Title:
TRANSAMINASE AND APPLICATION THEREOF IN PREPARING OPTICALLY PURE CHIRAL AMINE
Document Type and Number:
WIPO Patent Application WO/2022/143652
Kind Code:
A1
Abstract:
Provided are a transaminase and an application thereof in preparing an optically pure chiral amine. Compared to the amino acid sequence shown by SEQ ID NO: 1, the amino acid sequence of the transaminase has a substitution mutation at one or more positions among position 136, position 282, and position 306, and amino acid residues at the remaining positions are the same as SEQ ID NO: 1. Further provided is a method for using said transaminase to prepare a chiral amine. Utilization of the present transaminase can improve conversion rate.
Inventors:
LI JIE (CN)
SUN FENGLAI (CN)
TANG DALIN (CN)
ZHU DAILIAN (CN)
ZHENG CHENKANG (CN)
ZHU JINGYANG (CN)
SUN FENGLAI (CN)
TANG DALIN (CN)
ZHU DAILIAN (CN)
ZHENG CHENKANG (CN)
ZHU JINGYANG (CN)
Application Number:
PCT/CN2021/142048
Publication Date:
July 07, 2022
Filing Date:
December 28, 2021
Export Citation:
Assignee:
SHANGHAI STA PHARMACEUTICAL R&D CO LTD (CN)
SHANGHAI SYNTHEALL PHARMACEUTICALS CO LTD (CN)
SHANGHAI SYNTHEALL PHARMACEUTICALS CO LTD (CN)
International Classes:
C12P13/00; C40B50/06
Foreign References:
| CN104480155A | 2015-04-01 | |||
| CN110551771A | 2019-12-10 | |||
| US20020192786A1 | 2002-12-19 | |||
| CN108660122A | 2018-10-16 | |||
| CN104894148A | 2015-09-09 |
Other References:
DATABASE Protein 1 December 2020 (2020-12-01), ANONYMOUS: "Chain L, (R)-amine transaminase", XP055947710, retrieved from Genbank Database accession no. 3WWI_L
GUAN LI-JUN, OHTSUKA JUN, OKAI MASAHIKO, MIYAKAWA TAKUYA, MASE TOMOKO, ZHI YUEHUA, HOU FENG, ITO NORIYUKI, IWASAKI AKIRA, YASOHARA: "A new target region for changing the substrate specificity of amine transaminases", SCIENTIFIC REPORTS, vol. 5, no. 1, 1 September 2015 (2015-09-01), pages 1 - 11, XP055825479, DOI: 10.1038/srep10753
GUAN LI-JUN, OHTSUKA JUN, OKAI MASAHIKO, MIYAKAWA TAKUYA, MASE TOMOKO, ZHI YUEHUA, HOU FENG, ITO NORIYUKI, IWASAKI AKIRA, YASOHARA: "A new target region for changing the substrate specificity of amine transaminases", SCIENTIFIC REPORTS, vol. 5, no. 1, 1 September 2015 (2015-09-01), pages 1 - 11, XP055825479, DOI: 10.1038/srep10753
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
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