Title:
TRANSCRIPT OPTIMIZED EXPRESSION ENHANCEMENT FOR HIGH-LEVEL PRODUCTION OF PROTEINS AND PROTEIN DOMAINS
Document Type and Number:
WIPO Patent Application WO/2013/071295
Kind Code:
A3
Abstract:
The present invention relates to a system for high-level production of recombinant proteins and protein domains.
Inventors:
ACTON THOMAS B (US)
ANDERSON STEPHEN (US)
HUANG YUANPENG JANET (US)
MONTELIONE GAETANO (US)
ANDERSON STEPHEN (US)
HUANG YUANPENG JANET (US)
MONTELIONE GAETANO (US)
Application Number:
PCT/US2012/064836
Publication Date:
July 18, 2013
Filing Date:
November 13, 2012
Export Citation:
Assignee:
UNIV RUTGERS (US)
ACTON THOMAS B (US)
ANDERSON STEPHEN (US)
HUANG YUANPENG JANET (US)
MONTELIONE GAETANO (US)
ACTON THOMAS B (US)
ANDERSON STEPHEN (US)
HUANG YUANPENG JANET (US)
MONTELIONE GAETANO (US)
International Classes:
C12N15/67
Domestic Patent References:
| WO2006097945A2 | 2006-09-21 | |||
| WO1989000604A1 | 1989-01-26 |
Other References:
HUANG XU ET AL: "Directed evolution of the 5 '-untranslated region of the phoA gene in Escherichia coli simultaneously yields a stronger promoter and a stronger Shine-Dalgarno sequence", November 2006, BIOTECHNOLOGY JOURNAL, VOL. 1, NR. 11, PAGE(S) 1275-1282, ISSN: 1860-6768, XP002696741
PAIK SOON-YOUNG ET AL: "The influence of the nucleotide sequences of random Shine-Dalgarno and spacer region on bovine growth hormone gene expression", THE JOURNAL OF MICROBIOLOGY, HAN-GUG MISAENGMUL HAG-HOE, KOREA, REPUBLIC OF, vol. 44, no. 1, 1 February 2006 (2006-02-01), pages 64 - 71, XP002497576, ISSN: 1225-8873
JIA M ET AL: "The relationship among gene expression, folding free energy and codon usage bias in Escherichia coli", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 579, no. 24, 10 October 2005 (2005-10-10), pages 5333 - 5337, XP027697304, ISSN: 0014-5793, [retrieved on 20051010]
ACTON THOMAS B ET AL: "Preparation of protein samples for NMR structure, function, and small-molecule screening studies", METHODS IN ENZYMOLOGY, ACADEM. PRESS, USA, vol. 493, 1 January 2011 (2011-01-01), pages 21 - 60, XP009169372, ISSN: 1557-7988, DOI: 10.1016/B978-0-12-381274-2.00002-9
PAIK SOON-YOUNG ET AL: "The influence of the nucleotide sequences of random Shine-Dalgarno and spacer region on bovine growth hormone gene expression", THE JOURNAL OF MICROBIOLOGY, HAN-GUG MISAENGMUL HAG-HOE, KOREA, REPUBLIC OF, vol. 44, no. 1, 1 February 2006 (2006-02-01), pages 64 - 71, XP002497576, ISSN: 1225-8873
JIA M ET AL: "The relationship among gene expression, folding free energy and codon usage bias in Escherichia coli", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 579, no. 24, 10 October 2005 (2005-10-10), pages 5333 - 5337, XP027697304, ISSN: 0014-5793, [retrieved on 20051010]
ACTON THOMAS B ET AL: "Preparation of protein samples for NMR structure, function, and small-molecule screening studies", METHODS IN ENZYMOLOGY, ACADEM. PRESS, USA, vol. 493, 1 January 2011 (2011-01-01), pages 21 - 60, XP009169372, ISSN: 1557-7988, DOI: 10.1016/B978-0-12-381274-2.00002-9
Attorney, Agent or Firm:
KRUEGER, Katherine A.D. et al. (Suite 670Bloomington, Minnesota, US)
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