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Title:
TISSUE DISSOCIATION SOLUTION FOR ARABIDOPSIS THALIANA LEAF SAMPLE AND DISSOCIATION METHOD
Document Type and Number:
WIPO Patent Application WO/2024/077899
Kind Code:
A1
Abstract:
Provided are a tissue dissociation solution for an Arabidopsis thaliana leaf sample and a dissociation method. The tissue dissociation solution is prepared by means of adding 1%-3% (g/mL) of cellulase and 1%-3% (g/mL) of pectinase to 8%-10% (g/mL) of a mannitol solution. When the tissue dissociation solution is used for dissociating the Arabidopsis thaliana leaf sample, the yield of protoplast of the Arabidopsis thaliana leaves can be increased and the percentage of chloroplasts can be reduced.

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Inventors:
WANG CUITING (CN)
ZHANG ZHIMING (CN)
XIAO YUNPING (CN)
Application Number:
PCT/CN2023/087103
Publication Date:
April 18, 2024
Filing Date:
April 07, 2023
Export Citation:
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Assignee:
SHANGHAI OUYI BIOMEDICAL TECH CO LTD (CN)
International Classes:
C12N5/04
Foreign References:
CN115584338A2023-01-10
CN113717922A2021-11-30
CN114058564A2022-02-18
CN112680399A2021-04-20
US20220007607A12022-01-13
Other References:
YANG CHENGLAN; WU XIONGXIONG; QI CUNYING; LI XINYIN; ZHANG QIANQIAN; DUAN RUIJUN: "Optimization of Protoplast Preparation System of Hordeum Vulgare L.var.nudum.leaves", JOURNAL OF QINGHAI UNIVERSITY, vol. 39, no. 4, 20 August 2021 (2021-08-20), pages 26 - 30, XP009554115, ISSN: 1006-8996, DOI: 10.13901/j.cnki.qhwxxbzk.2021.04.004
GANESHAN SIVANANDHAN: "Optimization of Protoplast Isolation from Leaf Mesophylls of Chinese Cabbage (Brassica rapa ssp. pekinensis) and Subsequent Transfection with a Binary Vector", PLANTS, vol. 10, no. 12, 30 November 2021 (2021-11-30), pages 2636, XP093160265, ISSN: 2223-7747, DOI: 10.3390/plants10122636
Attorney, Agent or Firm:
SHANGHAI YUTENG PATENT AGENCY (GENERAL PARTNERSHIP) et al. (CN)
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