Title:
TRANSFORMANT AND METHOD FOR PRODUCING CAROTENOID COMPOSITION USING SAME
Document Type and Number:
WIPO Patent Application WO/2022/102595
Kind Code:
A1
Abstract:
The present invention provides a technique for synthesizing a carotenoid composition by a genetic recombination technique. A transformant in which a first promoter, an upstream gene of a carotenoid biosynthesis gene including a crtY and crtZ operably linked to the first promoter, a second promoter having higher promoter intensity than the first promoter, and a ZEP gene and a CCS gene operably linked to the second promoter have been introduced into a host cell.
Inventors:
KUBO AKIKO (JP)
OHDAN KOHJI (JP)
TERADA YOSHINOBU (JP)
YAOI KATSURO (JP)
FURUBAYASHI MAIKO (JP)
MISAWA NORIHIKO (JP)
KOBAYASHI MIHO (JP)
HATTAN JUNICHIRO (JP)
MAOKA TAKASHI (JP)
OHDAN KOHJI (JP)
TERADA YOSHINOBU (JP)
YAOI KATSURO (JP)
FURUBAYASHI MAIKO (JP)
MISAWA NORIHIKO (JP)
KOBAYASHI MIHO (JP)
HATTAN JUNICHIRO (JP)
MAOKA TAKASHI (JP)
Application Number:
PCT/JP2021/041098
Publication Date:
May 19, 2022
Filing Date:
November 09, 2021
Export Citation:
Assignee:
EZAKI GLICO CO (JP)
International Classes:
C12N1/21; C12N1/15; C12N1/19; C12N5/10; C12N15/52; C12N15/53; C12P23/00
Foreign References:
| JPH09510608A | 1997-10-28 | |||
| US20140380516A1 | 2014-12-25 | |||
| JP2008532492A | 2008-08-21 |
Other References:
JEKNIĆ ZORAN, MORRÉ JEFFREY T., JEKNIĆ STEVAN, JEVREMOVIĆ SLAĐANA, SUBOTIĆ ANGELINA, CHEN TONY H.H.: "Cloning and Functional Characterization of a Gene for Capsanthin-Capsorubin Synthase from Tiger Lily (Lilium lancifolium Thunb. ‘Splendens’)", PLANT AND CELL PHSIOLOGY, OXFORD UNIVERSITY PRESS, UK, vol. 53, no. 11, 1 November 2012 (2012-11-01), UK , pages 1899 - 1912, XP055929969, ISSN: 0032-0781, DOI: 10.1093/pcp/pcs128
KUMAGAI MONTO H., YVES KELLER, FLORENCE BOUVIER, DAVID CLARY, BILAL CAMARA: "Functional integration of non-native carotenoids into chloroplasts by viral-derived expression of capsanthin-capsorubin synthase in Nicotiana benthamiana", THE PLANT JOURNAL, vol. 14, no. 3, 31 May 1998 (1998-05-31), pages 305 - 315, XP055929968, DOI: 10.1046/j.1365-313X.1998.00128.x
FURUBAYASHI MAIKO, KUBO AKIKO, TAKEMURA MIHO, OTANI YUKO, MAOKA TAKASHI, TERADA YOSHINOBU, YAOI KATSURO, OHDAN KOHJI, MISAWA NORIH: "Capsanthin Production in Escherichia coli by Overexpression of Capsanthin/Capsorubin Synthase from Capsicum annuum", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 69, no. 17, 5 May 2021 (2021-05-05), US , pages 5076 - 5085, XP055929965, ISSN: 0021-8561, DOI: 10.1021/acs.jafc.1c00083
EDITHH.M. LEMPENS, INGRID VANBAAL, JOOSTL.J. VANDONGEN, TILMANM. HACKENG, : "Noncovalent Synthesis of Protein Dendrimers", CHEMISTRY - A EUROPEAN JOURNAL, JOHN WILEY & SONS, INC, DE, vol. 15, no. 35, 7 September 2009 (2009-09-07), DE, pages 8760 - 8767, XP055349052, ISSN: 0947-6539, DOI: 10.1002/chem.200900748
ROHMER, M. ET AL., BIOCHEM. J., vol. 295, 1993, pages 517
KAJIWARA, S. ET AL., BIOCHEM. J., vol. 324, 1997, pages 421
TAKEMURA, M. ET AL., TETRAHEDRON LETTERS, vol. 56, 2015, pages 6063
KAKINUMA, K. ET AL., J. AM. CHEM. SOC., vol. 123, 2001, pages 1238
JOURNAL OF THE SOCIETY FOR BIOTECHNOLOGY, vol. 93, no. 7, 2015
BOUVIER, F. ET AL., THE PLANT JOURNAL, vol. 6, no. 1, 1994, pages 45
PBLA UNITDEUSCHLE ET AL., EMBO J., vol. 5, 1986, pages 2987 - 2994
LANZER ET AL., PNAS USA, vol. 85, 1988, pages 8973 - 8977
MEYER ET AL., NATURE CHEMICAL BIOLOGY, vol. 15, 2019, pages 196 - 204
LOPEZ-ALONSO, J.P.BRUIX, M.FONT, J.RIBO, M.VILANOVA, M.RICO, M.GOTTE, G.LIBONATI, M.GONZALEZ, C.LAURENTS, D.V., J.BIOL.CHEM., vol. 281, 2006, pages 9400 - 9406
TATIANA A. TATSUSOVATHOMAS L. MADDEN, FEMS MICROBIOL. LETT., vol. 174, 1999, pages 247 - 250
FURUBAYASHI, M.KUBO, A.TAKEMURA, M.OTANI, Y.MAOKA, T.TERADA, Y.YAOI, K.OHDAN, K.MISAWA, N.MITANI, Y., J.AGRIC.FOOD CHEM., vol. 69, 2021, pages 5076 - 5085
KUMAGAI MONTO H., YVES KELLER, FLORENCE BOUVIER, DAVID CLARY, BILAL CAMARA: "Functional integration of non-native carotenoids into chloroplasts by viral-derived expression of capsanthin-capsorubin synthase in Nicotiana benthamiana", THE PLANT JOURNAL, vol. 14, no. 3, 31 May 1998 (1998-05-31), pages 305 - 315, XP055929968, DOI: 10.1046/j.1365-313X.1998.00128.x
FURUBAYASHI MAIKO, KUBO AKIKO, TAKEMURA MIHO, OTANI YUKO, MAOKA TAKASHI, TERADA YOSHINOBU, YAOI KATSURO, OHDAN KOHJI, MISAWA NORIH: "Capsanthin Production in Escherichia coli by Overexpression of Capsanthin/Capsorubin Synthase from Capsicum annuum", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 69, no. 17, 5 May 2021 (2021-05-05), US , pages 5076 - 5085, XP055929965, ISSN: 0021-8561, DOI: 10.1021/acs.jafc.1c00083
EDITHH.M. LEMPENS, INGRID VANBAAL, JOOSTL.J. VANDONGEN, TILMANM. HACKENG, : "Noncovalent Synthesis of Protein Dendrimers", CHEMISTRY - A EUROPEAN JOURNAL, JOHN WILEY & SONS, INC, DE, vol. 15, no. 35, 7 September 2009 (2009-09-07), DE, pages 8760 - 8767, XP055349052, ISSN: 0947-6539, DOI: 10.1002/chem.200900748
ROHMER, M. ET AL., BIOCHEM. J., vol. 295, 1993, pages 517
KAJIWARA, S. ET AL., BIOCHEM. J., vol. 324, 1997, pages 421
TAKEMURA, M. ET AL., TETRAHEDRON LETTERS, vol. 56, 2015, pages 6063
KAKINUMA, K. ET AL., J. AM. CHEM. SOC., vol. 123, 2001, pages 1238
JOURNAL OF THE SOCIETY FOR BIOTECHNOLOGY, vol. 93, no. 7, 2015
BOUVIER, F. ET AL., THE PLANT JOURNAL, vol. 6, no. 1, 1994, pages 45
PBLA UNITDEUSCHLE ET AL., EMBO J., vol. 5, 1986, pages 2987 - 2994
LANZER ET AL., PNAS USA, vol. 85, 1988, pages 8973 - 8977
MEYER ET AL., NATURE CHEMICAL BIOLOGY, vol. 15, 2019, pages 196 - 204
LOPEZ-ALONSO, J.P.BRUIX, M.FONT, J.RIBO, M.VILANOVA, M.RICO, M.GOTTE, G.LIBONATI, M.GONZALEZ, C.LAURENTS, D.V., J.BIOL.CHEM., vol. 281, 2006, pages 9400 - 9406
TATIANA A. TATSUSOVATHOMAS L. MADDEN, FEMS MICROBIOL. LETT., vol. 174, 1999, pages 247 - 250
FURUBAYASHI, M.KUBO, A.TAKEMURA, M.OTANI, Y.MAOKA, T.TERADA, Y.YAOI, K.OHDAN, K.MISAWA, N.MITANI, Y., J.AGRIC.FOOD CHEM., vol. 69, 2021, pages 5076 - 5085
Attorney, Agent or Firm:
TANAKA, Junya et al. (JP)
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