation 20.

'H NMR (CDCI3) 6 : 7.76 (d, J = 8.9 Hz, 2H), 7.46-7. 30 (m, 5H), 7.02 (d, J = 8. 9 Hz, 2H), 6.60-6. 50 (m, 1H), 5.12 (s, 2H), 3.86 (s, 1H), 3.37 (d, J = 5.9 Hz, 2H), 2.84-2. 68 (m, 2H), 2.36-2. 24 (m, 2H), 2.14-2. 00 (m, 2H), 1.85-1. 70 (m, 2H) ppm.

Preparation 96 N-r (4-Benzylidene-l-hydroxycyclohexyl) methyll-4- (benzyloxy) benzamide A mixture of 4- (benzyloxy)-N-[ (1-hydroxy-4-oxocyclohexyl) methyl] benzamide (0.35 g, 1.0 mmol), diethyl benzylphosphonate (0.46 g, 2.0 mmol) and NaH (60%, 0.16 g, 4.0 mmol) in Dimethoxyethane (10 mL) was stirred at room temperature for 16 hours. The mixture was quenched with water and extracted with AcOEt. The extract was washed with sat. aq. NaCI, dried over MgSO4 and concentrated. The residue was purified by silica gel column chromatography (hexane: AcOEt = 3: 2) to give the titled compound (42 mg).

IH NMR (CDC13) 8 : 7.87 (d, J = 8.6 Hz, 2H), 7.50-7. 13 (m, 10H), 7.01 (d, J= 8. 6 Hz, 2H), 6.54-6. 51 (m, 1H), 6.31 (s, 1H), 5.12 (s, 2H), 3.57-3. 50 (m, 2H), 2.71-2. 23 (m, 5H), 1.90- 1.45 (m, 3H) ppm. (OH was not observed.) Preparation 97 8-{[(2-Fluorobenzyl)oxy]methyl}-1,4-dioxaspiro[4.5]decane This compound was prepared with 2-fluorobenzyl bromide and 1, 4-dioxaspiro [4.5] dec- 8-ylmethanol by a procedure similar to that in Preparation 47.

'H NMR (CDCI3) 6 : 7.46-6. 98 (m, 4H), 4.56 (s, 2H), 3.94 (s, 4H), 3.34 (d, J = 6.6 Hz, 2H), 1.90-1. 20 (m, 9H) ppm.

Preparation 98 4-f r (2-Fluorobenzyl) oxylmethylTcyclohexanone This compound was prepared with 8-{[(2-fluorobenzyl)oxy]methyl}-1, 4- dioxaspiro [4. 5] decane by a procedure similar to that in Preparation 20.

IH-NMR (CDCI3) 8 : 7.45-7. 37 (m, IH), 7.34-7. 23 (m, 1H), 7.17-7. 02 (m, 2H), 4.59 (s, 2H), 3.43 (d, J= 6. 0Hz, 2H), 2.45-2. 27 (m, 4H), 2.18-2. 05 (m, 3H), 1. 54-1. 38 (m, 2H) ppm.

Preparation 99 trans-1-(Aminomethyl)-4-{[(2-fluorobenzyl)oxy]methyl}cyclohe xanol This compound was prepared with 4- { [ (2-fluorobenzyl) oxy] methyl} cyclohexanone by a procedure similar to that in Preparation 30 as yellow oil.

'H-NMR (CDCl3) 6 : 7. 89 (br, 2H), 7.38 (dd, J = 7.5, 7.3 Hz, 1H), 7.27-7. 22 (m, 1H), 7.12 (dd, J = 7.5, 7.3Hz, 1H), 7.02 (dd, J = 9, 8. 4Hz, 1H), 4.53 (s, 2H), 3.31 (s, 2H), 3.15 (s, 2H), 1.90-1. 45 (m, 7H), 1.20-0. 98 (m, 2H) ppm. (OH was not observed.) Preparation 100 f [4- (Nitromethvl) cyclohex-3-en-1-vllmethoxv, benzene A mixture of 4-(phenoxymthyl) cyclohexanone (3. 1 g, 15 mmol) and ethylene diamine (0.10 mL, 1.5 mmol) in nitromethane (60 mL) was refluxed for 6 hours. The mixture was concentrated and purified by silica gel column chromatography (hexane: AcOEt = 10: 1) to give the titled compound (3.2 g).

'H NMR (CD3) 8 : 7.32-7. 24 (m, 2H), 6.98-6. 87 (m, 3H), 6.00-5. 93 (m, 1H), 4. 84 (s, 2H), 3. 86 (d, J = 6. 2 Hz, 2H), 2.44-1. 94 (m, 6H), 1. 58-1. 45 (m, 1H) ppm.

Preparation 101 f (4- (Phenoxvmethvl) cvclohexvllmethvl amine hydrochloride NaBH4 (2.2 g, 59 mmol) was added to a mixture of { [4-(nitromethyl) cyclohex-3-en-1- yl] methoxy} benzene (3.2 g, 13 mmol) and NiC12-6H2O in MeOH (130 mL) and THF (65 mL) at 0 °C and the mixture was stirred for 2 hours. The mixture was absorbed to amine-gel (10 g) and evaporated. The residue was eluted with CHxCIz-MeOH (10: 1). After evaporation, a mixture of the residue and 10% Pd-C (1.0 g) in EtOH (100 mL) was hydrogenated at 1 atm for 3 hours. The mixture was filtered through a pad of celite and the filtrate was concentrated. To a solution of the residue in AcOEt (20 mL), 4N HCl in AcOEt (3 mL) was added and collected with filtration to give the titled compound (1.9 g).

1H NMR (DMSO-d6) # : 8.03 (br, 3H), 7.32-7. 24 (m, 2H), 6.96-6. 88 (m, 3H), 3.90-3. 75 (m, 2H), 2.80-2. 60 (m, 2H), 1.97-0. 90 (m, I OH) ppm.

Preparation 102 Methyl cis-4-f (dibenzylamino) carbonyllcyclohexanecarboxylate This compound was prepared with dibenzylamine by a procedure similar to that in Preparation 1 as yellow oil.

'H NMR (CDCl3) 8 : 7.40-7. 23 (m, 6H), 7.18-7. 13 (m, 4H), 4.57 (s, 2H), 4.47 (s, 2H), 3.72 (s, 3H), 2.63-2. 54 (m, 2H), 2.30-2. 23 (m, 2H), 1.92-1. 78 (m, 2H), 1.71-1. 64 (m, 2H), 1.57- 1. 44 (m, 2H) ppm.

Preparation 103 f cis-4-f (Dibenzvlamino) methyllcyclohexvl) methanol To a suspension of LiAIH4 (2.1 g, 55 mmol) in THF (100 mL) was added a solution of methyl cis-4-[(dibenzylamino) carbonyl] cyclohexanecarboxylate (8.0 g, 22 mmol) in THF (100 mL) at 0°C, and the mixture was refluxed for 3 hr. The mixture was stirred at 70°C for 16hr. To the reaction mixture were added Na2S04'10H20 (20 g) and KF (2.0 g) and the mixture was stirred at room temperature for 1 hr. The mixture was filtered through a pad of celite and the filtrate was evaporated in vacuo to afford the titled compound as colorless oil (8. 4 g). tu NMR (CDCl3) 8 : 7.37-7. 21 (m, 10H), 3.77-3. 66 (m, 1H), 3.50 (s, 4H), 3.34 (d, J= 6.8 Hz, 2H), 2.27 (d, J= 7.5 Hz, 2H), 1.95-1. 31 (m, 8H), 1. 06-0. 94 (m, 2H) ppm.

Preparation 104 Dibenzyl {[cis-4-(phenoxymethyl)cyclohexyl]methyl}amine To a solution of {cis-4-[(dibenzylamino) methyl] cyclohexyl}methanol (3.0 g, 9.3 mmol) in toluene (30 mL) were added triphenylphosphine (2.7 g, 10 mmol) and phenol (1.0 g, 10 mmol). After cooling to 0°C, to the mixture was added DIAD (2.0 mL, 10.2 mmol), and the mixture was stirred at room temperature for 3.5 hr. The solvent was removed in vacuo.

The residue was purified by column chromatography on silica gel (hexane: ethyl acetate = 10: 1 as eluent) to afford the titled compound as a white solid (3.5 g, 94%).

'H NMR (CDC13) 8 : 7.38-7. 19 (m, 12H), 6.94-6. 83 (m, 3H), 3.67 (d, J= 6.8 Hz, 2H), 3.52 (s, 4H), 2.30 (d, J = 7. 6 Hz, 2H), 1.87-1. 18 (m, 10H) ppm.

Preparation 105 f [cs-4-(PhenoxYmethyl) cyclohexyllmethyl lamine hvdrochloride To a solution of dibenzyl{[cis-4-(phenoxymethyl)cyclohexyl]methyl}amine (3.5 g, 8.8 mmol) in MeOH (150 mL) was added 20% Pd (OH) 2-C (1.8 g) and the mixture was hydrogenated under 4 atm at 50°C for 13 hr. To the reaction mixture was added 10%HCl- MeOH (10 mL) and the mixture was hydrogenated under 4 atm at 55°C for 10 hr. The reaction mixture was filtered through a pad of celite and the filtrate was concentrated in vacuo. To a solution of the residue in MeOH (60 mL) were added 10% HCl-MeOH (10 mL) and 10% Pd-C (0.9 g). The mixture was hydrogenated under 4 atm at 55°C for 15 hr. The reaction mixture was filtered through a pad of celite and the filtrate was concentrated in vacuo. To a mixture of the crude material in AcOEt (15 mL) was added 4N HCI-AcOEt (2.2 mL) and the mixture was stirred at room temperature for 4 hr. The precipitate was filtered, washed with AcOEt, and dried in vacuo to afford the titled compound as a white solid (820 mg, 37%).

'H NMR (DMSO-d6) 5 : 7.95-7. 84 (m, 3H), 7.31-7. 25 (m, 2H), 6.94-6. 89 (m, 3H), 3.88 (d, J = 7.0 Hz, 2H), 2.76 (d, J = 7.1 Hz, 2H), 1.92-1. 80 (m, 2H), 1.52-1. 47 (m, 8H) ppm.

Preparation 106 (2S)-2- ( r (4-Methvlphenvl) sulfonylloxylmethvl) hex-5-en-1-yl acetate To a mixture of (2R)-2- (hydroxymethyl) hex-5-en-1-yl acetate (7.9 g, 46 mmol) (TetrahedronAsymmery 1999, 10, 4057-4064. ) and triethylamine (19 mL, 0.14 mol) in CH2CI2 (90 mL), p-TsCI (13 g, 49 mmol) was added at 0 °C and the mixture was stirred at room temperature for 7 h. The mixture was washed with sat. aq. NaCI, dried over MgS04 and evaporated. The residue was purified by silica gel column chlomatography (hexane: AcOEt = 8: 1 to 6: 1) to give the titled compound (13 g).

'H NMR (CDC13) 6 : 7.79 (d, J = 8.3 Hz, 2H), 7.36 (d, J = 8. 3 Hz, 2H), 5.79-5. 62 (m, 1H), 5.03-4. 92 (m, 2H), 4.08-3. 89 (m, 4H), 2.46 (s, 3H), 2.09-1. 92 (m, 6H), 1.51-1. 35 (m, 2H) ppm.

Preparation 107 (2S)-2-({[(4-Methylphenyl)sulfonyl]oxy}methyl)-4-oxiran-2-yl butyl acetate This compound was prepared with (2S)-2-({ [(4-methylphenyl) sulfonyl] oxy} methyl) hex- 5-en-1-yl acetate by a procedure similar to that in Preparation 40.

'H NMR (CDC13) b : 7. 80 (d, J = 8.2 Hz, 2H), 7.36 (d, J = 7.9 Hz, 2H), 4.20-3. 87 (m, 4H), 2. 91-2. 39 (m, 6H), 2.18-1. 32 (m, 8H) ppm.

Preparation 108 (2S)-2- (Hvdroxymethyl)-4-oxiran-2-ylbutyl 4-methylbenzenesulfonate To a solution of (2S)-2-({ [(4-methylphenyl) sulfonyl] oxy} methyl)-4-oxiran-2-ylbutyl acetate (14 g, 0.37 mmol) in MeOH (200 mL), K2CO3 (10 g, 74 mmol) was added and stirred at 0 °C for 30 min. The mixture was filtered and The filtrate was diluted with AcOEt.

It was washed with water, dried over MgS04 and evaporated to give the titled compound (12 g).

1H NMR (CDC13) 8 : 7.80 (d, J = 8. 2 Hz, 2H), 7.36 (d, J = 8.1 Hz, 2H), 4.19-3. 99 (m, 2H), 3.71-3. 53 (m, 2H), 2.91-2. 82 (m, 1H), 2.78-2. 71 (m, 1H), 2.54-2. 42 (m, 4H), 2.13-1. 12 (m, 5H) ppm. (OH was not observed.) Preparation 109 [ (3S)-6- (Hydroxymethyl) tetrahydro-2H-pvran-3-vllmethyl 4-methylbenzenesulfonate To a solution of (2S)-2-(hydroxymethyl)-4-oxiran-2-ylbutyl 4-methylbenzenesulfonate (10 g, 31 mmol) in CH2CI2 (100 mL), p-TsOH-H2O (0.29 g, 1.5 mmol) was added at 0 °C and the mixture was stirred at 0 °C for 1 hour and at room temperature for 30 min. The mixture was washed with sat. aq. NaHC03, dried over MgS04 and evaporated. The residue was purified by silica gel column chlomatography (hexane: AcOEt = 3: 2 to 2: 3) to give the titled compound (6.1 g).

'H NMR (CDCl3)# : 7.87-7. 72 (m, 2H), 7.44-7. 31 (m, 2H), 4. 24-3. 05 (m, 7H), 2.46 (s, 3H), 2.10-1. 13 (m, 5H) ppm. (OH was not observed.) Preparation 110 f (3S)-6-r (4-Fluorophenoxy) methylltetrahydro-2H-pyran-3-yl} methyl 4-<BR> <BR> methylbenzenesulfonate This compound was prepared with [ (3S)-6- (hydroxymethyl) tetrahydro-2H-pyran-3- yl] methyl 4-methylbenzenesulfonate by a procedure similar to that in Preparation 106.

'H NMR (CDC13) 8 : 7. 85-7. 75 (m, 2H), 7.41-7. 32 (m, 2H), 7.04-6. 72 (m, 4H), 4. 30-3. 10 (m, 7H), 2.55-2. 40 (m, 3H), 2.16-1. 18 (m, SH) ppm.

Preparation 111 (5R)-5- (Azidomethyl)-2-f (4-fluorophenoxy) methylltetrahydro-2H-pyran This compound was prepared with {(3S)-6-[(4-fluorophenoxy) methyl] tetrahydro-2H- pyran-3-yl} methyl 4-methylbenzenesulfonate by a procedure similar to that in Preparation 7.

'H NMR (CDC13) 8 : 7.05-6. 75 (m, 4H), 4.18-3. 05 (m, 7H), 2.11-1. 17 (m, 5H) ppm.

Preparation 112 ({(3R)-6-[(4-Fluorophenoxy)methyl]tetrahydro-2H-pyran-3-yl}m ethyl)amine This compound was prepared with (5R)-5- (azidomethyl)-2- [ (4- fluorophenoxy) methyl] tetrahydro-2H-pyran by a procedure similar to that in Preparation 8.

'H NMR (CDC13) 6 : 7.03-6. 81 (m, 4H), 4.18-2. 50 (m, 7H), 2. 15-1. 11 (m, 5H) ppm. (NH2 was not observed.) Preparation 113 2-f (4-Fluorophenoxy) methyllhex-5-en-1-ol This compound was prepared with 2-but-3-en-1-ylpropane-1, 3-diol by a procedure similar to that in Preparation 104.

'H NMR (CDCl3) 6 : 7.05-6. 85 (m, 4H), 5.90-5. 73 (m, 1H), 5.10-4. 95 (m, 2H), 4.05-3. 90 (m, 2H), 3.83-3. 68 (m, 2H), 2.25-1. 98 (m, 3H), 1.93-1. 78 (m, 1H), 1.67-1. 48 (m, 2H). (OH was not observed.) Preparation 114 2-f (4-Fluorophenoxv) methyll-4-oxiran-2-ylbutan-1-ol This compound was prepared with 2- [ (4-fluorophenoxy) methyl] hex-5-en-l-ol by a procedure similar to that in Preparation 40.

H NMR (CDCI3) b : 7.04-6. 74 (m, 4H), 4.05-3. 90 (m, 2H), 3.85-3. 69 (m, 2H), 30.1-2. 90 (m, 1H), 2.81-2. 73 (m, 1H), 2.55-2. 47 (m, 1H), 2.11-1. 48 (m, 5H) ppm. (OH was not observed.) Preparation 115 f (2S*,5S*)-5-[(4-Fluorophenoxy)methyl]tetrahydro-2H-pyran-2-y l}methanol This compound was prepared with 2- [ (4-fluorophenoxy) methyl]-4-oxiran-2-ylbutan-1-ol by a procedure similar to that in Preparation 109.

'H NMR (CDCI3) 5 : 7. 03-6. 75 (m, 4H), 4.15-4. 05 (m, 2H), 4.00-3. 91 (m, 1H), 3.70-3. 45 (m, 4H), 2.05-1. 75 (m, 3H), 1.55-1. 34 (m, 2H) ppm. (OH was not observed.) Preparation 116 (2S*. SS*)-2- (Azidomethvl)-5- [ (4-fluorophenoxY) methylltetrahydro-2H-pyran This compound was prepared with {(2S*, 5S*)-5-[(4-fluorophenoxy) methyl] tetrahydro- 2H-pyran-2-ylmethanol by a procedure similar to that in Preparation 67.

'H NMR (CDC13) 8 : 7.05-6. 75 (m, 4H), 4.18-3. 95 (m, 3H), 3.70-3. 46 (m, 2H), 3.34-3. 15 (m, 2H), 2.05-1. 75 (m, 3H), 1.55-1. 40 (m, 2H) ppm.

Preparation 117 ({(2S*,5S*)-5-[(4-Fluorophenoxy)methyl]tetrahydro-2H-pyran-2 -yl}methyl)amine This compound was prepared with (2S*, 5S*)-2- (azidomethyl)-5- [ (4- fluorophenoxy) methyl] tetrahydro-2H-pyran by a procedure similar to that in Preparation 8.

'H NMR (CDCI3) 8 : 7.03-6. 81 (m, 4H), 4.20-3. 95 (m, 2H), 3.70-3. 60 (m, 1H), 3.42-3. 20 (m, 2H), 2.73-2. 64 (m, 2H), 2.15-1. 70 (m, 3H), 1.56-1. 25 (m, 2H) ppm. (NH2 was not observed.) Preparation 118 {cis-4-[(Dibenzylamino)methyl]cyclohexyl}methyl methanesulfonate To a solution of {cis-4-[(dibenzylamino) methyl] cyclohexyl} methanol (35 g, 108 mmol) in dichloromethane (200 mL) was added triethylamine (30 mL, 216 mmol). To the mixture was added methanesulfonyl chloride (10 mL, 130 mmol) at 0°C, and the mixture was stirred at 0°C for 1 hr. To the mixture was added sat. aq. NaHC03 (250 mL), and the whole mixture was extracted with dichloromethane (100 mL X 3). The combined organic layer was washed with brine (300 mL), dried over Na2SO4, concentrated in vacuo to afford the titled compound as yellow oil (46 g).

'H NMR (CDCI3) 8 : 7.37-7. 19 (m, 10H), 3.91 (d, J = 7.1 Hz, 2H), 3.50 (s, 4H), 2.93 (s, 3H), 2. 28 (d, J = 7.6 Hz, 2H), 1.91-0. 98 (m, 10 H) ppm.

Preparation 119 f cis-4-f (Dibenzvlamino) methvllcyclohexyl acetonitrile To a solution of {cis-4-[(dibenzylamino) methyl] cyclohexyl}methyl methanesulfonate (46 g, 108 mmol) in DMSO (200 mL) were added sodium cyanide (8. 0 g, 162 mmol) and 15-crown-5 ether (11 mL, 54 mmol) and the reaction mixture was stirred at 60°C for 19 hr.

To the mixture was added H20 (500 mL), and the whole mixture was extracted with AcOEt (200 mL X 3). The combined organic layer was washed with H20 (500 mL), dried over Na2SO4, concentrated in vacuo. The residue was purified by column chromatography on silica gel (hexane: ethyl acetate = 10: 1 as eluent) to afford the titled compound as a white solid (25 g, 68%).

IH NMR (CDC13) 8 : 7.37-7. 20 (m, 10H), 3.50 (s, 4H), 2.28 (d, J = 8. 1 Hz, 2H), 2.10 (d, J 8.1 Hz, 2H), 1.81-1. 78 (m, 2H), 1.58-1. 36 (m, 6H), 1.08-1. 00 (m, 2H) ppm.

Preparation 120 Ethyl f cis-4-r (benzvlamino) methyllcvclohexyl} acetate To a cold EtOH (125 mL) was added conc. H2S04 (63 mL) at 0°C, and the mixture was stirred at 0°C for 10 min. To the mixture of {cis-4- [(dibenzylamino) methyl] cyclohexyl} acetonitrile (25 g, 74 mmol) in EtOH (40 mL) was added the mixture of H2SO4 in EtOH solution at 0°C. The reaction mixture was refluxed for 4.5 hr. After evaporation, the residue was cooled at 0°C, H2O (100 mL) was added.

The mixture was basified with NaOH until pH 8. The whole mixture was extracted with AcOEt (lOOmL X 3). The combined organic layer was washed with H20 (200mL), dried over Na2S04, concentrated in vacuo. The residue was purified by column chromatography on silica gel (hexane: ethyl acetate = 40: 1 as eluent) to afford the titled compound as colorless oil (16 g, 61 %).

'H NMR (CDCI3) 6 : 7.37-7. 19 (m, 10H), 4. 16-4. 05 (m, 2H), 3.50 (s, 4H), 2.28-2. 26 (m, 2H), 2.16-2. 07 (m, 2H), 2.00-1. 86 (m, 1H), 1. 83-1. 70 (m, 1H), 1. 58-1. 49 (m, 2H), 1.43-1. 28 (m, 4H), 1.23 (t, J = 8. 1 Hz, 3H), 1.09-0. 99 (m, 2H) ppm.

MS (ESI): 380.26 (M+H) + Preparation 121 2-{ cis-4- (Dibenzylamino) methyllcyclohexvl ethanol To a suspension of LiAIH4 (2.4 g, 63 mmol) in THF (150 mL) was added a solution of ethyl {cis-4-[(benzylamino) methyl] cyclohexyl} acetate (15.8 g, 42 mmol) in THF (200 mL) at 0°C, and the reaction mixture was stirred at 0°C for lhr. To the reaction mixture were added Na2SO4-10H2O (24 g) and KF (2.4 g) and the mixture was stirred at room temperature for 1 hr. The mixture was filtered through a pad of celite and the filtrate was evaporated in vacuo to afford the titled compound as yellow oil (15.9 g).

IH NMR (CDCl3) # : 7.38-7. 19 (m, 10H), 3.61-3. 56 (m, 2H), 3.50 (s, 4H), 2.27 (d, J 7. 7.3 Hz, 2H), 1.85-1. 71 (m, 1H), 1.56-1. 34 (m, 9H), 1.05-0. 96 (m, 2H) ppm. [OH was not observed.] Preparation 122 Dibenzyl {acm-4- (2-phenoxyethyl) cyclohexyl]methyl}amine To a solution of 2-{cs-4 [(dibenzylamino) methyl] cyclohexyl} ethanol (3. 5g, 10 mmol) in toluene (33mL) were added triphenylphosphine (3.0 g, 11 mmol) and phenol (1. 1 g, 11 mmol). To the mixture was added diisopropylazodicarboxylate (2.2 mL, 11 mmol) at 0°C, the mixture was stirred at room temperature for 18 hr. The reaction mixture was concentrated in vacuo. The residue was purified by column chromatography on silica gel (hexane: ethyl acetate = 40: 1 as eluent) to afford the titled compound as colorless oil (4.0 g, 92%).

'H NMR (CDC13) 8 : 7.34-7. 19 (m, 12H), 6.95-6. 84 (m, 3H), 3.91-3. 86 (m, 2H), 3.50 (s, 4H), 2.28 (d, J = 8.1 Hz, 2H), 1.86-1. 73 (m, 1H), 1.62-1. 37 (m, 9H), 1. 11-1. 02 (m, 2H) ppm.

Preparation 123 f [cis-4-(2-Phenoxyethyl)cyclohexyl]methyl}amine hydrochloride To a solution of dibenzylt [cs-4-(2-phenoxyethyl) cyclohexyl] methyl lamine (8.4 g, 23 mmol) in MeOH (80 mL) was added THF (10 mL). To the mixture were added 10% Pd-C (0.8 g) and ammonium formate (3. 3 g, 52 mmol), the reaction mixture was stirred at 62°C for 1 hr. The reaction mixture was filtered through a pad of celite and the filtrate was concentrated in vacuo. To the residue was added H2O (50 mL) and brine (50 mL). The mixture was extracted with CH2CI2 (50 mL x 3), dried over Na2S04, concentrated in vacuo. To this residue was added AcOEt (30 mL), and the mixture was cooled to 0°C. To the mixture was added 4N HCI-AcOEt (5.5 mL). The reaction mixture was stirred at room temperature for 2 hr. The reaction mixture was concentrated in vacuo, filtered and washed with AcOEt. The solid was recrystallized from PrOH (60 mL) to afford the titled compound as a white solid (3.3 g, 52%).

'H NMR (DMSO-d6) 8 : 8.06-7. 78 (m, 3H), 7.31-7. 25 (m, 2H), 6.94-6. 89 (m, 3H), 4.00-3. 96 (m, 2H), 2.75 (d, J = 5.4 Hz, 2H), 1.82-1. 65 (m, 4H), 1.55-1. 31 (m, 8H) ppm.

Preparation 124 Ethyl4-(2-hydroxvethoxv) cvclohexanecarboxylate To a solution of ethyl 1, 4-dioxaspiro [4. 5] decane-8-carboxylate (5.0 g, 23 mmol) in dichloromethane (80 mL) were added triethylsilane (4.1 mL, 26 mmol) and tert- butyldimethylsilyl trifluoromethanesulfonate (0.5 mL, 2.3 mmol) at 0°C. The reaction mixture was stirred at room temperature for 19 hr. To the mixture was added water (100 mL) and the whole mixture was extracted with dichloromethane (50 mL x 3) and the combined organic layer was dried over Na2S04, concentrated in vacuo. The residue was purified by column chromatography on silica gel (hexane: ethyl acetate = 2: 1-1 : 1 as eluent) to afford the titled compound as yellow oil (1.0 g, 20%).

'H NMR (CDCI3, cis-trans mixture) 8 : 4.21-4. 08 (m, 2H), 3.72-3. 50 (m, 4. 5H), 3.32-3. 22 (m, 0. 5H), 2.40-1. 22 (m, 13H) ppm.

Preparation 125 Ethyl 4- (2-phenoxyethoxy) cyclohexanecarboxylate To a solution of ethyl 4- (2-hydroxyethoxy) cyclohexanecarboxylate (1.0 g, 4.7 mmol) in toluene (15 mL) were added triphenylphosphine (1.3 g, 5.1 mmol), phenol (484 mg, 5.1 mmol) and diisopropyl azodicarboxylate (1.0 mL, 5.1 mmol) at 0°C. The mixture was stirred at 0°C for 1 hr, then warmed to room temperature for 2 hr. The reaction mixture was evaporated and the residue was purified by column chromatography on silica gel (hexane : ethyl acetate = 8 : 1 as eluent) to afford the titled compound as yellow oil (761 mg, 56%).

'H NMR (CDCI3, cisltrans mixture) 6 : 7.30-7. 21 (m, 2H), 6.97-6. 82 (m, 3H), 4.16-4. 09 (m, 4H), 3.86-3. 76 (m, 2H), 3.57 (m, 0. 25H), 3.40-3. 30 (m, 0.75H), 2.36-1. 23 (m, 12H) ppm.

Preparation 126 [cis-4- (2-Phenoxyethoxv cyclohexyl l methanol To a suspension of LiAIH4 (148 mg, 3.9 mmol) in THF (4 mL) was added a solution of ethyl 4- (2-phenoxyethoxy) cyclohexanecarboxylate (761 mg, 2.6 mmol) in THF (6 mL) at 0°C, and the mixture was stirred for 30 min. To the reaction mixture were added Na2S04-1OH20 (1.4 g) and KF (0.2 g) and the whole mixture was stirred at room temperature for 0.5 hr. The mixture was filtered through a pad of celite and the filtrate was evaporated in vacuo. The residue was purified by column chromatography on silica gel (hexane: ethyl acetate = 3 : 1 as eluent) to afford the titled compound as colorless oil (111 mg, 17%).

'H NMR (CDC13) 6 : 7.31-7. 23 (m, 2H), 6.97-6. 91 (m, 3H), 4.14-4. 09 (m, 2H), 3.78-3. 74 (m, 2H), 3.64-3. 62 (m, 1H), 3.47 (d, J= 6.1 Hz, 2H), 1.96-1. 88 (m, 2H), 1.59-1. 38 (m, 7H) ppm.

(OH was not observed.) Preparation 127 cis-4- (2-Phenoxyethoxy) cyclohexyllmethvl methanesulfonate To a solution of [cis-4- (2-phenoxyethoxy) cyclohexyl] methanol in dichloromethane (1.5 mL) was added triethylamine (0.1 mL, 0.9 mmol). To the mixture was cooled at 0°C, a solution of methanesulfonyl chloride (60 mg, 0.5 mmol) in dichloromethane (1.5 mL) was added and the mixture was stirred at 0°C for 0.5 hr. To the reaction mixture was added sat. aq. NaHC03 (10 mL) and the mixture was extracted with dichloromethane (15 mL x 3).

The combined organic layers were washed with brine (20 mL x 1), dried over Na2S04, concentrated in vacuo to afford the titled compound as yellow oil (126 mg).

'H NMR (CDC13) 8 : 7.32-7. 25 (m, 2H), 6.97-6. 91 (m, 3H), 4.14-4. 09 (m, 2H), 4.03 (d, J= 7.0 Hz, 2H), 3.77-3. 74 (m, 2H), 3.66-3. 64 (m, 1H), 2. 98 (s, 3H), 1.96-1. 35 (m, 9H) ppm.

Preparation 128 (2-f rcis-4- (Azidomethyl) cyclohexylloxylethoxy) benzene To a solution of [cis-4- (2-phenoxyethoxy) cyclohexyl] methyl methanesulfonate (126 mg, 0.4 mmol) in DMF (3 mL) was added sodium azide (50 mg, 0.8 mmol). The reaction mixture was stirred at 90°C for 3 hr. To the mixture was added water (20 mL), and the mixture was extracted with ethyl acetate (20 mL x 3). The combined organic layers were washed with brine (30 mL), dried over Na2S04, concentrated in vacuo to afford the titled compound as yellow oil (93 mg).

'H NMR (CDCl3) 5 : 7.31-7. 25 (m, 2H), 6.97-6. 91 (m, 3H), 4.14-4. 10 (m, 2H), 3.78-3. 74 (m, 2H), 3.64 (m, 1H), 3.13 (d, J=6. 6Hz, 2H), 1.94-1. 89 (m, 2H), 1.52-1. 35 (m, 7H) ppm.

Preparation 129 f fcis-4- (2-Phenoxvethoxy) cyclohexyllmethy amine To a solution of (2-{[cis-4-(azidomethyl)cyclohexyl]oxy}ethoxy) benzene (93 mg, 0.3 mmol) in methanol (3 mL) was added 10% Pd-C (9 mg) and stirred at room temperature for Ihr under H2 (1 atm). The mixture was filtered through a pad of celite and the filtrate was concentrated in vacuo to afford the titled compound as yellow oil (78 mg).

IH NMR (CDCI3) 8 : 7.31-7. 25 (m, 2H), 6.97-6. 91 (m, 3H), 4.14-4. 10 (m, 2H), 3.78-3. 74 (m, 2H), 3.63-3. 62 (m, 1H), 2.56-2. 54 (m, 2H), 1.93-1. 87 (m, 2H), 1.50-1. 25 (m, 7H) ppm.

[NH2 proton was not observed.] Preparation 130 4-(Methoxymethoyx)-N-{[cis-4-(2-phenoxyethoxy)cyclohexyl]met hyl}benzamide This compound was prepared with { [cis-4- (2-phenoxyethoxy) cyclohexyl] methyl} amine (78 mg, 0.3 mmol) by a procedure similar to that in Preparation 17 as colorless oil (86 mg, 66%) 'H NMR (CDCI3) 6 : 7. 73-7. 70 (m, 2H), 7.30-7. 26 (m, 2H), 7.06-7. 03 (m, 2H), 6.96-6. 91 (m, 3H), 6.16-6. 06 (m, 1H), 5.21 (s, 2H), 4.13-4. 10 (m, 2H), 3.78-3. 74 (m, 2H), 3.66-3. 61 (m, 1H), 3.48 (s, 3H), 3.33-3. 29 (m, 2H), 1. 95-1.93 (m, 2H), 1.67-1. 43 (m, 7H) ppm.

MS (ESI): 414.24 (M+H) + Preparation 131 trans-1- (Aminomethyl)-4- (4-fluorobenzvl) cyclohexanol hydrochloride This compound was prepared with 4-fluorobenzylcyclohexanone (WO 2001028987) by a procedure similar to that in Preparation 16.

'H NMR (DMSO-d6) 6 : 7.80 (br, 3H), 7. 27-7. 01 (m, 4H), 5.00 (br, 1H), 2. 89 (s, 2H), 2.59- 2.42 (m, 2H), 1.78-0. 94 (m, 9H) ppm.

Preparation 132 N, N-Dibenzyl-1-(cis-4-{[(5-nitropyridin-2-yl)oxy]methyl}cycloh exyl)methanamine To a solution of {cìs-4-[(dibenzylamino) methyl] cyclohexyl} methanol (0.64 g, 2.0 mmol) in DMF, NaH (60%, 0.18 g, 4. 4 mmol) was added at 0 °C and the mixture was stirred at room temperature for 1 hour. 2-Chloro-5-nitropyridine (0.96 g, 6.0 mmol) was added at 0 °C, and the mixture was stirred at room temperature overnight. The mixture was quenched with water and diluted with AcOEt. The organic layer was washed with water, dried over MgSO4 and evaporated. The residue was purified by silica gel column chlomatography (hexane-AcOEt 40: 1) to give the titled compound (0.51 g).

'H NMR (CDCI3) 8 : 9.04 (d, J = 2.8 Hz, 1H), 8.33 (dd, J = 2.8, 9.1 Hz, 1H), 7.47-7. 15 (m, 1 OH), 6.75 (d, J=9. 2 Hz, 1H), 4.16 (d, J = 6.9 Hz, 2H), 3.52 (s, 4H), 2.30 (d, J = 7.4 Hz, 2H), 2.02-1. 09 (m, 10H) ppm.

Preparation 133 6-({cis-4-[(Dibenzylamino) methyllcyclohexyl Tmethoxy) peridin-3-amine A mixture of N, N-dibenzyl-1-(cis-4-{ [(5-nitropyridin-2- yl) oxy] methyl} cyclohexyl) methanamine (0.51 g, 1. 1 mmol), Fe (0.31 g, 5.5 mmol), NH4CI (29 mg, 0.55 mmol) in EtOH (10 mL) and water (2 mL) was refluxed for 6 h. To the mixture, water (20 mL) was added and extracted with CH2C12. The extract was washed with water, dried over MgSO¢ and evaporated. The residue was purified by silica gel column chlomatography (hexane-AcOEt 7: 3) to give the titled compound (0.44 g). H NMR (CDC13) 6 : 7.62 (d, J= 3. 0 Hz, 1H), 7.43-7. 16 (m, 10H), 7.01 (dd, J=3. 0,8. 7 Hz, 1H), 6.54 (d, J = 8.7 Hz, 1H), 3.93 (d, J = 7.1 Hz, 2H), 3.51 (s, 4H), 2.29 (d, J = 7.4 Hz, 2H), 2.00-1. 15 (m, 10H) ppm. (NH2 was not observed.) Preparation 134 N, N-DibenzyI-I- (cis-4-f f (5-fluoropyridin-2-yl) oxylmethvl lcyclohexyl) methanamine and N- benzyl-l-phenyl-N- ({cis-4-[(pyridin-2-yloxy)methyl]cyclohexyl}methyl)methanami ne To a solution of 6-({cis-4-[(dibenzylaniino) methyl] cyclohexyl} methoxy) pyridin-3-amine (0.22 g, 0.55 mmol) in EtOH, ethyl nitrite (15% in EtOH, 0.31 mL) was slowly added at 0 °C. To the mixture, HBF4 (42% in water, 0.23 mL) was slowly added at 0 °C and the mixture was stirred at 0 °C for 1 hour. To the mixture, cold ether was added and insoluble purple oil was washed with cold ether. A mixture of the oil and heptane (0.6 mL) was heated at 100 °C for 1 hour. To the mixture, water was added and extracted with CH2CI2.

The extract was dried over MgSO4 and evaporated. The residue was purified by prep. TLC (hexane-AcOEt 10: 1) to give the titled compounds (61 mg and 23 mg).

N,N-Dibenzyl-1-(cis-4-{[(5-fluoropyridin-2-yl)oxy]methyl} cyclohexyl)methanamine (61 mg) 1H NMR (CDC13) 6 : 7.95 (d, J= 2.8 Hz, 1H), 7.43-7. 16 (m, 11H), 6.64 (dd, J= 3.6, 9.1 Hz, 1H), 3. 98 (d, J = 7.1 Hz, 2H), 3.51 (s, 4H), 2.29 (d, J= 7.6 Hz, 2H), 2. 00-1. 13 (m, 10H) ppm.

MS (ESI): 419.25 (M+H) + N-Benzyl-1-phenyl-N-({cis-4-[(pyridin-2-yloxy)methyl]cyclohe xyl}methyl) methanamine (31 mg) 'H NMR (CDCl3) 5 : 8.16-8. 09 (m, 1H), 7.60-7. 50 (m, 1H), 7.43-7. 15 (m, 10H), 6.87-6. 65 (m, 2H), 4.02 (d, J = 6.9 Hz, 2H), 3.51 (s, 4H), 1.92 (d, J = 7.4 Hz, 2H), 2. 02-1. 16 (m, 10H) ppm.

Preparation 135 [@ (cis-4-f f (5-Fluoropvridin-2-vl) oxvlmethvl} cyclohexyl) methvllamine This compound was prepared with N,N-dibenzyl-1-(cis-4-{[(5-fluoropyridin-2- yl) oxy] methyl} cyclohexyl) methanamine by a procedure similar to that in Preparation 123.

IH NMR (CDCI3) 6 : 8. 02-7.94 (m, 1H), 7.40-7. 26 (m, 1H), 6.75-6. 65 (m, 1H), 4.16 (d, J= 7. 3 Hz, 2H), 2.63 (d, J= 6.1 Hz, 2H), 2.12-1. 20 (m, 10H) ppm. (NH2 was not observed.) Preparation 136 (f cis-4-[(Pyridin-2-yloxy)methyl]cyclohexyl}methyl) amine This compound was prepared with N-benzyl-1-phenyl-N-({cis-4-[(pyridin-2- yloxy) methyl] cyclohexyl} methyl) methanamine by a procedure similar to that in Preparation 123.

'H NMR (CDC13) 6 : 8.20-8. 11 (m, 1H), 7.62-7. 51 (m, 1H), 6.91-6. 70 (m, 2H), 4.20 (d, J= 7.3 Hz, 2H), 2.62 (d, J= 6.1 Hz, 2H), 2.12-1. 20 (m, 10H) ppm. (NH2 was not observed.) Preparation 137 N, N-Dibenzyl-1,4-dioxaspiro[4.5]decane-8-carboxamide This compound was prepared with 1, 4-dioxaspiro [4.5] decane-8-carboxylic acid and dibenzylamine by a procedure similar to that in Preparation 1.

IH NMR (CDCI3) 8 : 7.40-7. 23 (m, 10H), 4.59 (s, 2H), 4.46 (s, 2H), 3.95-3. 93 (m, 4H), 2. 61-2. 51 (m, 1H), 2.10-1. 95 (m, 2H), 1. 85-1. 77 (m, 4H), 1.53-1. 42 (m, 2H) ppm.

Preparation 138 N, N-Dibenz-1- (1, 4-dioxaspirof4. 51dec-8-yl) methanamine This compound was prepared with N,N-dibenzyl-1, 4-dioxaspiro [4.5] decane-8- carboxamide by a procedure similar to that in Preparation 2.

IH NMR (CDCI3) 8 : 7.39-7. 18 (m, 10H), 3.96-3. 86 (m, 4H), 3.79-3. 71 (m, 1H), 3.51 (s, 4H), 2.24 (d, J= 7.2 Hz, 2H), 1.92-1. 80 (m, 3H), 1.75-1. 40 (m, 4H), 1.14-0. 98 (m, 2H) ppm.

Preparation 139 2-({4-[(Dibenzylamino)methyl]cyclohexyl}oxy)ethanol To a solution of N,N-dibenzyl-1-(1, 4-dioxaspiro [4.5] dec-8-yl) methanamine (2.8 g, 7.9 mmol) in dichloromethane (70 mL) was added diisobutylaluminium hydride (0.93 M in hexane, 15 mL, 16 mmol) at 0 °C under nitrogen and the mixture was stirred for 3 hours.

Na2S04-lOH2O (12 g) and KF (2 g) were added to the mixture and the resulting mixture was stirred for 2 hours at room temperature. After filtration, the filtrate was evaporated to affors the titled compound. (2.6 g) 1H NMR (CDC13) 6 : 7.42-7. 16 (m, 10H), 3.75-3. 50 (m, 2H), 3.59-3. 06 (m, 7H), 2. 35-2. 10 (m, 2H), 2. 11-0. 63 (m, 9H) ppm. (OH was not observed.) Preparation 140 N,N-Dibenzyl-1-{4-[2-(4-fluorophenoxy)ethoxy]cyclohexyl}meth anamine This compound was prepared with 2-({4- [(dibenzylamino) methyl3cyclohexyl} oxy) ethanol by a procedure similar to that in Preparation 33.

1H NMR (CDCI3) 8 : 7.39-7. 17 (m, 10H), 7.02-6. 77 (m, 4H), 4.17-4. 00 (m, 2H), 3.85-3. 60 (m, 2H), 3.58-3. 49 (m, 5H), 2.26-2. 16 (m, 2H), 1.81-1. 11 (m, 9H) ppm.

Preparation 141 (f 4-[2-(4-Fluorophenoxy)ethoxy]cyclohexyl}methyl) amine This compound was prepared with N,N-dibenzyl-1-{4-[2-(4- fluorophenoxy) ethoxy] cyclohexyl} methanamine by a procedure similar to that in Preparation 123.

'H NMR (CDCl3) 8 : 7.03-6. 82 (m, 4H), 4.12-4. 02 (m, 2H), 3.84-3. 70 (m, 2H), 3.66-3. 21 (m, 1H), 2.62-2. 51 (m, 2H), 2. 17-0. 84 (m, 9H) ppm. (NH2 was not observed.) Preparation 142 N,N-Dibenzyl-1-{cis-4-[2-(2-fluorophenoxy)ethyl]cyclohexyl}m ethanamine This compound was prepared with 2-{cis-4-[(dibenzylamino)methyl]cyclohexyl}ethanol and 2-fluorophenol by a procedure similar to that in Preparation 33.

'H NMR (CDCI3) 8 : 7.45-6. 81 (m, 14H), 4.00-3. 91 (m, 2H), 3.51 (s, 4H), 2.28 (d, Y= 7. 6 Hz, 2H), 1.93-0. 94 (m, 12H) ppm.

Preparation 143 ( cis-4-f2- (2-Fluorophenoxv) ethllcyclohexyl methyl) amine hydrochloride This compound was prepared with N,N-dibenzyl-1-{cis-4-[2-(2- fluorophenoxy) ethyl] cyclohexyl} methanamine by a procedure similar to that in Preparation 123.

1H NMR (DMSO-d6) 8 : 8.04 (br, 3H), 7.26-7. 06 (m, 3H), 7.00-6. 87 (m, 1H), 6.06 (t, J= 6.1 Hz, 2H), 2.74 (d, J= 7.3 Hz, 2H), 1.88-1. 29 (m, 12H) ppm.

Preparation 144 Benzyl (f cis-4-[(2-fluorophenoxy)methyl]cyclohexyl}methyl)carbamate This compound was prepared with benzyl { [cis-4- (hydroxymethyl) cyclohexyl] methyl} carbamate and 2-fluorophenol by a procedure similar to that in Preparation 33. lH NMR (CDCl3) 6 : 7.44-7. 22 (m, 5H), 7.14-6. 81 (m, 4H), 5.10 (s, 2H), 4.85-4. 68 (m, 1H), 3.96-3. 82 (m, 2H), 3.24-3. 11 (m, 2H), 2.14-1. 19 (m, 10H) ppm.

Preparation 145 (t cis-4-f (2-Fluorophenoxy) methyllcyclohexyl Tmethyl) amine This compound was prepared with benzyl ({cis-4-[(2- fluorophenoxy) methyl] cyclohexyl} methyl) carbamate by a procedure similar to that in Preparation 79.

MS (ESI) : 237.10 (M+H) + Preparation 146 Benzyl (f cis-4-f (3-fluorophenoxy) methyllcvclohexyl) methvl) carbamate This compound was prepared with benzyl { [cis-4- (hydroxymethyl) cyclohexyl] methyl} carbamate and 3-fluorophenol by a procedure similar to that in Preparation 33. 'H NMR (CDCI3) 8 : 7. 37-7. 30 (m, SH), 7.26-7. 19 (m, 1 H), 6.68-6. 58 (m, 3H), 5.10 (s, 2H), 4.80-4. 72 (m, 1H), 3.84-3. 81 (m, 2H), 3.20-3. 15 (m, 2H), 2.13-1. 85 (m, 1H), 1. 84-1. 30 (m, 9H) ppm.

MS (ESI) : 372.01 (M+H) + Preparation 147 ( cis-4-f (3-Fluorophenoxy) methyllcyclohexyl Smethyl) amine This compound was prepared with benzyl ( {cis-4- [ (3- fluorophenoxy) methyl] cyclohexyl} methyl) carbamate by a procedure similar to that in Preparation 79.

'H NMR (CDCI3) 6 : 7.25-7. 13 (m, 1H), 6.88-6. 41 (m, 3H), 3. 85-3. 81 (m, 2H), 2.48-2. 85 (m, 2H), 2.29-1. 87 (m, 1H), 1.84-1. 02 (m, 9H) ppm. (NH2 was not observed.) MS (ESI) : 238. 15 (M+H) + Preparation 148 Diethyl icis-4-f (dibenzvlamino) methyllcyclohexyl} methyl) malonate To a stirred mixture of diethyl malonate (1. 1 g, 7.1 mmol) in DMF (15 mL) was added sodium hydride (0. 28 g, 7.1 mmol) at 0 °C. After stirring for 15 minutes, {cis-4- [(dibenzylamino) methyl] cyclohexyl} methyl methanesulfonate (2.7 g, 6.7 mmol) in DMF (5 mL) was added, and the mixture was heated at 120 °C for 1 day. The mixture was cooled, quenched with sat. aq. NaHC03, and extracted with AcOEt. The organic layer was washed with H2O and brine, dried over MgS04, and concentrated. The residue was purified by silica gel column chromatography (hexane: AcOEt = 7: 1) to give the titled compound (0.38 g). 'H NMR (CDC13) 8 : 7.42-7. 17 (m, 10H), 4.17 (q, J = 7.2 Hz, 4H) 3.50 (s, 4H), 3.31 (t, J = 7.7 Hz, 1H), 2.27 (d, J = 7.5 Hz, 2H), 1.95-1. 27 (m, 10H), 1.25 (t, J = 7.2 Hz, 6H), 1.10- 0.90 (m, 2H) ppm.

MS (ESI) : 466.14 (M+H) + Preparation 149 3-{cis-4-[(Dibenzylamino)methyl]cyclohexyl}propanoic acid A mixture of diethyl ({cis-4-[(dibenzylamino) methyl] cyclohexyl} methyl) malonate (0.38 g, 0.82 mmol) and 2 N aq. HCl (4 mL) in acetic acid (4 mL) was stirred at 120 °C for 3 days.

The mixture was concentrated, and dried in vacuum to give the titled compound (0. 38 g).

MS (ESI) : 366.14 (M+H) +, 364.15 (M-H)- Preparation 150 3-{ cis-4-f (Dibenzvlamino) methyllcyclohexyl Wpropan-1-ol This compound was prepared with 3-{cis-4- [ (dibenzylamino) methyl] cyclohexyl} propanoic acid by a procedure similar to that in Preparation 103.

'H NMR (CDC13) 6 : 7.43-7. 17 (m, 10H), 3.67-3. 50 (m, 2H), 3.50 (s, 4H), 2.27 (d, J = 7.5 Hz, 2H), 1.95-0. 70 (m, 14H) ppm. (OH was not observed.) MS (ESI) : 352.14 (M+H) + Preparation 151 N, N-Dibenzyl-l-fcis-4- (3-phenoxypropyl) cvclohexyllmethanamine This compound was prepared with 3-{cis-4-[(dibenzylamino) methyl] cyclohexyl} propan- 1-ol by a procedure similar to that in Preparation 33.

'H NMR (CDC13) 8 : 7.43-7. 16 (m, 12H), 6.97-6. 80 (m, 3H), 3. 88 (t, J= 6.8 Hz, 2H), 3.51 (s, 4H), 2.34-2. 25 (m, 2H), 1.96-0. 70 (m, 14H) ppm.

MS (ESI) : 428.19 (M+H) + Preparation 152 f [cis-4- (3-Phenoxvpropvl) cyclohexyllmethyl amine This compound was prepared with N,N-dibenzyl-1-[cis-4-(3- phenoxypropyl) cyclohexyl] methanamine by a procedure similar to that in Preparation 103.

. MS (ESI) : 248. 17 (M+H) + Preparation 153 Dibenzvl ({cis-4-[(4-fluorophenoxy)methyl]cyclohexyl}methyl) amine This compound was prepared with {cis-4-[(dibenzylamino) methyl] cyclohexyl} methanol (1.5 g, 4.6 mmol) and 4-fluorophenol (570 mg, 5.1 mmol) by a procedure similar to that in Preparation 104 as a white solid (1.9 g, quant.).

'H NMR (CDCl3) 5 : 7.38-7. 21 (m, 10H), 6.97-6. 91 (m, 2H), 6.79-6. 74 (m, 2H), 3.62 (d, J = 6. 8 Hz, 2H), 3.51 (s, 4H), 2.30 (d, J = 7.3 Hz, 2H), 1. 85-1. 23 (m, 10H) ppm.

Preparation 154 (f cis-4-r (4-Fluorophenoxy) methyllcvclohexvl methyl) amine hydrochloride This compound was prepared with dibenzyl ( {cis-4- [ (4- fluorophenoxy) methy] cyclohexyl} methyl) amine (1.9 g, 4.7 mmol) by a procedure similar to that in Preparation 105 as a white solid (543 mg, 43%).

'H NMR (DMSO-d6) 8 : 7.96-7. 79 (m, 2H), 7.14-7. 08 (m, 2H), 6.97-6. 92 (m, 2H), 3.86 (d, J = 5.4 Hz, 2H), 2.76 (d, J = 5.4 Hz, 2H), 1. 91-1. 79 (m, 2H), 1.51-1. 46 (m, 8H) ppm.

Preparation 155 Benzyl (f cis-4-[(3-methoxyphenoxy)methyl]cyclohexyl}methyl) carbamate This compound was prepared with benzyl { [cis-4- (hydroxymethyl) cyclohexyl] methyl} carbamate and 3-methoxyphenol by a procedure similar to that in Preparation 33.

1H NMR (CDC13) 8 : 7.41-7. 29 (m, SH), 7.17 (t, J= 8.1 Hz, 1H), 6.53-6. 43 (m, 3H), 5.16 (s, 2H), 4. 80-4. 67 (1H, m), 3.81 (d, J = 6.9 Hz, 2H), 3.79 (s, 3H), 3.17 (d, J= 6.6 Hz, 2H), 2.21-1. 90 (m, 1H), 1. 78-1. 30 (m, 9H) ppm.

MS (ESI) : 384.17 (M+H) + Preparation 156 ({cis-4-[(3-Methoxyphenoxy)methyl]cyclohexyl}methyl)amine This compound was prepared with benzyl ( {cis-4- [ (3- methoxyphenoxy) methyl] cyclohexyl} methyl) carbamate by a procedure similar to that in Preparation 79. 'H NMR (DMSO-d6) 8 : 7.16 (t, J= 8.3 Hz, IH), 6. 56-6. 42 (m, 3H), 3.84 (d, J= 6.9 Hz, 2H), 3.72 (s, 3H), 2.47-2. 45 (m, 2H), 1.97-1. 82 (m, 1H), 1.61-1. 27 (m, 9H) ppm. NH2 was not observed.

MS (ESI) : 250.13 (M+H) + Preparation 157 Diethyl but-3-en-1-ylf2- (4-fluorophenoxv) ethvllmalonate To a solution of diethyl but-3-en-1-ylmalonate (2.00 g, 9.3 mmol) in DMF was added NaH (448.0 mg, 11.2 mmol) at 0 °C. The mixture was stirred at 0 °C for 30 min. Then 1- (2- bromoethoxy) -4-fluorobenzene was added and the mixture was stirred at room temperature for 10.5 hr. The reaction mixture was quenched with water and the mixture was extracted with AcOEt. The organic layer was dried over Na2S04, filtered and evaporated. The crude product was purified by silica gel column chromatography (hexane: AcOEt = 200: 1 to 20: 1) to give the titled compound (2.2 g).

'H NMR (CDC13) 8 : 7.00-6. 90 (m, 2H), 6.80-6. 76 (m, 2H), 5. 85-5. 67 (m, 1H), 5.07-4. 96 (m, 2H), 4.30-4. 20 (m, 4H), 4.05-3. 95 (m, 2H), 2.45-2. 40 (m, 2H), 2.15-1. 92 (m, 4H), 1.29-1. 15 (m, 6H) ppm.

Preparation 158 Ethyl 2-f 2- 4-fluorophenoxv) ethyllhex-5-enoate To a solution of diethyl but-3-en-1-yl [2- (4-fluorophenoxy) ethyl] malonate (2. 21 g, 6.27 mmol) in DMSO (20 mL) and H20 (0.11 mL), LiCI (798 mg, 18. 8 mmol) was added and the mixture was stirred at 150 °C for 2 days. Then the mixture was cooled to room temperature and was poured into AcOEt/H2O. The mixture was extracted twice with AcOEt and the combined organic layers were washed with brine. The organic layer was dried over Na2S04, was filtered and evaporated. The crude product was purified by silica gel column chromatography (hexane: AcOEt = 100: 1-20: 1) to give the titled compound (1.24 g, 4.22 mmol) IH NMR (CDC13) 8 : 7.00-6. 90 (m, 2H), 6. 83-6. 75 (m, 2H), 5. 86-5. 70 (m, 1H), 5.10-4. 95 (m, 2H), 4.20-4. 10 (m, 2H), 4.04-3. 85 (m, 2H), 2.72-2. 55 (m, 1H), 2.20-1. 52 (m, 6H), 1.24 (t, J = 6.8 Hz, 3H) ppm.

Preparation 159 2-f2- (4-Fluorophenoxv) ethyllhex-5-en-1-ol To a suspension of LAH (167.7 mg, 4.42 mmol) in THF (30 mL) the solution of ethyl 2- [2- (4-fluorophenoxy) ethyl] hex-5-enoate (1.24g, 4.42 mmol) in THF (15 mL) was added at 0 °C. Then the mixture was stirred at room temperature for 5.5 hr. The reaction was quenched by Na2SO4 lOH20 (2.0 g, 6.21 mmol) and KF (0.25 g, 43.0 mmol). The mixture was stirred at room temperature overnight. The mixture was filtered through a pad of celite and the filtrate was evaporated to give the titled compound (1.03 g).

'H NMR (CDC13) 6 : 7.00-6. 94 (m, 2H), 6. 86-6. 81 (m, 2H), 5.89-5. 70 (m, 1H), 5.10-4. 90 (m, 2H), 4.10-3. 95 (m, 2H), 3.70-3. 55 (m, 2H), 2.20-2. 05 (m, 2H), 1.90-1. 72 (m, 4H), 1.60-1. 40 (m, 1H) ppm. (- OH was not observed.) Preparation 160 4-(4-Fluorophenoxy)-2-(2-oxiran-2-vlethyl) butan-1-ol To a solution of 2- [2- (4-fluorophenoxy) ethyl] hex-5-en-1-ol (1.03 g, 4.32 mmol) in CH2CI2 (40 mL), NaHC03 (942.9 mg, 11.2 mmol) and mCPBA (1.40 g, 8.11 mmol) was added at 0 °C and the mixture was stirred at 0 °C for 30 min. Then the mixture was allowed to warm to room temperature and was stirred overnight. The reaction was quenched by sat.

Na2S203aq and the mixture was stirred for 1 hr. The mixture was extracted 3 times with CH2C12 and the combined organic layers were washed with sat. NaHC03 and brine. The organic layer was dried over Na2S04, filtered and evaporated to give the titled compound (All, 43. 6 mmol).

'H NMR (CDCl3) # : 7. 01-6. 94 (m, 2H), 6.90-6. 80 (m, 2H), 4. 15-3. 96 (m, 2H), 3.70-3. 58 (m, 2H), 2.96-2. 90 (m, 1H), 2.80-2. 74 (m, 1H), 2.53-2. 47 (m, 1H), 1.90-1. 78 (m, 4H), 1.70-1. 45 (m, 3H) ppm. (OH was not observed.) Preparation 161 {5-[2-(4-Fluorophenoxy)ethyl]tetrahydro-2H-pyran-2-yl}methan ol To a solution of 4-(4-fluorophenoxy)-2-(2-oxiran-2-ylethyl)butan-1-ol (1.11 g, 4.37 mmol) in CH2CI2 (130 mL), p-TsOH-H20 (12.4 mg, 0. 066mmol) was added. The mixture was stirred at room temperature for 4 hr. The reaction was quenched by sat. NaHC03aq, and the mixture was extracted with CH2Cl2. The organic layer was dried over Na2S04, filtered and evaporated. The crude product was purified by silica gel column chromatography (hexane-AcOEt 9: 1-3: 2) to give the titled compound (898. 6 mg).

'H NMR (CDC13) $ : 7.05-6. 90 (m, 2H), 6.86-6. 77 (m, 2H), 4.10-3. 80 (m, 3H), 3.70-3. 10 (m, 4H), 2.10-1. 70 (m, 3H), 1.60-1. 20 (m, 4H) ppm. (-OH was not observed.) Preparation 162 2-(AzidomethYl)-5-F2-(4-fluorophenoxy) ethylltetrahydro-2H-pyran This compound was prepared with benzyl {5-[2-(4-fluorophenoxy)ethyl]tetrahydro-2H- pyran-2-yl} methanol by a procedure similar to that in Preparation 67.

IH NMR (CDCI3) 5 : 7.00-6. 93 (m, 2H), 6.85-6. 79 (m, 2H), 4.10-3. 85 (m, 3H), 3.70-2. 89 (m, 4H), 2.10-1. 70 (m, 3H), 1.60-1. 10 (m, 4H) ppm.

Preparation 163 ( 5-[2-(4-Fluorophenoxytethylltetrahydro-2H-pyran-2-Yl imethyl) amine This compound was prepared with 2- (azidomethyl)-5- [2- (4- fluorophenoxy) ethyl] tetrahydro-2H-pyran by a procedure similar to that in Preparation 8.

IH NMR (CDCl3) 8 : 7.05-6. 90 (m, 2H), 6.86-6. 75 (m, 2H), 4.06-3. 80 (m, 3H), 3.49 (s, 2H), 2.75-2. 68 (m, 2H), 2. 15-1. 10 (m, 7H) ppm MS (ESI): 254.17 (M+H)+ Preparation 164 Ethyl 4- (4-methoxybenzylidene) cyclohexanecarboxylate A mixture of NaH (60%, 1.0 g, 25 mmol) and DMSO (20 mL) was stirred for 2 hours at 80°C under nitrogen. After cooling to room temperature, Diethyl 4- methoxybenzylphosphate (5.2 g, 20 mmol) was added to the mixture. After 1 hour, to the mixture was added ethyl 4-oxocyclohexanecarboxylate (3.4 g, 20 mmol) and the reaction mixture was stirred for 3 hours at 60°C. The mixture was quenched with water and the whole was extracted with ethyl acetate. The organic layer was washed with water and brine, dried and evaporated. The residue was purified by silica gel chromatography (hexane: ethyl acetate= 10: 1) to afford the titled compound (0.39 g) 1H NMR (CDCl3) 6 : 7.12 (d, J = 8. 7 Hz, 2H), 6. 86 (d, J = 8.7 Hz, 2H), 6.22 (s, 1H), 4.13 (q, J= 7.1 Hz, 2H), 3.81 (s, 3H), 2.90-2. 78 (m, 1H), 2.57-1. 47 (m, 8H), 1.26 (t, J= 7.1 Hz, 3H) ppm.

Preparation 165 Ethyl cis-4-(4-methoxybenzyl) cyclohexanecarboxylate A mixture of ethyl 4- (4-methoxybenzylidene) cyclohexanecarboxylate (0.39 g, 1.4 mmol) and 10% Pd on C (40 mg) in methanol (20 mL) was stirred for 4 hours under hydrogen (4 kg/cm2). After filtration through a pad of celite, the filtrate was evaporated. The residue was purified by silica gel chromatography (hexane: ethyl acetate = 12: 1) to afford the titled compound (0.29 g) lHNMR (CDCI3) 6 : 7.06 (d, J = 8. 7 Hz, 2H), 6.82 (d, J=8. 7Hz, 2H), 4.15 (q, J=7.1 Hz, 2H), 3.79 (s, 3H), 2.57-2. 43 (m, 3H), 2.10-1. 92 (m, 2H), 1. 69-1.17 (m, 10H) ppm.

Preparation 166 [cis-4-(4-MethoxYbenzyl) cyclohexyllmethanol This compound was prepared with ethyl_cis-4-(4- methoxybenzyl) cyclohexanecarboxylate by a procedure similar to that in Preparation 121.

'H NMR (CDC13) 6 : 7.06 (d, J=8.7 Hz, 2H), 6.82 (d, J=8.7 Hz, 2H), 3.79 (s, 3H), 3.61- 3.54 (m, 2H), 2.52 (d, J = 7.6 Hz, 2H), 1.81-1. 20 (m, 9H) ppm. (-OH was not observed.) Preparation 167 1-f fcis-4- (Azidomethvl) cyclohexyllmethyl}-4-methoxvbenzene This compound was prepared with [cis-4-(4-methoxybenzyl)cyclohexyl]methanol by a procedure similar to that in Preparation 67.

'H NMR (CDC13) 6 : 7.06 (d, J = 8.6 Hz, 2H), 6. 83 (d, J = 8.6 Hz, 2H), 3.79 (s, 3H), 3.25 (d, J = 7.3 Hz, 2H), 2.52 (d, J = 7.6 Hz, 2H), 1.83-1. 67 (m, 2H), 1.62-1. 22 (m, 7H) ppm.

Preparation 168 {[cis-4-(4-Methoxybenzyl)cyclohexyl]methyl}amine This compound was prepared with 1-{ [cis-4-(azidomethyl) cyclohexyl] methyl}-4- methoxybenzene by a procedure similar to that in Preparation 8.

MS (ESI): 234.15 (M+H)+ Preparation 169 Methyl 3-(2-phenylethoxy) cyclohexanecarboxvlate To a stirred mixture of methyl 3-oxocyclohexanecarboxylate (0.52 g, 3.3 mmol) (J. Am.

Chem. Soc. 1987, 109, 3493-3494. ) and phenethyl alcohol (0.48 mL, 4.0 mmol) in CH2C12 (5 mL) were added bismuth (ni) chloride (0.53 g, 1.7 mmol) and triethylsilane (1.2 mL, 7.3 mmol) at room temparature. After stirring for 1 day at room temparature, the mixture was filtered over celite, and the filterate was concentrated. The residue was purified by silica gel colomn chromatography (hexane-AcOEt 10: 1) to give the titled compound (0.77 g) as cis- trans (1: 1) mixture.

'H NMR (CDCI3) 8 : 7.35-7. 15 (m, 5H), 3.77-3. 60 (m, 2H), 3.67 (s, 3H), 3.35-3. 15 (m, 0. 5H), 2.87 (t, J= 7.4 Hz, 2H), 2.73-2. 58 (m, 0.5H), 2. 37-1. 10 (m, 9H) ppm.

Preparation 170 F3- (2-Phenylethoxv) cyclohexyll methanol This compound was prepared as cis-trans (1: 1) mixture with methyl 3- (2- phenylethoxy) cyclohexanecarboxylate by a procedure similar to that in preparation 121.

'H NMR (CDCl3) # : 7.35-7. 16 (m, 5H), 3.74-3. 17 (m, 5H), 2. 87 (t, J = 7.3 Hz, 2H), 2.15- 0.80 (m, 9H) ppm. (-OH was not observed.) Preparation 171 cis-3-(Azidomethyl)cyclohexyl 2-phenylethyl ether This compound was prepared with [3-(2-phenylethoxy) cyclohexyl] methanol by a procedure similar to that in preparation 67.

'H NMR (CD3) 8 : 7.35-7. 16 (m, 5H), 3. 68 (t, J = 7.3 Hz, 2H), 3.30-3. 15 (m, 1H), 3.16 (d, J = 6.6 Hz, 2H), 2.87 (t, J = 7.3 Hz, 2H), 2.13-1. 96 (m, 2H), 1. 88-1. 50 (m, 3H), 1.33-0. 80 (m, 4H) ppm.

Preparation 172 {[(cis-3-(2-Phenylethoxy)cyclohexyl)methyl]amine This compound was prepared with cis-3- (azidomethyl) cyclohexyl 2-phenylethyl ether by a procedure similar to that in preparation 8.

MS (ESI) : 234.25 (M+H) + Preparation 173 Ethyl 4- (benzvlidene) cyclohexanecarboxylate This compound was prepared with diethyl 4-methoxybenzylphosphate by a procedure similar to that in Preparation 163.

'H NMR (CDCI3) 8 : 7. 37-7. 12 (m, 5H), 6.29 (s, 1H), 4.13 (q, J = 7.1 Hz, 2H), 2.92-2. 79 (m, 1H), 2.59-2. 36 (m, 2H), 2. 32-2. 16 (m, 1H), 2.14-1. 91 (m, 2H), 1.77-1. 46 (m, 3H), 1.26 (t, J = 7. 1 Hz, 3H) ppm.

Preparation 174 Ethyl cis-4-benzylcyclohexanecarboxylate This compound was prepared with ethyl 4- (benzylidene) cyclohexanecarboxylate by a procedure similar to that in Preparation 164.

H NMR (CDC13) 8 : 7. 34-7. 06 (m, 5H), 4.15 (q, J = 7.1 Hz, 2H), 2.60-2. 42 (m, 3H), 2.10- 1. 88 (m, 2H), 1.75-1. 13 (m, 10H) ppm.

Preparation 175 (cis-4-Benzyvclohexvl) methanol This compound was prepared with ethyl cis-4-benzylcyclohexanecarboxylate by a procedure similar to that in Preparation 121.

H NMR (CDCI3) b : 7.36-7. 07 (m, 5H), 3.79-3. 49 (m, 3H), 2.64-2. 49 (m, 2H), 1.93-1. 18 (m, 10H) ppm. (-OH was not observed.) Preparation 176 l-Trcis-4-(Azidomethyl) cYclohexyllmethyl Tbenzene This compound was prepared with (cis-4-benzylcyclohexyl) methanol by a procedure similar to that in Preparation 67.

'H NMR (CDCl3) # : 7.35-7. 19 (m, 5H), 3.26 (d, J = 7.3 Hz, 2H), 2.58 (d, J = 7.6 Hz, 2H), 1.88-1. 69 (m, 2H), 1.63-1. 19 (m, 7H) ppm.

Preparation 177 {[cis-4-(4-Benzyl0cyclohexyl]methyl}amine This compound was prepared with 1-{ [cis-4-(azidomethyl) cyclohexyl] methyl} benzene by a procedure similar to that in Preparation 8.

MS (ESI): 244.15 (M+H) + Preparation 178 (4R-3-Hex-5-enoyl-4-isopropyl-1, 3-oxazolidin-2-one To a solution of hex-5-enoic acid (11.24 g, 87.0 mmol), (4R)-4-isopropyl-1, 3-oxazolidin- 2-one (12.91 g, 113. 1 mmol) and DMAP (1. 06 g, 8.70 mmol) in CH2CI2, was added DCC (23.33 g, 113. 1 mmol) at 0 °C and the mixture was stirred at 0 °C for 15 min. Then the mixture was stirred at room temperature overnight and was filtered through a pad of celite and the filtrate was washed with sat. NaHCO3aq. The organic layer was dried over Na2SO4, filtered and evaporated. The crude product was purified by silica gel column chromatography (hexane-AcOEt 20: 1-10: 1) to give the titled compound (16.60 g, 73.7 mmol).

1H NMR (CDCI3) 6 : 5.90-5. 70 (m, 1H), 5.10-4. 95 (m, 2H), 4.50-4. 42 (m, 1H), 4.35-4. 15 (m, 2H), 3.10-2. 80 (m, 2H), 2.46-2. 30 (m, 1H), 2.20-2. 08 (m, 2H), 1.90-1. 68 (m, 2H), 0.92 (d, J = 7.1 Hz, 3H), 0.88 (d, J= 6.9 Hz, 3H) ppm Preparation 179 (4R)-3-f (2S)-2-f (Benzyloxy) methyllhex-5-enoyl}-4-isopropyl-1, 3-oxazolidin-2-one To a solution of (4R)-3-hex-5-enoyl-4-isopropyl-1, 3-oxazolidin-2-one (16.60 g, 73.7 mmol) in CH2Cl2, was added TiCI4 (8.89 mL, 81. 1 mmol) at 0 °C and the mixture was stirred at 0 °C for 5 min. To the resulting slurry diisopropylethylamine (14.1 mL, 81. 1 mmol) was added and the mixture was stirred at 0 °C for 1 hr. Benzyl chloromethyl ether (23.1 mL, 165.8 mmol) was added dropwise and the mixrure was allowed to warm to room temperature. The mixture was stirred at room temperature for 1. 5 hr and quenched by careful addition of sat. HCI aq. The mixture was extracted twice with CH2Cl2 and the combined organic layers were dried over Na2S04, filtered and evaporated. The crude product was purified by silica gel column chromatography (hexane: AcOEt = 20: 1 tol 1 : 2) to give the titled compound (18. 74 g, 54.3 mmol).

'H NMR (CDC13) 6 : 7. 38-7. 21 (m, 5H), 5.86-5. 68 (m, 1H), 5.05-4. 90 (m, 2H), 4.57-4. 40 (m, 3H), 4.35-4. 11 (m, 3H), 3.77-3. 60 (m, 2H), 2.42-2. 25 (m, 1H), 2.15-2. 00 (m, 2H), 1.94- 1.78 (m, 1H), 1.65-1. 50 (m, 1H), 0.88 (d, J = 7.1 Hz, 3H), 0. 78 (d, J = 6.9 Hz, 3H) ppm.

Preparation 180 (2R)-2-f (Benzyloxy) methyllhex-5-en-1-ol To a suspension of LAH (6.18 g, 162.9 mmol) in THF (250 mL) the solution of (4R)-3- {(2S)-2-[(benzyloxy) methyl] hex-5-enoyl}-4-isopropyl-1, 3-oxazolidin-2-one (18.74 g, 54.3 mmol) in THF (50 mL) was added at 0 °C. Then the mixture was stirred at 0 °C for 30 min.

The reaction was quenched by Na2S04-I OH20 (26.24 g, 81.3 mmol) and KF (3.30 g, 56.7 mmol). The mixture was stirred at room temperature for 1 hr. The mixture was filtered through a pad of celite and the filtrate was evaporated to give the crude product. Then the crude product was purified by silica gel column chromatography (hexane: AcOEt = 4: 1) to give the titled compound (10. 51g, 47.7 mmol).

'H NMR (CDCI3) S : 7.40-7. 23 (m, 5H), 5.90-5. 68 (m, 1H), 5.06-4. 92 (m, 2H), 4.57-4. 48 (m, 2H), 3.81-3. 58 (m, 3H), 3.51-3. 45 (m, 1H), 2.59-2. 49 (m, 1H), 2.13-2. 03 (m, 2H), 1.98-1. 82 (m, 1H), 1.50-1. 29 (m, 2H) ppm.

Preparation 181 f (5S)-5-[ (Benzyloxy) methylltetrahydro-2H-pyran-2- methanol To a solution of (2R)-2-[(benzyloxy) methylthex-5-en-1-ol (10.51g, 47.7 mmol) in CH2C12 (300 mL) were NaHC03 (16. 03 g, 190.8 mmol) and mCPBA (23. 85 g, 138. 2 mmol) at 0 °C. Then the mixture was stirred at room temperature for 2 days. The reaction was quenched with sat. Na2S203 aq at 0 °C and the mixture was stirred at room temperature for 1.5 hr. The mixture was separated and the aqueous layer was extracted twice with CH2Cl2.

The combined organic layers were washed with sat. NaHC03aq and brine. The organic layer was dried over MgS04and filtered.

To the obtained solution, was added p-TsOH (907.3 mg, 4.77 mmol). The mixture was stirred at 55 °C for 1.75 hr. The mixture was cooled to room temperature. The reaction mixture was quenched by sat. NaHCO3aq, and the mixture was extracted with CH2CI2. The combined organic layers were dried over Na2SO4, and the crude product was purified by silica gel column chromatography (hexane: AcOEt = 3: 1 to 3: 2) to give the titled compound (5.61 g, 23.7 mmol).

'H NMR (CDC13) 6 : 7.45-7. 15 (m, 5H), 4.60-4. 42 (m, 2H), 4. 20-3. 97 (m, 1H), 3.73-3. 10 (m, 6H), 2. 10-1. 20 (m, 5H) ppm. (OH was not observed.) Preparation 182 (5S)-2- (Azidomethvl)-5-f (benzyloxy) methylltetrahydro-2H-pyran This compound was prepared with {(5S)-5-[(benzyloxy) methyl] tetrahydro-2H-pyran-2- yl} methanol by a procedure similar to that in Preparation 67.

'H NMR (CDCl3) 6 : 7.40-7. 20 (m, 5H), 4.62-4. 42 (m, 2H), 4. 18-3. 97 (m, 1H), 3.73-3. 10 (m, 6H), 2.00-1. 82 (m, 2H), 1.75-1. 20 (m, 3H) ppm.

Preparation 183 (t (5S)-5-r (Benzyloxy) methylltetrahydro-2H-pyran-2-yl Smethyl) amine This compound was prepared with (5S)-2- (azidomethyl)-5- [ (benzyloxy) methyl] tetrahydro-2H-pyran by a procedure similar to that in Preparation 8.

'H NMR (CD13) 6 : 7.36-7. 26 (m, 5H), 4.60-4. 47 (m, 2H), 4.18-3. 97 (m, 1H), 3.30-3. 48 (m, 2H), 3.30-3. 14 (m, 2H), 2.71-2. 66 (m, 3H), 2. 00-1.22 (m, 5H) ppm.

Preparation 184 tert-Butyl ({(5S)-5-[(benzyloxy)methyl]tetrahydro-2H-pyran-2-yl}methyl) carbamate The reaction mixture of ({(5S)-5-[(benzyloxy)methyl]tetrahydro-2H-pyran-2- yl} methyl) amine (5. 51g, 21. 1 mmol), Boc2O (5.06 g, 23.2 mmiol) and Et3N (8.82 mL, 63.3 mmol) in CH2C12 was stirred at room temperature overnight. The mixture was diluted with CH2Cl2 and was washed with sat. NaHCO3aq and brine. The organic layer was dried over Na2S04, filtered and evaporated. The crude product was purified by silica gel column chromatography (hexane-AcOEt 9: 1-17: 3) to give the titled compound (7.58 g, 22.6 mmol).

1H NMR (CDC13) 8 : 7.39-7. 24 (m, 5H), 4.98-4. 86 (m, 1H), 4.60-4. 40 (m, 2H), 4.12-3. 92 (m, 1H), 3.70-3. 08 (m, 5H), 3.06-2. 86 (m, 1H), 2.00-1. 18 (m, 14H) ppm.

Preparation 185 tert-Butyl f [(ss)-5-(hvdroxymethyl) tetrahydro-2H-pyran-2-yllmethylWcarbamate This compound was prepared with tert-butyl_ ({(5S)-5-[(benzyloxy) methyl] tetrahydro- 2H-pyran-2-yl} methyl) carbamate by a procedure similar to that in Preparation 3.

'H NMR (CDCl3) 8 : 4.95 (br, 1H), 4.12-4. 00 (m, 1H), 3.90-3. 65 (m, 1H), 3.60-3. 10 (m, 4H), 3.08-2. 92 (m, 1H), 1.93-1. 13 (m, 14H) ppm. (OH was not observed.) Preparation 186 2-r (4-Chlorophenoxy)methyl]hex-5-en-1-ol This compound was prepared with 2-but-3-en-1-ylpropane-1, 3-diol and 4-chlorophenol by a procedure similar to that in Preparation 104.

'H NMR (CDCl3) # : 7.26-7. 17 (m, 2H), 6. 87-6. 74 (m, 2H), 5.92-5. 72 (m, 1H), 5.09-4. 97 (m, 2H), 4.04-3. 94 (m, 2H), 3. 87-3. 65 (m, 2H), 2.20-2. 00 (m, 3H), 1.65-1. 45 (m, 2H) ppm. (OH was not observed.) Preparation 187 2-f (4-Chlorophenoxv) methyll-4-oxiran-2ylbutan-1-ol This compound was prepared with 2-[(4-chlorophenoxy)methyl]hex-5-en-1-ol by a procedure similar to that in Preparation 40.

'H NMR (CDCI3) 8 : 7.32-7. 15 (m, 2H), 6.90-6. 72 (m, 2H), 4.06-3. 96 (m, 2H), 3.85-3. 66 (m, 2H), 3.00-2. 90 (m, 1H), 2.78 (t, J=4. 5 Hz, 1H), 2.13-1. 96 (m, 1H) 1.94-1. 50 (m, 5H) ppm.

(OH was not observed.) Preparation 188 f 5-f (4-Chlorophenoxy) methylltetrahYdro-2H-pYran-2-yl} methanol This compound was prepared with 2- [ (4-chlorophenoxy) methyl]-4-oxiran-2-ylbutan-l-ol by a procedure similar to that in Preparation 109.

'H NMR (CDC13) 8 : 7.30-7. 19 (m, 2H), 6.90-6. 76 (m, 2H), 4.25-3. 24 (m, 7H), 2. 14-1. 34 (m, 5H) ppm. (-OH was not observed.) Preparation 189 2-(Azidomethyll-S-F (4-chlorophenoxv) methylltetrahydro-2H-pyran This compound was prepared with {5-[(4-chlorophenoxy)methyl]tetrahydro-2H-pyran-2- yl} methanol by a procedure similar to that in Preparation 67.

'H NMR (CDCl3) 8 : 7.40-7. 17 (m, 2H), 6.95-6. 72 (m, 2H), 4.23-3. 43 (m, 5H), 3.37-3. 18 (m, 2H), 2.23-1. 92 (m, 2H), 1.90-1. 19 (m, 3H) ppm.

Experimental Example NR2B Binding Assay and Human Dofetilide Binding Assay were conducted using the method described above. The results of these studies are summarized in Table 1.

Table 1. Results of NR2B Binding Assay and Human Dofetilide Binding Assay )---------j----------------- !------------j-----------j Compound Structure NR2B Binding Dofetilide Bi IC50 (nM) IC50 (uM) E ample 9 7. 5 26. 3 fTTl Example 1 j aH 12. 0 25. 8 K kd . Example 11 1°l ON 8. S >100 a." NOm + '' Example 13 BO'O62 s Y Example 15JiM2020 ? 7 ~"e NOX +O, vCI v Example 16 19. 8 >100 t a Example e 17 0 23, 4 >100 o _ .. A k°yL Example 18 ! Oft 15. 2 >100 mY HOt ; J WOvox Example 19 13K 7. 7 >100 HO AQn A kJ-'Y Example 21 11. 4 >100 fr' HO Eample 22 ° nH 11. 0. >100 f .. _ Example 23 ° oN 28. 6 >100 « e O M-A k. f Example 24 0 0 H 21. 8 97. 0 M'' 4 Example 30 0 0"23. 6 >100 "u. f ruz Example 33 F/19. 3 95. 6 cul N cr Example 3 f 0 N C'14. 7 >30 I f ,.,.'., b w 1 A k\J' Example 35 20. 9 51. 6 0 N o Example 3f 7. 5 55. 2 o I N 0 Example 35 9 8. 2 >100 I I 0 Example 40'"Yit fl'Y) - 0 Example 4"° N ., w^, o .. 10. 4 >100 ,. J 0 Example 43 ° 7. 6 >100 1 O " N/'n'/ nu Example 48 il o 25. 5 >100 _ .)'j0. j0 J Example 50 0 27. 5 >100 Oh Example 5 0 OH 30. 2 >100 ,, f Example 6 ° oH 18. 2 >100 . Example 62 OH 9. 1 >30 . 13 HO Y Example 63 F O N 6. 2 >30 HO X m/ _ 0OB 26. 5 >100 Bv olple Gs g3Jt 26. 5 >100 w 0 Example 65 ° OH 10. 0 >100 HOeXOt O Example 66 0OH 12. 6 >100 infl HO 4 4 g ru Example 67 16. 9 >30 < " H Example 68 3 oH 7. 3 >100 fT""TY fj I f l Example 69 O) e OH 13. 2 >100 N P Ho I i Ho I F Example 710 7. 1 >100 NS <~JO _ kAJ' Example 73 15. 4 >30 h 0_ r Example 74 7. 2 54. 7 , a.. ,. Example 75 ° °"'6. 2 26. 8 HN a Example 76 29. 3 27. 7 HOU Wuo O_ yy_ Example 78 < 5. 4 40. 8 Ho, e vs Example 8 F ° 4N F 8. 9 >100 F Ho) en Example 81'535 ? 7 Hod r o Example 89 F 0 9. 0 >100 M Example 90 52>100 > Example 9I HO 13. 0 >30 \ M f10'. r' F Example 95 4. 0 >100 b0 t~ Example 105 27. 2 >100 \ 0e O N Example 106 16. 0 29. 1 NrwN v ! I 1 p F N lrlrr I Example 108"18>100 . H. A k° Example 113Jj65>100 'Tl fT' Example 114 7. 8 99. 4 K r « Example 116 25. 9 >100 . .- (Y-Q Example 12C 12. 2 >30 p 0, ey v -on " Example 123 7. 1 >100 at. N r i Example 125 Chiral 12. 5 >30 1 N Example 126 ral 18. 1 >100 TY" "T Example 129 10. 3 >30 Choral " IC50 : the concentration of the individual compound required to reduce the amount of ligand by 50%.