Title:
TRANSFERABLE TYPE I-F CRISPR-CAS GENOME EDITING SYSTEM
Document Type and Number:
WIPO Patent Application WO/2022/095929
Kind Code:
A1
Abstract:
A generic type I CRISPR-Cas-based genome-editing system that can be used in microbial hosts having diverse genetic backgrounds has been established. The chromosomal integration system overcomes the limitations of narrow host range and the requirement for antibiotics to maintain propagation and expression which are associated with plasmid-encoded Cas proteins. Compositions and methods for a chromosomal integrated type I-F CRISPR-Cas system for programmable genome editing and robust gene regulation are provided. In some embodiments, the compositions and methods are effective to selectively and specifically edit and/or regulate the genome of multiple microbial species with diverse genotypes. Compositions and methods for gene editing and/or gene regulation in Pseudomonas spp, such as multiple strains of P. aeruginosa, are described.
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Inventors:
YAN AIXIN (CN)
XU ZELING (CN)
XU ZELING (CN)
Application Number:
PCT/CN2021/128715
Publication Date:
May 12, 2022
Filing Date:
November 04, 2021
Export Citation:
Assignee:
UNIV HONG KONG (CN)
International Classes:
C12N15/90; A01H5/00; A01K67/027; C12M1/00; C12N1/21; C12N15/10
Domestic Patent References:
| WO2018229521A1 | 2018-12-20 | |||
| WO2019075159A1 | 2019-04-18 | |||
| WO2017019867A1 | 2017-02-02 |
Foreign References:
| CN111979240A | 2020-11-24 | |||
| CN111492059A | 2020-08-04 | |||
| US20200140887A1 | 2020-05-07 | |||
| CN111615557A | 2020-09-01 |
Attorney, Agent or Firm:
LIU, SHEN & ASSOCIATES (CN)
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